WO2001051672A3 - System and method for controlling male fertility - Google Patents

System and method for controlling male fertility Download PDF

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Publication number
WO2001051672A3
WO2001051672A3 PCT/US2001/000573 US0100573W WO0151672A3 WO 2001051672 A3 WO2001051672 A3 WO 2001051672A3 US 0100573 W US0100573 W US 0100573W WO 0151672 A3 WO0151672 A3 WO 0151672A3
Authority
WO
WIPO (PCT)
Prior art keywords
kit
modulate
phosphatidylinositol
kinase
fertility
Prior art date
Application number
PCT/US2001/000573
Other languages
French (fr)
Other versions
WO2001051672A2 (en
Inventor
Peter Blume-Jensen
Tony Hunter
Original Assignee
Salk Inst For Biological Studi
Peter Blume-Jensen
Tony Hunter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Inst For Biological Studi, Peter Blume-Jensen, Tony Hunter filed Critical Salk Inst For Biological Studi
Priority to AU2001229314A priority Critical patent/AU2001229314A1/en
Publication of WO2001051672A2 publication Critical patent/WO2001051672A2/en
Publication of WO2001051672A3 publication Critical patent/WO2001051672A3/en
Priority to US10/192,867 priority patent/US20030084466A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The invention described herein relates to modulation of fertility in male mammals. A genetically altered mouse having a mutation in the Kit/stem cell factor receptor protein is described. In addition, antibodies that specifically bind to a Kit/stem cell factor receptor are described. Methods to diagnose Kit-mediated fertility defects; methods to identify agents that modulate Kit-mediated activation of phosphatidylinositol 3'-kinase and thereby modulate spermatogenesis; and pharmaceuticals that modulate spermatogenesis by effecting Kit-mediated activation of phosphatidylinositol 3'-kinase are also described.
PCT/US2001/000573 2000-01-11 2001-01-08 System and method for controlling male fertility WO2001051672A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001229314A AU2001229314A1 (en) 2000-01-11 2001-01-08 System and method for controlling male fertility
US10/192,867 US20030084466A1 (en) 2000-01-11 2002-07-08 System and method for controlling male fertility

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17562500P 2000-01-11 2000-01-11
US60/175,625 2000-01-11

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/192,867 Continuation US20030084466A1 (en) 2000-01-11 2002-07-08 System and method for controlling male fertility

Publications (2)

Publication Number Publication Date
WO2001051672A2 WO2001051672A2 (en) 2001-07-19
WO2001051672A3 true WO2001051672A3 (en) 2002-06-27

Family

ID=22640983

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/000573 WO2001051672A2 (en) 2000-01-11 2001-01-08 System and method for controlling male fertility

Country Status (2)

Country Link
AU (1) AU2001229314A1 (en)
WO (1) WO2001051672A2 (en)

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BLUME-JENSEN P, JANKNECHT R, HUNTER T.: "The kit receptor promotes cell survival via activation of PI 3-kinase and subsequent Akt-mediated phosphorylation of Bad on Ser136.", CURR BIOL. 1998 JUN 18;8(13):779-82., XP001041061 *
BLUME-JENSEN P, JIANG G, HYMAN R, LEE KF, O'GORMAN S, HUNTER T: "Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3'-kinase is essential for male fertility.", NAT GENET. 2000 FEB;24(2):157-62, XP001040977 *
KISSEL H, TIMOKHINA I, HARDY MP, ROTHSCHILD G, TAJIMA Y, SOARES V, ANGELES M, WHITLOW SR, MANOVA K, BESMER P.: "Point mutation in kit receptor tyrosine kinase reveals essential roles for kit signaling in spermatogenesis and oogenesis without affecting other kit responses", EMBO J. 2000 MAR 15;19(6):1312-26., XP001039906 *
KOZAWA O ET AL: "INVOLVEMENT OF PHOSPHATIDYLINOSITOL 3'-KINASE IN STEM-CELL-FACTOR-INDUCED PHOPHOLIPASE D ACTIVATION AND ARACHIDONIC ACID RELEASE", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 248, no. 1, August 1997 (1997-08-01), pages 149 - 155, XP001040970, ISSN: 0014-2956 *
LEV S, YARDEN Y, GIVOL D.: "Dimerization and activation of the kit receptor by monovalent and bivalent binding of the stem cell factor.", J BIOL CHEM. 1992 AUG 5;267(22):15970-7., XP001041469 *
LOVELAND KL, SCHLATT S.: "Stem cell factor and c-kit in the mammalian testis: lessons originating from Mother Nature's gene knockouts.", J ENDOCRINOL. 1997 JUN;153(3):337-44., XP001040968 *
MAUDUIT C. ET AL.: "Stem cell factor/c-kit system in spermatogenesis.", HUM REPROD UPDATE. 1999 SEP-OCT;5(5):535-45., XP001040998 *
NOLTE RT, ECK MJ, SCHLESSINGER J, SHOELSON SE, HARRISON SC.: "Crystal structure of the PI 3-kinase p85 amino-terminal SH2 domain and its phosphopeptide complexes", NAT STRUCT BIOL. 1996 APR;3(4):364-74., XP001050480 *
SERVE H, HSU YC, BESMER P.: "Tyrosine residue 719 of the c-kit receptor is essential for binding of the P85 subunit of phosphatidylinositol (PI) 3-kinase and for c-kit-associated PI 3-kinase activity in COS-1 cells.", J BIOL CHEM. 1994 FEB 25;269(8):6026-30., XP001041351 *
TAN J C ET AL: "THE DOMINANT W42 SPOTTING PHENOTYPE RESULTS FROM A MISSENSE MUTATION IN THE C-KIT RECEPTOR KINASE", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 247, 12 January 1990 (1990-01-12), pages 209 - 212, XP000608226, ISSN: 0036-8075 *
YEE NS, HSIAU CW, SERVE H, VOSSELLER K, BESMER P.: "Mechanism of down-regulation of c-kit receptor. Roles of receptor tyrosine kinase, phosphatidylinositol 3'-kinase, and protein kinase C", J BIOL CHEM 1994 DEC 16;269(50):31991-8, XP001041381 *
YOSHINAGA K, NISHIKAWA S, OGAWA M, HAYASHI S, KUNISADA T, FUJIMOTO T, NISHIKAWA S.: "Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function.", DEVELOPMENT. 1991 OCT;113(2):689-99., XP001051304 *

Also Published As

Publication number Publication date
WO2001051672A2 (en) 2001-07-19
AU2001229314A1 (en) 2001-07-24

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