WO2001036687A3 - Simultaneous hybridization/priming for snp analysis - Google Patents

Simultaneous hybridization/priming for snp analysis Download PDF

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Publication number
WO2001036687A3
WO2001036687A3 PCT/US2000/042117 US0042117W WO0136687A3 WO 2001036687 A3 WO2001036687 A3 WO 2001036687A3 US 0042117 W US0042117 W US 0042117W WO 0136687 A3 WO0136687 A3 WO 0136687A3
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WO
WIPO (PCT)
Prior art keywords
region
oligonucleotide
nucleotide
polymerase
variant
Prior art date
Application number
PCT/US2000/042117
Other languages
French (fr)
Other versions
WO2001036687A2 (en
Inventor
Ness Jeffrey Van
Original Assignee
Qiagen Genomics Inc
Ness Jeffrey Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Genomics Inc, Ness Jeffrey Van filed Critical Qiagen Genomics Inc
Priority to AU32694/01A priority Critical patent/AU3269401A/en
Publication of WO2001036687A2 publication Critical patent/WO2001036687A2/en
Publication of WO2001036687A3 publication Critical patent/WO2001036687A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The detection of variant nucleotides in a nucleic acid template may be achieved by contacting the template with a first oligonucleotide, a second oligonucleotide and a third oligonucleotide. The nucleic acid template has first, second and third regions. The first region is adjacent to and in the 3' direction from the second region, and the first oligonucleotide is substantially complementary to the first region and has a 3' end that is not extendible by a polymerase; the second region is between and adjacent to the first and third regions, and contains a site to be tested for the presence or absence of a variant nucleotide, the second region and second oligonucleotide having an identical number of nucleotides, the second oligonucleotide having a 3' end that is not extendible by a polymerase, and a nucleotide sequence such that (a) the second oligonucleotide hybridizes to the second region when the variant nucleotide in the second region is complementary to the corresponding nucleotide in the second oligonucleotide; and (b) the second oligonucleotide does not hybridize to the second region when the variant nucleotide in the second region is not complementary to the corresponding nucleotide in the second oligonucleotide; and the third region is adjacent to and in the 5' direction from the second region, the third oligonucleotide being substantially complementary to the third region and having a 3' end that is extendible by a polymerase. The template nucleic acid is contacted with the three oligonucleotides under reaction conditions where the 3' end of the third oligonucleotide undergoes an extension by polymerase depending on whether the second region contains the variant nucleotide.
PCT/US2000/042117 1999-11-15 2000-11-13 Simultaneous hybridization/priming for snp analysis WO2001036687A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU32694/01A AU3269401A (en) 1999-11-15 2000-11-13 Simultaneous hybridization/priming for snp analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16555099P 1999-11-15 1999-11-15
US60/165,550 1999-11-15

Publications (2)

Publication Number Publication Date
WO2001036687A2 WO2001036687A2 (en) 2001-05-25
WO2001036687A3 true WO2001036687A3 (en) 2002-06-06

Family

ID=22599390

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/042117 WO2001036687A2 (en) 1999-11-15 2000-11-13 Simultaneous hybridization/priming for snp analysis

Country Status (2)

Country Link
AU (1) AU3269401A (en)
WO (1) WO2001036687A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022175A1 (en) * 2001-07-26 2003-01-30 Harn-Jing Terng Diagnostic assay of genetic mutations by discriminating amplification and hybridization
EP1903117A1 (en) * 2006-09-22 2008-03-26 Veterinärmedizinische Universität Wien Methods for the detection of mutations by means of primers that hybridize contiguously
DK2588144T3 (en) 2010-07-02 2018-07-23 Ventana Med Syst Inc Detection of targets using mass markers and mass spectrometry
ITUA20163413A1 (en) * 2016-05-13 2017-11-13 Fondazione St Italiano Tecnologia Amplification reaction of nucleic acids with cooperative primers.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5578458A (en) * 1988-03-18 1996-11-26 Baylor College Of Medicine Mutation detection by competitive oligonucleotide priming
US5627032A (en) * 1990-12-24 1997-05-06 Ulanovsky; Levy Composite primers for nucleic acids
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
WO1998038338A1 (en) * 1997-02-28 1998-09-03 Exact Laboratories, Inc. Nucleic acid analysis methods

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5578458A (en) * 1988-03-18 1996-11-26 Baylor College Of Medicine Mutation detection by competitive oligonucleotide priming
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5627032A (en) * 1990-12-24 1997-05-06 Ulanovsky; Levy Composite primers for nucleic acids
WO1998038338A1 (en) * 1997-02-28 1998-09-03 Exact Laboratories, Inc. Nucleic acid analysis methods

Also Published As

Publication number Publication date
AU3269401A (en) 2001-05-30
WO2001036687A2 (en) 2001-05-25

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