WO2001051624A3 - Carboxypeptidase b free of animal products and contaminating enyzme activity - Google Patents

Carboxypeptidase b free of animal products and contaminating enyzme activity Download PDF

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Publication number
WO2001051624A3
WO2001051624A3 PCT/US2001/000008 US0100008W WO0151624A3 WO 2001051624 A3 WO2001051624 A3 WO 2001051624A3 US 0100008 W US0100008 W US 0100008W WO 0151624 A3 WO0151624 A3 WO 0151624A3
Authority
WO
WIPO (PCT)
Prior art keywords
carboxypeptidase
preparations
free
animal products
contaminating
Prior art date
Application number
PCT/US2001/000008
Other languages
French (fr)
Other versions
WO2001051624A2 (en
Inventor
Jose Michael Hanquier
Original Assignee
Lilly Co Eli
Jose Michael Hanquier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli, Jose Michael Hanquier filed Critical Lilly Co Eli
Priority to AU2001229253A priority Critical patent/AU2001229253A1/en
Publication of WO2001051624A2 publication Critical patent/WO2001051624A2/en
Publication of WO2001051624A3 publication Critical patent/WO2001051624A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides highly purified preparations of Carboxypeptidase B. These preparations are generally free of contaminating protease activity, like chymotrypsin or trypsin activity, free of animal products, bacterial cell components and other contaminants that result from conventional purification processes. The highly purified Carboxypeptidase B preparations are particularly suited for the manufacture of recombinant proteins that require delicate proteolytic processes in their manufacture. Compositions containing the present Carboxypeptidase B and such recombinant proteins are also disclosed. Finally, an efficient yeast-based purification system, with high yield downstream processing steps is provided with which the inventive preparations may be made.
PCT/US2001/000008 2000-01-12 2001-01-12 Carboxypeptidase b free of animal products and contaminating enyzme activity WO2001051624A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001229253A AU2001229253A1 (en) 2000-01-12 2001-01-12 Carboxypeptidase b free of animal products and contaminating enyzme activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17578100P 2000-01-12 2000-01-12
US60/175,781 2000-01-12

Publications (2)

Publication Number Publication Date
WO2001051624A2 WO2001051624A2 (en) 2001-07-19
WO2001051624A3 true WO2001051624A3 (en) 2002-03-07

Family

ID=22641594

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/000008 WO2001051624A2 (en) 2000-01-12 2001-01-12 Carboxypeptidase b free of animal products and contaminating enyzme activity

Country Status (2)

Country Link
AU (1) AU2001229253A1 (en)
WO (1) WO2001051624A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1538203B1 (en) * 2003-12-05 2010-01-20 Roche Diagnostics GmbH Recombinantly expressed carboxypeptidase B and purification thereof
JP4411192B2 (en) * 2003-12-05 2010-02-10 エフ．ホフマン−ラ ロシュ アーゲー Recombinantly expressed carboxypeptidase B and purification thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0390327A2 (en) * 1989-02-27 1990-10-03 Eli Lilly And Company improved tissue culture method
WO1996023064A1 (en) * 1995-01-25 1996-08-01 Bio-Technology General Corp. Production of enzymatically active recombinant carboxypeptidase b
US5672496A (en) * 1993-11-16 1997-09-30 Eli Lilly And Company DNA sequences encoding porcine pancreatic carboxypeptidase B

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0390327A2 (en) * 1989-02-27 1990-10-03 Eli Lilly And Company improved tissue culture method
US5672496A (en) * 1993-11-16 1997-09-30 Eli Lilly And Company DNA sequences encoding porcine pancreatic carboxypeptidase B
WO1996023064A1 (en) * 1995-01-25 1996-08-01 Bio-Technology General Corp. Production of enzymatically active recombinant carboxypeptidase b

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AVILES F X ET AL: "SEQUENTIAL HOMOLOGIES BETWEEN PROCARBOXYPEPTIDASE A AND B FROM PORCINE PANCREAS", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,ACADEMIC PRESS, SAN DIEGO, CA,US, vol. 130, no. 1, 16 July 1985 (1985-07-16), pages 97 - 103, XP000940446, ISSN: 0006-291X *
YANG ET AL.: "Separation and characterization of trypsin and carboxypeptidase B-digested products of Met-Lys-human proinsulin", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 76, no. 2, February 1999 (1999-02-01), pages 107 - 114, XP001011512, ISSN: 0273-2289 *

Also Published As

Publication number Publication date
AU2001229253A1 (en) 2001-07-24
WO2001051624A2 (en) 2001-07-19

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