WO2001044456A3 - Modification de micro-organismes - Google Patents

Modification de micro-organismes Download PDF

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Publication number
WO2001044456A3
WO2001044456A3 PCT/GB2000/004795 GB0004795W WO0144456A3 WO 2001044456 A3 WO2001044456 A3 WO 2001044456A3 GB 0004795 W GB0004795 W GB 0004795W WO 0144456 A3 WO0144456 A3 WO 0144456A3
Authority
WO
WIPO (PCT)
Prior art keywords
micro
organisms
gene
gene function
organism
Prior art date
Application number
PCT/GB2000/004795
Other languages
English (en)
Other versions
WO2001044456A2 (fr
Inventor
Catherine Elizabeth Dunn Rees
Philip John Hill
Christine Elizabeth Ruth Dodd
Laurence Stephen Tiley
Original Assignee
Univ Nottingham
Univ Cambridge Tech
Catherine Elizabeth Dunn Rees
Philip John Hill
Christine Elizabeth Ruth Dodd
Laurence Stephen Tiley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nottingham, Univ Cambridge Tech, Catherine Elizabeth Dunn Rees, Philip John Hill, Christine Elizabeth Ruth Dodd, Laurence Stephen Tiley filed Critical Univ Nottingham
Priority to AU21966/01A priority Critical patent/AU2196601A/en
Publication of WO2001044456A2 publication Critical patent/WO2001044456A2/fr
Publication of WO2001044456A3 publication Critical patent/WO2001044456A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/126—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes involving RNAse P

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés de modification de micro-organismes, permettant particulièrement de contrôler les micro-organismes. Ces procédés consistent à introduire dans un micro-organisme cible un vecteur comprenant une forme antisens de la partie entière d'un ou plusieurs gènes cible présents normalement dans ce micro-organisme. Après infection du micro-organisme par le vecteur et l'expression de la forme antisens de ce gène ou d'une partie de ce gène dans l'hôte, on évite ou inhibe la traduction du gène cible et les propriétés des micro-organismes modifiés ce qui garantit le contrôle des micro-organismes.
PCT/GB2000/004795 1999-12-17 2000-12-15 Modification de micro-organismes WO2001044456A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU21966/01A AU2196601A (en) 1999-12-17 2000-12-15 Modifying micro-organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9929744.2 1999-12-17
GBGB9929744.2A GB9929744D0 (en) 1999-12-17 1999-12-17 Modifying micro-organisms

Publications (2)

Publication Number Publication Date
WO2001044456A2 WO2001044456A2 (fr) 2001-06-21
WO2001044456A3 true WO2001044456A3 (fr) 2001-12-27

Family

ID=10866437

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004795 WO2001044456A2 (fr) 1999-12-17 2000-12-15 Modification de micro-organismes

Country Status (3)

Country Link
AU (1) AU2196601A (fr)
GB (1) GB9929744D0 (fr)
WO (1) WO2001044456A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007003148A1 (de) * 2007-01-15 2008-07-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Gentechnisch modifizierte Bakteriophagen, insbesondere zur Bekämpfung von pathogenen Prokaryonten bzw. ihrer pathologischen Wirkung, sowie deren Verwendung und Herstellung

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2078716A1 (fr) * 1992-09-21 1994-03-22 Joyce De Azavedo Proteine de e. coli enterohemorragique de fixation et de destruction
US5552144A (en) * 1992-01-22 1996-09-03 Microcarb, Inc. Immunogenic shiga-like toxin II variant mutants
US5821052A (en) * 1992-04-16 1998-10-13 University Of Medicine And Dentistry Of New Jersey Control of the synthesis of proteins by anitisense RNA-tRNA complex
WO1999027135A2 (fr) * 1997-11-21 1999-06-03 Yale University Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552144A (en) * 1992-01-22 1996-09-03 Microcarb, Inc. Immunogenic shiga-like toxin II variant mutants
US5821052A (en) * 1992-04-16 1998-10-13 University Of Medicine And Dentistry Of New Jersey Control of the synthesis of proteins by anitisense RNA-tRNA complex
CA2078716A1 (fr) * 1992-09-21 1994-03-22 Joyce De Azavedo Proteine de e. coli enterohemorragique de fixation et de destruction
WO1999027135A2 (fr) * 1997-11-21 1999-06-03 Yale University Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BARKER ROBERT H JR ET AL: "Inhibition of Plasmodium falciparum malaria using antisense oligodeoxynucleotides.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 1, 1996, 1996, pages 514 - 518, XP002167662, ISSN: 0027-8424 *
HARTH G ET AL: "Treatment of Mycobacterium tuberculosis with antisense oligonucleotides to glutamine synthetase mRNA inhibits glutamine synthetase activity, formation of the poly-L- glutamate/glutamine cell wall structure, and bacterial replication.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (2000 JAN 4) 97 (1) 418-23., XP002167663 *

Also Published As

Publication number Publication date
GB9929744D0 (en) 2000-02-09
WO2001044456A2 (fr) 2001-06-21
AU2196601A (en) 2001-06-25

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