WO2002027035A3 - High-throughput gene cloning and phenotypic screening - Google Patents

High-throughput gene cloning and phenotypic screening Download PDF

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Publication number
WO2002027035A3
WO2002027035A3 PCT/US2001/030762 US0130762W WO0227035A3 WO 2002027035 A3 WO2002027035 A3 WO 2002027035A3 US 0130762 W US0130762 W US 0130762W WO 0227035 A3 WO0227035 A3 WO 0227035A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene cloning
robotically
phenotypic screening
recombination
throughput gene
Prior art date
Application number
PCT/US2001/030762
Other languages
French (fr)
Other versions
WO2002027035A2 (en
Inventor
David A Zarling
Ron Caspi
Kathryn M Stephens
Roy G Sergeant
Christopher Lehman
Sushma Pati
Original Assignee
Pangene Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pangene Corporation filed Critical Pangene Corporation
Priority to AU2001296471A priority Critical patent/AU2001296471A1/en
Publication of WO2002027035A2 publication Critical patent/WO2002027035A2/en
Publication of WO2002027035A3 publication Critical patent/WO2002027035A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to the use of high-throughput methods for gene targeting, recombination, phenotype screening and biovalidation of drug targets utilizing enhanced homologous recombination (EHR) techniques. These methods utilize robotically driven multichannel pipetters to perform liquid, particle, cell and organism handling, robotically controlled plate and sample handling platforms, magnetic probes and affinity probes to selectivily capture nucleic acid hybrids, and thermally regulated plates or blocks for temperature controlled reactions.
PCT/US2001/030762 2000-09-28 2001-09-28 High-throughput gene cloning and phenotypic screening WO2002027035A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001296471A AU2001296471A1 (en) 2000-09-28 2001-09-28 High-throughput gene cloning and phenotypic screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23641000P 2000-09-28 2000-09-28
US60/236,410 2000-09-28

Publications (2)

Publication Number Publication Date
WO2002027035A2 WO2002027035A2 (en) 2002-04-04
WO2002027035A3 true WO2002027035A3 (en) 2003-08-28

Family

ID=22889384

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/030762 WO2002027035A2 (en) 2000-09-28 2001-09-28 High-throughput gene cloning and phenotypic screening

Country Status (2)

Country Link
AU (1) AU2001296471A1 (en)
WO (1) WO2002027035A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2808118C (en) 2010-07-22 2016-04-19 Kieran Curran Composite liquid cells
CA2888042A1 (en) 2012-11-27 2014-06-05 Gencell Biosystems Ltd. Handling liquid samples
US10384187B2 (en) * 2014-02-10 2019-08-20 Gencell Biosystems Ltd Composite liquid cell (CLC) mediated nucleic acid library preparation device, and methods for using the same

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016675A1 (en) * 1990-04-06 1991-10-31 Applied Biosystems, Inc. Automated molecular biology laboratory
EP0718404A2 (en) * 1994-11-22 1996-06-26 Takara Shuzo Co. Ltd. Method for site-directed mutagenesis
WO1996023078A1 (en) * 1995-01-27 1996-08-01 Incyte Pharmaceuticals, Inc. Computer system storing and analyzing microbiological data
WO1998001760A2 (en) * 1996-07-05 1998-01-15 Beckman Coulter, Inc. Automated sample processing system
WO1998042727A1 (en) * 1997-03-21 1998-10-01 Sri International Sequence alterations using homologous recombination
WO1999026070A2 (en) * 1997-11-19 1999-05-27 Mwg-Biotech Ag Method for automatic implementation of chemical or biological methods
WO1999037755A2 (en) * 1997-12-11 1999-07-29 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
EP0995555A1 (en) * 1998-10-15 2000-04-26 Tecan AG Robot arm
WO2000056872A2 (en) * 1999-03-22 2000-09-28 Pangene Corporation High-throughput gene cloning and phenotypic screening

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016675A1 (en) * 1990-04-06 1991-10-31 Applied Biosystems, Inc. Automated molecular biology laboratory
EP0718404A2 (en) * 1994-11-22 1996-06-26 Takara Shuzo Co. Ltd. Method for site-directed mutagenesis
WO1996023078A1 (en) * 1995-01-27 1996-08-01 Incyte Pharmaceuticals, Inc. Computer system storing and analyzing microbiological data
WO1998001760A2 (en) * 1996-07-05 1998-01-15 Beckman Coulter, Inc. Automated sample processing system
WO1998042727A1 (en) * 1997-03-21 1998-10-01 Sri International Sequence alterations using homologous recombination
WO1999026070A2 (en) * 1997-11-19 1999-05-27 Mwg-Biotech Ag Method for automatic implementation of chemical or biological methods
WO1999037755A2 (en) * 1997-12-11 1999-07-29 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
EP0995555A1 (en) * 1998-10-15 2000-04-26 Tecan AG Robot arm
WO2000056872A2 (en) * 1999-03-22 2000-09-28 Pangene Corporation High-throughput gene cloning and phenotypic screening

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Roboamp4200 specifications", XP002147679, Retrieved from the Internet <URL:http://www.mwg-biotech.de/products/pcr/robots.> *
BATTERSBY B J ET AL: "Novel miniaturized systems in high-throughput screening", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 20, no. 4, 1 April 2002 (2002-04-01), pages 167 - 173, XP004344215, ISSN: 0167-7799 *
CLONTECHNIQUES, January 2000 (2000-01-01), XP002235331, Retrieved from the Internet <URL:www.clontech.com/archive/JAN00UPD/pdf/TECAN.pdf> [retrieved on 20030319] *
GERHARD M: "Automatisierung in der Forensischen Molekulargenetik", APPLICATION NOTE MWG BIOTECH AG, XX, XX, 1 January 1998 (1998-01-01), XX, pages 1, XP002147678 *
GERHARD M: "GENOMIC SERVICES; Automatisierung in forensischen Molekulargenetik: Einsatz des der Laborroutine", GESCHAFTSBERICHT MWG BIOTECH AG, XX, XX, 1 January 1998 (1998-01-01), XX, pages 1 - 2, XP002147677 *
HALL J M ET AL: "AN APPROACH TO HIGH-THROUGHPUT GENOTYPING", PCR METHODS AND APPLICATIONS,US,COLD SPRING HARBOR, NY, 1996, pages 781 - 790, XP000700236, ISSN: 1054-9803 *

Also Published As

Publication number Publication date
WO2002027035A2 (en) 2002-04-04
AU2001296471A1 (en) 2002-04-08

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