WO2001088547A3 - Standardized oxidized ldl assay - Google Patents

Standardized oxidized ldl assay Download PDF

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Publication number
WO2001088547A3
WO2001088547A3 PCT/US2001/015214 US0115214W WO0188547A3 WO 2001088547 A3 WO2001088547 A3 WO 2001088547A3 US 0115214 W US0115214 W US 0115214W WO 0188547 A3 WO0188547 A3 WO 0188547A3
Authority
WO
WIPO (PCT)
Prior art keywords
assay
standardized
oxidized ldl
linked
carrier protein
Prior art date
Application number
PCT/US2001/015214
Other languages
French (fr)
Other versions
WO2001088547A2 (en
Inventor
Joseph Witztum
Sotirios Tsimikas
Peter X Shaw
Gregg Silverman
Wulf Palinski
Original Assignee
Univ California
Joseph Witztum
Sotirios Tsimikas
Peter X Shaw
Gregg Silverman
Wulf Palinski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Joseph Witztum, Sotirios Tsimikas, Peter X Shaw, Gregg Silverman, Wulf Palinski filed Critical Univ California
Priority to AU2001259730A priority Critical patent/AU2001259730A1/en
Publication of WO2001088547A2 publication Critical patent/WO2001088547A2/en
Publication of WO2001088547A3 publication Critical patent/WO2001088547A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The oxidation of LDL plays an important role in the development of the pathology of atherosclerosis. The invention is a method to standardize an assay for the presence of OxLDL in patient serum by the use of phosphorocholine (PC), a readily available, highly stable, small molecule standard. PC, alone or linked to a carrier protein, may be used as a competitor or to develop a standard curve in an ELISA assay. Alternatively, PC linked to a carrier protein is bound to a microtiter well and patient sera is assayed for binding to reveal the presence of anti-OxLDL antibodies.
PCT/US2001/015214 2000-05-12 2001-05-11 Standardized oxidized ldl assay WO2001088547A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001259730A AU2001259730A1 (en) 2000-05-12 2001-05-11 Standardized oxidized ldl assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20397800P 2000-05-12 2000-05-12
US60/203,978 2000-05-12

Publications (2)

Publication Number Publication Date
WO2001088547A2 WO2001088547A2 (en) 2001-11-22
WO2001088547A3 true WO2001088547A3 (en) 2003-01-30

Family

ID=22756083

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/015214 WO2001088547A2 (en) 2000-05-12 2001-05-11 Standardized oxidized ldl assay

Country Status (2)

Country Link
AU (1) AU2001259730A1 (en)
WO (1) WO2001088547A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA02004244A (en) * 1999-10-26 2002-11-04 Univ California Reagents and methods for diagnosing, imaging and treating atherosclerotic disease.
CA2562550C (en) 2004-04-15 2013-09-24 Athera Biotechnologies Ab Phosphorylcholine conjugates and corresponding antibodies
TWI707873B (en) 2011-08-09 2020-10-21 阿雷拉生物技術Ａｂ公司 New antibodies
AU2012294431B2 (en) 2011-08-09 2017-03-30 Athera Biotechnologies Ab Antibodies binding to phosphorylcholine (PC) and/or PC conjugates
CN103630695A (en) * 2013-11-20 2014-03-12 中国人民解放军南京军区南京总医院 Chemiluminiscence imaging immunoassay method for simultaneously measuring oxidized lipoprotein (a) and oxidized low-density lipoprotein of human serum
CN104914255A (en) * 2015-05-21 2015-09-16 北京协和洛克生物技术有限责任公司 Kit for detecting concentration of oxidized low-density lipoprotein in sample and preparation method thereof
CN105974143A (en) * 2016-04-08 2016-09-28 刘庆平 Enzyme-linked immunosorbent assay kit for detection of human oxidized low-density lipoprotein and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998059248A1 (en) * 1997-06-20 1998-12-30 Leuven Research & Development Vzw Assays, antibodies, and standards for detection of oxidized and mda-modified low density lipoproteins
WO1999008109A2 (en) * 1997-08-07 1999-02-18 The Regents Of The University Of California Antibodies to oxidation-specific epitopes on lipoprotein and methods for their use in detecting, monitoring and inhibiting the growth of atheroma

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998059248A1 (en) * 1997-06-20 1998-12-30 Leuven Research & Development Vzw Assays, antibodies, and standards for detection of oxidized and mda-modified low density lipoproteins
WO1999008109A2 (en) * 1997-08-07 1999-02-18 The Regents Of The University Of California Antibodies to oxidation-specific epitopes on lipoprotein and methods for their use in detecting, monitoring and inhibiting the growth of atheroma

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HORKKO SOHVI ET AL: "Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins.", JOURNAL OF CLINICAL INVESTIGATION, vol. 103, no. 1, January 1999 (1999-01-01), pages 117 - 128, XP002204418, ISSN: 0021-9738 *
SHAW PETER X ET AL: "Natural antibodies with the T15 idiotype may act in atherosclerosis, apoptotic clearance, and protective immunity.", JOURNAL OF CLINICAL INVESTIGATION, vol. 105, no. 12, June 2000 (2000-06-01), pages 1731 - 1740, XP002204419, ISSN: 0021-9738 *

Also Published As

Publication number Publication date
AU2001259730A1 (en) 2001-11-26
WO2001088547A2 (en) 2001-11-22

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