WO2001047638A3 - Integrated microfluidic disc - Google Patents

Integrated microfluidic disc Download PDF

Info

Publication number
WO2001047638A3
WO2001047638A3 PCT/EP2000/013014 EP0013014W WO0147638A3 WO 2001047638 A3 WO2001047638 A3 WO 2001047638A3 EP 0013014 W EP0013014 W EP 0013014W WO 0147638 A3 WO0147638 A3 WO 0147638A3
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic disc
thermocycling reaction
chamber incorporating
integrated microfluidic
nucleic acid
Prior art date
Application number
PCT/EP2000/013014
Other languages
French (fr)
Other versions
WO2001047638A2 (en
Inventor
Nigel Eric Tooke
Per Andersson
Original Assignee
Gyros Ab
Nigel Eric Tooke
Per Andersson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP1999/010347 external-priority patent/WO2000040750A1/en
Priority claimed from GBGB0011425.6A external-priority patent/GB0011425D0/en
Application filed by Gyros Ab, Nigel Eric Tooke, Per Andersson filed Critical Gyros Ab
Priority to US10/168,942 priority Critical patent/US6884395B2/en
Priority to AU35370/01A priority patent/AU3537001A/en
Priority to EP00991793A priority patent/EP1242186B1/en
Priority to JP2001548221A priority patent/JP4791670B2/en
Priority to DE60028819T priority patent/DE60028819T2/en
Publication of WO2001047638A2 publication Critical patent/WO2001047638A2/en
Publication of WO2001047638A3 publication Critical patent/WO2001047638A3/en
Priority to US11/034,539 priority patent/US7332126B2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/088—Passive control of flow resistance by specific surface properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Cereal-Derived Products (AREA)

Abstract

Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
PCT/EP2000/013014 1999-12-23 2000-12-20 Integrated microfluidic disc WO2001047638A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US10/168,942 US6884395B2 (en) 2000-05-12 2000-12-20 Integrated microfluidic disc
AU35370/01A AU3537001A (en) 1999-12-23 2000-12-20 Integrated microfluidic disc
EP00991793A EP1242186B1 (en) 1999-12-23 2000-12-20 Method for processing a nucleic acid template using an integrated microfluidic disc
JP2001548221A JP4791670B2 (en) 1999-12-23 2000-12-20 Integrated microfluidic disk
DE60028819T DE60028819T2 (en) 1999-12-23 2000-12-20 METHOD OF TREATING A MATRIX NUCLEIC ACID USING AN INTEGRATED MICROFLUIDIC PLATE
US11/034,539 US7332126B2 (en) 1999-12-23 2005-01-13 Integrated microfluidic disc

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EPPCT/EP99/10347 1999-12-23
PCT/EP1999/010347 WO2000040750A1 (en) 1998-12-30 1999-12-23 Method for sequencing dna using a microfluidic device
GBGB0011425.6A GB0011425D0 (en) 1999-12-23 2000-05-12 Integrated microfluidic disc
GB0011425.6 2000-05-12

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10168942 A-371-Of-International 2000-12-20
US11/034,539 Continuation US7332126B2 (en) 1999-12-23 2005-01-13 Integrated microfluidic disc

Publications (2)

Publication Number Publication Date
WO2001047638A2 WO2001047638A2 (en) 2001-07-05
WO2001047638A3 true WO2001047638A3 (en) 2002-05-30

Family

ID=26070393

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/013014 WO2001047638A2 (en) 1999-12-23 2000-12-20 Integrated microfluidic disc

Country Status (5)

Country Link
JP (1) JP4791670B2 (en)
AT (1) ATE329691T1 (en)
AU (1) AU3537001A (en)
DE (1) DE60028819T2 (en)
WO (1) WO2001047638A2 (en)

Cited By (1)

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US9530035B2 (en) 2004-03-19 2016-12-27 Applied Biosystems, Llc Methods and systems for using RFID in biological field

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US7014815B1 (en) 1998-10-30 2006-03-21 Burstein Technologies, Inc. Trackable optical discs with concurrently readable nonoperational features
JP2003533681A (en) * 2000-05-15 2003-11-11 テカン・トレーディング・アクチェンゲゼルシャフト Microfluidic device and method for performing cell-based analysis
US7026131B2 (en) 2000-11-17 2006-04-11 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-discs
US7087203B2 (en) 2000-11-17 2006-08-08 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-disc
US7054258B2 (en) 2000-12-08 2006-05-30 Nagaoka & Co., Ltd. Optical disc assemblies for performing assays
US7079468B2 (en) 2000-12-08 2006-07-18 Burstein Technologies, Inc. Optical discs for measuring analytes
US7091034B2 (en) 2000-12-15 2006-08-15 Burstein Technologies, Inc. Detection system for disk-based laboratory and improved optical bio-disc including same
US7759067B2 (en) 2001-03-19 2010-07-20 Gyros Patent Ab Method for determining the amount of an analyte with a disc-shaped microfluidic device
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
US20080288178A1 (en) 2001-08-24 2008-11-20 Applera Corporation Sequencing system with memory
US6726820B1 (en) 2001-09-19 2004-04-27 Applera Corporation Method of separating biomolecule-containing samples with a microdevice with integrated memory
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
WO2003018198A1 (en) 2001-08-28 2003-03-06 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
ATE477054T1 (en) 2001-09-17 2010-08-15 Gyros Patent Ab FUNCTIONAL UNIT ALLOWING CONTROLLED FLOW IN A MICROFLUID DEVICE
US6532997B1 (en) 2001-12-28 2003-03-18 3M Innovative Properties Company Sample processing device with integral electrophoresis channels
JP3952019B2 (en) * 2001-12-28 2007-08-01 株式会社日立ハイテクノロジーズ Extraction apparatus, chemical analysis apparatus, and chemical analysis method
AU2003224586A1 (en) * 2002-04-30 2003-11-17 Gyros Ab Integrated microfluidic device (ea)
US7005265B1 (en) 2002-06-20 2006-02-28 Wenhong Fan Nonenzymatic catalytic signal amplification for nucleic acid hybridization assays
JP2004354364A (en) * 2002-12-02 2004-12-16 Nec Corp Fine particle manipulating unit, chip mounted with the same and detector, and method for separating, capturing and detecting protein
US7390464B2 (en) 2003-06-19 2008-06-24 Burstein Technologies, Inc. Fluidic circuits for sample preparation including bio-discs and methods relating thereto
US7238269B2 (en) * 2003-07-01 2007-07-03 3M Innovative Properties Company Sample processing device with unvented channel
US7776272B2 (en) 2003-10-03 2010-08-17 Gyros Patent Ab Liquid router
US7837947B2 (en) * 2003-12-12 2010-11-23 3M Innovative Properties Company Sample mixing on a microfluidic device
SE0400181D0 (en) 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
TWI295730B (en) * 2004-11-25 2008-04-11 Ind Tech Res Inst Microfluidic chip for sample assay and method thereof
US7947235B2 (en) 2005-04-14 2011-05-24 Gyros Ab Microfluidic device with finger valves
US10816563B2 (en) 2005-05-25 2020-10-27 Boehringer Ingelheim Vetmedica Gmbh System for operating a system for the integrated and automated analysis of DNA or protein
ES2871885T3 (en) 2005-05-25 2021-11-02 Boehringer Ingelheim Vetmedica Gmbh System for the integrated and automated analysis of DNA or proteins and operating procedures of said system
WO2009079051A2 (en) * 2007-09-19 2009-06-25 Nanogen, Inc. Counter-centrifugal force device
WO2012033765A1 (en) 2010-09-07 2012-03-15 The Arizona Board Of Regents On Behalf Of The University Of Arizona Microdroplet-manipulation systems and methods for automated execution of molecular biological protocols
US9993819B2 (en) 2014-12-30 2018-06-12 Stmicroelectronics S.R.L. Apparatus for actuating and reading a centrifugal microfluidic disk for biological and biochemical analyses, and use of the apparatus
CA3035141A1 (en) 2016-10-07 2018-04-12 Boehringer Ingelheim Vetmedica Gmbh Method and analysis system for testing a sample
DK3523035T3 (en) 2016-10-07 2020-10-12 Boehringer Ingelheim Vetmedica Gmbh ANALYSIS DEVICE AND METHOD OF TESTING A SAMPLE
JP7317026B2 (en) 2018-01-30 2023-07-28 ライフ テクノロジーズ コーポレーション Instruments, devices and consumables for use in smart molecular analysis system workflows

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US5863708A (en) * 1994-11-10 1999-01-26 Sarnoff Corporation Partitioned microelectronic device array
WO2000040750A1 (en) * 1998-12-30 2000-07-13 Gyros Ab Method for sequencing dna using a microfluidic device
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Patent Citations (4)

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US5863708A (en) * 1994-11-10 1999-01-26 Sarnoff Corporation Partitioned microelectronic device array
WO1997021090A1 (en) * 1995-12-05 1997-06-12 Gamera Bioscience Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
US6117630A (en) * 1997-10-30 2000-09-12 Motorola, Inc. Molecular detection apparatus and method
WO2000040750A1 (en) * 1998-12-30 2000-07-13 Gyros Ab Method for sequencing dna using a microfluidic device

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Publication number Priority date Publication date Assignee Title
US9530035B2 (en) 2004-03-19 2016-12-27 Applied Biosystems, Llc Methods and systems for using RFID in biological field

Also Published As

Publication number Publication date
JP2003520582A (en) 2003-07-08
DE60028819T2 (en) 2007-01-11
DE60028819D1 (en) 2006-07-27
WO2001047638A2 (en) 2001-07-05
JP4791670B2 (en) 2011-10-12
ATE329691T1 (en) 2006-07-15
AU3537001A (en) 2001-07-09

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