WO2001073090A3 - Method for modifying lignin composition and increasing in vivo digestibility of forages - Google Patents

Method for modifying lignin composition and increasing in vivo digestibility of forages Download PDF

Info

Publication number
WO2001073090A3
WO2001073090A3 PCT/US2001/009398 US0109398W WO0173090A3 WO 2001073090 A3 WO2001073090 A3 WO 2001073090A3 US 0109398 W US0109398 W US 0109398W WO 0173090 A3 WO0173090 A3 WO 0173090A3
Authority
WO
WIPO (PCT)
Prior art keywords
lignin
methyltransferase
forages
increasing
sense
Prior art date
Application number
PCT/US2001/009398
Other languages
French (fr)
Other versions
WO2001073090A2 (en
Inventor
Richard A Dixon
Dianjing Guo
Original Assignee
Samuel Roberts Noble Found Inc
Richard A Dixon
Dianjing Guo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samuel Roberts Noble Found Inc, Richard A Dixon, Dianjing Guo filed Critical Samuel Roberts Noble Found Inc
Priority to AU4773101A priority Critical patent/AU4773101A/en
Priority to AU2001247731A priority patent/AU2001247731B2/en
Priority to NZ521545A priority patent/NZ521545A/en
Priority to CA2404104A priority patent/CA2404104C/en
Priority to MXPA02010432A priority patent/MXPA02010432A/en
Priority to US10/239,463 priority patent/US20040049802A1/en
Publication of WO2001073090A2 publication Critical patent/WO2001073090A2/en
Publication of WO2001073090A3 publication Critical patent/WO2001073090A3/en
Priority to US12/152,671 priority patent/US7888553B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis

Abstract

Methods for transforming forage legumes or woody plants with a DNA construct comprising at least one open reading frame encoding for a caffeoyl CoA 3-O-methyltransferase enzyme or a Medicago sativa caffeic acid 3-O-methyltransferase enzyme or a fragment thereof in either a sense or antisense orientation under a lignification-associated tissue specific promoter have been found, resulting in the down-regulation of the corresponding homologous gene either through antisense inhibition or sense suppression, as well as reduced lignin content and modified lignin composition in the transgenic plants. The expression of the caffeoyl CoA 3-O-methyltransferase transgene produces an increased syringyl lignin to guaiacyl lignin ratio in the transformed plant, and greatly improved forage digestibility.
PCT/US2001/009398 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages WO2001073090A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU4773101A AU4773101A (en) 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages
AU2001247731A AU2001247731B2 (en) 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages
NZ521545A NZ521545A (en) 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages
CA2404104A CA2404104C (en) 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages
MXPA02010432A MXPA02010432A (en) 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages.
US10/239,463 US20040049802A1 (en) 2001-03-23 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages
US12/152,671 US7888553B2 (en) 2000-03-24 2008-05-14 Method for modifying lignin composition and increasing in vivo digestibility of forages

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19208600P 2000-03-24 2000-03-24
US60/192,086 2000-03-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10239463 A-371-Of-International 2001-03-23
US12/152,671 Division US7888553B2 (en) 2000-03-24 2008-05-14 Method for modifying lignin composition and increasing in vivo digestibility of forages

Publications (2)

Publication Number Publication Date
WO2001073090A2 WO2001073090A2 (en) 2001-10-04
WO2001073090A3 true WO2001073090A3 (en) 2002-05-23

Family

ID=22708181

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009398 WO2001073090A2 (en) 2000-03-24 2001-03-23 Method for modifying lignin composition and increasing in vivo digestibility of forages

Country Status (6)

Country Link
AR (1) AR028289A1 (en)
AU (2) AU4773101A (en)
CA (1) CA2404104C (en)
MX (1) MXPA02010432A (en)
NZ (1) NZ521545A (en)
WO (1) WO2001073090A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007203378B2 (en) 2000-06-14 2011-12-08 Agriculture Victoria Services Pty Ltd Modification of lignin biosynthesis (2)
AUPR041900A0 (en) * 2000-09-29 2000-10-19 Agresearch Limited Manipulation of plant cell walls
US8921653B2 (en) 2001-06-14 2014-12-30 Agriculture Victoria Services Pty Ltd Modification of lignin biosynthesis
FR2833615A1 (en) * 2001-12-14 2003-06-20 Genoplante Valor Evaluating digestibility of fodder plants, useful for strain selection, comprises detecting alleles of the cafeoyl coenzymeA 3-O-methyltransferase gene
AUPS017402A0 (en) 2002-01-25 2002-02-14 International Flower Developments Pty Ltd Genetic sequences and uses therefor
AU2003251522A1 (en) * 2002-06-14 2003-12-31 The Salk Institute For Biological Studies Mutant plant caffeic acid/5-hydroxyferulic acid 3/5-0-methyltransferases (comt), methods for preparation and use thereof
AU2003245772A1 (en) * 2002-06-28 2004-01-19 University Of Guelph Harvest-inducible genes form alfalfa (medicago sativa) and methods of use thereof
US7329798B2 (en) 2002-06-28 2008-02-12 University Of Guelph Harvest-inducible regulatory elements and methods of using same
FR2852326B1 (en) * 2003-03-10 2007-11-30 OBTAINING PLANTS WITH IMPROVED DIGESTIBILITY HAVING INACTIVE PEROXYDASE
EP1512748A1 (en) * 2003-09-03 2005-03-09 Johnson & Johnson Research Pty Limited O-methyltransferases of tetrahydrobenzylisoquinoline alkaloid biosynthesis in papaver somniferum
WO2005102034A2 (en) 2004-04-20 2005-11-03 Syngenta Participations Ag Regulatory sequences for expressing gene products in plant reproductive tissue
US9238818B2 (en) 2004-04-20 2016-01-19 Syngenta Participations Ag Methods and genetic constructs for modification of lignin composition of corn cobs
WO2006012594A2 (en) * 2004-07-24 2006-02-02 The Samuel Roberts Noble Foundation, Inc. Modification of lignin biosynthesis
US8138392B2 (en) 2008-07-14 2012-03-20 The Samuel Roberts Noble Foundation, Inc. Disease resistant plants
US9854778B2 (en) 2011-06-30 2018-01-02 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof
JP6223332B2 (en) * 2011-06-30 2017-11-01 モンサント テクノロジー エルエルシー Alfalfa plant and seed corresponding to transformation event KK179-2, and detection method thereof
CA2877873C (en) * 2012-06-26 2020-11-24 Monsanto Technology Llc Methods and composition for enhanced forage quality
AU2018260469A1 (en) 2017-04-25 2019-11-14 Cellectis Alfalfa with reduced lignin composition
CN111621520A (en) * 2020-06-22 2020-09-04 南京林业大学 Application of masson pine PmCOMT gene

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005160A1 (en) * 1991-09-10 1993-03-18 Zeneca Limited Modification of lignin synthesis in plants
WO1994023044A1 (en) * 1993-04-02 1994-10-13 The Samuel Roberts Noble Foundation, Inc. Method for reducing ligning content in plants
WO1998003535A1 (en) * 1996-07-19 1998-01-29 Purdue Research Foundation Manipulation of lignin composition in plants using a tissue-specific promoter

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005160A1 (en) * 1991-09-10 1993-03-18 Zeneca Limited Modification of lignin synthesis in plants
WO1994023044A1 (en) * 1993-04-02 1994-10-13 The Samuel Roberts Noble Foundation, Inc. Method for reducing ligning content in plants
WO1998003535A1 (en) * 1996-07-19 1998-01-29 Purdue Research Foundation Manipulation of lignin composition in plants using a tissue-specific promoter

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BOUDET A M ET AL: "Lignin Genetic Engineering", MOLECULAR BREEDING, vol. 2, 1996, pages 25 - 39, XP002025844, ISSN: 1380-3743 *
DIANJING G. ET AL.: "Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin", PLANT CELL, vol. 13, January 2001 (2001-01-01), pages 73 - 88, XP002187460 *
INOUE K. ET AL.: "Developmental Expression and Substrate Specificities of Alfalfa Caffeic Acid 3-O-Methyltransferase and Caffeoyl Coenzyme A 3-O-Methyltransferase in Relation to Lignification", PLANT PHYSIOLOGY, vol. 117, 1998, pages 761 - 770, XP002187459 *
ZHONG R. ET AL.: "Dual Methylation Pathway in Lignin Biosynthesis", PLANT CELL, vol. 10, December 1998 (1998-12-01), pages 2033 - 2045, XP002187458 *

Also Published As

Publication number Publication date
CA2404104A1 (en) 2001-10-04
NZ521545A (en) 2004-04-30
AR028289A1 (en) 2003-04-30
MXPA02010432A (en) 2003-04-25
CA2404104C (en) 2012-12-11
WO2001073090A2 (en) 2001-10-04
AU4773101A (en) 2001-10-08
AU2001247731B2 (en) 2006-10-12

Similar Documents

Publication Publication Date Title
WO2001073090A3 (en) Method for modifying lignin composition and increasing in vivo digestibility of forages
Campbell et al. Variation in lignin content and composition (mechanisms of control and implications for the genetic improvement of plants).
Boudet A new view of lignification
Li et al. Improvement of biomass through lignin modification
Baucher et al. Red xylem and higher lignin extractability by down-regulating a cinnamyl alcohol dehydrogenase in poplar
Jackson et al. Improving saccharification efficiency of alfalfa stems through modification of the terminal stages of monolignol biosynthesis
Guo et al. Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferases
US5451514A (en) Modification of lignin synthesis in plants
Boerjan et al. Lignin biosynthesis
Halpin et al. Manipulation of lignin quality by downregulation of cinnamyl alcohol dehydrogenase
Baucher et al. Lignin: genetic engineering and impact on pulping
US7888553B2 (en) Method for modifying lignin composition and increasing in vivo digestibility of forages
Baucher et al. Biosynthesis and genetic engineering of lignin
Chen et al. Multi‐site genetic modulation of monolignol biosynthesis suggests new routes for formation of syringyl lignin and wall‐bound ferulic acid in alfalfa (Medicago sativa L.)
Terashima et al. New preparations of lignin polymer models under conditions that approximate cell wall lignification. II. Structural characterization of the models by thioacidolysis
WO2008005631A2 (en) Genes encoding chavicol/eugenol synthase from the creosote bush larrea tridentata
Ros et al. Structural motifs of syringyl peroxidases predate not only the gymnosperm–angiosperm divergence but also the radiation of tracheophytes
AU2001247731A1 (en) Method for modifying lignin composition and increasing in vivo digestibility of forages
Yan-Ru Recent advances in study of lignin biosynthesis and manipulation
Anterola et al. Multi-site modulation of flux during monolignol formation in loblolly pine (Pinus taeda)
Heath et al. cDNA cloning and differential expression of three caffeic acid O-methyltransferase homologues from perennial ryegrass (Lolium perenne)
Oliveira et al. Feruloyl esterase activity and its role in regulating the feruloylation of maize cell walls
Sederoff et al. Genetic regulation of lignin biosynthesis and the potential modification of wood by genetic engineering in loblolly pine
Chesson Manipulation of fibre degradation: an old theme revisited.
Grand et al. Natural variations and controlled changes in lignification process

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 521545

Country of ref document: NZ

Ref document number: 2404104

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001247731

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: PA/a/2002/010432

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 10239463

Country of ref document: US

122 Ep: pct application non-entry in european phase
WWP Wipo information: published in national office

Ref document number: 521545

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 521545

Country of ref document: NZ

NENP Non-entry into the national phase

Ref country code: JP