WO2002012566A3 - The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix - Google Patents

The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix Download PDF

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Publication number
WO2002012566A3
WO2002012566A3 PCT/US2001/024894 US0124894W WO0212566A3 WO 2002012566 A3 WO2002012566 A3 WO 2002012566A3 US 0124894 W US0124894 W US 0124894W WO 0212566 A3 WO0212566 A3 WO 0212566A3
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WO
WIPO (PCT)
Prior art keywords
photocycloaddition
immobilization
oligonucleotides
evaluation
hydrogel matrix
Prior art date
Application number
PCT/US2001/024894
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French (fr)
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WO2002012566A2 (en
Inventor
Robert Elghanian
Charles K Brush
Yanzheng Xu
Original Assignee
Motorola Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Motorola Inc filed Critical Motorola Inc
Priority to EP01957507A priority Critical patent/EP1307414A2/en
Priority to AU2001279244A priority patent/AU2001279244A1/en
Publication of WO2002012566A2 publication Critical patent/WO2002012566A2/en
Publication of WO2002012566A3 publication Critical patent/WO2002012566A3/en

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

[0064] [2+2]photo-attachable functional groups were incorporated in polyacrylamide based hydrogels and synthetic oligonucleotide probes. The probes were photochemically attached by covalent bonding to the three dimensional surface of a hydrogel. The resultant hydrogel microarrays were used for detection of specific target oligonulcleotides, including mRNA and DNA, as suitable for expression and single nucleotide polymorphism type microarray analyses.
PCT/US2001/024894 2000-08-09 2001-08-09 The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix WO2002012566A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01957507A EP1307414A2 (en) 2000-08-09 2001-08-09 The use and evaluation of a 2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
AU2001279244A AU2001279244A1 (en) 2000-08-09 2001-08-09 The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22407000P 2000-08-09 2000-08-09
US60/224,070 2000-08-09
US23230500P 2000-09-12 2000-09-12
US60/232,305 2000-09-12

Publications (2)

Publication Number Publication Date
WO2002012566A2 WO2002012566A2 (en) 2002-02-14
WO2002012566A3 true WO2002012566A3 (en) 2003-01-09

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Family Applications (1)

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PCT/US2001/024894 WO2002012566A2 (en) 2000-08-09 2001-08-09 The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix

Country Status (3)

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EP (1) EP1307414A2 (en)
AU (1) AU2001279244A1 (en)
WO (1) WO2002012566A2 (en)

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EP1307414A2 (en) 2003-05-07
WO2002012566A2 (en) 2002-02-14
AU2001279244A1 (en) 2002-02-18

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