WO2001098510A3 - Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants - Google Patents

Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants Download PDF

Info

Publication number
WO2001098510A3
WO2001098510A3 PCT/US2001/019385 US0119385W WO0198510A3 WO 2001098510 A3 WO2001098510 A3 WO 2001098510A3 US 0119385 W US0119385 W US 0119385W WO 0198510 A3 WO0198510 A3 WO 0198510A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
senescence
dna encoding
transgenic plants
plant lipase
Prior art date
Application number
PCT/US2001/019385
Other languages
French (fr)
Other versions
WO2001098510A2 (en
Inventor
John E Thompson
Tzann-Wei Wang
Katalin Hudak
Yuwen Hong
Original Assignee
Senesco Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/610,104 external-priority patent/US6774284B1/en
Priority to AU2001272950A priority Critical patent/AU2001272950B8/en
Priority to JP2002504658A priority patent/JP2004500886A/en
Priority to IL15347501A priority patent/IL153475A0/en
Priority to CA002413801A priority patent/CA2413801A1/en
Priority to AU7295001A priority patent/AU7295001A/en
Application filed by Senesco Technologies Inc filed Critical Senesco Technologies Inc
Priority to EP01952162A priority patent/EP1294916A2/en
Priority to MXPA02012571A priority patent/MXPA02012571A/en
Priority to BR0111815-3A priority patent/BR0111815A/en
Priority to NZ523280A priority patent/NZ523280A/en
Publication of WO2001098510A2 publication Critical patent/WO2001098510A2/en
Publication of WO2001098510A3 publication Critical patent/WO2001098510A3/en
Priority to IL197268A priority patent/IL197268A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266—Abscission; Dehiscence; Senescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Abstract

Regulation of expression of senescence in plants is achieved by integration of a gene or gene fragment encoding senescence-induced lipase into the plant genome in antisense orientation. The carnation and Arabidopsis genes encoding senescence-induced lipase are identified and the nucleotide sequences are used to modify senescence in transgenic plants.
PCT/US2001/019385 1999-02-16 2001-06-19 Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants WO2001098510A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
NZ523280A NZ523280A (en) 2000-06-19 2001-06-19 DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
JP2002504658A JP2004500886A (en) 2000-06-19 2001-06-19 DNA encoding plant lipase, transgenic plants and methods for controlling senescence in plants
IL15347501A IL153475A0 (en) 2000-06-19 2001-06-19 Dna encoding a plant lipase, transgenic plants and a methods for controlling senescence in plants
CA002413801A CA2413801A1 (en) 2000-06-19 2001-06-19 Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
AU7295001A AU7295001A (en) 2000-06-19 2001-06-19 Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
AU2001272950A AU2001272950B8 (en) 1999-02-16 2001-06-19 DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
EP01952162A EP1294916A2 (en) 2000-06-19 2001-06-19 Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
MXPA02012571A MXPA02012571A (en) 2000-06-19 2001-06-19 Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants.
BR0111815-3A BR0111815A (en) 2000-06-19 2001-06-19 DNA encoding a plant lipase, transgenic plants and method for controlling plant aging
IL197268A IL197268A (en) 2000-06-19 2009-02-26 Isolated dna molecule encoding senescence-induced lipase, a vector comprising it, transgenic plant and a method for inhibiting expression of an endogenous senescence induced lipase gene

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US59777400A 2000-06-19 2000-06-19
US09/597,774 2000-06-19
US09/610,104 US6774284B1 (en) 1998-06-26 2000-07-05 DNA encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
US09/610,104 2000-07-05

Publications (2)

Publication Number Publication Date
WO2001098510A2 WO2001098510A2 (en) 2001-12-27
WO2001098510A3 true WO2001098510A3 (en) 2003-01-09

Family

ID=27082906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/019385 WO2001098510A2 (en) 1999-02-16 2001-06-19 Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants

Country Status (11)

Country Link
EP (1) EP1294916A2 (en)
JP (1) JP2004500886A (en)
CN (1) CN1484705A (en)
AU (1) AU7295001A (en)
BR (1) BR0111815A (en)
CA (1) CA2413801A1 (en)
IL (1) IL153475A0 (en)
MX (1) MXPA02012571A (en)
NZ (1) NZ523280A (en)
RU (1) RU2003101324A (en)
WO (1) WO2001098510A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ994600A0 (en) 2000-09-06 2000-09-28 Agriculture Victoria Services Pty Ltd Manipulation of plant senescene
CN106614586B (en) * 2016-09-30 2019-03-26 中国农业科学院烟草研究所 IDL4 mature polypeptide plant senescence promotor, preparation method and application

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007993A1 (en) * 1993-09-13 1995-03-23 Zeneca Limited Regulation of senescence
WO1996029858A1 (en) * 1995-03-29 1996-10-03 Wisconsin Alumni Research Foundation Transgenic plants with altered senescence characteristics
WO1996035792A1 (en) * 1995-05-09 1996-11-14 Florigene Limited Transgenic carnations exhibit prolonged post-harvest life
WO1997013851A1 (en) * 1995-10-13 1997-04-17 Purdue Research Foundation Improvement of fruit quality by inhibiting production of lipoxygenase in fruits
WO2000009708A1 (en) * 1998-08-17 2000-02-24 Syngenta Limited Dna constructs comprising protease encoding sequences or inhibitors thereof
WO2000049164A1 (en) * 1999-02-16 2000-08-24 Senesco, Inc. Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007993A1 (en) * 1993-09-13 1995-03-23 Zeneca Limited Regulation of senescence
WO1996029858A1 (en) * 1995-03-29 1996-10-03 Wisconsin Alumni Research Foundation Transgenic plants with altered senescence characteristics
WO1996035792A1 (en) * 1995-05-09 1996-11-14 Florigene Limited Transgenic carnations exhibit prolonged post-harvest life
WO1997013851A1 (en) * 1995-10-13 1997-04-17 Purdue Research Foundation Improvement of fruit quality by inhibiting production of lipoxygenase in fruits
WO2000009708A1 (en) * 1998-08-17 2000-02-24 Syngenta Limited Dna constructs comprising protease encoding sequences or inhibitors thereof
WO2000049164A1 (en) * 1999-02-16 2000-08-24 Senesco, Inc. Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 28, no. 6, December 2000 (2000-12-01), pages 775 - 777, ISSN: 0300-5127 *
BROWN J H ET AL: "MOLECULAR SPECIES SPECIFICITY OF PHOSPHOLIPID BREAKDOWN IN MICROSOMAL MEMBRANES OF SENESCING CARNATION FLOWERS", PLANT PHYSIOLOGY, ROCKWILLE, MD, US, vol. 85, no. 3, 1987, pages 679 - 683, XP000925613, ISSN: 0032-0889 *
BURGER L ET AL: "RELATIONSHIP BETWEEN CHANGES IN MEMBRANE PERMEABILITY, RESPIRATION RATE, ACTIVITIES OF LIPASE AND PHOSPHOLIPASE C AND ULTRASTRUCTURE IN SENESCING PETALS OF DIANTHUS CARYOPHYLLUS (CV. WHITE SIM)", SOUTH AFRICAN JOURNAL OF BOTANY - SUID-AFRIKAANS TYDSKRIFT VIR PLANTKUNDE, FOUNDATION FOR EDUCATION, SCIENCE AND TECHNOLOGY, PRETORIA,, SA, vol. 52, no. 3, 1986, pages 195 - 200, XP000925543, ISSN: 0254-6299 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; December 2000 (2000-12-01), THOMPSON J ET AL: "Altered membrane lipase expression delays leaf senescence.", XP002211660, Database accession no. PREV200100186359 *
DATABASE EMBL [online] 14 June 2000 (2000-06-14), "AB06H06 AB Arabidopsis thaliana cDNA 5' similar to lipase, mRNA sequence.", XP002211635, Database accession no. BE038788 *
DATABASE EMBL [online] 3 June 1997 (1997-06-03), "Arabidopsis thaliana chromosome 2 BAC T6B20 genomic sequence, complete", XP002211633, Database accession no. U93215 *
DATABASE EMBL [online] 6 January 1999 (1999-01-06), "Dianthus caryophyllus lipase mRNA, partial cds.", XP002143322, Database accession no. AF026480 *
DATABASE GENESEQ [online] 17 October 2000 (2000-10-17), "Arabidopsis thaliana DNA fragment SEQ ID NO: 25113.", XP002211629, Database accession no. AAC39568 *
DATABASE GENESEQ [online] 17 October 2000 (2000-10-17), "Arabidopsis thaliana protein fragment SEQ ID NO: 25115.", XP002211630, Database accession no. AAG22257 *
DATABASE GENESEQ [online] 18 October 2000 (2000-10-18), "Arabidopsis thaliana DNA fragment SEQ ID NO: 57315.", XP002211631, Database accession no. AAC48392 *
DATABASE GENSEQ [online] 18 October 2000 (2000-10-18), "Arabidopsis thaliana protein fragment SEQ ID NO: 57317.", XP002211632, Database accession no. AAG45635 *
DATABASE SWISSPROT [online] 1 July 1997 (1997-07-01), "Putative lipase", XP002211634, Database accession no. O04340 *
DATABASE SWISSPROT [online] 1 May 1999 (1999-05-01), "Lipase (Fragment).", XP002211628, Database accession no. Q9ZTW1 *
HONG, Y., ET AL.: "An ethylene-induced cDNA encoding a lipase expressed at the onset of senescence", PROCEEDINGS OF THE NATIONAL ACADEMY OD SCIENCES (USA), vol. 97, no. 15, 18 July 2000 (2000-07-18), pages 8717 - 8722, XP002143325 *
HUANG A: "Plant Lipases", PLANT LIPASES, ELSEVIER, AMSTERDAM, NL, 1984, pages 419 - 442, XP002112793 *
THOMPSON JOHN E ET AL: "Lipid metabolism during plant senescence.", PROGRESS IN LIPID RESEARCH, vol. 37, no. 2-3, July 1998 (1998-07-01), pages 119 - 141, XP002211627, ISSN: 0163-7827 *

Also Published As

Publication number Publication date
JP2004500886A (en) 2004-01-15
IL153475A0 (en) 2003-07-06
NZ523280A (en) 2005-02-25
BR0111815A (en) 2004-02-10
AU2001272950B2 (en) 2007-01-11
CA2413801A1 (en) 2001-12-27
AU7295001A (en) 2002-01-02
EP1294916A2 (en) 2003-03-26
WO2001098510A2 (en) 2001-12-27
RU2003101324A (en) 2004-08-20
CN1484705A (en) 2004-03-24
MXPA02012571A (en) 2004-07-30

Similar Documents

Publication Publication Date Title
IL144835A0 (en) Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
WO2001002592A3 (en) Dna encoding a plant deoxyhypusine synthase, a plant eukaryotic initiation factor 5a, transgenic plants and a method for controlling senescence and programmed cell death in plants
WO2001044454A3 (en) Root-specific promoter
EP2336305A3 (en) Cloning of cytochrome P450 genes from Nicotiana
WO2003012052A3 (en) Specific inhibition of gene expression by small double stranded rnas
WO2003004659A3 (en) Recombination systems and a method for removing nucleic acid sequences from the genome of eukaryotic organisms
ATE220722T1 (en) CONTROLLED EXPRESSION OF TRANSGENIC CONSTRUCTS IN PLANT PLASTIDS
WO2002046438A3 (en) Processes and vectors for producing transgenic plants
WO2001081600A3 (en) Transgenic plants
WO2001098510A3 (en) Dna encoding a plant lipase, transgenic plants and a method for controlling senescence in plants
WO2006000319A3 (en) Method for increasing pathogen-resistance in transgenic plants by expression of peroxidase
WO2002044392A3 (en) Dna encoding a plant deoxyhypusine synthase, a plant eukaryotic initiation factor 5a, transgenic plants and a method for controlling senescence programmed and cell death in plants
EP1116794A3 (en) Transgenic plants carrying neoxanthin cleavage enzyme gene
WO2001085963A3 (en) Modification of gene expression in transgenic plants
CA2406820A1 (en) Improvement of flower morphology of plants by targeting mads-box gene
WO2002010362A3 (en) Methods of controlling gene expression and gene silencing
JP2004500886A5 (en)
WO2002027001A3 (en) Plants with masked fertility
WO2001005986A3 (en) Mites-like element and transcriptional activation element
WO2003027257A9 (en) Methods of controlling gene expression and gene silencing
Takano et al. Ectoine synthase gene
CA2178645A1 (en) Controlled expression of transgenic constructs in plant plastids
TH39023B (en) Plant genetic engineering by managing the biosynthesis of lignin.

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: IN/PCT/2002/01241/DE

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 153475

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: PA/a/2002/012571

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 1020027017302

Country of ref document: KR

Ref document number: 2413801

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2002 504658

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 523280

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 2001272950

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001952162

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2003101324

Country of ref document: RU

Kind code of ref document: A

Ref country code: RU

Ref document number: RU A

WWP Wipo information: published in national office

Ref document number: 1020027017302

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 018142885

Country of ref document: CN

WWP Wipo information: published in national office

Ref document number: 2001952162

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 523280

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 523280

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 197268

Country of ref document: IL