WO2002040632A3 - Creation and identification of proteins having new dna binding specificities - Google Patents

Creation and identification of proteins having new dna binding specificities Download PDF

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Publication number
WO2002040632A3
WO2002040632A3 PCT/US2001/043107 US0143107W WO0240632A3 WO 2002040632 A3 WO2002040632 A3 WO 2002040632A3 US 0143107 W US0143107 W US 0143107W WO 0240632 A3 WO0240632 A3 WO 0240632A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna binding
identification
proteins
methods
dna
Prior art date
Application number
PCT/US2001/043107
Other languages
French (fr)
Other versions
WO2002040632A2 (en
Inventor
John G Wise
Katja Fromknecht
Original Assignee
John G Wise
Katja Fromknecht
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by John G Wise, Katja Fromknecht filed Critical John G Wise
Priority to AU2002225623A priority Critical patent/AU2002225623A8/en
Priority to US10/416,708 priority patent/US20040161753A1/en
Priority to AU2002225623A priority patent/AU2002225623A1/en
Publication of WO2002040632A2 publication Critical patent/WO2002040632A2/en
Publication of WO2002040632A3 publication Critical patent/WO2002040632A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Abstract

Methods are provided for identification and production of new DNA binding proteins that up or down regulate the expression of pre-determined target genes. Such genes include DNA sequences that encode proteins that regulate such target genes as well as gene constructs and biological materials that contain such DNA binding proteins and/or their DNA sequences. Discovery methods also are provided for transcriptional promoters that allow identification of the desired target gene specific DNA binding proteins, methods for targeting DNA binding protein variants to the desired DNA binding sequence, the methods for removing undesired DNA binding protein variants from the total pool of all variants, as well as the media used for assaying in vivo DNA binding. The invention further encompasses kits for the identification and production of DNA binding protein variants and/or their DNA sequences.
PCT/US2001/043107 2000-11-17 2001-11-16 Creation and identification of proteins having new dna binding specificities WO2002040632A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002225623A AU2002225623A8 (en) 2000-11-17 2001-11-16 Creation and identification of proteins having new dna binding specificities
US10/416,708 US20040161753A1 (en) 2001-11-16 2001-11-16 Creation and identification of proteins having new dna binding specificities
AU2002225623A AU2002225623A1 (en) 2000-11-17 2001-11-16 Creation and identification of proteins having new dna binding specificities

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24954600P 2000-11-17 2000-11-17
US60/249,546 2000-11-17

Publications (2)

Publication Number Publication Date
WO2002040632A2 WO2002040632A2 (en) 2002-05-23
WO2002040632A3 true WO2002040632A3 (en) 2009-06-11

Family

ID=22943953

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/043107 WO2002040632A2 (en) 2000-11-17 2001-11-16 Creation and identification of proteins having new dna binding specificities

Country Status (2)

Country Link
AU (2) AU2002225623A1 (en)
WO (1) WO2002040632A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747253A (en) * 1991-08-23 1998-05-05 Isis Pharmaceuticals, Inc. Combinatorial oligomer immunoabsorbant screening assay for transcription factors and other biomolecule binding
US5773218A (en) * 1992-01-27 1998-06-30 Icos Corporation Method to identify compounds which modulate ICAM-related protein interactions
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5955280A (en) * 1995-04-11 1999-09-21 The General Hospital Corporation Reverse two-hybrid system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747253A (en) * 1991-08-23 1998-05-05 Isis Pharmaceuticals, Inc. Combinatorial oligomer immunoabsorbant screening assay for transcription factors and other biomolecule binding
US5773218A (en) * 1992-01-27 1998-06-30 Icos Corporation Method to identify compounds which modulate ICAM-related protein interactions
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5955280A (en) * 1995-04-11 1999-09-21 The General Hospital Corporation Reverse two-hybrid system

Also Published As

Publication number Publication date
AU2002225623A1 (en) 2002-05-27
AU2002225623A8 (en) 2009-07-30
WO2002040632A2 (en) 2002-05-23

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