WO2001079257A3 - Multidrug resistance (mdr) efflux pump polypeptides - Google Patents

Multidrug resistance (mdr) efflux pump polypeptides Download PDF

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Publication number
WO2001079257A3
WO2001079257A3 PCT/US2001/012230 US0112230W WO0179257A3 WO 2001079257 A3 WO2001079257 A3 WO 2001079257A3 US 0112230 W US0112230 W US 0112230W WO 0179257 A3 WO0179257 A3 WO 0179257A3
Authority
WO
WIPO (PCT)
Prior art keywords
mdr
methods
efflux pump
determining whether
multidrug resistance
Prior art date
Application number
PCT/US2001/012230
Other languages
French (fr)
Other versions
WO2001079257A2 (en
Inventor
Deborah Vanriet Davis
Bruce Lee Rogers
Abigail Coffin White
Original Assignee
Phytera Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phytera Inc filed Critical Phytera Inc
Priority to AU2001253508A priority Critical patent/AU2001253508A1/en
Publication of WO2001079257A2 publication Critical patent/WO2001079257A2/en
Publication of WO2001079257A3 publication Critical patent/WO2001079257A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci

Abstract

The invention features methods of determining whether a nucleotide sequence encodes an MDR efflux pump, methods for deleting a desired region of DNA in a bacterial cell, methods for determining whether a test substance inhibits the growth or metabolism of cells of a strain of E. faecalis bacteria having a disruptive mutation in a gene encoding a MDR efflux pump, methods for determining whether a test substance includes a compound that blocks efflux of an antibacterial agent from a cell, and methods for identifying an inhibitor of an MDR pump.
PCT/US2001/012230 2000-04-14 2001-04-12 Multidrug resistance (mdr) efflux pump polypeptides WO2001079257A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001253508A AU2001253508A1 (en) 2000-04-14 2001-04-12 Mdr efflux pumps

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19734900P 2000-04-14 2000-04-14
US60/197,349 2000-04-14

Publications (2)

Publication Number Publication Date
WO2001079257A2 WO2001079257A2 (en) 2001-10-25
WO2001079257A3 true WO2001079257A3 (en) 2002-03-21

Family

ID=22729037

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/012230 WO2001079257A2 (en) 2000-04-14 2001-04-12 Multidrug resistance (mdr) efflux pump polypeptides

Country Status (2)

Country Link
AU (1) AU2001253508A1 (en)
WO (1) WO2001079257A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2253957B1 (en) 2006-03-14 2013-05-15 Oregon Health and Science University Methods for producing an immune response to tuberculosis.
CN105046107B (en) * 2015-08-28 2018-04-20 东北大学 A kind of discovery method of limited die body

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040160A1 (en) * 1996-04-24 1997-10-30 Rijksuniversiteit Te Groningen A prokaryotic protein having functional and structural homology with the human p-glycoprotein encoded by the mdr-1 gene, nucleic acids encoding and cells expressing said protein
WO1998014784A1 (en) * 1996-09-30 1998-04-09 Phytera, Inc. Identification and use of mutant multi-drug resistant cells
WO1998050555A2 (en) * 1997-05-06 1998-11-12 Human Genome Sciences, Inc. Enterococcus faecalis polynucleotides and polypeptides
US5866699A (en) * 1994-07-18 1999-02-02 Hybridon, Inc. Oligonucleotides with anti-MDR-1 gene activity
US5885786A (en) * 1996-04-19 1999-03-23 John Wayne Cancer Institute Methods for screening of substances for inhibition of multidrug resistance
US5989832A (en) * 1995-04-21 1999-11-23 Microcide Pharmaceuticals, Inc. Method for screening for non-tetracycline efflux pump inhibitors
WO2001007034A1 (en) * 1999-07-23 2001-02-01 Colorado State University Research Foundation Multidrug resistance pump inhibitors and uses thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866699A (en) * 1994-07-18 1999-02-02 Hybridon, Inc. Oligonucleotides with anti-MDR-1 gene activity
US5989832A (en) * 1995-04-21 1999-11-23 Microcide Pharmaceuticals, Inc. Method for screening for non-tetracycline efflux pump inhibitors
US5885786A (en) * 1996-04-19 1999-03-23 John Wayne Cancer Institute Methods for screening of substances for inhibition of multidrug resistance
WO1997040160A1 (en) * 1996-04-24 1997-10-30 Rijksuniversiteit Te Groningen A prokaryotic protein having functional and structural homology with the human p-glycoprotein encoded by the mdr-1 gene, nucleic acids encoding and cells expressing said protein
WO1998014784A1 (en) * 1996-09-30 1998-04-09 Phytera, Inc. Identification and use of mutant multi-drug resistant cells
WO1998050555A2 (en) * 1997-05-06 1998-11-12 Human Genome Sciences, Inc. Enterococcus faecalis polynucleotides and polypeptides
WO2001007034A1 (en) * 1999-07-23 2001-02-01 Colorado State University Research Foundation Multidrug resistance pump inhibitors and uses thereof

Also Published As

Publication number Publication date
AU2001253508A1 (en) 2001-10-30
WO2001079257A2 (en) 2001-10-25

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