WO2001088547A3 - Standardized oxidized ldl assay - Google Patents
Standardized oxidized ldl assay Download PDFInfo
- Publication number
- WO2001088547A3 WO2001088547A3 PCT/US2001/015214 US0115214W WO0188547A3 WO 2001088547 A3 WO2001088547 A3 WO 2001088547A3 US 0115214 W US0115214 W US 0115214W WO 0188547 A3 WO0188547 A3 WO 0188547A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- assay
- standardized
- oxidized ldl
- linked
- carrier protein
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
Abstract
The oxidation of LDL plays an important role in the development of the pathology of atherosclerosis. The invention is a method to standardize an assay for the presence of OxLDL in patient serum by the use of phosphorocholine (PC), a readily available, highly stable, small molecule standard. PC, alone or linked to a carrier protein, may be used as a competitor or to develop a standard curve in an ELISA assay. Alternatively, PC linked to a carrier protein is bound to a microtiter well and patient sera is assayed for binding to reveal the presence of anti-OxLDL antibodies.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2001259730A AU2001259730A1 (en) | 2000-05-12 | 2001-05-11 | Standardized oxidized ldl assay |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20397800P | 2000-05-12 | 2000-05-12 | |
US60/203,978 | 2000-05-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2001088547A2 WO2001088547A2 (en) | 2001-11-22 |
WO2001088547A3 true WO2001088547A3 (en) | 2003-01-30 |
Family
ID=22756083
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/015214 WO2001088547A2 (en) | 2000-05-12 | 2001-05-11 | Standardized oxidized ldl assay |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2001259730A1 (en) |
WO (1) | WO2001088547A2 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE506615T1 (en) * | 1999-10-26 | 2011-05-15 | Univ California | REAGENTS AND METHODS FOR THE DIAGNOSIS, IMAGING AND TREATMENT OF ATHEROSCLEROTIC DISEASES |
DE602005020474D1 (en) | 2004-04-15 | 2010-05-20 | Athera Biotechnologies Ab | Antibodies to phosphorylcholine conjugates |
AU2012294431B2 (en) | 2011-08-09 | 2017-03-30 | Athera Biotechnologies Ab | Antibodies binding to phosphorylcholine (PC) and/or PC conjugates |
PT2742068T (en) | 2011-08-09 | 2019-07-08 | Dyax Corp | New antibodies against phosphorylcholine |
CN103630695A (en) * | 2013-11-20 | 2014-03-12 | 中国人民解放军南京军区南京总医院 | Chemiluminiscence imaging immunoassay method for simultaneously measuring oxidized lipoprotein (a) and oxidized low-density lipoprotein of human serum |
CN104914255A (en) * | 2015-05-21 | 2015-09-16 | 北京协和洛克生物技术有限责任公司 | Kit for detecting concentration of oxidized low-density lipoprotein in sample and preparation method thereof |
CN105974143A (en) * | 2016-04-08 | 2016-09-28 | 刘庆平 | Enzyme-linked immunosorbent assay kit for detection of human oxidized low-density lipoprotein and preparation method thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998059248A1 (en) * | 1997-06-20 | 1998-12-30 | Leuven Research & Development Vzw | Assays, antibodies, and standards for detection of oxidized and mda-modified low density lipoproteins |
WO1999008109A2 (en) * | 1997-08-07 | 1999-02-18 | The Regents Of The University Of California | Antibodies to oxidation-specific epitopes on lipoprotein and methods for their use in detecting, monitoring and inhibiting the growth of atheroma |
-
2001
- 2001-05-11 WO PCT/US2001/015214 patent/WO2001088547A2/en active Application Filing
- 2001-05-11 AU AU2001259730A patent/AU2001259730A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998059248A1 (en) * | 1997-06-20 | 1998-12-30 | Leuven Research & Development Vzw | Assays, antibodies, and standards for detection of oxidized and mda-modified low density lipoproteins |
WO1999008109A2 (en) * | 1997-08-07 | 1999-02-18 | The Regents Of The University Of California | Antibodies to oxidation-specific epitopes on lipoprotein and methods for their use in detecting, monitoring and inhibiting the growth of atheroma |
Non-Patent Citations (2)
Title |
---|
HORKKO SOHVI ET AL: "Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins.", JOURNAL OF CLINICAL INVESTIGATION, vol. 103, no. 1, January 1999 (1999-01-01), pages 117 - 128, XP002204418, ISSN: 0021-9738 * |
SHAW PETER X ET AL: "Natural antibodies with the T15 idiotype may act in atherosclerosis, apoptotic clearance, and protective immunity.", JOURNAL OF CLINICAL INVESTIGATION, vol. 105, no. 12, June 2000 (2000-06-01), pages 1731 - 1740, XP002204419, ISSN: 0021-9738 * |
Also Published As
Publication number | Publication date |
---|---|
WO2001088547A2 (en) | 2001-11-22 |
AU2001259730A1 (en) | 2001-11-26 |
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