WO2001068826A3 - Protoporphyrinogen oxidase ('protox') genes - Google Patents

Protoporphyrinogen oxidase ('protox') genes Download PDF

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Publication number
WO2001068826A3
WO2001068826A3 PCT/EP2001/002753 EP0102753W WO0168826A3 WO 2001068826 A3 WO2001068826 A3 WO 2001068826A3 EP 0102753 W EP0102753 W EP 0102753W WO 0168826 A3 WO0168826 A3 WO 0168826A3
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WO
WIPO (PCT)
Prior art keywords
protox
enzymes
herbicide tolerant
protoporphyrinogen oxidase
present
Prior art date
Application number
PCT/EP2001/002753
Other languages
French (fr)
Other versions
WO2001068826A2 (en
Inventor
Raymonde Fonne-Pfister
Marco Ario De
Sandra Lynn Volrath
Original Assignee
Syngenta Participations Ag
Fonne Pfister Raymonde
Marco Ario De
Sandra Lynn Volrath
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Fonne Pfister Raymonde, Marco Ario De, Sandra Lynn Volrath filed Critical Syngenta Participations Ag
Priority to AU2001260114A priority Critical patent/AU2001260114A1/en
Publication of WO2001068826A2 publication Critical patent/WO2001068826A2/en
Publication of WO2001068826A3 publication Critical patent/WO2001068826A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from maize, soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding a herbicide tolerant form of a plant protox enzyme.
PCT/EP2001/002753 2000-03-14 2001-03-12 Protoporphyrinogen oxidase ('protox') genes WO2001068826A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001260114A AU2001260114A1 (en) 2000-03-14 2001-03-12 Protoporphyrinogen oxidase ("protox") genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52515200A 2000-03-14 2000-03-14
US09/525,152 2000-03-14

Publications (2)

Publication Number Publication Date
WO2001068826A2 WO2001068826A2 (en) 2001-09-20
WO2001068826A3 true WO2001068826A3 (en) 2002-05-23

Family

ID=24092142

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/002753 WO2001068826A2 (en) 2000-03-14 2001-03-12 Protoporphyrinogen oxidase ('protox') genes

Country Status (2)

Country Link
AU (1) AU2001260114A1 (en)
WO (1) WO2001068826A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12123009B2 (en) 2021-11-09 2024-10-22 Monsanto Technology Llc Methods and compositions for PPO herbicide tolerance in plants

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080023768A (en) 2000-03-30 2008-03-14 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Rna sequence-specific mediators of rna interference
WO2002044321A2 (en) 2000-12-01 2002-06-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
ATE542908T1 (en) * 2002-10-15 2012-02-15 Syngenta Participations Ag PLASTIC TRANSFORMATION
DE10333217A1 (en) * 2003-07-22 2005-03-10 Biomay Prod & Handel Polypeptides, encoding nucleic acids from the mold Cladosporium herbarum and their preparation and use in diagnostics and therapy
JP4720223B2 (en) 2004-05-18 2011-07-13 住友化学株式会社 Plants resistant to herbicidal active compounds
UA112969C2 (en) 2010-08-03 2016-11-25 Сібас Юс Ллс PLANT RESISTANT TO ONE OR MORE PPH-INHIBITING HERBICIDES CONTAINING PROTOPORPHYRINOGEN IX OXIDASE (PPX) MUTANT GENE
US20120122223A1 (en) 2010-08-03 2012-05-17 Cibus Us Llc Mutated protoporphyrinogen ix oxidase (ppx) genes
MY169304A (en) 2010-12-16 2019-03-21 Basf Agro Bv Plants having increased tolerance to herbicides
AR091489A1 (en) 2012-06-19 2015-02-11 Basf Se PLANTS THAT HAVE A GREATER TOLERANCE TO HERBICIDES INHIBITORS OF PROTOPORFIRINOGENO OXIDASA (PPO)
US10041087B2 (en) 2012-06-19 2018-08-07 BASF Agro B.V. Plants having increased tolerance to herbicides
CA2920590C (en) 2013-08-12 2023-12-05 BASF Agro B.V. Plants having increased tolerance to herbicides
US10968462B2 (en) 2013-08-12 2021-04-06 BASF Agro B.V. Plants having increased tolerance to herbicides
EP3490366A4 (en) * 2016-07-29 2020-04-22 Monsanto Technology LLC Methods and compositions for gene expression in plants

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO1995034659A1 (en) * 1994-06-16 1995-12-21 Ciba-Geigy Ag Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
WO1997032011A1 (en) * 1996-02-28 1997-09-04 Novartis Ag Dna molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof
WO1998029554A1 (en) * 1996-12-27 1998-07-09 Sumitomo Chemical Co., Ltd. Methods of conferring ppo-inhibiting herbicide resistance to plants by gene manipulation
WO1999046394A1 (en) * 1998-03-11 1999-09-16 Novartis Ag Novel plant plastid promoter sequence

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034659A1 (en) * 1994-06-16 1995-12-21 Ciba-Geigy Ag Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US5767373A (en) * 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
WO1997032011A1 (en) * 1996-02-28 1997-09-04 Novartis Ag Dna molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof
WO1998029554A1 (en) * 1996-12-27 1998-07-09 Sumitomo Chemical Co., Ltd. Methods of conferring ppo-inhibiting herbicide resistance to plants by gene manipulation
WO1999046394A1 (en) * 1998-03-11 1999-09-16 Novartis Ag Novel plant plastid promoter sequence

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL EBI; 19 January 2000 (2000-01-19), VOLRATH SL: "Zea mays protoporphyrinogen IX oxidase mRNA", XP002191472 *
JACOBS N J ET AL: "ASSAY FOR ENZYMATIC PROTO PORPHYRINOGEN OXIDATION A LATE STEP IN HEME SYNTHESIS", ENZYME (BASEL), vol. 28, no. 2-3, 1982, pages 206 - 217, XP001057740, ISSN: 0013-9432 *
MATRINGE M ET AL: "PROTOPORPHYRINOGEN OXIDASE AS A MOLECULAR TARGET FOR DIPHENYL ETHERHERBICIDES", BIOCHEMICAL JOURNAL, PORTLAND PRESS, LONDON, GB, vol. 260, 1989, pages 231 - 235, XP002917381, ISSN: 0264-6021 *
SHERMAN T D ET AL: "PHYSIOLOGICAL BASIS FOR DIFFERENTIAL SENSITIVITIES OF PLANT SPECIES TO PROTOPORPHYRINOGEN OXIDASE-INHIBITING HERBICIDES", PLANT PHYSIOLOGY (BETHESDA), vol. 97, no. 1, 1991, pages 280 - 287, XP001061715, ISSN: 0032-0889 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12123009B2 (en) 2021-11-09 2024-10-22 Monsanto Technology Llc Methods and compositions for PPO herbicide tolerance in plants

Also Published As

Publication number Publication date
AU2001260114A1 (en) 2001-09-24
WO2001068826A2 (en) 2001-09-20

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