WO2002012566A3 - The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix - Google Patents

The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix Download PDF

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Publication number
WO2002012566A3
WO2002012566A3 PCT/US2001/024894 US0124894W WO0212566A3 WO 2002012566 A3 WO2002012566 A3 WO 2002012566A3 US 0124894 W US0124894 W US 0124894W WO 0212566 A3 WO0212566 A3 WO 0212566A3
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WIPO (PCT)
Prior art keywords
photocycloaddition
immobilization
oligonucleotides
evaluation
hydrogel matrix
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Application number
PCT/US2001/024894
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French (fr)
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WO2002012566A2 (en
Inventor
Robert Elghanian
Charles K Brush
Yanzheng Xu
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Motorola Inc
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Publication date
Application filed by Motorola Inc filed Critical Motorola Inc
Priority to AU2001279244A priority Critical patent/AU2001279244A1/en
Priority to EP01957507A priority patent/EP1307414A2/en
Publication of WO2002012566A2 publication Critical patent/WO2002012566A2/en
Publication of WO2002012566A3 publication Critical patent/WO2002012566A3/en

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

[0064] [2+2]photo-attachable functional groups were incorporated in polyacrylamide based hydrogels and synthetic oligonucleotide probes. The probes were photochemically attached by covalent bonding to the three dimensional surface of a hydrogel. The resultant hydrogel microarrays were used for detection of specific target oligonulcleotides, including mRNA and DNA, as suitable for expression and single nucleotide polymorphism type microarray analyses.
PCT/US2001/024894 2000-08-09 2001-08-09 The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix WO2002012566A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001279244A AU2001279244A1 (en) 2000-08-09 2001-08-09 The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
EP01957507A EP1307414A2 (en) 2000-08-09 2001-08-09 The use and evaluation of a 2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22407000P 2000-08-09 2000-08-09
US60/224,070 2000-08-09
US23230500P 2000-09-12 2000-09-12
US60/232,305 2000-09-12

Publications (2)

Publication Number Publication Date
WO2002012566A2 WO2002012566A2 (en) 2002-02-14
WO2002012566A3 true WO2002012566A3 (en) 2003-01-09

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PCT/US2001/024894 WO2002012566A2 (en) 2000-08-09 2001-08-09 The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix

Country Status (3)

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EP (1) EP1307414A2 (en)
AU (1) AU2001279244A1 (en)
WO (1) WO2002012566A2 (en)

Cited By (1)

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Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6921638B2 (en) 1999-06-25 2005-07-26 Amersham Biosciences Ab Hydrogel-based microarray signal amplification methods and devices therefor
US6664061B2 (en) * 1999-06-25 2003-12-16 Amersham Biosciences Ab Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
EP3673986A1 (en) 2004-01-07 2020-07-01 Illumina Cambridge Limited Improvements in or relating to molecular arrays
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
CN105263918B (en) 2013-03-08 2018-05-18 伊鲁米纳剑桥有限公司 Rhodamine compound and its purposes as fluorescent marker
WO2014135221A1 (en) 2013-03-08 2014-09-12 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
ES2899618T3 (en) 2013-07-01 2022-03-14 Illumina Inc Catalyst-free surface functionalization and polymer grafting
CN105874385B (en) 2013-12-19 2021-08-20 Illumina公司 Substrate comprising a nanopatterned surface and method for the production thereof
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
US20180207920A1 (en) 2015-07-17 2018-07-26 Illumina, Inc. Polymer sheets for sequencing applications
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
EP4257596A3 (en) 2016-12-22 2023-12-06 Illumina Cambridge Limited Coumarin compounds and their uses as fluorescent labels
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
US11390753B2 (en) 2019-03-01 2022-07-19 Illumina Cambridge Limited Exocyclic amine substituted coumarin compounds and uses as fluorescent labels
JP2022521866A (en) 2019-03-01 2022-04-13 イルミナ ケンブリッジ リミテッド Tertiary amine substituted coumarin compounds and their use as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
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BR112022026056A2 (en) 2020-06-22 2023-03-07 Illumina Cambridge Ltd NUCLEOSIDES AND NUCLEOTIDES WITH BLOCKING GROUP 3? ACETAL
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
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CA3216735A1 (en) 2021-05-20 2022-11-24 Patrizia IAVICOLI Compositions and methods for sequencing by synthesis
AU2022413575A1 (en) 2021-12-16 2024-01-18 Illumina, Inc. Methods for metal directed cleavage of surface-bound polynucleotides
WO2023122499A1 (en) 2021-12-20 2023-06-29 Illumina Cambridge Limited Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
AU2022419500A1 (en) 2021-12-20 2024-01-18 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
CA3223115A1 (en) 2022-03-28 2023-10-05 Xiaolin Wu Labeled avidin and methods for sequencing
WO2023186819A1 (en) 2022-03-29 2023-10-05 Illumina Cambridge Limited Chromenoquinoline dyes and uses in sequencing
CA3223037A1 (en) 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
AU2023244351A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
WO2023232829A1 (en) 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing
WO2024003087A1 (en) 2022-06-28 2024-01-04 Illumina, Inc. Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024068889A2 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing
WO2024123866A1 (en) 2022-12-09 2024-06-13 Illumina, Inc. Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015798A1 (en) * 1989-06-19 1990-12-27 Boehringer Mannheim Gmbh Aminoalkyl maleimides and haptene and antigen derivatives thereof and conjugates with peptides or proteins
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces
WO1998030575A1 (en) * 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
WO2000034457A1 (en) * 1998-12-10 2000-06-15 Takara Shuzo Co., Ltd. Method for immobilizing oligonucleotide on a carrier
WO2001001143A2 (en) * 1999-06-25 2001-01-04 Motorola Inc. Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker
WO2001084234A1 (en) * 2000-05-01 2001-11-08 Proligo Llc Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015798A1 (en) * 1989-06-19 1990-12-27 Boehringer Mannheim Gmbh Aminoalkyl maleimides and haptene and antigen derivatives thereof and conjugates with peptides or proteins
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces
WO1998030575A1 (en) * 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
WO2000034457A1 (en) * 1998-12-10 2000-06-15 Takara Shuzo Co., Ltd. Method for immobilizing oligonucleotide on a carrier
WO2001001143A2 (en) * 1999-06-25 2001-01-04 Motorola Inc. Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker
WO2001084234A1 (en) * 2000-05-01 2001-11-08 Proligo Llc Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 200036, Derwent World Patents Index; Class B04, AN 2000-423411, XP002203841 *
ELSNER H I ET AL: "USE OF PSORALENS FOR COVALENT IMMOBILIZATION OF BIOMOLECULES IN SOLID PHASE ASSAYS", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 5, no. 5, 1 September 1994 (1994-09-01), pages 463 - 467, XP000465957, ISSN: 1043-1802 *
M. N. YOUSAF, M. MRKSICH: "Diels-Alder reaction for the selective immobilization of protein to electroactive self-assembled monolayers", J. AM. CHEM. SOC., vol. 121, 1999, pages 4286 - 4287, XP002203840 *
REHMAN F ET AL: "Immobilization of acrylamide-modified oligonucleotides by co-polymerization", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 2, 15 February 1999 (1999-02-15), pages 649 - 655, XP002155808, ISSN: 0305-1048 *
YI-ZHONG A ET AL: "Sequence-Specific Modification of Guanosine in DNA by a C60-Linked Deoxyoligonucleotide: Evidence for a Non-Singlet Oxygen Mechanism", TETRAHEDRON, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 52, no. 14, 1 April 1996 (1996-04-01), pages 5179 - 5189, XP004104270, ISSN: 0040-4020 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12031178B2 (en) 2023-02-14 2024-07-09 Illumina Cambridge Limited Cyclooctatetraene containing dyes and compositions

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AU2001279244A1 (en) 2002-02-18
EP1307414A2 (en) 2003-05-07

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