WO2001038580A3 - Nucleic acid probe arrays - Google Patents

Nucleic acid probe arrays Download PDF

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Publication number
WO2001038580A3
WO2001038580A3 PCT/US2000/032131 US0032131W WO0138580A3 WO 2001038580 A3 WO2001038580 A3 WO 2001038580A3 US 0032131 W US0032131 W US 0032131W WO 0138580 A3 WO0138580 A3 WO 0138580A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
anchor primer
array
target nucleic
acid
Prior art date
Application number
PCT/US2000/032131
Other languages
French (fr)
Other versions
WO2001038580A2 (en
WO2001038580A9 (en
Inventor
Jonathan M Rothberg
Joel S Bader
Original Assignee
Curagen Corp
Jonathan M Rothberg
Joel S Bader
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Curagen Corp, Jonathan M Rothberg, Joel S Bader filed Critical Curagen Corp
Priority to CA002392474A priority Critical patent/CA2392474A1/en
Priority to JP2001539921A priority patent/JP2003515149A/en
Priority to AU17927/01A priority patent/AU783841B2/en
Priority to EP00980700A priority patent/EP1234058A2/en
Publication of WO2001038580A2 publication Critical patent/WO2001038580A2/en
Publication of WO2001038580A3 publication Critical patent/WO2001038580A3/en
Publication of WO2001038580A9 publication Critical patent/WO2001038580A9/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Disclosed are nucleic acid probe arrays and methods of identifying and sequencing nucleic acids in a population of nucleic acids using the arrays. The method is preferably performed by annealing a nucleic acid template to an anchor primer attached to a durface of the array. At least one anchor in the array has a sequence complementary to sequences at the 5' and 3' termini of a target nucleic acid.The annealed linear target nucleic acid is circularized using one or two ligation reactions. In one embodiment, one liggation is issued. Annealing of of the linear nucleic acid results in juxtaposition of the 5' and 3' termini of the target nucleic acid on the anchor primer. Addition of a ligase results in circulization of the target nucleic acid. This circularized nucleic acid is a template for extension of the anchor primer in a rolling circle amplification reaction. An extended anchor primer containing multiple copies of a sequence complementary to the circular mucleic acid is also referred to herein as a anchor primer nucleid acid-nucleic acid concatamer. The presence of multiple copies of the complementary sequence facilitates detection of the nucleic acid. Thus, the method provides for a highly sensitive method of detecting a desired nucleic acid attached at a discrete location on the array.
PCT/US2000/032131 1999-11-26 2000-11-27 Nucleic acid probe arrays WO2001038580A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002392474A CA2392474A1 (en) 1999-11-26 2000-11-27 Nucleic acid probe arrays
JP2001539921A JP2003515149A (en) 1999-11-26 2000-11-27 Nucleic acid probe array
AU17927/01A AU783841B2 (en) 1999-11-26 2000-11-27 Nucleic acid probe arrays
EP00980700A EP1234058A2 (en) 1999-11-26 2000-11-27 Nucleic acid probe arrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44940299A 1999-11-26 1999-11-26
US09/449,402 1999-11-26

Publications (3)

Publication Number Publication Date
WO2001038580A2 WO2001038580A2 (en) 2001-05-31
WO2001038580A3 true WO2001038580A3 (en) 2002-04-25
WO2001038580A9 WO2001038580A9 (en) 2002-12-05

Family

ID=23784034

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/032131 WO2001038580A2 (en) 1999-11-26 2000-11-27 Nucleic acid probe arrays

Country Status (5)

Country Link
EP (1) EP1234058A2 (en)
JP (1) JP2003515149A (en)
AU (1) AU783841B2 (en)
CA (1) CA2392474A1 (en)
WO (1) WO2001038580A2 (en)

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EP1598427A1 (en) * 2004-05-17 2005-11-23 Charité - Universitätsmedizin Berlin Method for the amplification of a target nucleic acid
EP2471921A1 (en) 2004-05-28 2012-07-04 Asuragen, Inc. Methods and compositions involving microRNA
EP2302054B1 (en) 2004-11-12 2014-07-16 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
SG162795A1 (en) 2005-06-15 2010-07-29 Callida Genomics Inc Single molecule arrays for genetic and chemical analysis
EP1762627A1 (en) 2005-09-09 2007-03-14 Qiagen GmbH Method for the activation of a nucleic acid for performing a polymerase reaction
US11306351B2 (en) 2005-12-21 2022-04-19 Affymetrix, Inc. Methods for genotyping
CA2663962A1 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir-31,mir-145, mir-147, mir-188, mir-215, mir-216, mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
WO2008036765A2 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Micrornas differentially expressed in pancreatic diseases and uses thereof
JP5719591B2 (en) 2007-06-08 2015-05-20 バイオジェン アイデック エムエー インコーポレイティドＢｉｏｇｅｎ Ｉｄｅｃ Ｉｎｃ． Biomarker for predicting anti-TNF responsiveness or non-responsiveness
JP5020734B2 (en) * 2007-07-31 2012-09-05 株式会社日立ハイテクノロジーズ Nucleic acid analysis method and apparatus
WO2009137807A2 (en) 2008-05-08 2009-11-12 Asuragen, Inc. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
US8486630B2 (en) * 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
GB0921264D0 (en) 2009-12-03 2010-01-20 Olink Genomics Ab Method for amplification of target nucleic acid
US10273544B2 (en) 2010-02-11 2019-04-30 Dana-Farber Cancer Institute, Inc. Methods for predicting likelihood of responding to treatment
WO2011108930A1 (en) 2010-03-04 2011-09-09 Interna Technologies Bv A MiRNA MOLECULE DEFINED BY ITS SOURCE AND ITS DIAGNOSTIC AND THERAPEUTIC USES IN DISEASES OR CONDITIONS ASSOCIATED WITH EMT
NZ719520A (en) 2010-07-06 2017-07-28 Int Tech Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
ES2629890T3 (en) 2010-11-17 2017-08-16 Interpace Diagnostics, Llc miRNA as biomarkers to distinguish between benign and malignant thyroid neoplasms
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
WO2012158238A2 (en) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Anti-müllerian hormone changes in pregnancy and prediction ofadverse pregnancy outcomes and gender
CN102732598B (en) * 2011-04-11 2017-03-01 陈先锋 A kind of complete genome DNA sequence assembly sequence measurement
EP2718866B1 (en) 2011-06-07 2020-09-09 Koninklijke Philips N.V. Providing nucleotide sequence data
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
WO2013063519A1 (en) 2011-10-26 2013-05-02 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
US20130142728A1 (en) 2011-10-27 2013-06-06 Asuragen, Inc. Mirnas as diagnostic biomarkers to distinguish benign from malignant thyroid tumors
EP2870263A1 (en) 2012-07-03 2015-05-13 InteRNA Technologies B.V. Diagnostic portfolio and its uses
US20140100124A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
KR101984700B1 (en) * 2012-11-19 2019-05-31 삼성전자주식회사 Polynucleotide and use thereof
EP2970909A4 (en) 2013-03-15 2017-02-15 The University of Chicago Methods and compositions related to t-cell activity
WO2014151551A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
EP2971132B1 (en) 2013-03-15 2020-05-06 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
US11859246B2 (en) * 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3495506B1 (en) 2013-12-11 2023-07-12 AccuraGen Holdings Limited Methods for detecting rare sequence variants
WO2016156576A1 (en) 2015-04-02 2016-10-06 Hmnc Value Gmbh Method of treatment using genetic predictors of a response to treatment with crhr1 antagonists
CN108368545B (en) 2015-10-09 2022-05-17 安可济控股有限公司 Methods and compositions for enriching amplification products
WO2017135383A1 (en) * 2016-02-03 2017-08-10 国立大学法人豊橋技術科学大学 Pseudo-circular double-stranded polynucleotide, and gene expression inhibitor using same
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Non-Patent Citations (3)

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Also Published As

Publication number Publication date
WO2001038580A2 (en) 2001-05-31
WO2001038580A9 (en) 2002-12-05
AU783841B2 (en) 2005-12-15
JP2003515149A (en) 2003-04-22
AU1792701A (en) 2001-06-04
EP1234058A2 (en) 2002-08-28
CA2392474A1 (en) 2001-05-31

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