WO2002008722A3 - Direct assessment of relative concentrations of variants of an epitope on a dimeric molecule - Google Patents

Direct assessment of relative concentrations of variants of an epitope on a dimeric molecule Download PDF

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Publication number
WO2002008722A3
WO2002008722A3 PCT/EP2001/007985 EP0107985W WO0208722A3 WO 2002008722 A3 WO2002008722 A3 WO 2002008722A3 EP 0107985 W EP0107985 W EP 0107985W WO 0208722 A3 WO0208722 A3 WO 0208722A3
Authority
WO
WIPO (PCT)
Prior art keywords
variants
epitope
relative concentrations
analyte
direct assessment
Prior art date
Application number
PCT/EP2001/007985
Other languages
French (fr)
Other versions
WO2002008722A2 (en
Inventor
Werner Naser
Original Assignee
Werner Naser
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Werner Naser filed Critical Werner Naser
Priority to AU2001276395A priority Critical patent/AU2001276395A1/en
Publication of WO2002008722A2 publication Critical patent/WO2002008722A2/en
Publication of WO2002008722A3 publication Critical patent/WO2002008722A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/10—Musculoskeletal or connective tissue disorders
    • G01N2800/108—Osteoporosis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to methods and devices for the assessment of the ratio of variants of an epitope on an analyte molecule, comprising at least two variants of said epitope, by aid of a first and a second specific binding partner for said epitope variants. The result of measurements according to the present invention is directly or indirectly correlated to the ratio of variants of an epitope carried by the analyte under investigation. The methods and devices described are applied to rather variable samples and yield results essentially independent of variations in the absolute values of the analyte investigated.
PCT/EP2001/007985 2000-07-12 2001-07-11 Direct assessment of relative concentrations of variants of an epitope on a dimeric molecule WO2002008722A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001276395A AU2001276395A1 (en) 2000-07-12 2001-07-11 Direct assessment of relative concentrations of variants of an epitope on a dimeric molecule

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00115163.8 2000-07-12
EP00115163 2000-07-12

Publications (2)

Publication Number Publication Date
WO2002008722A2 WO2002008722A2 (en) 2002-01-31
WO2002008722A3 true WO2002008722A3 (en) 2002-05-16

Family

ID=8169239

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/007985 WO2002008722A2 (en) 2000-07-12 2001-07-11 Direct assessment of relative concentrations of variants of an epitope on a dimeric molecule

Country Status (2)

Country Link
AU (1) AU2001276395A1 (en)
WO (1) WO2002008722A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020764A1 (en) * 1994-01-31 1995-08-03 Boehringer Mannheim Gmbh Specific binding substances for antibodies and their use for immunoassays or vaccines
WO1997008551A1 (en) * 1995-08-31 1997-03-06 First Medical, Inc. Methods and antibodies for detecting creatine kinase
WO1998026286A2 (en) * 1996-12-09 1998-06-18 Osteometer Biotech A/S Sandwich assays for collagen type i fragments
WO1998052041A1 (en) * 1997-05-14 1998-11-19 Biosite Diagnostics Incorporated Rapid evaluation of the ratio of biological molecules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020764A1 (en) * 1994-01-31 1995-08-03 Boehringer Mannheim Gmbh Specific binding substances for antibodies and their use for immunoassays or vaccines
WO1997008551A1 (en) * 1995-08-31 1997-03-06 First Medical, Inc. Methods and antibodies for detecting creatine kinase
WO1998026286A2 (en) * 1996-12-09 1998-06-18 Osteometer Biotech A/S Sandwich assays for collagen type i fragments
WO1998052041A1 (en) * 1997-05-14 1998-11-19 Biosite Diagnostics Incorporated Rapid evaluation of the ratio of biological molecules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
C. FLEDELIUS ET AL.: "Characterization of urinary degradation products derived from type I collagen", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 272, no. 15, 11 April 1997 (1997-04-11), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 9755 - 9763, XP002157131, ISSN: 0021-9258 *
J. M. BLATT ET AL.: "A miniaurized, self-contained, single-use, disposable assay device for the quantitative determination of the bone resorption marker NTx, in urine", CLINICAL CHEMISTRY, vol. 44, no. 9, September 1998 (1998-09-01), WINSTON US, pages 2051 - 2052, XP002157132 *

Also Published As

Publication number Publication date
WO2002008722A2 (en) 2002-01-31
AU2001276395A1 (en) 2002-02-05

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