WO2001068831A3 - Effective nuclear reprogramming in mammals - Google Patents

Effective nuclear reprogramming in mammals Download PDF

Info

Publication number
WO2001068831A3
WO2001068831A3 PCT/US2001/008371 US0108371W WO0168831A3 WO 2001068831 A3 WO2001068831 A3 WO 2001068831A3 US 0108371 W US0108371 W US 0108371W WO 0168831 A3 WO0168831 A3 WO 0168831A3
Authority
WO
WIPO (PCT)
Prior art keywords
oocyte
genetic material
mammals
introducing
donor genetic
Prior art date
Application number
PCT/US2001/008371
Other languages
French (fr)
Other versions
WO2001068831A2 (en
Inventor
Steven Stice
Original Assignee
Univ Georgia Res Found
Steven Stice
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgia Res Found, Steven Stice filed Critical Univ Georgia Res Found
Priority to CA2403344A priority Critical patent/CA2403344C/en
Priority to EP01918733A priority patent/EP1356031B1/en
Priority to AT01918733T priority patent/ATE517990T1/en
Priority to AU2001245776A priority patent/AU2001245776A1/en
Priority to DK01918733.5T priority patent/DK1356031T3/en
Publication of WO2001068831A2 publication Critical patent/WO2001068831A2/en
Publication of WO2001068831A3 publication Critical patent/WO2001068831A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Casting Devices For Molds (AREA)
  • Molds, Cores, And Manufacturing Methods Thereof (AREA)
  • Stored Programmes (AREA)

Abstract

The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing donor genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is at metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into an oocyte.
PCT/US2001/008371 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals WO2001068831A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2403344A CA2403344C (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals
EP01918733A EP1356031B1 (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals
AT01918733T ATE517990T1 (en) 2000-03-15 2001-03-15 EFFECTIVE NUCLEAR REPROGRAMMING IN MAMMALS
AU2001245776A AU2001245776A1 (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals
DK01918733.5T DK1356031T3 (en) 2000-03-15 2001-03-15 Efficient core reprogramming in mammals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18968600P 2000-03-15 2000-03-15
US60/189,686 2000-03-15

Publications (2)

Publication Number Publication Date
WO2001068831A2 WO2001068831A2 (en) 2001-09-20
WO2001068831A3 true WO2001068831A3 (en) 2003-08-28

Family

ID=22698365

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/008371 WO2001068831A2 (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals

Country Status (7)

Country Link
US (2) US6906238B2 (en)
EP (1) EP1356031B1 (en)
AT (1) ATE517990T1 (en)
AU (1) AU2001245776A1 (en)
CA (1) CA2403344C (en)
DK (1) DK1356031T3 (en)
WO (1) WO2001068831A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9485101A (en) 2000-09-28 2002-04-08 Sangamo Biosciences Inc Nuclear reprogramming using iwsi and related chromatin remodeling atpases
ATE502106T1 (en) * 2000-12-22 2011-04-15 Kyowa Hakko Kirin Co Ltd METHOD FOR CLONING NON-HUMAN MAMMALS USING REPROGRAMMED DONOR CHROMATIN OR DONOR CELLS
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
US7491534B2 (en) 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
CN1379977A (en) * 2001-04-10 2002-11-20 陆长富 Method for constituting clonal embryo of mammal and human clonal embryo constitution
JP3842597B2 (en) * 2001-08-14 2006-11-08 家畜受精卵移植技術研究組合 G1 phase cell collection method and nuclear transfer method using the cell
US20080222745A1 (en) * 2007-03-07 2008-09-11 Utah State University Colcemid-Treatment of Oocytes to enhance Nuclear Transfer Cloning
US20100293626A1 (en) * 2007-11-19 2010-11-18 Seoul National University Industry Foundation Methods for improvement of birth rates in canidae on somatic cell nuclear transfer
JP2011508600A (en) * 2008-01-04 2011-03-17 ソウル ナショナル ユニバーシティ インダストリー ファウンデーション Method for producing transformed replicated dogs

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5213979A (en) 1987-12-30 1993-05-25 W. R. Grace & Co.-Conn. In vitro culture of bovine embryos
IL94184A0 (en) 1989-05-01 1991-01-31 Granada Biosciences Inc In vitro maturation of bovine oocytes
US5160312A (en) 1990-02-09 1992-11-03 W. R. Grace & Co.-Conn. Cryopreservation process for direct transfer of embryos
CA2086330A1 (en) 1990-07-09 1992-01-10 Richard C. Fry Enhanced implantation, development and maintenance of embryos using leukaemia inhibitory factor
US5453366A (en) 1990-07-26 1995-09-26 Sims; Michele M. Method of cloning bovine embryos
US5096822A (en) 1990-07-26 1992-03-17 W. R. Grace & Co.- Conn. Bovine embryo medium
CA2072475A1 (en) 1991-07-01 1993-01-02 Tanja Dominko In vitro maturation of denuded bovine oocytes
US5480772A (en) 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
US5496720A (en) 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1995016770A1 (en) 1993-12-17 1995-06-22 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
GB9417831D0 (en) 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
EP0934403A4 (en) 1996-08-19 2001-03-14 Univ Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
CA2282722A1 (en) 1997-03-06 1998-09-11 Infigen, Inc. Method of cloning animals
GB2340943B (en) 1998-08-28 2000-07-19 Bank Of England Improvements in and relating to sheet material inspection apparatus and methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHASTANT-MAILLARD S. ET AL.: "Clonage et reprogrammation du noyau dans l'embryon préimplantatoire", GYNECOL OBSTET FERTIL., 28 September 2002 (2002-09-28), pages 649 - 658, XP008004095 *
KEEFER C L ET AL: "EFFECT OF FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE DURING BOVINE IN VITRO MATURATION ON DEVELOPMENT FOLLOWING IN VITROFERTILIZATION AND NUCLEAR TRANSFER", MOLECULAR REPRODUCTION AND DEVELOPMENT, LISSS, NEW YORK, NY, US, vol. 36, 1993, pages 469 - 474, XP002937732, ISSN: 1040-452X *
KIKYO NOBUAKI ET AL: "Reprogramming nuclei: Insights from cloning, nuclear transfer and heterokaryons", JOURNAL OF CELL SCIENCE, ESSEX, GB, vol. 113, no. 1, January 2000 (2000-01-01), pages 11 - 20, XP002176693 *
MIYOSHI, K. ET AL.: "Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matures in a protein-free medium", DEVELOPMENTAL BIOLOGY, vol. 1, 11 July 2001 (2001-07-11), pages 12, XP002201739 *
POLEJAEVA I A ET AL: "CLONED PIGS PRODUCED BY NUCLEAR TRANSFER FROM ADULT SOMATIC CELLS", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 407, 7 September 2000 (2000-09-07), pages 86 - 90, XP000986094, ISSN: 0028-0836 *
POLEJAEVA I A ET AL: "NEW ADVANCES IN SOMATIC CELL NUCLEAR TRANSFER: APPLICATION IN TRANSGENESIS", THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 53, no. 1, 1 January 2000 (2000-01-01), pages 117 - 126, XP000940942, ISSN: 0093-691X *
STICE S L ET AL: "CLONING: NEW BREAKTHROUGHS LEADING TO COMMERCIAL OPPORTUNITIES", THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 49, 1998, pages 129 - 138, XP002935721, ISSN: 0093-691X *

Also Published As

Publication number Publication date
CA2403344C (en) 2013-05-21
WO2001068831A2 (en) 2001-09-20
US6906238B2 (en) 2005-06-14
EP1356031A2 (en) 2003-10-29
US20010053550A1 (en) 2001-12-20
US7547818B2 (en) 2009-06-16
AU2001245776A1 (en) 2001-09-24
ATE517990T1 (en) 2011-08-15
EP1356031B1 (en) 2011-07-27
US20040187173A1 (en) 2004-09-23
DK1356031T3 (en) 2011-10-31
CA2403344A1 (en) 2001-09-20

Similar Documents

Publication Publication Date Title
WO2000031237A3 (en) Method of cloning porcine animals
AU6837696A (en) Chimeric animal and method for constructing the same
WO1999005266A3 (en) Trans-species nuclear transfer
WO1998030683A3 (en) Nuclear transfer with differentiated fetal and adult donor cells
EP1017423A4 (en) Cloning pigs using donor nuclei from differentiated cells
BR9506652A (en) Materials and methods for managing hyperacute rejection in xenotransplantation in humans
AU1595195A (en) Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
IL110781A0 (en) Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
WO2001068831A3 (en) Effective nuclear reprogramming in mammals
WO2002010337A3 (en) Method of cloning porcine animals
WO2001038548A3 (en) Vectors capable of immortalizing non-dividing cells and cells immortalized with said vectors
DE69835145D1 (en) Transgenic mice expressing a human antibody
IL141735A (en) Method of culturing embryonic stem cells
WO2002068635A3 (en) Methods of inhibiting expression of a target gene in mammalian cells
WO1998037185A3 (en) Vectors for controlled gene expression
AU1274797A (en) Establishment, maintenance, and transfection of totipotent embryonic stem cells from the embryos of domestic animals
MXPA02006094A (en) A method to produce cloned embryos and adults from cultured cells.
AU1097301A (en) Humanization of non-human, mammalian antibodies
GB2351296A (en) Keratinocyte stem cells
AU6300198A (en) Production of transgenic donor cells for nuclear transfer
WO1994006919A3 (en) Stably-transformed mammalian cells expressing a regulated, inflammatory cyclooxygenase
WO1998032868A3 (en) Dna expression in transfected cells and assays carried out in transfected cells
WO2003040358A1 (en) Somatic cell derived embryonic stem cells and its differentiated cells
WO2000068371A8 (en) Method of cell cultivation
EP0746610A4 (en) Mammalian cloning by embryonic stem cell (esc) nuclear transplantation and esc

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2403344

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001918733

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001918733

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP