WO2002023154A3 - Aggregation-based assays - Google Patents

Aggregation-based assays Download PDF

Info

Publication number
WO2002023154A3
WO2002023154A3 PCT/US2001/022696 US0122696W WO0223154A3 WO 2002023154 A3 WO2002023154 A3 WO 2002023154A3 US 0122696 W US0122696 W US 0122696W WO 0223154 A3 WO0223154 A3 WO 0223154A3
Authority
WO
WIPO (PCT)
Prior art keywords
macroparticle
assay mixture
detection
aggregation
assays
Prior art date
Application number
PCT/US2001/022696
Other languages
French (fr)
Other versions
WO2002023154A2 (en
Inventor
Bhanu P Sharma
Original Assignee
Biometric Imaging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biometric Imaging Inc filed Critical Biometric Imaging Inc
Priority to AU2001275985A priority Critical patent/AU2001275985A1/en
Publication of WO2002023154A2 publication Critical patent/WO2002023154A2/en
Publication of WO2002023154A3 publication Critical patent/WO2002023154A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0092—Monitoring flocculation or agglomeration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A method for measuring aggregation by the detection of individual particles (30) is disclosed. The method is adaptable for screening compounds, detection of low levels of a target (e.g. small molecules, large molecules, cells, or microbes), competitive binding assays and other assays. If the assay mixture contains a targeted compound (18), the particulate substrate (20) will aggregate to form a macroparticle (30). One of the components of the assay mixture is labeled with an optically detectable label (14). The macroparticle (30) has an associated macroparticle optical signal which would be identified by optical analysis of the assay mixture.
PCT/US2001/022696 2000-09-13 2001-07-18 Aggregation-based assays WO2002023154A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001275985A AU2001275985A1 (en) 2000-09-13 2001-07-18 Aggregation-based assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66117800A 2000-09-13 2000-09-13
US09/661,178 2000-09-13

Publications (2)

Publication Number Publication Date
WO2002023154A2 WO2002023154A2 (en) 2002-03-21
WO2002023154A3 true WO2002023154A3 (en) 2003-07-17

Family

ID=24652526

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/022696 WO2002023154A2 (en) 2000-09-13 2001-07-18 Aggregation-based assays

Country Status (2)

Country Link
AU (1) AU2001275985A1 (en)
WO (1) WO2002023154A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4299597B2 (en) * 2002-07-29 2009-07-22 シスメックス株式会社 Hematology analyzer and method
CA2719004C (en) 2008-03-21 2013-06-18 Abbott Point Of Care, Inc. Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample
ES2392380T3 (en) 2008-03-21 2012-12-10 Abbott Point Of Care, Inc. Procedure and apparatus for determining the hematocrit of a blood sample using the intrinsic pigmentation of the hemoglobin contained in the red blood cells
US8081303B2 (en) 2008-03-21 2011-12-20 Abbott Point Of Care, Inc. Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
ES2464572T3 (en) 2008-03-21 2014-06-03 Abbott Point Of Care, Inc. Method and apparatus for determining red blood cell indices in a blood sample using intrinsic pigmentation of hemoglobin contained in red blood cells
CN102027369B (en) 2008-03-21 2018-10-26 艾博特健康公司 Individually and in polymerizeing grumeleuse detect the method and apparatus with platelet Counting
CN102027370B (en) 2008-04-02 2016-06-08 艾博特健康公司 For the biofluid including whole blood being performed incorporation of markings in the ligand analysis of immunoassay and the virtual separation of free label
JP5734838B2 (en) 2008-04-09 2015-06-17 アボット ポイント オブ ケア インコーポレイテッド Method for measuring the area of a sample placed in an analysis chamber
WO2011082342A1 (en) 2009-12-31 2011-07-07 Abbott Point Of Care, Inc. Method and apparatus for determining mean cell volume of red blood cells
WO2011116305A1 (en) 2010-03-18 2011-09-22 Abbott Point Of Care, Inc. Method and apparatus for optically determining at least one hemoglobin related parameter of a whole blood sample
CA2995204A1 (en) 2015-08-10 2017-02-16 Essenlix Corp. Bio/chemical assay devices and methods for simplified steps, small samples, accelerated speed, and ease-of-use
US11648551B2 (en) 2017-12-12 2023-05-16 Essenlix Corporation Sample manipulation and assay with rapid temperature change
US11510608B2 (en) 2017-12-14 2022-11-29 Essenlix Corporation Devices, systems, and methods for monitoring hair
US11709162B2 (en) 2018-08-16 2023-07-25 Essenlix Corporation Homogeneous assay with particle aggregation or de-aggregation
US11879830B2 (en) 2018-09-28 2024-01-23 Arizona Board Of Regents On Behalf Of The University Of Arizona Quantitative large area binding sensor for detecting biomarkers
US20220137038A1 (en) * 2019-02-06 2022-05-05 Essenlix Corporation Assays with reduced interference (iii)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489537A (en) * 1991-12-13 1996-02-06 Bainbridge Sciences, Inc. Agglutination assays and kits employing colloidal dyes
US5491095A (en) * 1993-05-26 1996-02-13 Westinghouse Electric Corp. System and method for aggulutination detection
US5798215A (en) * 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
USRE36054E (en) * 1992-12-07 1999-01-19 Xylum Corporation Disposable cartridge for investigating physical properties of blood
US5939021A (en) * 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489537A (en) * 1991-12-13 1996-02-06 Bainbridge Sciences, Inc. Agglutination assays and kits employing colloidal dyes
USRE36054E (en) * 1992-12-07 1999-01-19 Xylum Corporation Disposable cartridge for investigating physical properties of blood
US5798215A (en) * 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
US5491095A (en) * 1993-05-26 1996-02-13 Westinghouse Electric Corp. System and method for aggulutination detection
US5939021A (en) * 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay

Also Published As

Publication number Publication date
AU2001275985A1 (en) 2002-03-26
WO2002023154A2 (en) 2002-03-21

Similar Documents

Publication Publication Date Title
WO2002023154A3 (en) Aggregation-based assays
WO2005026681A3 (en) Magnetostrictive ligand sensor devices and uses thereof
AU2805697A (en) Analyte assay using particulate labels
WO2005106036A3 (en) Methods and compositions for assaying analytes
WO2006085958A3 (en) Integration of direct binding sensors with mass spectrometry for functional and structural characterization of molecules
SG60022A1 (en) Electrochemicalmiliuminescent assays
EP2248912B8 (en) High sensitivity assays for pathogen detection using metal-enhanced fluorescence
WO2004041061A3 (en) Substrates, devices, and methods for cellular assays
AU2002366904A1 (en) Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array
WO2002079490A3 (en) Bio-barcodes based on oligonucleotide-modified particles
WO2001059161A3 (en) Analyte assays employing universal arrays
HK1080142A1 (en) Sensitive immunochromatographic assay
DE69028639D1 (en) ENZYME-ENHANCED LANTHANIDE CHELATE LUMINESCENCE DETECTION
WO2001050129A3 (en) Compensation for non-specific signals in quantitative immunoassays
WO2006109311A3 (en) Enzyme-channeling based electrochemical biosensors
DE60042370D1 (en) DETECTION OF NUCLEIC ACIDS
EP1710583A3 (en) Confirmatory dipstick assay
WO2005042771A3 (en) Colorimetric substrates, colorimetric sensors, and methods of use
GB0719967D0 (en) Liquid flow assays utilising a combined detection and control zone
WO2005079420A3 (en) Chromatographic exclusion agglutination assay and methods of use thereof
ES2106121T3 (en) DIANA COMPONENT TEST.
TWI266052B (en) Fluorescent intensity method for assaying binding between proteins or peptides
EP1249503A3 (en) Multiplexed gene analysis on a mobile solid support
EP0677589A3 (en) Method, test element and test kit for semi-quantitative detection of target nucleic acid
AU2001266902A1 (en) High throughput screening of compounds for biological activity

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP