WO2001079440A3 - Extracellular expression of pectate lyase using bacillus or escherichia coli - Google Patents

Extracellular expression of pectate lyase using bacillus or escherichia coli Download PDF

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Publication number
WO2001079440A3
WO2001079440A3 PCT/DK2001/000235 DK0100235W WO0179440A3 WO 2001079440 A3 WO2001079440 A3 WO 2001079440A3 DK 0100235 W DK0100235 W DK 0100235W WO 0179440 A3 WO0179440 A3 WO 0179440A3
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WO
WIPO (PCT)
Prior art keywords
pectate lyase
bacillus
escherichia coli
extracellular expression
thermotoga maritima
Prior art date
Application number
PCT/DK2001/000235
Other languages
French (fr)
Other versions
WO2001079440A2 (en
Inventor
Mads Eskelund Bjoernvad
Jens Toenne Andersen
Lars Kongsbak
Martin Schuelein
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Priority to AU2001248278A priority Critical patent/AU2001248278A1/en
Priority to EP01921238A priority patent/EP1276854A2/en
Publication of WO2001079440A2 publication Critical patent/WO2001079440A2/en
Publication of WO2001079440A3 publication Critical patent/WO2001079440A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002—Pectate lyase (4.2.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

A transformed bacterial host capable of expressing a pectate lyase enzyme endogenous to a strain of Thermotoga maritima, especially a Bacillus or E.coli host cell, is useful in a method for producing the Thermotoga maritima pectate lyase. The Thermotoga maritima pectate lyase is useful for industrial use, e.g. for treatment of textiles.
PCT/DK2001/000235 2000-04-13 2001-04-06 Extracellular expression of pectate lyase using bacillus or escherichia coli WO2001079440A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001248278A AU2001248278A1 (en) 2000-04-13 2001-04-06 Extracellular expression of pectate lyase using bacillus or escherichia coli
EP01921238A EP1276854A2 (en) 2000-04-13 2001-04-06 Extracellular expression of pectate lyase using bacillus or escherichia coli

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200000621 2000-04-13
DKPA200000621 2000-04-13

Publications (2)

Publication Number Publication Date
WO2001079440A2 WO2001079440A2 (en) 2001-10-25
WO2001079440A3 true WO2001079440A3 (en) 2002-01-24

Family

ID=8159424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2001/000235 WO2001079440A2 (en) 2000-04-13 2001-04-06 Extracellular expression of pectate lyase using bacillus or escherichia coli

Country Status (3)

Country Link
EP (1) EP1276854A2 (en)
AU (1) AU2001248278A1 (en)
WO (1) WO2001079440A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE465951B (en) 1984-10-23 1991-11-25 Perstorp Ab ISOMER OF INOSITOL TRIPHOSPHATE PHARMACEUTICAL STATEMENTS FOR SALT FOR USE AS THERAPEUTIC OR PROPHYLACTIC AGENTS AND COMPOSITIONS THEREOF
CA2521402C (en) 2003-04-04 2015-01-13 Diversa Corporation Pectate lyases, nucleic acids encoding them and methods for making and using them

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0235087A (en) * 1988-07-25 1990-02-05 Central Glass Co Ltd Pectate lyase gene and production of pectate lyase
JPH07163341A (en) * 1993-12-15 1995-06-27 Toyobo Co Ltd Stabilized and modified protein
EP0677584A1 (en) * 1994-04-15 1995-10-18 ENIRICERCHE S.p.A. Stable mutants of D-N-alpha-carbamylase
WO1999027084A1 (en) * 1997-11-24 1999-06-03 Novo Nordisk A/S Novel pectate lyases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0235087A (en) * 1988-07-25 1990-02-05 Central Glass Co Ltd Pectate lyase gene and production of pectate lyase
JPH07163341A (en) * 1993-12-15 1995-06-27 Toyobo Co Ltd Stabilized and modified protein
EP0677584A1 (en) * 1994-04-15 1995-10-18 ENIRICERCHE S.p.A. Stable mutants of D-N-alpha-carbamylase
WO1999027084A1 (en) * 1997-11-24 1999-06-03 Novo Nordisk A/S Novel pectate lyases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE CAPLUS [online] NELSON KAREN E. ET AL.: "Evidence for lateral gene transfer between archaea and bacteria from genome sequence of thermotoga maritima", XP002943792, accession no. STM International Database accession no. 1999:361424 *
DATABASE WPI Derwent World Patents Index; AN 1990-080634, XP002943793, CENTRAL GLASS CO LTD: "Pectic acid lyase gene useful for prodn. of pectic acid lyase - is derived from Erwinia carotovora subsp. carotovora IFPO-14082" *
DATABASE WPI Week 199534, Derwent World Patents Index; AN 1995-260034, XP002943794, TOYOBO KK: "Stabilised denatured protein, esp. sarcosine oxidase - is stabilised by replacing cysteine residues with another amino acid" *

Also Published As

Publication number Publication date
WO2001079440A2 (en) 2001-10-25
AU2001248278A1 (en) 2001-10-30
EP1276854A2 (en) 2003-01-22

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