WO2001075145A3 - Ubiquitin ligase assay - Google Patents

Ubiquitin ligase assay Download PDF

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Publication number
WO2001075145A3
WO2001075145A3 PCT/US2001/010906 US0110906W WO0175145A3 WO 2001075145 A3 WO2001075145 A3 WO 2001075145A3 US 0110906 W US0110906 W US 0110906W WO 0175145 A3 WO0175145 A3 WO 0175145A3
Authority
WO
WIPO (PCT)
Prior art keywords
ubiquitin ligase
ligase assay
assay
assays
relates
Prior art date
Application number
PCT/US2001/010906
Other languages
French (fr)
Other versions
WO2001075145A2 (en
Inventor
Sarkiz D Issakani
Jianing Huang
Julie Sheung
Todd R Pray
Original Assignee
Rigel Pharmaceuticals Inc
Sarkiz D Issakani
Jianing Huang
Julie Sheung
Todd R Pray
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rigel Pharmaceuticals Inc, Sarkiz D Issakani, Jianing Huang, Julie Sheung, Todd R Pray filed Critical Rigel Pharmaceuticals Inc
Priority to EP01924655A priority Critical patent/EP1268847B1/en
Priority to DK01924655T priority patent/DK1268847T3/en
Priority to DE60131262T priority patent/DE60131262T2/en
Priority to JP2001573019A priority patent/JP4095805B2/en
Priority to AU2001251291A priority patent/AU2001251291A1/en
Publication of WO2001075145A2 publication Critical patent/WO2001075145A2/en
Publication of WO2001075145A3 publication Critical patent/WO2001075145A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

Abstract

The invention relates to assays for measuring ubiquitin ligase activity and for identifying modulators of ubiquitin ligase enzymes.
PCT/US2001/010906 2000-04-03 2001-04-03 Ubiquitin ligase assay WO2001075145A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP01924655A EP1268847B1 (en) 2000-04-03 2001-04-03 Ubiquitin ligase assay
DK01924655T DK1268847T3 (en) 2000-04-03 2001-04-03 Ubiquitin ligaseassay
DE60131262T DE60131262T2 (en) 2000-04-03 2001-04-03 Ubiquitin ligase ASSAY
JP2001573019A JP4095805B2 (en) 2000-04-03 2001-04-03 Ubiquitin ligase assay
AU2001251291A AU2001251291A1 (en) 2000-04-03 2001-04-03 Ubiquitin ligase assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/542,497 US6740495B1 (en) 2000-04-03 2000-04-03 Ubiquitin ligase assay
US09/542,497 2000-04-03

Publications (2)

Publication Number Publication Date
WO2001075145A2 WO2001075145A2 (en) 2001-10-11
WO2001075145A3 true WO2001075145A3 (en) 2002-07-25

Family

ID=24164065

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/010906 WO2001075145A2 (en) 2000-04-03 2001-04-03 Ubiquitin ligase assay

Country Status (9)

Country Link
US (5) US6740495B1 (en)
EP (1) EP1268847B1 (en)
JP (1) JP4095805B2 (en)
AT (1) ATE377654T1 (en)
AU (1) AU2001251291A1 (en)
DE (1) DE60131262T2 (en)
DK (1) DK1268847T3 (en)
ES (1) ES2296744T3 (en)
WO (1) WO2001075145A2 (en)

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Also Published As

Publication number Publication date
DE60131262T2 (en) 2008-08-28
US7022493B2 (en) 2006-04-04
DE60131262D1 (en) 2007-12-20
US20050032139A1 (en) 2005-02-10
AU2001251291A1 (en) 2001-10-15
JP4095805B2 (en) 2008-06-04
US6737244B2 (en) 2004-05-18
US7781182B2 (en) 2010-08-24
ATE377654T1 (en) 2007-11-15
EP1268847B1 (en) 2007-11-07
US6740495B1 (en) 2004-05-25
US20060105409A1 (en) 2006-05-18
US20090263831A1 (en) 2009-10-22
JP2003529372A (en) 2003-10-07
ES2296744T3 (en) 2008-05-01
EP1268847A2 (en) 2003-01-02
US20020042083A1 (en) 2002-04-11
WO2001075145A2 (en) 2001-10-11
DK1268847T3 (en) 2008-03-10

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