WO2002008734A3 - Methods using light emission for determining the effectiveness of plant treatment agents in controlling plant disease organisms - Google Patents

Methods using light emission for determining the effectiveness of plant treatment agents in controlling plant disease organisms Download PDF

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Publication number
WO2002008734A3
WO2002008734A3 PCT/US2001/022932 US0122932W WO0208734A3 WO 2002008734 A3 WO2002008734 A3 WO 2002008734A3 US 0122932 W US0122932 W US 0122932W WO 0208734 A3 WO0208734 A3 WO 0208734A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
effectiveness
determining
substrate
plant
Prior art date
Application number
PCT/US2001/022932
Other languages
French (fr)
Other versions
WO2002008734A2 (en
Inventor
Shawn Louise Anderson
Kathleen Ann Lu
Original Assignee
Du Pont
Shawn Louise Anderson
Kathleen Ann Lu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Shawn Louise Anderson, Kathleen Ann Lu filed Critical Du Pont
Priority to US10/343,073 priority Critical patent/US20040033555A1/en
Priority to EP01959077A priority patent/EP1303749A2/en
Priority to JP2002514377A priority patent/JP2004506879A/en
Publication of WO2002008734A2 publication Critical patent/WO2002008734A2/en
Publication of WO2002008734A3 publication Critical patent/WO2002008734A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Methods are provided for determining the effectiveness of a plant treatment agent in controlling a plant disease organism. The methods involve (a) applying the plant treatment agent to a substrate, (b) inoculating the substrate with the disease organism, and detecting photon emission from light-generating moieties. The methods also involve (1c) after a period of time in which the disease organism can grow, applying a pro-light-generating moiety which is selectively transformed by the disease organism or the substrate to a light-generating moiety, (1d) detecting photon emission from the light-generating moiety (e.g. over an area of the substrate), and (1e) determining the effectiveness of the plant treatment agent based on the amount of detected photon emission and/or the fraction of the substrate area from which photon emission is detected.
PCT/US2001/022932 2000-07-26 2001-07-20 Methods using light emission for determining the effectiveness of plant treatment agents in controlling plant disease organisms WO2002008734A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/343,073 US20040033555A1 (en) 2001-07-20 2001-07-20 Methods using light emission for determining the effeciveness of plant treatment agents in controlling plant disease organisms
EP01959077A EP1303749A2 (en) 2000-07-26 2001-07-20 Methods using light emission for determining the effectiveness of plant treatment agents in controlling plant disease organisms
JP2002514377A JP2004506879A (en) 2000-07-26 2001-07-20 Method of determining the effectiveness of a plant treatment in controlling plant disease organisms using light radiation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22093300P 2000-07-26 2000-07-26
US60/220,933 2000-07-26

Publications (2)

Publication Number Publication Date
WO2002008734A2 WO2002008734A2 (en) 2002-01-31
WO2002008734A3 true WO2002008734A3 (en) 2002-08-01

Family

ID=22825617

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/022932 WO2002008734A2 (en) 2000-07-26 2001-07-20 Methods using light emission for determining the effectiveness of plant treatment agents in controlling plant disease organisms

Country Status (3)

Country Link
EP (1) EP1303749A2 (en)
JP (1) JP2004506879A (en)
WO (1) WO2002008734A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1590656A1 (en) * 2003-01-24 2005-11-02 Plant Research International B.V. Identification labels in plants or plant parts
JP5124216B2 (en) * 2007-09-14 2013-01-23 オリンパス株式会社 Optical signal observation method and optical signal observation system
EP2056094A1 (en) * 2007-10-12 2009-05-06 Universiteit Hasselt Fluorescence imaging system

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5474909A (en) * 1992-08-07 1995-12-12 Anticancer, Inc. Noncolorimetric histoculture method for predicting drug response of tumors
US5968762A (en) * 1998-03-19 1999-10-19 The University Of Connecticut Method for detecting bacteria in a sample

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5474909A (en) * 1992-08-07 1995-12-12 Anticancer, Inc. Noncolorimetric histoculture method for predicting drug response of tumors
US5968762A (en) * 1998-03-19 1999-10-19 The University Of Connecticut Method for detecting bacteria in a sample

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SPELLIG T ET AL: "GREEN FLUORESCENT PROTEIN (GFP) AS A NEW VITAL MARKER IN THE PHYTOPATHOGENIC FUNGUS USTILAGO MAYDIS", MOLECULAR AND GENERAL GENETICS, SPRINGER VERLAG, BERLIN, DE, vol. 252, no. 5, 1996, pages 503 - 509, XP001024124, ISSN: 0026-8925 *

Also Published As

Publication number Publication date
EP1303749A2 (en) 2003-04-23
JP2004506879A (en) 2004-03-04
WO2002008734A2 (en) 2002-01-31

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