WO2002020838A3 - Method and system for detecting nucleic acids - Google Patents

Method and system for detecting nucleic acids Download PDF

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Publication number
WO2002020838A3
WO2002020838A3 PCT/IL2001/000808 IL0100808W WO0220838A3 WO 2002020838 A3 WO2002020838 A3 WO 2002020838A3 IL 0100808 W IL0100808 W IL 0100808W WO 0220838 A3 WO0220838 A3 WO 0220838A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
nucleic acids
sample
detecting
Prior art date
Application number
PCT/IL2001/000808
Other languages
French (fr)
Other versions
WO2002020838A2 (en
Inventor
Fernando Patolsky
Amir Lichtenstein
Itamar Willner
Yossi Weizmann
Original Assignee
Yissum Res Dev Co
Fernando Patolsky
Amir Lichtenstein
Itamar Willner
Yossi Weizmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co, Fernando Patolsky, Amir Lichtenstein, Itamar Willner, Yossi Weizmann filed Critical Yissum Res Dev Co
Priority to US10/363,644 priority Critical patent/US20040023258A1/en
Priority to EP01961113A priority patent/EP1379684A2/en
Priority to AU2001282491A priority patent/AU2001282491A1/en
Priority to MXPA03001879A priority patent/MXPA03001879A/en
Priority to JP2002525844A priority patent/JP2004526409A/en
Priority to CA002421204A priority patent/CA2421204A1/en
Priority to NZ524956A priority patent/NZ524956A/en
Publication of WO2002020838A2 publication Critical patent/WO2002020838A2/en
Publication of WO2002020838A3 publication Critical patent/WO2002020838A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00713—Electrochemical synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method for the detection of a target nucleic acid in a sample containing a mixture of nucleic acids. The method comprises (a) providing a solid surface; (b) attaching to the solid surface an oligonucleotide probe complementary to a segment of the target nucleic acid; (c) contacting the surface with the sample, thereby allowing the probe to bind the target nucleic acid; (d) incubating the bound target nucleic acid with the 4 nucleotide types and a replication biocatalyst thereby forming a multi-stranded nucleic acid assembly, wherein at least one of the nucleotide types is bound by a label; and (e) detecting the label on the multi-stranded nucleic acid assembly, thereby detecting the target nucleic acid. Also disclosed are a system for identifying a target nucleic acid sequence in a sample and a kit for use in the method.
PCT/IL2001/000808 2000-09-04 2001-08-29 Method and system for detecting nucleic acids WO2002020838A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US10/363,644 US20040023258A1 (en) 2000-09-04 2001-08-29 Method and system or detecting nucleic acids
EP01961113A EP1379684A2 (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids
AU2001282491A AU2001282491A1 (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids
MXPA03001879A MXPA03001879A (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids.
JP2002525844A JP2004526409A (en) 2000-09-04 2001-08-29 Methods and systems for detecting nucleic acids
CA002421204A CA2421204A1 (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids
NZ524956A NZ524956A (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL13822900A IL138229A (en) 2000-09-04 2000-09-04 Method and system for detecting nucleic acids
IL138229 2000-09-04

Publications (2)

Publication Number Publication Date
WO2002020838A2 WO2002020838A2 (en) 2002-03-14
WO2002020838A3 true WO2002020838A3 (en) 2003-10-23

Family

ID=11074586

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/000808 WO2002020838A2 (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids

Country Status (9)

Country Link
US (1) US20040023258A1 (en)
EP (1) EP1379684A2 (en)
JP (1) JP2004526409A (en)
AU (1) AU2001282491A1 (en)
CA (1) CA2421204A1 (en)
IL (1) IL138229A (en)
MX (1) MXPA03001879A (en)
NZ (1) NZ524956A (en)
WO (1) WO2002020838A2 (en)

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DE10221885B4 (en) * 2002-05-16 2005-12-29 Infineon Technologies Ag Sensor unit, sensor arrangement and method for operating a sensor unit
DE10311315A1 (en) * 2003-03-14 2004-09-30 Apibio Sas Method and device for the detection of biomolecules
AU2004236714B2 (en) * 2003-05-02 2009-11-19 Geneohm Sciences, Inc. Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen
US7642064B2 (en) * 2003-06-24 2010-01-05 Ventana Medical Systems, Inc. Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
US20050186590A1 (en) * 2003-11-10 2005-08-25 Crothers Donald M. Nucleic acid detection method having increased sensitivity
JP2006182757A (en) * 2004-10-06 2006-07-13 Japan Science & Technology Agency METHOD OF DETECTING COMPLEMENTARY STRAND NUCLEIC ACID MOLECULE SEQUENCE AND METHOD OF DETECTING SNP USING pi-CONJUGATION TYPE ELECTROCHEMICALLY ACTIVE NONNATURAL NUCLEOSIDE
DE102005037436A1 (en) * 2005-08-04 2007-02-15 Siemens Ag Method and system for determining the concentration of an analyte-enzyme complex or analyte-enzyme conjugate, in particular for the electrochemical detection of the analyte, and associated measuring device
US20070231794A1 (en) * 2005-09-21 2007-10-04 Combimatrix Corporation Process to detect binding events on an electrode microarray using enzymes
US8855955B2 (en) * 2005-09-29 2014-10-07 Custom Array, Inc. Process and apparatus for measuring binding events on a microarray of electrodes
US7820391B2 (en) 2007-11-06 2010-10-26 Osmetech Molecular Diagnostics Baseless nucleotide analogues and uses thereof
CN101241125B (en) * 2008-03-10 2013-02-13 施晓燕 Piezoelectric sensor for liquid phase measuring and encapsulation method
US20100069253A1 (en) * 2008-09-12 2010-03-18 Gindilis Andrei L Impedance Spectroscopy Measurement of DNA
WO2011102065A1 (en) * 2010-02-17 2011-08-25 株式会社 村田製作所 Method of detection using mass detection sensor
GB201207585D0 (en) 2012-05-01 2012-06-13 Isis Innovation An electrode and use thereof
JP2014006208A (en) * 2012-06-27 2014-01-16 Nippon Dempa Kogyo Co Ltd Sensing method
US9416390B2 (en) * 2012-07-27 2016-08-16 Ohmx Corporation Electric measurement of monolayers following pro-cleave detection of presence and activity of enzymes and other target analytes
EP2877592B1 (en) 2012-07-27 2016-09-21 Ohmx Corporation Electronic measurements of monolayers following homogeneous reactions of their components
CN105593379A (en) * 2013-08-07 2016-05-18 艾克泽基因公司 Systems, methods and devices for electrochemical detection using helper oligonucleotides
ES2762743T3 (en) 2013-12-12 2020-05-25 Hollister Inc Downloadable catheters
EP3079748B1 (en) 2013-12-12 2020-05-27 Hollister Incorporated Flushable disintegration catheter
ES2802950T3 (en) 2013-12-12 2021-01-22 Hollister Inc Disposable toilet catheters
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CN105866226A (en) * 2016-06-07 2016-08-17 辽宁科技大学 Preparation and using method of glucose oxidase biosensor
JP6346242B2 (en) * 2016-11-04 2018-06-20 株式会社東芝 Nucleic acid detection method and assay kit
CN112326763B (en) * 2020-09-24 2022-10-21 福建医科大学附属第一医院 DNA nano-sieve reproducible electrochemical sensor for clinical sample allelic genotyping

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US5871906A (en) * 1991-11-08 1999-02-16 The Research Foundation Of State University Of New York Method for detection of amplified nucleic acid products and related diagnostic assays
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Also Published As

Publication number Publication date
WO2002020838A2 (en) 2002-03-14
AU2001282491A1 (en) 2002-03-22
EP1379684A2 (en) 2004-01-14
JP2004526409A (en) 2004-09-02
MXPA03001879A (en) 2004-04-20
IL138229A (en) 2005-03-20
US20040023258A1 (en) 2004-02-05
IL138229A0 (en) 2001-10-31
NZ524956A (en) 2005-01-28
CA2421204A1 (en) 2002-03-14

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