WO2001085780A3 - Nucleic acid binding polypeptides - Google Patents

Nucleic acid binding polypeptides Download PDF

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Publication number
WO2001085780A3
WO2001085780A3 PCT/GB2001/002017 GB0102017W WO0185780A3 WO 2001085780 A3 WO2001085780 A3 WO 2001085780A3 GB 0102017 W GB0102017 W GB 0102017W WO 0185780 A3 WO0185780 A3 WO 0185780A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid binding
binding polypeptides
viral
promoter
Prior art date
Application number
PCT/GB2001/002017
Other languages
French (fr)
Other versions
WO2001085780A2 (en
WO2001085780A8 (en
Inventor
Yen Choo
Christophe Demaison
Michael Moore
Monika Anna Papworth
Lindsey Reynolds
Christopher Graeme Ullman
Mark Isalan
Original Assignee
Gendaq Ltd
Yen Choo
Christophe Demaison
Michael Moore
Monika Anna Papworth
Lindsey Reynolds
Christopher Graeme Ullman
Mark Isalan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0011068A external-priority patent/GB0011068D0/en
Priority claimed from GB0013106A external-priority patent/GB0013106D0/en
Priority claimed from PCT/GB2000/003765 external-priority patent/WO2001025417A2/en
Priority claimed from GB0101446A external-priority patent/GB0101446D0/en
Application filed by Gendaq Ltd, Yen Choo, Christophe Demaison, Michael Moore, Monika Anna Papworth, Lindsey Reynolds, Christopher Graeme Ullman, Mark Isalan filed Critical Gendaq Ltd
Priority to AU2001252428A priority Critical patent/AU2001252428A1/en
Publication of WO2001085780A2 publication Critical patent/WO2001085780A2/en
Publication of WO2001085780A8 publication Critical patent/WO2001085780A8/en
Publication of WO2001085780A3 publication Critical patent/WO2001085780A3/en
Priority to US10/276,608 priority patent/US20040039175A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Plant Pathology (AREA)
  • Communicable Diseases (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

We disclose a polypeptide capable of binding to a nucleic acid comprising a viral nucleotide sequence. Preferably, the viral nucleotide sequence comprises a viral promoter sequence, for example, an HIV promoter or a herpesvirus promoter sequence.
PCT/GB2001/002017 2000-05-08 2001-05-08 Nucleic acid binding polypeptides WO2001085780A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001252428A AU2001252428A1 (en) 2000-05-08 2001-05-08 Nucleic acid binding polypeptides
US10/276,608 US20040039175A1 (en) 2000-05-08 2002-11-07 Modulation of viral gene expression by engineered zinc finger proteins

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GB0011068A GB0011068D0 (en) 2000-05-08 2000-05-08 Molecules
GB0011068.4 2000-05-08
GB0013106A GB0013106D0 (en) 2000-05-30 2000-05-30 Molecules
GB0013106.0 2000-05-30
PCT/GB2000/003765 WO2001025417A2 (en) 1999-10-01 2000-10-02 Dna library and its use in methods of selecting and designing polypeptides
GBPCT/GB00/03765 2000-10-02
GB0101446A GB0101446D0 (en) 2000-05-08 2001-01-19 Molecules
GB0101446.3 2001-01-19

Publications (3)

Publication Number Publication Date
WO2001085780A2 WO2001085780A2 (en) 2001-11-15
WO2001085780A8 WO2001085780A8 (en) 2002-03-21
WO2001085780A3 true WO2001085780A3 (en) 2002-09-06

Family

ID=27447791

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/002017 WO2001085780A2 (en) 2000-05-08 2001-05-08 Nucleic acid binding polypeptides

Country Status (2)

Country Link
AU (1) AU2001252428A1 (en)
WO (1) WO2001085780A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002008286A2 (en) * 2000-07-21 2002-01-31 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
AU2007254251A1 (en) * 2006-05-19 2007-11-29 Sangamo Therapeutics, Inc. Methods and compositions for inactivation of dihydrofolate reductase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995019431A1 (en) * 1994-01-18 1995-07-20 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
WO1998053059A1 (en) * 1997-05-23 1998-11-26 Medical Research Council Nucleic acid binding proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995019431A1 (en) * 1994-01-18 1995-07-20 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
WO1998053059A1 (en) * 1997-05-23 1998-11-26 Medical Research Council Nucleic acid binding proteins

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BEERLI ROGER R ET AL: "Toward controlling gene expression at will: Specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, no. 25, December 1998 (1998-12-01), pages 14628 - 14633, XP002187156, ISSN: 0027-8424 *
GREGER INGO H ET AL: "Transcriptional interference perturbs the binding of Sp1 to the Hiv-1 promoter.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 5, 1 March 1998 (1998-03-01), pages 1294 - 1300, XP002191616, ISSN: 0305-1048 *
IMATAKA H ET AL: "TWO REGULATORY PROTEINS THAT BIND TO THE BASIC TRANSCRIPTION ELEMENT BTE A GC BOX SEQUENCE IN THE PROMOTER REGION OF THE RAT P-4501A1 GENE", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 11, no. 10, 1992, pages 3663 - 3671, XP002194248, ISSN: 0261-4189 *
ISALAN MARK ET AL: "A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter.", NATURE BIOTECHNOLOGY, vol. 19, no. 7, July 2001 (2001-07-01), pages 656 - 660, XP002191617, ISSN: 1087-0156 *
PARROTT C ET AL: "VARIABLE ROLE OF THE LONG TERMINAL REPEAT SP1-BINDING SITES IN HUMAN IMMUNODEFICIENCY VIRUS REPLICATION IN T LYMPHOCYTES", JOURNAL OF VIROLOGY, vol. 65, no. 3, 1991, pages 1414 - 1419, XP001064119, ISSN: 0022-538X *
WU HERREN ET AL: "Building zinc fingers by selection: Toward a therapeutic application.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 92, no. 2, 1995, 1995, pages 344 - 348, XP002191615, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2001085780A2 (en) 2001-11-15
AU2001252428A1 (en) 2001-11-20
WO2001085780A8 (en) 2002-03-21

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