WO2001081616A3 - Method for screening of microorganisms under nutrient limited conditions - Google Patents

Method for screening of microorganisms under nutrient limited conditions Download PDF

Info

Publication number
WO2001081616A3
WO2001081616A3 PCT/US2001/009969 US0109969W WO0181616A3 WO 2001081616 A3 WO2001081616 A3 WO 2001081616A3 US 0109969 W US0109969 W US 0109969W WO 0181616 A3 WO0181616 A3 WO 0181616A3
Authority
WO
WIPO (PCT)
Prior art keywords
screening
limited conditions
microorganisms under
under nutrient
cells
Prior art date
Application number
PCT/US2001/009969
Other languages
French (fr)
Other versions
WO2001081616A2 (en
Inventor
Volker Schellenberger
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24223356&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2001081616(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor Int filed Critical Genencor Int
Priority to EP01924409A priority Critical patent/EP1276894B2/en
Priority to DE60106749T priority patent/DE60106749T3/en
Priority to CA2406465A priority patent/CA2406465C/en
Priority to DK01924409.4T priority patent/DK1276894T4/en
Priority to AT01924409T priority patent/ATE280837T1/en
Priority to AU2001251065A priority patent/AU2001251065A1/en
Publication of WO2001081616A2 publication Critical patent/WO2001081616A2/en
Publication of WO2001081616A3 publication Critical patent/WO2001081616A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Described herein are methods for obtaining from an initial population of cells a plurality of subpopulations of cells having a similar density by growing samples from the initial population in medium having a limiting nutrient. Also provided are methods for using such subpopulation of cells to identify members of an initial population, either spontaneously occurring or induced by mutagenesis, having altered and/or preferred phenotypes.
PCT/US2001/009969 2000-04-21 2001-03-28 Method for screening of microorganisms under nutrient limited conditions WO2001081616A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP01924409A EP1276894B2 (en) 2000-04-21 2001-03-28 Method for screening of microorganisms under nutrient limited conditions
DE60106749T DE60106749T3 (en) 2000-04-21 2001-03-28 METHOD FOR SCREENING MICRO-ORGANISMS UNDER NUTRIENT-LIABLE CONDITIONS
CA2406465A CA2406465C (en) 2000-04-21 2001-03-28 Screening cells for a phenotype using subpopulations having similar cell densities by growing in limiting nutrient medium
DK01924409.4T DK1276894T4 (en) 2000-04-21 2001-03-28 Method for screening microorganisms under nutrient-limited conditions
AT01924409T ATE280837T1 (en) 2000-04-21 2001-03-28 METHOD FOR SCREENING MICROORGANISMS UNDER NUTRIENT-LIMITED CONDITIONS
AU2001251065A AU2001251065A1 (en) 2000-04-21 2001-03-28 Method for screening of microorganisms under nutrient limited conditions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/556,921 US6248541B1 (en) 2000-04-21 2000-04-21 Screening under nutrient limited conditions
US09/556,921 2000-04-21

Publications (2)

Publication Number Publication Date
WO2001081616A2 WO2001081616A2 (en) 2001-11-01
WO2001081616A3 true WO2001081616A3 (en) 2002-07-11

Family

ID=24223356

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009969 WO2001081616A2 (en) 2000-04-21 2001-03-28 Method for screening of microorganisms under nutrient limited conditions

Country Status (7)

Country Link
US (1) US6248541B1 (en)
EP (1) EP1276894B2 (en)
AT (1) ATE280837T1 (en)
AU (1) AU2001251065A1 (en)
CA (1) CA2406465C (en)
DE (1) DE60106749T3 (en)
WO (1) WO2001081616A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070255506A1 (en) * 2006-04-26 2007-11-01 Affymetrix, Inc. System, Method, and Computer Product for Instrument Control and Management of Consumable Resources

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2247012A (en) * 1990-07-26 1992-02-19 Occidental Chem Co Continuous bacterial mutation process
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3688920D1 (en) 1985-07-03 1993-09-30 Genencor Int Hybrid polypeptides and process for their preparation.
ATE165363T1 (en) 1985-08-28 1998-05-15 George Pieczenik METHOD AND MEANS FOR SORTING AND DETERMINING BIOLOGICAL INFORMATION
GB9027836D0 (en) 1990-12-21 1991-02-13 Unilever Plc Enzymes and enzymatic detergent compositions
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5521077A (en) 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
JP4156666B2 (en) 1995-08-11 2008-09-24 ノボザイムス アクティーゼルスカブ Methods for preparing polypeptide variants
GB9521788D0 (en) 1995-10-24 1996-01-03 Univ Leeds DNA repair
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
EP0906420A1 (en) 1996-06-07 1999-04-07 Massachusetts Institute Of Technology Programmed sequential mutagenesis
JPH1066576A (en) 1996-08-07 1998-03-10 Novo Nordisk As Double-stranded dna having protruding terminal and shuffling method using the same
FR2753202A1 (en) 1996-09-06 1998-03-13 Agronomique Inst Nat Rech MULTIRESISTANT BACTERIA, METHODS OF OBTAINING AND USE
AU5195398A (en) 1996-10-25 1998-05-15 Advanced Research And Technology Institute, Inc. Dna sequences encoding fusions of dna repair proteins and uses thereof
CN1208456C (en) 1996-12-20 2005-06-29 诺维信公司 Recombination in vivo
ATE418608T1 (en) 1997-03-18 2009-01-15 Novozymes As METHOD OF CREATING A LIBRARY USING DNA SHUFFLING
BR9808368A (en) 1997-03-18 2000-05-23 Novo Nordisk As Processes for the construction of a library of recombinant homologous polynucleotides, for the identification of a polypeptide of interest, and for the production of a polypeptide of interest.
AU738461B2 (en) 1997-03-18 2001-09-20 Novozymes A/S Shuffling of heterologous DNA sequences
WO1998051802A1 (en) 1997-05-12 1998-11-19 Thermogen, Inc. Method for the stabilization of proteins and the thermostabilized alcohol dehydrogenases produced thereby

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2247012A (en) * 1990-07-26 1992-02-19 Occidental Chem Co Continuous bacterial mutation process
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms

Also Published As

Publication number Publication date
WO2001081616A2 (en) 2001-11-01
EP1276894A2 (en) 2003-01-22
DE60106749T3 (en) 2012-09-20
DE60106749T2 (en) 2006-02-16
ATE280837T1 (en) 2004-11-15
DE60106749D1 (en) 2004-12-02
US6248541B1 (en) 2001-06-19
CA2406465C (en) 2012-02-07
CA2406465A1 (en) 2001-11-01
EP1276894B2 (en) 2011-01-19
AU2001251065A1 (en) 2001-11-07
EP1276894B1 (en) 2004-10-27

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