WO2002004956A3 - Method of identifying conformation-sensitive binding peptides and uses thereof - Google Patents

Method of identifying conformation-sensitive binding peptides and uses thereof Download PDF

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Publication number
WO2002004956A3
WO2002004956A3 PCT/US2001/021867 US0121867W WO0204956A3 WO 2002004956 A3 WO2002004956 A3 WO 2002004956A3 US 0121867 W US0121867 W US 0121867W WO 0204956 A3 WO0204956 A3 WO 0204956A3
Authority
WO
WIPO (PCT)
Prior art keywords
receptor
peptides
screening
panel
carried out
Prior art date
Application number
PCT/US2001/021867
Other languages
French (fr)
Other versions
WO2002004956A9 (en
WO2002004956A2 (en
Inventor
Dana M Fowlkes
Thomas R Barnett
Benjamin Buehrer
Original Assignee
Karo Bio Usa Inc
Dana M Fowlkes
Thomas R Barnett
Benjamin Buehrer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karo Bio Usa Inc, Dana M Fowlkes, Thomas R Barnett, Benjamin Buehrer filed Critical Karo Bio Usa Inc
Priority to EP01957128A priority Critical patent/EP1360501A2/en
Priority to JP2002509775A priority patent/JP2004523726A/en
Priority to US10/332,708 priority patent/US20040043420A1/en
Priority to AU2001278901A priority patent/AU2001278901A1/en
Priority to CA002414572A priority patent/CA2414572A1/en
Publication of WO2002004956A2 publication Critical patent/WO2002004956A2/en
Publication of WO2002004956A9 publication Critical patent/WO2002004956A9/en
Priority to PCT/EP2003/000352 priority patent/WO2004035614A1/en
Priority to US10/346,162 priority patent/US20030224390A1/en
Publication of WO2002004956A3 publication Critical patent/WO2002004956A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04B—TRANSMISSION
    • H04B10/00—Transmission systems employing electromagnetic waves other than radio-waves, e.g. infrared, visible or ultraviolet light, or employing corpuscular radiation, e.g. quantum communication
    • H04B10/29—Repeaters
    • H04B10/291—Repeaters in which processing or amplification is carried out without conversion of the main signal from optical form
    • H04B10/2912—Repeaters in which processing or amplification is carried out without conversion of the main signal from optical form characterised by the medium used for amplification or processing
    • H04B10/2914—Repeaters in which processing or amplification is carried out without conversion of the main signal from optical form characterised by the medium used for amplification or processing using lumped semiconductor optical amplifiers [SOA]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Nanotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Signal Processing (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • Electromagnetism (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Peptides which bind a cellular (surface or intracellular) receptor, such as a nuclear receptor, may be identified by screening a combinatorial peptide library presented in the form of cells each of which coexpress one member peptide and the receptor, together with a signal producing system for reporting binding. A 'two-hybrid' assay is of particular interest. The screen may be carried out in the presence of a ligand, in particular, an exogenous ligand. If this screening is carried out for a plurality of different receptor conformations, then this library screening will also serve to identify conformation-specific peptides for the receptor, which may then be used in a panel for 'fingerprinting'query compounds as to their ability to interact with the receptor in the presence of each of the panel peptides. These fingerprints may be compared to those of reference compounds with known biological activities mediated by that receptor.
PCT/US2001/021867 2000-07-12 2001-07-11 Method of identifying conformation-sensitive binding peptides and uses thereof WO2002004956A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP01957128A EP1360501A2 (en) 2000-07-12 2001-07-11 Method of identifying conformation-sensitive binding peptides and uses thereof
JP2002509775A JP2004523726A (en) 2000-07-12 2001-07-11 Method for identifying conformationally sensitive binding peptides and uses thereof
US10/332,708 US20040043420A1 (en) 2001-07-11 2001-07-11 Method of identifying conformation-sensitive binding peptides and uses thereof
AU2001278901A AU2001278901A1 (en) 2000-07-12 2001-07-11 Method of identifying conformation-sensitive binding peptides and uses thereof
CA002414572A CA2414572A1 (en) 2000-07-12 2001-07-11 Method of identifying conformation-sensitive binding peptides and uses thereof
PCT/EP2003/000352 WO2004035614A1 (en) 2001-07-11 2003-01-15 Synthetic or partially purified peptides which can bind to specific subunits of g proteins and uses thereof
US10/346,162 US20030224390A1 (en) 2000-07-12 2003-01-17 Method of identifying conformation-sensitive binding peptides and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61486500A 2000-07-12 2000-07-12
US09/614,865 2000-07-12
US86068801A 2001-05-21 2001-05-21
US09/860,688 2001-05-21

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US86068801A Continuation-In-Part 2000-07-12 2001-05-21

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/346,162 Continuation-In-Part US20030224390A1 (en) 2000-07-12 2003-01-17 Method of identifying conformation-sensitive binding peptides and uses thereof

Publications (3)

Publication Number Publication Date
WO2002004956A2 WO2002004956A2 (en) 2002-01-17
WO2002004956A9 WO2002004956A9 (en) 2002-10-10
WO2002004956A3 true WO2002004956A3 (en) 2003-09-12

Family

ID=27087352

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021867 WO2002004956A2 (en) 2000-07-12 2001-07-11 Method of identifying conformation-sensitive binding peptides and uses thereof

Country Status (6)

Country Link
US (1) US20030224390A1 (en)
EP (1) EP1360501A2 (en)
JP (1) JP2004523726A (en)
AU (1) AU2001278901A1 (en)
CA (1) CA2414572A1 (en)
WO (1) WO2002004956A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586190B2 (en) 2000-08-18 2003-07-01 Syngenta Participations Ag Parallel high throughput method and kit
WO2004035614A1 (en) * 2001-07-11 2004-04-29 Karo Bio Ab Synthetic or partially purified peptides which can bind to specific subunits of g proteins and uses thereof
EP1353182A3 (en) * 2002-04-12 2004-02-04 Smithkline Beecham Corporation Method of predicting cell-based assay results using binding profiles
US20030235864A1 (en) * 2002-06-05 2003-12-25 Sopherion Therapeutics, Inc. Method to screen ligands using eukaryotic cell display
EP1637885A1 (en) 2004-09-16 2006-03-22 Vivalis Method of screening by using conformation sensitive peptides
JP5512077B2 (en) * 2006-11-22 2014-06-04 株式会社 資生堂 Safety evaluation method, safety evaluation system, and safety evaluation program
WO2009047762A1 (en) * 2007-10-09 2009-04-16 Yeda Research And Development Co. Ltd Compositions and peptides for treatment of envenomation by pla2 containing venoms like bungarus multicinct venom
US20100233678A1 (en) * 2007-11-13 2010-09-16 Beadling Leslie C Tunable affinity ligands for the separation and detection of target substances
AU2013203554A1 (en) * 2007-12-05 2013-05-02 Alltech Associates, Inc. Methods and apparatus for analyzing samples and collecting sample fractions
KR20140114071A (en) * 2007-12-05 2014-09-25 올테크 어소시에이츠, 인크. Method and apparatus for analyzing samples and collecting sample fractions
EP2101173A1 (en) 2008-03-14 2009-09-16 Vivalis In vitro method to determine whether a drug candidate active against a target protein is active against a variant of said protein
EP2373422A4 (en) 2008-12-04 2017-01-18 Büchi Labortechnik AG Methods and apparatus for moving aliquot samples of fluid
JP2012511725A (en) 2008-12-10 2012-05-24 オールテック・アソシエイツ・インコーポレーテッド Chromatography systems and system components
US8314934B2 (en) * 2009-09-01 2012-11-20 Alltech Associates, Inc. Methods and apparatus for analyzing samples and collecting sample fractions
GB2520111A (en) 2012-02-05 2015-05-13 Biodesy Inc Methods for identifying modulators of Ras using nonlinear techniques
GB2538216A (en) * 2013-06-13 2016-11-16 Biodesy Inc Method of screening candidate biochemical entities targeting a target biochemical entity
EP3237906B8 (en) 2014-12-23 2020-10-28 Bluelight Therapeutics, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
CN108358999A (en) * 2018-01-17 2018-08-03 南京师范大学 A kind of estrogen receptor targeting peptides and its preparation and application
CN111612051B (en) * 2020-04-30 2023-06-20 杭州电子科技大学 Weak supervision target detection method based on graph convolution neural network
EP4066824A1 (en) * 2021-03-31 2022-10-05 Dompe' Farmaceutici S.P.A. Combination therapy for covid-19 vaccination
CN113781542A (en) * 2021-09-23 2021-12-10 Oppo广东移动通信有限公司 Model generation method, depth estimation device and electronic equipment

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1998019162A1 (en) * 1996-10-31 1998-05-07 Novalon Pharmaceutical Corporation Identification of drugs using complementary combinatorial libraries
US5789184A (en) * 1993-03-31 1998-08-04 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
WO1999054728A2 (en) * 1998-04-23 1999-10-28 Karo Bio Usa, Inc. Method of predicting receptor modulating activity

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789184A (en) * 1993-03-31 1998-08-04 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
WO1998019162A1 (en) * 1996-10-31 1998-05-07 Novalon Pharmaceutical Corporation Identification of drugs using complementary combinatorial libraries
WO1999054728A2 (en) * 1998-04-23 1999-10-28 Karo Bio Usa, Inc. Method of predicting receptor modulating activity

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHANG CHING-YI ET AL: "Dissection of the LXXLL nuclear receptor-coactivator interaction motif using combinatorial peptide libraries: Discovery of peptide antagonists of estrogen receptors alpha and beta.", MOLECULAR AND CELLULAR BIOLOGY, vol. 19, no. 12, December 1999 (1999-12-01), pages 8226 - 8239, XP001120691, ISSN: 0270-7306 *
NORRIS ET AL: "Peptide antagonists of the human estrogen receptor", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 285, no. 5428, 1999, pages 744 - 746, XP002113864, ISSN: 0036-8075 *
PAIGE ET AL: "Estrogen receptor (ER) modulators each induce distinct conformational changes in ER.alpha. and ER.beta", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 7, March 1999 (1999-03-01), pages 3999 - 4004, XP002113865, ISSN: 0027-8424 *
WIJAYARATNE ASHINI L ET AL: "Comparative analyses of mechanistic differences among antiestrogens.", ENDOCRINOLOGY, vol. 140, no. 12, December 1999 (1999-12-01), pages 5828 - 5840, XP001120790, ISSN: 0013-7227 *

Also Published As

Publication number Publication date
WO2002004956A9 (en) 2002-10-10
WO2002004956A2 (en) 2002-01-17
US20030224390A1 (en) 2003-12-04
JP2004523726A (en) 2004-08-05
AU2001278901A1 (en) 2002-01-21
EP1360501A2 (en) 2003-11-12
CA2414572A1 (en) 2002-01-17

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