WO2001044499B1 - Modified labeled complement components for immunoassays - Google Patents

Modified labeled complement components for immunoassays

Info

Publication number
WO2001044499B1
WO2001044499B1 PCT/US2000/041081 US0041081W WO0144499B1 WO 2001044499 B1 WO2001044499 B1 WO 2001044499B1 US 0041081 W US0041081 W US 0041081W WO 0144499 B1 WO0144499 B1 WO 0144499B1
Authority
WO
WIPO (PCT)
Prior art keywords
poly
reagent
polypeptide linkers
group
complement
Prior art date
Application number
PCT/US2000/041081
Other languages
French (fr)
Other versions
WO2001044499A9 (en
WO2001044499A1 (en
Inventor
Stanley Paul Racis
Steve Sinka
Original Assignee
Diagen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagen Corp filed Critical Diagen Corp
Priority to AU43010/01A priority Critical patent/AU4301001A/en
Publication of WO2001044499A1 publication Critical patent/WO2001044499A1/en
Publication of WO2001044499B1 publication Critical patent/WO2001044499B1/en
Publication of WO2001044499A9 publication Critical patent/WO2001044499A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Rehabilitation Therapy (AREA)
  • Rheumatology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention is in the field of immunologic serological in vitro diagnostics. One aspect of the invention is the reagent itself. The reagent is a polypeptide modified complement componentthat is conjugated to one or more signal emitting molecules, such that it facilitates reactions in assays targeting the presence of analytes in samples. A second aspect of the invention is a method for making the reagent. A third aspect of the invention involves the use of the reagent to eliminate materials, time and costs in many types of immunoserological assays. The invention further includes a kit for detecting the presence of analytes in samples.

Claims

AMENDED CLAIMS[received by the international Bureau on 12 July 2001 (12.07.01); original claims 3 and 5 amended; remaining claims unchanged (1 page)]
1. A reagent for use in immunoassays comprising: a complement component; one or more polypeptide linkers bound to said component to form a reaction product; and one or more signal-emitting molecule bound to said reaction product to form a reagent.
2. The reagent of claim 1 wherein said complement component is selected from the group consisting of Clq and C3b.
3. The reagent of claim 1 wherein said complement component is selected from the group consisting of human complement and synthetic complement.
4. The reagent of claim 1 wherein said one or more polypeptide linkers is selected from the group consisting of poly-1-lysine, poly-1-alanine, poly-1-arginine, poly-1-cysteine, poly-1- glutamate, poly-1-glycine, poly-1-asparagine, poly-1-serine, poly-1-glutamine, poly-1-tyrosine, poly-1-histidine, poly-1-leucine, poly-1-proline, poly-1-threonine, poly-1-tryptophan, poly-1- phenylalanine, poly-1-ornithine, poly-1-hydroxyproline, poly-1-glutamic acid and poly-1- isoleucine.
5. The reagent of claim 1 wherein said one or more polypeptide linkers is selected from the group consisting of human polypeptide linkers, synthetic polypeptide linkers, and commercially available polypeptide linkers.
6. The reagent of claim 1 wherein said one or more signal-emitting molecules is selected from the group consisting of fluorochromes, enzymes, radioisotopes, chemiluminescent compounds and magnetic compounds.
7. The reagent of claim 1 wherein said one or more signal-emitting molecules is either synthetically made or commercially available.
8. A method for making a reagent comprising: binding one or more first signal emitting molecules to one or more polypeptide linkers to form one or more labeled polypeptide linkers; and conjugating said one or more labeled polypeptide linkers to a complement component to form a reaction product.
PCT/US2000/041081 1999-12-16 2000-10-05 Modified labeled complement components for immunoassays WO2001044499A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU43010/01A AU4301001A (en) 1999-12-16 2000-10-05 Modified labeled complement components for immunoassays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17116199P 1999-12-16 1999-12-16
US60/171,161 1999-12-16

Publications (3)

Publication Number Publication Date
WO2001044499A1 WO2001044499A1 (en) 2001-06-21
WO2001044499B1 true WO2001044499B1 (en) 2002-05-10
WO2001044499A9 WO2001044499A9 (en) 2002-06-06

Family

ID=22622777

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/041081 WO2001044499A1 (en) 1999-12-16 2000-10-05 Modified labeled complement components for immunoassays

Country Status (2)

Country Link
AU (1) AU4301001A (en)
WO (1) WO2001044499A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007530913A (en) * 2003-07-10 2007-11-01 ブラインド・ピッグ・プロテオミクス・インコーポレーテッド Universal detection of binding
CN105301235A (en) * 2015-11-16 2016-02-03 北京中航赛维生物科技有限公司 Kit used for quantitative determination anti-nucleosome antibody Ig G via magnetic micro particle chemiluminiscence, and preparation method and detection method thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035995A (en) * 1984-10-02 1991-07-30 Calpis Food Industry Co., Ltd. Test method involving substance-conjugated complement component C1q

Also Published As

Publication number Publication date
AU4301001A (en) 2001-06-25
WO2001044499A9 (en) 2002-06-06
WO2001044499A1 (en) 2001-06-21

Similar Documents

Publication Publication Date Title
ATE93628T1 (en) PERIDININ CHLOROPHYLL COMPLEX AS A FLUORESCENT IDENTIFIER.
US5332679A (en) Method for specific binding assays using a releasable ligand
US4150033A (en) Reagent suitable for enzyme immuno assay
AU1706892A (en) Assay methods utilizing induced luminescence
ZA941084B (en) Immobilization of specific binding assay reagents
ATE213837T1 (en) POLYPEPTIDE-DENDRIMER COMPLEXES
CA2000545A1 (en) Process and reagent for the determination of a specifically bindable substance
AU582293B2 (en) Heterogeneous specific binding assay employing an aggregatable binding reagent
EP0214847A3 (en) Squarate dye conjugate, process for making it and its use in assay methods and kits
ATE22730T1 (en) CHROMATOGRAPHIC ENZYME IMMUNO METHOD.
AU8814191A (en) Cell-free receptor binding assays, the production and use thereof
GB8422452D0 (en) Assay
ES8503133A1 (en) Tricyclic antidepressant drug immunogens, antibodies, labeled conjugates, and related derivatives.
JPH05504481A (en) Improved antibody-enzyme direct conjugate and method for producing the same
US5952185A (en) Photo-activatable biotin derivatives and their use to reduce interference in immunoassays
IL140413A0 (en) A complex of an enzyme, a protein and a carrier
AU4380789A (en) Method and reagents for detecting amphetamine and/or d-methamphetamine in biological samples
AU3084495A (en) Glycated proteins assay
WO2002029409A3 (en) Method of assaying pyrrole-containing biological compounds
DE69727009D1 (en) Reagents for a Lysergic Acid Diethylamide Immunoassay
WO2001044499B1 (en) Modified labeled complement components for immunoassays
AU5141798A (en) Methods and reagents for determining protein s
CA2167321A1 (en) Reagents and methods for the detection and quantification of testosterone in fluid samples
GB2200448B (en) Targetted liposomes and their use in immunoassay
AU5752186A (en) Method for measuring free ligands in biological fluids, and assay for measuring same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGE 19, DESCRIPTION, REPLACED BY A NEW PAGE 19; PAGES 25 AND 28, CLAIMS, REPLACED BY NEW PAGES 25 AND 28; PAGES 1/3-3/3, DRAWINGS, REPLACED BY NEW PAGES 1/3-3/3; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP