WO2001053532A3 - Barcoded synthetic lethal screening to identify drug targets - Google Patents

Barcoded synthetic lethal screening to identify drug targets Download PDF

Info

Publication number
WO2001053532A3
WO2001053532A3 PCT/US2001/001661 US0101661W WO0153532A3 WO 2001053532 A3 WO2001053532 A3 WO 2001053532A3 US 0101661 W US0101661 W US 0101661W WO 0153532 A3 WO0153532 A3 WO 0153532A3
Authority
WO
WIPO (PCT)
Prior art keywords
synthetic lethal
drug targets
identify drug
clone
barcoded
Prior art date
Application number
PCT/US2001/001661
Other languages
French (fr)
Other versions
WO2001053532A2 (en
Inventor
Charles M Brenner
Daniel D Shoemaker
Original Assignee
Rosetta Inpharmatics Inc
Univ Jefferson
Charles M Brenner
Daniel D Shoemaker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rosetta Inpharmatics Inc, Univ Jefferson, Charles M Brenner, Daniel D Shoemaker filed Critical Rosetta Inpharmatics Inc
Priority to US10/182,209 priority Critical patent/US20040121324A1/en
Priority to EP01942675A priority patent/EP1248860A2/en
Priority to CA002398690A priority patent/CA2398690A1/en
Priority to JP2001553391A priority patent/JP2003535576A/en
Priority to AU2001229589A priority patent/AU2001229589A1/en
Publication of WO2001053532A2 publication Critical patent/WO2001053532A2/en
Publication of WO2001053532A3 publication Critical patent/WO2001053532A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to methods of using synthetic lethal screening techniques to identify drug targets. The methods of the present invention use 'barcoded' libraries of cells, where the library consists of a collection of different mutant clones, each mutant clone bearing a knock-out mutation of a different gene. Each mutant clone has a unique DNA identifier tag, or 'barcode', to allow for quick and convenient identification of the clone and its mutation. The use of such a library allows for rapid, quantitative, sensitive and simple identification of genes which interact with a mutated target gene. So identified genes are promising targets for drug screening.
PCT/US2001/001661 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets WO2001053532A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/182,209 US20040121324A1 (en) 2001-01-18 2001-01-18 Barcoded synthetic lethal screening to identify drug targets
EP01942675A EP1248860A2 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets
CA002398690A CA2398690A1 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets
JP2001553391A JP2003535576A (en) 2000-01-20 2001-01-18 Bar-coded synthetic lethal screening to identify drug targets
AU2001229589A AU2001229589A1 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11746000P 2000-01-20 2000-01-20
US60/117,460 2000-01-20

Publications (2)

Publication Number Publication Date
WO2001053532A2 WO2001053532A2 (en) 2001-07-26
WO2001053532A3 true WO2001053532A3 (en) 2002-02-21

Family

ID=22373061

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/001661 WO2001053532A2 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets

Country Status (5)

Country Link
EP (1) EP1248860A2 (en)
JP (1) JP2003535576A (en)
AU (1) AU2001229589A1 (en)
CA (1) CA2398690A1 (en)
WO (1) WO2001053532A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2159285B1 (en) 2003-01-29 2012-09-26 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP3036033B1 (en) 2013-08-21 2018-06-27 Mycartis N.V. Heterogenous surface functionalization
WO2022133734A1 (en) * 2020-12-22 2022-06-30 Singleron (Nanjing) Biotechnologies, Ltd. Methods and reagents for high-throughput transcriptome sequencing for drug screening
WO2023102610A1 (en) * 2021-12-08 2023-06-15 The University Of Queensland Methods and compositions for multiplexing cell analysis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024603A1 (en) * 1997-11-06 1999-05-20 Fred Hutchinson Cancer Research Center Method for identifying drug targets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024603A1 (en) * 1997-11-06 1999-05-20 Fred Hutchinson Cancer Research Center Method for identifying drug targets

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOISRAME A ET AL: "A mutation in the secretion pathway of the yeast Yarrowia lipolytica that displays synthetic lethality in combination with a mutation affecting the signal recognition particle.", MOLECULAR AND GENERAL GENETICS, vol. 261, no. 4-5, June 1999 (1999-06-01), pages 601 - 609, XP002179867, ISSN: 0026-8925 *
SHOEMAKER D D ET AL: "QUANTITATIVE PHENOTYPIC ANALYSIS OF YEAST DELETION MUTANTS USING A HIGHLY PARALLEL MOLECULAR BAR-CODING STRATEGY", NATURE GENETICS, NEW YORK, NY, US, vol. 14, no. 4, 1 December 1996 (1996-12-01), pages 450 - 456, XP002043431, ISSN: 1061-4036 *
WINZELER ELIZABETH A ET AL: "Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.", SCIENCE (WASHINGTON D C), vol. 285, no. 5429, 6 August 1999 (1999-08-06), pages 901 - 906, XP001025980, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
AU2001229589A1 (en) 2001-07-31
WO2001053532A2 (en) 2001-07-26
JP2003535576A (en) 2003-12-02
EP1248860A2 (en) 2002-10-16
CA2398690A1 (en) 2001-07-26

Similar Documents

Publication Publication Date Title
Michel et al. Functional mapping of yeast genomes by saturated transposition
US20180340939A1 (en) Multiplex analysis of single cell constituents
WO2004060278A3 (en) Methods for rapid identification of pathogens in humans and animals
WO2004052175A3 (en) Methods for rapid identification of pathogens in humans and animals
von Diezmann et al. Let's get physical–mechanisms of crossover interference
PL342114A1 (en) Solid-phase selection of genes undergoind differential expression
WO2001071041A3 (en) Microarray-based analysis of polynucleotide sequence variations
WO2001077392A3 (en) Methods for the survey and genetic analysis of populations
WO1999057311A3 (en) Novel method for the identification of clones conferring a desired biological property from an expression library
WO2007053245A3 (en) Methods and composition to generate unique sequence dna probes, iabeling of dna probes and the use of these probes
AU4965499A (en) Method of using an improved peptide nucleic acid universal library to optimize dna sequence hybridation
WO2002047007A3 (en) Expert system for classification and prediction of genetic diseases
WO2005010494A3 (en) Multiplexed analyte detection
MXPA03007276A (en) Automated assay for identification of individual cells during kinetic assays.
WO2002018635A8 (en) Use of nonviable particles comprising an internal control (ic) nucleic acid
CA2303414A1 (en) Non-competitive co-amplification methods
DE60103428D1 (en) IDENTIFICATION OF CELLS DURING KINETIC TEST SERIES
WO2001053532A3 (en) Barcoded synthetic lethal screening to identify drug targets
WO2001088196A3 (en) Sequence based screening
WO2001009384A3 (en) Serial analysis of genetic alterations
WO2003057913A3 (en) Method for the detection and/or identification of the original animal species in animal matter contained in a sample
MXPA05001947A (en) A genomic approach to identification of novel broad-spectrum antimicrobial peptides from bony fish.
Binan et al. Exploiting molecular barcodes in high-throughput cellular assays
AU2002222939A1 (en) Method of detecting cancer
DE60136746D1 (en) Oligonucleotides for labeling oligonucleotide probes and proteins

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2398690

Country of ref document: CA

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 553391

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2001942675

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001942675

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 10182209

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 2001942675

Country of ref document: EP