WO2001081607A3 - Adenovirus vectors with knobless fibers, and their uses - Google Patents

Adenovirus vectors with knobless fibers, and their uses Download PDF

Info

Publication number
WO2001081607A3
WO2001081607A3 PCT/NL2001/000323 NL0100323W WO0181607A3 WO 2001081607 A3 WO2001081607 A3 WO 2001081607A3 NL 0100323 W NL0100323 W NL 0100323W WO 0181607 A3 WO0181607 A3 WO 0181607A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
adenovirus vectors
knobless
binding ligand
provides
Prior art date
Application number
PCT/NL2001/000323
Other languages
French (fr)
Other versions
WO2001081607A2 (en
Inventor
Es Helmuth Hendrikus Gerar Van
Beusechem Victor Willem Van
Original Assignee
Crucell Holland Bv
Vrije Universiteit Medisch Ct
Es Helmuth Hendrikus Gerardus
Beusechem Victor Willem Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holland Bv, Vrije Universiteit Medisch Ct, Es Helmuth Hendrikus Gerardus, Beusechem Victor Willem Van filed Critical Crucell Holland Bv
Priority to AU2001252766A priority Critical patent/AU2001252766A1/en
Priority to EP01926234A priority patent/EP1276888A2/en
Priority to CA002407518A priority patent/CA2407518A1/en
Publication of WO2001081607A2 publication Critical patent/WO2001081607A2/en
Publication of WO2001081607A3 publication Critical patent/WO2001081607A3/en
Priority to US10/282,121 priority patent/US20030082146A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10345—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6045—RNA rev transcr viruses
    • C12N2810/6054—Retroviridae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/80—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/80—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
    • C12N2810/85—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian
    • C12N2810/855—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian from receptors; from cell surface antigens; from cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/80—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
    • C12N2810/85—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian
    • C12N2810/859—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian from immunoglobulins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention provides novel adenoviral vectors, in which the knob of the fiber protein has been removed and replaced with a binding ligand. The invention also provides methods of constructing such vectors and provides uses for such vectors. A modified knobless adenovirus comprising a new binding ligand according to the invention has improved capabilities of entry into specific cell types.
PCT/NL2001/000323 2000-04-26 2001-04-26 Adenovirus vectors with knobless fibers, and their uses WO2001081607A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2001252766A AU2001252766A1 (en) 2000-04-26 2001-04-26 Adenovirus vectors with knobless fibers, and their uses
EP01926234A EP1276888A2 (en) 2000-04-26 2001-04-26 Adenovirus vectors with knobless fibers, and their uses
CA002407518A CA2407518A1 (en) 2000-04-26 2001-04-26 Adenovirus vectors with knobless fibers, and their uses
US10/282,121 US20030082146A1 (en) 2000-04-26 2002-10-28 Adenovirus vectors with knobless fibers, and their uses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20016000P 2000-04-26 2000-04-26
US60/200,160 2000-04-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/282,121 Continuation US20030082146A1 (en) 2000-04-26 2002-10-28 Adenovirus vectors with knobless fibers, and their uses

Publications (2)

Publication Number Publication Date
WO2001081607A2 WO2001081607A2 (en) 2001-11-01
WO2001081607A3 true WO2001081607A3 (en) 2002-06-13

Family

ID=22740577

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2001/000323 WO2001081607A2 (en) 2000-04-26 2001-04-26 Adenovirus vectors with knobless fibers, and their uses

Country Status (4)

Country Link
EP (1) EP1276888A2 (en)
AU (1) AU2001252766A1 (en)
CA (1) CA2407518A1 (en)
WO (1) WO2001081607A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1520303B (en) * 2001-03-27 2013-04-24 纽约大学 Turnor therapy with alphavirus-based and high affinity laminin receptor-targeted vectors
WO2007094653A1 (en) * 2006-02-13 2007-08-23 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patientenzorg Adenovirus particles having a chimeric adenovirus spike protein, use thereof and methods for producing such particles.
EP2248903A1 (en) 2009-04-29 2010-11-10 Universitat Autònoma De Barcelona Methods and reagents for efficient and targeted gene transfer to monocytes and macrophages

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994010323A1 (en) * 1992-11-04 1994-05-11 Imperial Cancer Research Technology Limited Virus with modified binding moiety specific for the target cells
WO1996026281A1 (en) * 1995-02-21 1996-08-29 Genvec, Inc. Chimeric adenoviral fiber protein and methods of using same
WO1997020051A2 (en) * 1995-11-28 1997-06-05 Genvec, Inc. Vectors and methods for gene transfer to cells
WO1998050053A1 (en) * 1997-05-08 1998-11-12 Genetic Therapy, Inc. Gene transfer with adenoviruses having modified fiber proteins
WO1998054346A1 (en) * 1997-05-28 1998-12-03 Genvec, Inc. Alternatively targeted adenovirus
WO1999041359A1 (en) * 1998-02-17 1999-08-19 The Uab Research Foundation Modified adenovirus containing a fiber replacement protein
WO1999060148A2 (en) * 1998-05-20 1999-11-25 Introgene B.V. Display of viral proteins as ligands for cell-surface receptor
WO1999060147A2 (en) * 1998-05-20 1999-11-25 Introgene B.V. Targeted delivery through a cationic amino acid transporter
WO1999064582A2 (en) * 1998-06-12 1999-12-16 Introgene B.V. High-throughput screening of gene function using libraries for functional genomics applications
WO2001002431A1 (en) * 1999-07-06 2001-01-11 Got-A-Gene Ab Recombinant adenovirus

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994010323A1 (en) * 1992-11-04 1994-05-11 Imperial Cancer Research Technology Limited Virus with modified binding moiety specific for the target cells
WO1996026281A1 (en) * 1995-02-21 1996-08-29 Genvec, Inc. Chimeric adenoviral fiber protein and methods of using same
WO1997020051A2 (en) * 1995-11-28 1997-06-05 Genvec, Inc. Vectors and methods for gene transfer to cells
WO1998050053A1 (en) * 1997-05-08 1998-11-12 Genetic Therapy, Inc. Gene transfer with adenoviruses having modified fiber proteins
WO1998054346A1 (en) * 1997-05-28 1998-12-03 Genvec, Inc. Alternatively targeted adenovirus
WO1999041359A1 (en) * 1998-02-17 1999-08-19 The Uab Research Foundation Modified adenovirus containing a fiber replacement protein
WO1999060148A2 (en) * 1998-05-20 1999-11-25 Introgene B.V. Display of viral proteins as ligands for cell-surface receptor
WO1999060147A2 (en) * 1998-05-20 1999-11-25 Introgene B.V. Targeted delivery through a cationic amino acid transporter
WO1999064582A2 (en) * 1998-06-12 1999-12-16 Introgene B.V. High-throughput screening of gene function using libraries for functional genomics applications
WO2001002431A1 (en) * 1999-07-06 2001-01-11 Got-A-Gene Ab Recombinant adenovirus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VAN BEUSECHEM W ET AL: "Recombinant adenovirus vectors with knobless fibers for targeted gene transfer", GENE THER, vol. 7, no. 22, November 2000 (2000-11-01), pages 1940 - 1946, XP001064437 *

Also Published As

Publication number Publication date
CA2407518A1 (en) 2001-11-01
WO2001081607A2 (en) 2001-11-01
EP1276888A2 (en) 2003-01-22
AU2001252766A1 (en) 2001-11-07

Similar Documents

Publication Publication Date Title
MXPA03002704A (en) Fiber cement composite materials using sized cellulose fibers.
MXPA03007956A (en) Fiber reinforced cement composite materials using chemically treated fibers with improved dispersibility.
WO2001075087A8 (en) Subtilisin variants
AU1980600A (en) Agent for dying keratin fibers
NZ336829A (en) Adenoviruses having altered hexon proteins which present epitopes that are not recognised by neutralizing antibodies to the unmodified adenovirus of another serotype
ZA99443B (en) Optical fibre assembly.
AU2001233033A1 (en) Planar waveguides with high refractive index
AU2001294624A1 (en) Recombinant adenovirus vectors that are replication-competent in tert-expressing cells
DE69902010D1 (en) CARDABLE DOUBLE GLASS FIBER MIX
AU2002213079A1 (en) All polymer process compatible optical polymer material
AU6596001A (en) Colorant for fibres containing keratin
WO1998057983A3 (en) Mammalian neuro-growth factor like protein
WO2005027711A3 (en) Capsid-modified adenovirus vectors and methods of using the same
CA2336261A1 (en) Cyclin e2 genes and proteins
WO2002036659A3 (en) Highly-halogenated low optical loss polyester
WO2001049721A3 (en) Bacterial genes and proteins that are essential for cell viability and their uses
WO2001081607A3 (en) Adenovirus vectors with knobless fibers, and their uses
WO2003050238A3 (en) Receptor-targeted adenoviral vectors
MXPA02001100A (en) Impregnated glass fiber strands and products including the same.
IT1303569B1 (en) BACKLIT MONOBLOCK INDEX GROUP.
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
AU1183200A (en) Novel gene encoding brain-specific membrane protein
MXPA02001090A (en) Impregnated glass fiber strands and products including the same.
WO2000031254A3 (en) Apoptosis-inducing factor
WO2002018575A3 (en) Genes expressed in the cell cycle

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2407518

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 10282121

Country of ref document: US

Ref document number: 2001252766

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001926234

Country of ref document: EP

Ref document number: 522319

Country of ref document: NZ

WWP Wipo information: published in national office

Ref document number: 2001926234

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001926234

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP