WO2002031206A3 - Methods and compositions using hybridization assays for detecting infectious agents - Google Patents

Methods and compositions using hybridization assays for detecting infectious agents Download PDF

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Publication number
WO2002031206A3
WO2002031206A3 PCT/US2001/031829 US0131829W WO0231206A3 WO 2002031206 A3 WO2002031206 A3 WO 2002031206A3 US 0131829 W US0131829 W US 0131829W WO 0231206 A3 WO0231206 A3 WO 0231206A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
detecting
compositions
infectious agents
hybridization assays
Prior art date
Application number
PCT/US2001/031829
Other languages
French (fr)
Other versions
WO2002031206A2 (en
Inventor
William L Ragland
Renata Novak
Original Assignee
William L Ragland
Renata Novak
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William L Ragland, Renata Novak filed Critical William L Ragland
Priority to AU2002213129A priority Critical patent/AU2002213129A1/en
Publication of WO2002031206A2 publication Critical patent/WO2002031206A2/en
Publication of WO2002031206A3 publication Critical patent/WO2002031206A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

A method for detecting the presence of target nucleic acid sequences in a human or animal subject infected by an infectious agent comprising obtaining a fluid or solubilized sample from the subject and detecting the presence of the target in the sample by nucleic acid hybridization. In addition, the present invention is directed to methods of detecting and measuring any oligonucleotides and polynucleotides of interest, including but not limited to mRNA for cytokines and hormones and genetic DNA sequences.
PCT/US2001/031829 2000-10-11 2001-10-11 Methods and compositions using hybridization assays for detecting infectious agents WO2002031206A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002213129A AU2002213129A1 (en) 2000-10-11 2001-10-11 Methods and compositions using hybridization assays for detecting infectious agents

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68630300A 2000-10-11 2000-10-11
US09/686,303 2000-10-11

Publications (2)

Publication Number Publication Date
WO2002031206A2 WO2002031206A2 (en) 2002-04-18
WO2002031206A3 true WO2002031206A3 (en) 2003-07-31

Family

ID=24755754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031829 WO2002031206A2 (en) 2000-10-11 2001-10-11 Methods and compositions using hybridization assays for detecting infectious agents

Country Status (2)

Country Link
AU (1) AU2002213129A1 (en)
WO (1) WO2002031206A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004097048A1 (en) * 2003-04-28 2004-11-11 Canon Kabushiki Kaisha Process for assay of nucleic acid by competitive hybridization using a dna microarray
JP2005031066A (en) * 2003-04-28 2005-02-03 Canon Inc Processing method of immobilized nucleic acid probe, method for detecting nucleic acid, method for analyzing nucleic acid density, and kit for detecting nucleic acid
WO2008055915A2 (en) * 2006-11-06 2008-05-15 Clondiag Gmbh Device and process for assays using binding members
AU2016202243B2 (en) * 2006-11-06 2017-12-14 Clondiag Gmbh Assays
CN102586489B (en) * 2010-04-09 2013-09-18 山东农业大学 Detection of infection of avian anemia viruses in pathologic material by combination of polymerase chain reaction (PCR) and nucleic acid probe and dot blot hybridization technology
WO2021215989A1 (en) 2020-04-24 2021-10-28 Aman Russom Rapid detection of specific genetic sequences using a multi-labelled dna hybrid comprising a reporter strand and an anchor strand
WO2023196528A1 (en) * 2022-04-08 2023-10-12 Illumina, Inc. Aptamer dynamic range compression and detection techniques

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5616465A (en) * 1995-08-09 1997-04-01 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using competitive hybridization probes
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US6127115A (en) * 1995-11-14 2000-10-03 Ragland; William L. Efficient method of detecting an infectious agent in blood

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5616465A (en) * 1995-08-09 1997-04-01 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using competitive hybridization probes
US6127115A (en) * 1995-11-14 2000-10-03 Ragland; William L. Efficient method of detecting an infectious agent in blood

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
NOVAK R ET AL: "Competitive DNA hybridization in microtitre plates for chicken anaemia virus.", MOLECULAR AND CELLULAR PROBES, vol. 15, no. 1, February 2001 (2001-02-01), pages 1 - 11, XP001147365, ISSN: 0890-8508 *
NOVAK R ET AL: "In situ hybridization for detection of chicken anaemia virus in peripheral blood smears.", MOLECULAR AND CELLULAR PROBES. ENGLAND APR 1997, vol. 11, no. 2, April 1997 (1997-04-01), pages 135 - 141, XP001147364, ISSN: 0890-8508 *
NOVAK RENATA ET AL: "Immune status assessment by abundance of IFN-alpha and IFN-gamma mRNA in chicken blood.", JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, vol. 21, no. 8, August 2001 (2001-08-01), pages 643 - 651, XP009009469, ISSN: 1079-9907 *
RUTENFRANZ I ET AL: "IN-SITU HYBRIDIZATION OF THE MRNA FOR INTERFERON-GAMMA INTERFERON-ALPHA-E INTERFERON-BETA INTERLEUKIN-1-BETA AND INTERLEUKIN-6 AND CHARACTERIZATION OF INFILTRATING CELLS IN THYROID TISSUES", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 148, no. 1-2, 1992, pages 233 - 242, XP009009663, ISSN: 0022-1759 *

Also Published As

Publication number Publication date
WO2002031206A2 (en) 2002-04-18
AU2002213129A1 (en) 2002-04-22

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