WO2001060839A3 - Method for refolding recombinant endostatin - Google Patents

Method for refolding recombinant endostatin Download PDF

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Publication number
WO2001060839A3
WO2001060839A3 PCT/US2001/004547 US0104547W WO0160839A3 WO 2001060839 A3 WO2001060839 A3 WO 2001060839A3 US 0104547 W US0104547 W US 0104547W WO 0160839 A3 WO0160839 A3 WO 0160839A3
Authority
WO
WIPO (PCT)
Prior art keywords
endostatin
refolding
refolded
recombinant endostatin
refolding recombinant
Prior art date
Application number
PCT/US2001/004547
Other languages
French (fr)
Other versions
WO2001060839A2 (en
WO2001060839A9 (en
Inventor
Dong Xie
Paul Grulich
John Erickson
Original Assignee
Us Health
Dong Xie
Paul Grulich
John Erickson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health, Dong Xie, Paul Grulich, John Erickson filed Critical Us Health
Priority to AU2001236951A priority Critical patent/AU2001236951A1/en
Publication of WO2001060839A2 publication Critical patent/WO2001060839A2/en
Publication of WO2001060839A3 publication Critical patent/WO2001060839A3/en
Publication of WO2001060839A9 publication Critical patent/WO2001060839A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A simple and reproducible method for refolding endostatin is provided. Recombinant endostatin isolated from bacterial inclusion bodies of large scale cultures can be refolded using this method. Refolding is accomplished by contacting endostatin with cyclodextrin in an aqueous medium buffered at a neutral or acidic pH (preferably, a pH of about 4 to about 5). The refolded endostatin has similar biological activity, including anti-proliferation activity against stimulated endothelial cells in culture, as native endostatin.
PCT/US2001/004547 2000-02-18 2001-02-12 Method for refolding recombinant endostatin WO2001060839A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001236951A AU2001236951A1 (en) 2000-02-18 2001-02-12 Method for refolding recombinant endostatin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18371200P 2000-02-18 2000-02-18
US60/183,712 2000-02-18

Publications (3)

Publication Number Publication Date
WO2001060839A2 WO2001060839A2 (en) 2001-08-23
WO2001060839A3 true WO2001060839A3 (en) 2002-02-21
WO2001060839A9 WO2001060839A9 (en) 2002-10-17

Family

ID=22674011

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004547 WO2001060839A2 (en) 2000-02-18 2001-02-12 Method for refolding recombinant endostatin

Country Status (2)

Country Link
AU (1) AU2001236951A1 (en)
WO (1) WO2001060839A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105218660B (en) * 2014-07-03 2019-02-12 苏州方舟基因药业有限公司 The novel purification renaturation method and its antitumor application thereof of Recombinant Endostatin
CN111346220B (en) * 2018-12-24 2022-12-09 山东先声生物制药有限公司 Polyethylene glycol modified vascular endothelial inhibin preparation composition

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563057A (en) * 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
WO1999042486A1 (en) * 1998-02-23 1999-08-26 G.D. Searle & Co. Method of producing mouse and human endostatin
WO2000060945A1 (en) * 1999-04-13 2000-10-19 Merck & Co., Inc. SOLUBLE RECOMBINANT ENDOSTATIN AND METHOD OF MAKING SAME FROM $i(STREPTOMYCES SP)

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563057A (en) * 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
WO1999042486A1 (en) * 1998-02-23 1999-08-26 G.D. Searle & Co. Method of producing mouse and human endostatin
WO2000060945A1 (en) * 1999-04-13 2000-10-19 Merck & Co., Inc. SOLUBLE RECOMBINANT ENDOSTATIN AND METHOD OF MAKING SAME FROM $i(STREPTOMYCES SP)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HORSKY J ET AL: "EFFECT OF CYCLODEXTRINS ON AGGREGATION AND REFOLDING OF PROTEINS IN MOLTEN GLOBULE STATE", BIOPHYSICAL JOURNAL, NEW YORK, US, US, 17 February 1996 (1996-02-17), pages A439, XP000574483, ISSN: 0006-3495 *
KARUPPIAH N ET AL: "CYCLODEXTRINS AS PROTEIN FOLDING AIDS", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 211, no. 1, 6 June 1995 (1995-06-06), pages 60 - 66, XP000506286, ISSN: 0006-291X *
ROZEMA D ET AL: "ARTIFICIAL CHAPERONE-ASSITED REFOLDING OF CARBONIC ANHYDRASE BETA", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 271, no. 7, 16 February 1996 (1996-02-16), pages 3478 - 3487, XP000574034, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AU2001236951A1 (en) 2001-08-27
WO2001060839A2 (en) 2001-08-23
WO2001060839A9 (en) 2002-10-17

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