WO2001044852A3 - Microscopy method and device - Google Patents

Microscopy method and device Download PDF

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Publication number
WO2001044852A3
WO2001044852A3 PCT/EP2000/012120 EP0012120W WO0144852A3 WO 2001044852 A3 WO2001044852 A3 WO 2001044852A3 EP 0012120 W EP0012120 W EP 0012120W WO 0144852 A3 WO0144852 A3 WO 0144852A3
Authority
WO
WIPO (PCT)
Prior art keywords
image
sample
dimensions
recorded
focal plane
Prior art date
Application number
PCT/EP2000/012120
Other languages
German (de)
French (fr)
Other versions
WO2001044852A2 (en
Inventor
Achim Kirsch
Juergen Mueller
Original Assignee
Evotec Biosystems Ag
Achim Kirsch
Juergen Mueller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Biosystems Ag, Achim Kirsch, Juergen Mueller filed Critical Evotec Biosystems Ag
Priority to DE20023019U priority Critical patent/DE20023019U1/en
Publication of WO2001044852A2 publication Critical patent/WO2001044852A2/en
Publication of WO2001044852A3 publication Critical patent/WO2001044852A3/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G02B21/20—Binocular arrangements
    • G02B21/22—Stereoscopic arrangements

Abstract

The invention provides that in order to optically image a sample (140) in three-dimensions, the entire sample (140) is uniformly illuminated, a conventional first partial image is recorded, and the sample (140) is subjected to a laterally structured illumination in a focal plane. The illumination of the individual points of the focal plane is varied according to predetermined temporal sequences. A second partial image is recorded by detecting the light emitted by the object points that are not illuminated in the confocal plane. An image of the probe (140) that is spatially resolved in three-dimensions is established from the first and second partial images. The invention also relates to optical imaging systems used for carrying out this imaging method.
PCT/EP2000/012120 1999-12-15 2000-12-01 Microscopy method and device WO2001044852A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE20023019U DE20023019U1 (en) 1999-12-15 2000-12-01 Microscopy device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19960583.1 1999-12-15
DE1999160583 DE19960583A1 (en) 1999-12-15 1999-12-15 Method and device for microscopy

Publications (2)

Publication Number Publication Date
WO2001044852A2 WO2001044852A2 (en) 2001-06-21
WO2001044852A3 true WO2001044852A3 (en) 2001-12-06

Family

ID=7932808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012120 WO2001044852A2 (en) 1999-12-15 2000-12-01 Microscopy method and device

Country Status (2)

Country Link
DE (2) DE19960583A1 (en)
WO (1) WO2001044852A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4334801B2 (en) 2000-03-06 2009-09-30 オリンパス株式会社 Pattern forming member applied to sectioning image observation apparatus and sectioning image observation apparatus using the same
US6778724B2 (en) 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
WO2005022147A1 (en) 2003-08-28 2005-03-10 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
DE102005040471B4 (en) * 2005-08-26 2007-06-21 Leica Microsystems (Schweiz) Ag microscope
US7593156B2 (en) 2005-08-26 2009-09-22 Leica Microsystems (Schweiz) Ag Microscope with micro-mirrors for optional deflection and/or beam splitting
DE102006022590C5 (en) * 2006-05-15 2010-05-12 Leica Microsystems (Schweiz) Ag Lighting unit for a microscope
DE102006022592B4 (en) * 2006-05-15 2008-02-07 Leica Microsystems (Schweiz) Ag Microscope with lighting unit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0911667A1 (en) * 1997-10-22 1999-04-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Programmable spatially light modulated microscope and microscopy method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0485803B1 (en) * 1990-11-10 1996-05-01 Grosskopf, Rudolf, Dr.-Ing. Optical scanning device with confocal beam path using a light source array and a detector array
US5587832A (en) * 1993-10-20 1996-12-24 Biophysica Technologies, Inc. Spatially light modulated confocal microscope and method
DE9406545U1 (en) * 1994-04-20 1994-11-03 Max Planck Gesellschaft Contrast device for microscopy
GB9603788D0 (en) * 1996-02-22 1996-04-24 Isis Innovation Confocal microscope
DE19824460A1 (en) * 1998-05-30 1999-12-02 Zeiss Carl Jena Gmbh Arrangement and method for the microscopic generation of object images

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0911667A1 (en) * 1997-10-22 1999-04-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Programmable spatially light modulated microscope and microscopy method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JUSKAITIS R ET AL: "EFFICIENT REAL-TIME CONFOCAL MICROSCOPY WITH WHITE LIGHT SOURCES", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 383, 31 October 1996 (1996-10-31), pages 804 - 806, XP000877459, ISSN: 0028-0836 *
NEIL M A A ET AL: "Real time 3D fluorescence microscopy by two beam interference illumination", OPTICS COMMUNICATIONS,NL,NORTH-HOLLAND PUBLISHING CO. AMSTERDAM, vol. 153, no. 1-3, 15 July 1998 (1998-07-15), pages 1 - 4, XP004146317, ISSN: 0030-4018 *

Also Published As

Publication number Publication date
WO2001044852A2 (en) 2001-06-21
DE20023019U1 (en) 2002-11-07
DE19960583A1 (en) 2001-07-05

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