WO2001094560A3 - In vitro assembly of sv40 viruses and pseudoviruses - Google Patents

In vitro assembly of sv40 viruses and pseudoviruses Download PDF

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Publication number
WO2001094560A3
WO2001094560A3 PCT/IL2001/000520 IL0100520W WO0194560A3 WO 2001094560 A3 WO2001094560 A3 WO 2001094560A3 IL 0100520 W IL0100520 W IL 0100520W WO 0194560 A3 WO0194560 A3 WO 0194560A3
Authority
WO
WIPO (PCT)
Prior art keywords
pseudoviruses
purified
semi
viruses
pure
Prior art date
Application number
PCT/IL2001/000520
Other languages
French (fr)
Other versions
WO2001094560A2 (en
Inventor
Ariella Oppenheim
Ariela Gordon-Shaag
Original Assignee
Yissum Res Dev Co
Hadasit Med Res Service
Ariella Oppenheim
Ariela Gordon-Shaag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co, Hadasit Med Res Service, Ariella Oppenheim, Ariela Gordon-Shaag filed Critical Yissum Res Dev Co
Priority to AU2001274444A priority Critical patent/AU2001274444A1/en
Publication of WO2001094560A2 publication Critical patent/WO2001094560A2/en
Publication of WO2001094560A3 publication Critical patent/WO2001094560A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/22011—Polyomaviridae, e.g. polyoma, SV40, JC
    • C12N2710/22023—Virus like particles [VLP]

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method for the improved in vitro assembly of pseudoviruses. These viruses or pseudoviruses comprise at least one pure or semi-purified SV40 capsid protein and at least one exogenous constituent selected from nucleic acids, proteins and peptides. In the method of the invention at least one semi-purified or pure SV40 capsid protein is brought into contact with at least one exogenous constituent in the presence of semi-purified or pure PARP [Poly (ADP-ribose) polymerase]. The invention further relates to use of pseudoviruses assembled by the method of the invention, in the preparation of a pharmaceutical composition for the treatment of an individual suffering from pathological disorder.
PCT/IL2001/000520 2000-06-06 2001-06-06 In vitro assembly of sv40 viruses and pseudoviruses WO2001094560A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001274444A AU2001274444A1 (en) 2000-06-06 2001-06-06 In vitro assembly of sv40 viruses and pseudoviruses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL136610 2000-06-06
IL13661000A IL136610A0 (en) 2000-06-06 2000-06-06 In vitro assembly of sv40 viruses and pseudoviruses

Publications (2)

Publication Number Publication Date
WO2001094560A2 WO2001094560A2 (en) 2001-12-13
WO2001094560A3 true WO2001094560A3 (en) 2003-10-23

Family

ID=11074235

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/000520 WO2001094560A2 (en) 2000-06-06 2001-06-06 In vitro assembly of sv40 viruses and pseudoviruses

Country Status (3)

Country Link
AU (1) AU2001274444A1 (en)
IL (1) IL136610A0 (en)
WO (1) WO2001094560A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2572129A1 (en) 2004-07-01 2006-01-12 The Circle For The Promotion Of Science And Engineering Viral particle-like construct and method of forming the same under physiological conditions

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997017456A1 (en) * 1995-11-06 1997-05-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem In vitro construction of sv40 viruses and pseudoviruses

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997017456A1 (en) * 1995-11-06 1997-05-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem In vitro construction of sv40 viruses and pseudoviruses

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GAKEN JOOP A ET AL: "Efficient retroviral infection of mammalian cells is blocked by inhibition of poly(ADP-Ribose) polymerase activity.", JOURNAL OF VIROLOGY, vol. 70, no. 6, 1996, pages 3992 - 4000, XP002183261, ISSN: 0022-538X *

Also Published As

Publication number Publication date
WO2001094560A2 (en) 2001-12-13
AU2001274444A1 (en) 2001-12-17
IL136610A0 (en) 2001-06-14

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