WO2002008965A3 - Method for determining spatial distances in polymers - Google Patents
Method for determining spatial distances in polymers Download PDFInfo
- Publication number
- WO2002008965A3 WO2002008965A3 PCT/EP2001/008628 EP0108628W WO0208965A3 WO 2002008965 A3 WO2002008965 A3 WO 2002008965A3 EP 0108628 W EP0108628 W EP 0108628W WO 0208965 A3 WO0208965 A3 WO 0208965A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cross
- polymer
- complexes
- fragments
- polymer molecules
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
Abstract
The invention relates to a method for determining spatial distances in polymer molecules of a known monomer sequence or complexes of such polymer molecules, whereby a) the polymer molecules or complexes are transformed by means of mixtures of homo or hetero-bifunctional cross-linker molecules of varying length and mass and possibly comprising varying chromophores, but having the same binding properties as the reactive terminal groups, b) after reacting with the mixture of cross-links, the polymer molecules or complexes are specifically split at known splitting positions of the polymer sequences, c) the resulting fragments are measured, the mass thereof being preferably determined, d) the resulting fragments are identified especially the fragments in which the cross-linkers connect two polymer or complex residues by comparing the measurement results from step c) with the expected values of theoretical fragments, said values being determined on the basis of known data on monomer sequences, splitting positions, cross-linkers etc., e) naturally available linkages (for example disulfide bridges in proteins) are taken into account, f) the cross-linker having the shortest length of all the cross-linkers of the mixture links the two residues, said length being a measurement for the distance between the two residues.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10036342.3 | 2000-07-26 | ||
| DE2000136342 DE10036342C2 (en) | 2000-07-26 | 2000-07-26 | Method for determining spatial distances in polymers or complexes of polymers using mixtures of cross-linker molecules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2002008965A2 WO2002008965A2 (en) | 2002-01-31 |
| WO2002008965A3 true WO2002008965A3 (en) | 2003-04-10 |
Family
ID=7650249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2001/008628 WO2002008965A2 (en) | 2000-07-26 | 2001-07-26 | Method for determining spatial distances in polymers |
Country Status (2)
| Country | Link |
|---|---|
| DE (1) | DE10036342C2 (en) |
| WO (1) | WO2002008965A2 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993024834A1 (en) * | 1992-05-29 | 1993-12-09 | The Rockefeller University | Method and product for the sequence determination of peptides using a mass spectrometer |
| US5453937A (en) * | 1993-04-28 | 1995-09-26 | Immunex Corporation | Method and system for protein modeling |
| WO1998048270A1 (en) * | 1997-04-22 | 1998-10-29 | California Institute Of Technology | Method of determining three-dimensional protein structure from primary protein sequence |
| WO1999041607A2 (en) * | 1998-02-14 | 1999-08-19 | Gmd Forschungszentrum Informationstechnik Gmbh | Fluorescent energy for elucidating the 3-d structure of biological macromolecules |
-
2000
- 2000-07-26 DE DE2000136342 patent/DE10036342C2/en not_active Expired - Lifetime
-
2001
- 2001-07-26 WO PCT/EP2001/008628 patent/WO2002008965A2/en active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993024834A1 (en) * | 1992-05-29 | 1993-12-09 | The Rockefeller University | Method and product for the sequence determination of peptides using a mass spectrometer |
| US5453937A (en) * | 1993-04-28 | 1995-09-26 | Immunex Corporation | Method and system for protein modeling |
| WO1998048270A1 (en) * | 1997-04-22 | 1998-10-29 | California Institute Of Technology | Method of determining three-dimensional protein structure from primary protein sequence |
| WO1999041607A2 (en) * | 1998-02-14 | 1999-08-19 | Gmd Forschungszentrum Informationstechnik Gmbh | Fluorescent energy for elucidating the 3-d structure of biological macromolecules |
Also Published As
| Publication number | Publication date |
|---|---|
| DE10036342A1 (en) | 2002-03-07 |
| DE10036342C2 (en) | 2002-09-19 |
| WO2002008965A2 (en) | 2002-01-31 |
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