WO2002012555A3 - Means for targeting nucleic acid repeat regions - Google Patents

Means for targeting nucleic acid repeat regions Download PDF

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Publication number
WO2002012555A3
WO2002012555A3 PCT/FR2001/002484 FR0102484W WO0212555A3 WO 2002012555 A3 WO2002012555 A3 WO 2002012555A3 FR 0102484 W FR0102484 W FR 0102484W WO 0212555 A3 WO0212555 A3 WO 0212555A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide
nucleic acid
family
acid repeat
nucleotides
Prior art date
Application number
PCT/FR2001/002484
Other languages
French (fr)
Other versions
WO2002012555A2 (en
Inventor
Pierre Commercon
Original Assignee
Dosagene R & D
Pierre Commercon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dosagene R & D, Pierre Commercon filed Critical Dosagene R & D
Priority to AU2001279929A priority Critical patent/AU2001279929A1/en
Publication of WO2002012555A2 publication Critical patent/WO2002012555A2/en
Publication of WO2002012555A3 publication Critical patent/WO2002012555A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention concerns a method for preparing nucleotide fragments specific of a family of nucleic acid repeat regions, or target family, said regions having among them high homologies. The invention is characterised in that it comprises steps which consist in: aligning, one beneath the other, at least 10 monomer sequences of the target family of at least 20 nucleotides in length; defining on each of the monomer sequences, one or several matching zones, on each of the monomers; evaluating the occurrence percentage, in each vertical alignment, of each of the nucleotides of the selected zone; statistically producing nucleotide fragments, in accordance with a probabilistic fuzzy logic, by selecting for each position, based on the percentage previously established, and by comparing with the percentages obtained, by the same process, with other nucleic acids of different families, either the most probable nucleotide, so as to provide the fragment with a polymorphous character, or the nucleotide most specific with respect to the target family.
PCT/FR2001/002484 2000-08-03 2001-07-27 Means for targeting nucleic acid repeat regions WO2002012555A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001279929A AU2001279929A1 (en) 2000-08-03 2001-07-27 Means for targeting nucleic acid repeat regions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0010279A FR2812641A1 (en) 2000-08-03 2000-08-03 MEANS FOR TARGETING REPEATED NUCLEIC ACID REGIONS
FR00/10279 2000-08-03

Publications (2)

Publication Number Publication Date
WO2002012555A2 WO2002012555A2 (en) 2002-02-14
WO2002012555A3 true WO2002012555A3 (en) 2003-10-30

Family

ID=8853284

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2001/002484 WO2002012555A2 (en) 2000-08-03 2001-07-27 Means for targeting nucleic acid repeat regions

Country Status (3)

Country Link
AU (1) AU2001279929A1 (en)
FR (1) FR2812641A1 (en)
WO (1) WO2002012555A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2357240A1 (en) * 2003-06-27 2011-08-17 Chromatin, Inc. Plant centromere compositions
US9096909B2 (en) 2009-07-23 2015-08-04 Chromatin, Inc. Sorghum centromere sequences and minichromosomes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007092A1 (en) * 1990-10-18 1992-04-30 Genmap, Inc. Method of obtaining a physical map of a genome
EP0511750A1 (en) * 1991-04-09 1992-11-04 The Regents Of The University Of California Repeat sequence chromosome specific nucleic acid probes
WO1996000234A1 (en) * 1994-06-23 1996-01-04 Aprogenex, Inc. Centromere hybridization probes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007092A1 (en) * 1990-10-18 1992-04-30 Genmap, Inc. Method of obtaining a physical map of a genome
EP0511750A1 (en) * 1991-04-09 1992-11-04 The Regents Of The University Of California Repeat sequence chromosome specific nucleic acid probes
WO1996000234A1 (en) * 1994-06-23 1996-01-04 Aprogenex, Inc. Centromere hybridization probes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JORGENSEN A L ET AL: "HOMOLOGOUS SUBFAMILIES OF HUMAN ALPHOID REPETITIVE DNA ON DIFFERENT NUCLEOLUS ORGANIZING CHROMOSOMES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 84, no. 4, 1987, 1987, pages 1075 - 1079, XP002165482, ISSN: 0027-8424 *
MARATOU KLIO ET AL: "Novel methodology for the detection of chromosome 21-specific alpha-satellite DNA sequences.", GENOMICS, vol. 57, no. 3, 1 May 1999 (1999-05-01), pages 429 - 432, XP002165481, ISSN: 0888-7543 *
PELLESTOR F ET AL: "FISH AND PRINS, A STRATEGY FOR RAPID CHROMOSOME SCREENING: APPLICATION TO THE ASSESSMENT OF ANEUPLOIDY IN HUMAN SPERM", CYTOGENETICS AND CELL GENETICS, BASEL, CH, vol. 72, 1996, pages 34 - 36, XP008006429, ISSN: 0301-0171 *
WARBURTON P E ET AL: "PCR AMPLIFICATION OF CHROMOSOME-SPECIFIC ALPHA SATELLITE DNA DEFINITION OF CENTROMERIC STS MARKERS AND POLYMORPHIC ANALYSIS", GENOMICS, vol. 11, no. 2, 1991, pages 324 - 333, XP000992859, ISSN: 0888-7543 *
WEIER H -U G ET AL: "LABELING OF THE CENTROMERIC REGION ON HUMAN CHROMOSOME 8 BY IN SITUHYBRIDIZATION", HUMAN GENETICS,DE,BERLIN, vol. 87, 1991, pages 489 - 494, XP000283203 *

Also Published As

Publication number Publication date
WO2002012555A2 (en) 2002-02-14
FR2812641A1 (en) 2002-02-08
AU2001279929A1 (en) 2002-02-18

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