WO2001034831A3 - Determination of catalase activity - Google Patents

Determination of catalase activity Download PDF

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Publication number
WO2001034831A3
WO2001034831A3 PCT/GB2000/004305 GB0004305W WO0134831A3 WO 2001034831 A3 WO2001034831 A3 WO 2001034831A3 GB 0004305 W GB0004305 W GB 0004305W WO 0134831 A3 WO0134831 A3 WO 0134831A3
Authority
WO
WIPO (PCT)
Prior art keywords
determination
catalase activity
test sample
catalase
peroxidase
Prior art date
Application number
PCT/GB2000/004305
Other languages
French (fr)
Other versions
WO2001034831A2 (en
Inventor
Peter James O'brien
Mark Raymond Slaughter
Original Assignee
Smithkline Beecham Plc
Smithkline Beecham Corp
Brien Peter James O
Mark Raymond Slaughter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Beecham Plc, Smithkline Beecham Corp, Brien Peter James O, Mark Raymond Slaughter filed Critical Smithkline Beecham Plc
Priority to AU11658/01A priority Critical patent/AU1165801A/en
Publication of WO2001034831A2 publication Critical patent/WO2001034831A2/en
Publication of WO2001034831A3 publication Critical patent/WO2001034831A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/30—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving catalase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

An assay for catalase in a test sample comprising contacting the test sample with enzymatically produced H2O2, a peroxidase and a chromogenic dye; and measuring the change in color intensity.
PCT/GB2000/004305 1999-11-12 2000-11-10 Determination of catalase activity WO2001034831A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU11658/01A AU1165801A (en) 1999-11-12 2000-11-10 Novel process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9926908.6 1999-11-12
GBGB9926908.6A GB9926908D0 (en) 1999-11-12 1999-11-12 Novel process

Publications (2)

Publication Number Publication Date
WO2001034831A2 WO2001034831A2 (en) 2001-05-17
WO2001034831A3 true WO2001034831A3 (en) 2002-03-21

Family

ID=10864482

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004305 WO2001034831A2 (en) 1999-11-12 2000-11-10 Determination of catalase activity

Country Status (3)

Country Link
AU (1) AU1165801A (en)
GB (1) GB9926908D0 (en)
WO (1) WO2001034831A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD4205C1 (en) * 2011-12-26 2013-09-30 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Method for determining the activity of catalase
US10920263B2 (en) 2016-03-01 2021-02-16 Sani-Marc Inc. Methods, compositions and kits for determining cleanness of a surface
CN111537463A (en) * 2020-04-15 2020-08-14 广东省第二人民医院（广东省卫生应急医院） Method for quantitatively detecting uric acid in serum

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926732A (en) * 1973-02-16 1975-12-16 Alfa Laval Ab Method for assaying catalase in milk and other liquids
GB1445793A (en) * 1973-02-16 1976-08-11 Alfa Laval Ab Determination of catalase in a liquid
EP0488244A1 (en) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Method of enzymatically measuring hydrogen peroxide and reagent therefor
EP0504865A1 (en) * 1991-03-22 1992-09-23 Junichi Iwamura Measurement of catalase activity
WO1994003632A1 (en) * 1992-08-05 1994-02-17 University Of The West Of England, Bristol Detection of microorganisms

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926732A (en) * 1973-02-16 1975-12-16 Alfa Laval Ab Method for assaying catalase in milk and other liquids
GB1445793A (en) * 1973-02-16 1976-08-11 Alfa Laval Ab Determination of catalase in a liquid
EP0488244A1 (en) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Method of enzymatically measuring hydrogen peroxide and reagent therefor
EP0504865A1 (en) * 1991-03-22 1992-09-23 Junichi Iwamura Measurement of catalase activity
WO1994003632A1 (en) * 1992-08-05 1994-02-17 University Of The West Of England, Bristol Detection of microorganisms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SLAUGHTER MARK R ET AL: "Fully-automated spectrophotometric method for measurement of antioxidant activity of catalase.", CLINICAL BIOCHEMISTRY, vol. 33, no. 7, October 2000 (2000-10-01), pages 525 - 534, XP000992836, ISSN: 0009-9120 *
VAN LENTE F ET AL: "COUPLED-ENZYME DETERMINATION OF CATALASE ACTIVITY IN ERYTHROCYTES", CLINICAL CHEMISTRY, vol. 36, no. 7, 1990, pages 1339 - 1343, XP002165294, ISSN: 0009-9147 *

Also Published As

Publication number Publication date
GB9926908D0 (en) 2000-01-12
AU1165801A (en) 2001-06-06
WO2001034831A2 (en) 2001-05-17

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