WO2001034198A2 - Oncolytic combinations for the treatment of cancer - Google Patents

Abstract

Leukotriene (LTB4) antagonists enhance the effectiveness of 2',2'-difluoronucleoside anti-cancer agents.

Description

ONCOLYTIC COMBINATIONS FOR THE TREATMENT OF CANCER

CROSS REFERENCE

This application claims priority from United States Provisional Patent Application No. 60/164,900 filed 11 November 1999; the entire disclosure of which is incorporated herein by reference.

FIELD OF THE INVENTION

This invention relates to a method of treating cancer with radiation therapy. More specifically, it relates to the use of radiation therapy, in conjunction with leukotriene inhibitors and 2 ' , 2 ' -difluoronucleoside anti- cancer agents which enhance the effectiveness of the radiation therapy.

BACKGROUND OF THE INVENTION

Leukotriene (LTB4) is a proinflammatory lipid that has been implicated in the pathogenesis of psoriasis, arthritis, chronic lung diseases, acute respiratory distress syndrome, and shock .

U.S. Patent 5,543,428 discloses the role of leukotriene inhibitors and reversing multi-drug resistance in a multi- drug resistant tumors. U.S. Patent 5,910,505 discloses that leukotriene (LTB4) antagonists may be used for the treatment or inhibition of oral squamous cell carcinoma. These leukotriene (LTB4) antagonist are well known in the art, and are fully described in U.S. Patent 5,462,954, which is hereby specifically incorporated by reference for its disclosure of leukotriene inhibitors, the methods of preparation of specific leukotriene (LTB4) antagonist, and compounds or formulations of the leukotriene (LTB4) antagonist which may be administered to patients.

U.S. Patent 5,464,826 discloses 2', 2'- difluoronucleoside anti-cancer agents. Such molecules are also disclosed in U.S. Patent 4,808,614.

Several types of radiation are used in the treatment of cancer including X-rays, gamma rays, high energy electrons and high LET (Linear Energy Transfer) radiation, such as, protons, neutrons, and alpha particles. The ionizing radiation is employed by techniques well known to those skilled in the art. For example, X-rays and gamma rays are applied by external and/or interstitial means from linear accelerators or radioactive sources. High-energy electrons can be produced by linear accelerators and high LET radiation is also applied from radioactive sources implanted interstitially . The total dose of radiation employed by one skilled in the art ranges from 18 to 160 Gray (Gy) . (One Gray unit of measure is equal to 100 rads) This total dose of radiation is usually or frequently divided into 5 to 7 continuous weeks of therapy. Typically, one week of radiation is divided into 5 daily fractions. A daily fraction of radiation consists of a dose from 1.2 to 2.5 Gray. The total amount of radiation used in brachytherapy may be 160 Gy. The exact dosage of radiation is dependent on a variety of factors including but not limited to the volume of the cancerous tissue to be irradiated, normal tissue surrounding the cancerous tissue, age of the patient, medical history of the patient, and other clinical factors. (R. Arriagada, Hematology/Oncology Clinics of North America, Vol. 11, pgs. 461-472 (1997) and S. Hellman, Principles of Cancer Management: Radiation Therapy, in Cancer: Principles and Practice of Oncology, 5^th Ed., Lippincott Publishers, pgs. 307-332 (1997); the disclosure of which is herein incorporated by reference.

Whatever the type of radiation used, it is believed that all radiation acts against cancer by a similar mechanism. Cancer cells are dividing rapidly, and it is thought that radiation disrupts the DNA of the cancer cells. This creates problems with cell division, and eventually results in the death of the irradiated cancer cells. Radiation also affects the normal tissue, and can lead to the death of normal cells as well. Accordingly, it is highly desirable to minimize the dose of radiation, to which the patient is exposed, in order to provide a treatment which is effective against cancer cells, and at the same time does not cause excessive damage to normal tissues.

Oxygen can act as a potentiator of radiation. Many tumors have rather low levels of oxygen in the interior of the tumor. Often radiation is more effective if oxygen can be provided to the tumor cell. Other potentiators are hypoxic cell sesitizers, non-hypoxic cell sensitizers, and oxygen delivery agents . These potentiators produce enhancement ratios between 1 and 3. Certain oxygen delivery agents are taught in US patent 5,295,944. 2 ' , 2 ' -difluoronucleosides apparently kill cancer cells by interfering with a DNA synthesis (S-phase) . They also appear to block the progression of cells through the Gl/S- phase boundary.

A preferred 2 ' , 2 ' -difluoronucleoside is gemcitabine HCl, also known as 2 ' , 2 ' -difluoro-2 ' -deoxycytidine monochloride and as 2 ' -deoxy-2 ' , 2 ' -difluorocytidine monochloride, which has the following structural formula:

SUMMARY OF THE INVENTION

Suprisingly, leukotriene (LTB4) inhibitors in conjunction with 2 ' , 2 ' -difluoronucleosides enhance the effects of radiation therapy in the treatment of cancer.

Surprisingly, we have now found a method of treating a human patient suffering from cancer which comprises administering to said patient ionizing radiation in conjunction with an effective amount of both a leukotriene (LTB4) antagonist and a 2 ',2'- difluoronucleoside .

DETAILED DESCRIPTION OF THE INVENTION

I. Definitions:

The term, "Acidic Group" means an organic group which when attached as the "Z" substituent of formula (I) or the "Z2" substituent of formula (II) acts as a proton donor capable of hydrogen bonding. An illustrative acidic group is carboxyl.

The term, "Active Ingredient" refers both to certain 2 ', 2 ' -difluoronucleoside compounds and also leukotriene B4 antagonist compounds generically described by formula A as well as diphenyl leukotriene B4 antagonist compounds generically described by formula (I) and formula (II) or the list of specific diphenyl compounds disclosed, infra., as well as a combination of a 2 ', 2 ' -difluoronucleoside and a leukotriene B4 antagonist described by formula A or formula I or II, and the salts, solvates, and prodrugs of such compounds .

The term, "alkenyl" means a monovalent radical of the generic formula C_nH2_n such as ethenyl, n-propenyl , isopropeneyl, n-butenyl, isobutenyl, 2-butenyl, and 3-butenyl .

The term, "alkyl" by itself or as part of another substituent means, unless otherwise defined, a straight or branched chain monovalent hydrocarbon radical such as methyl, ethyl, n-propyl , isopropyl, n-butyl, tertiary butyl, sec-butyl, n-pentyl, and n-hexyl . The term, "alkaryl" means an aryl radical substituted with an alkyl or substituted aryl group, for example:

In the term, "C5-C20 alkaryl" the numerical subscripts refer to the total number of carbon atoms in the radical.

The term, "C5-C20 aralkyl" means an alkyl radical substituted with an aryl or substituted aryl group, for example:

In the term, "C5-C20 aralkyl" the numerical subscripts refer to the total number of carbon atoms in the radical .

The term, "carbocyclic group" refers to a five, six, seven, or eight membered saturated, unsaturated or aromatic ring containing only carbon and hydrogen (e.g., benzene, cyclohexene, cyclohexane, cyclopentane) .

The term, "cycloalkyl" means a carbocyclic non- aromatic monovalent radical such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl .

The term, "halo" means fluoro, chloro, bromo, or iodo. The term, "heterocyclic radical (s) " refers to a radical having a saturated, unsaturated or aromatic five membered substituted or unsubstituted ring containing from 1 to 4 hetero atoms .

The terms, "mammal" and "mammalian" include human.

The term, "N-sulfonamidyl" means the radical:

where R12 is

alkyl, aryl, C1-C6 alkyl substituted aryl, C -C20 alkaryl, or C5-C20 aralkyl.

The term, "substituted alkyl" means an alkyl group further substituted with one or more radical (s) selected from halo, C^-Cg alkyl, aryl, benzyl, C2-C alkenyl, C2-C5 alkynyl, C3-C8 cycloalkyl, C^-Cg alkoxy, C1-C haloalkyl (e.g., -CF₃).

The term, "substituted aryl" means an aryl group further substituted with one or more radical (s) selected from halo, C1-C alkyl, aryl, benzyl, C2-C alkenyl, C2-Cg alkynyl, C3-C8 cycloalkyl, C^-Cg alkoxy, C^-Cg haloalkyl (e.g., -CF₃).

The term, "tetrazolyl" refers to an acidic group represented by either of the formulae:

The term "therapeutically effective interval" is a period of time beginning when one of either (a) the 2',2'- difluoronuceoside anti-cancer agent (b) the LTB4 antagonist or (c) radiation treatment is administered to a mammal and ending at the limit of the anti-cancer beneficial effect in treating cancer of (a) , (b) or (c) . Typically, the anti- cancer agents and the leukotriene (LTB4) antagonist are administered within 24 hours of each other, more preferably within 4 hours and most preferably within 1 hour.

The phrase "therapeutically effective combination", used in the practice of this invention, means administration of both (a) the 2 ', 2 ' -difluoronuceoside anti-cancer agent and (b) the LTB4 antagonist, and or (c) radiation treatment either simultaneously or separately.

The anti cancer agents which may be used are compounds of the formula:

wherein : R2 is hydrogen or

R2 is a base defined by one of the formulae

X is N or C-R⁴

R3 is hydrogen, C1-C4 alkyl or

R⁴ is hydrogen, C1-C4 alkyl, amino, bromo, fluoro, chloro or iodo;

Each R5 independently is hydrogen or C1-C4 alkyl; and the pharmaceutically-acceptable salts thereof.

The following compounds may also be used

wherein :

R6 is hydrogen, C1-C4 alkyl;

R⁷ is a base of one of the formulae

X is N or C-R⁴ ;

R^ is hydrogen or C1-C4 alkyl;

R⁴ is hydrogen, C1-C4 alkyl; amino, bromo, fluoro, chloro and iodo; and the pharmaceutically-acceptable salts thereof; with the proviso that R^ and R^ may both be hydrogen only when X is N and

wherein:

R6 is hydrogen or C1-C4 alkyl;

These compounds are disclosed in US Patent 5,464,826 which is incorporated by reference herein for its disclosure of the methods of preparing these compounds, formulating these compounds, and the treatment of cancer using these compounds .

Alternatively the anti-cancer compounds can be described as compounds represented by the formula:

where :

R is hydrogen;

R² is a base defined by one of the formulae:

X is C-R\-

R³ is hydrogen;

R⁴ is hydrogen, Cι-C alkyl , bromo , fluoro , chloro or iodo ; and pharmaceutically acceptable salts thereof.

More preferably, the anti-cancer compounds are those wherein R2 is the base defined by the formula:

Even more anti-cancer agents are selected from the group consisting of the following compounds or a pharmaceutically acceptable salt therof :

(i) 1- (4-amino-2-oxo-lH-pyrimidin-l-yl) -2 ' -desoxy- 2 ' , 2 ' -difluororibose,

(ii) 1- (4-amino-2-oxo-lH-pyrimidin-l-yl) -2-desoxy- 2 ' ,2' -difluoroxylose,

(iii) 1- (2 , 4-dioxo-lH, 3H-pyrimidin-l-yl) -2-desoxy- 2 ', 2 ' -difluororibose, and

(iv) 1- (4-amino-5-methyl-2-oxo-lH-pyrimidin-l-yl) -2- desoxy-2 ' , 2 ' -difluororibose .

The most preferred compound is gemcitabine HCl which is a nucleoside analogue that exhibits antitumor activity. Gemcitabine HCl is 2 ' -deoxy-2 ' , 2 ' -difluorocytidine monohydrochloride (β-isomer) , also known as 2 ' , 2 ' -difluoro- 2 ' -deoxycytidine monohydrochloride, or also as 1- (4-amino-2- oxo-lH-pyrimidin-1-yl) -2-desoxy-2 ' , 2 ' -difluororibose. The structural formula is as follows:

The 2 ', 2 ' -difluoronucleoside anti-cancer agents are generally mixed with a carrier which may act as a diluent or excipient. The anti-cancer agents may be administered in the form of tablets, pills, powders lozenges, sachets, cachets, elixirs, suspensions, emulsion, solution, syrups or aerosols. Sterile injectable solutions may also be used.

The leukotriene (LTB4) antagonists useful in the present invention include those given in formula A.

Formula A or a pharmaceutically acceptable base addition salt thereof, wherein:

Rl' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2 ' -substituted phenyl; each R2 ' and R3 ' are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1_.-C4 alkoxy, (C1-C4 alkyl) - (0) g S-, trifluoromethyl, or di- (C1-C3 alkyl) amino;

X' is -0-, -S-, -C(=0), or -CH2-;

Y' is -0- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C≡C-;

Z' is a straight or branched chain C1_.-C10 alkylidenyl;

A' is a bond, -0-, -S-, -CH=CH-, or -CRaRi)- , where R_a and Rjb are each independently hydrogen, C1-C5 alkyl, or R7 ' - substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring;

R4 - is R5 or one of the following formulae:

where each R6 is independently -COOH, 5-tetrazolyl, -

C0N(R₉)₂, or -CONHS0 Rιo each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-R6, -T-G-Rβ, (C1-C4 alkyl) -T- (C1-C4 alkylidenyl) -0- , or hydroxy;

R8 is hydrogen or halo; each Rg is independently hydrogen, phenyl, or C1_.-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;

RlO is C1-C4 alkyl or phenyl;

Rll is R2, -W-R6, or -T-G-R6; each W is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0-, -NH- , -NHCO-, -C(=0)-, or (0)_g S-;

K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2; p is 0 or 1; and t is 0 or 1; provided when X is -0- or -S-, Y is not -0- ; provided when A is -O- or -S-, R4 is not Rζ; and provided W is not a bond when p is 0.

More preferred compounds of Formula A are those wherein R4 ' is selected from the following formulae:

An even more preferred compound is that wherein R4 ' is

Preferred compounds or pharmaceutically acceptable acid salt derivatives thereof are those wherein said compound selected from the group (A) to (KKKK) consisting of: A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4-

( 4-fluoropheny1 ) -5-hydroxyphenoxy) heptane;

B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (3 -fluorophenyl) -5-hydroxyphenoxy) heptane;

C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy)propoxy) -6- (4- dimethylaminocarbonylbutyloxy) phenyl ) propion ic acid;

D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; E) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- carboxybutyloxy) phenyl) propionic acid;

F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid;

G) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl)butyloxy) phenyl) ropionic acid; H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) -

5-hydroxyphenoxy) - (1- butenyl) ) phenyl )propionate;

I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl) ) phenyl) propionic acid; J) 3-(2-(4-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) butyl ) phenyl ) propionic acid;

K) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) -6- methoxyphenyl) propionic acid;

L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -

5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl ) propionate ;

M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid; N) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- butyloxy) phenyl) propionic acid;

0) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) - 6- ( 4- methylthiobutyloxy) phenyl) propionic acid;

P) 3- (2- (3- (2, 4-Di- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- carboxybutoxy) phenyl) propionic acid;

Q) 6-Methyl-6- (lH-tetrazol-5-yl) -11- (2-ethyl-4- (4-fluorophenyl) -5 -hydroxyphenoxy) undecane; R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ;

S) N-Methanesulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide; T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; U) 3- (2- (3- (2-Butyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

V) Ethyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionate ;

W) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) henyl ) propionic acid;

X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- (4- (methoxycarbonyl ) phenoxy) phenyl ) propionate; Y) 3- (2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- carboxyphenoxy) phenyl ) propionic acid;

Z) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- ( 4- carboxyphenoxy) henyl ) propionic acid;

AA) 3,3-Dimethyl-3-(2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) ropoxy) phenyl ) ropionic acid;

BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ;

CC) 2-Methyl-2- (lH-tetrazol-5-yl) -3-hydroxy-3- (2-(3-(2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane;

DD) 3- (2- (3- (2-Bromo-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; EE) 3- (2- (3-(2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; FF) Methyl 3- (2-hydroxy-3- ( 4- methoxycarbonylbutyl ) -6- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionate ;

GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- (4- carboxybutyl ) dihydrocoumarin; HH) 2-Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxy] phenol sodium salt;

II) 2- (4-Methylphenyl) -4-ethyl-5- [ 6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

JJ) 2- (3-Methylphenyl) -4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;

KK) 2- (2-Methylphenyl) -4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt; LL) 2- (4-Methoxyphenyl)-4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;

MM) 2- (3-Methoxyphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt ;

NN) 2- (4-Trifluoromethylphenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

PP) 3- (5- (6- ( 4-Pheny1-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3,4 -tetrahydronaphthalen-1 (2H) - one)propanoic acid;

QQ) 3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3, 4-tetrahydronaphthalen-l (2H) - one)propanoic acid;

RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl-2 , 3- dihydroinden-1 (2H) -one)propanoic acid;

SS) 3,3-Dimethyl-5- (3- (2-carboxyethyl) -4- (3- (4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl) -5-oxopentanoic acid;

TT) 7- [3- [ ( 5-Ethyl-2-hydroxy [1, 1 ' -biphenyl] -4- yl) oxy] propoxy] -3 , 4-dihydro-8-propyl-2H-l- benzopyran-2-carboxylic acid;

UU) 8-Propyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3, 4-dihydro-2H-l- benzopyran-2-carboxylic acid;

W) 2-[3-[3-[ (5-Ethyl-2-hydroxy [1,1' -biphenyl] - 4-yl) oxy] propoxy] -2-propylphenoxy]propanoic acid; WW) 2-(4-Chlorophenyl) -4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl ) heptyloxy] phenol monosodium salt;

XX) 2-(3,5-Dichlorophenyl)-4-ethyl-5- [ 6-methyl- 6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt;

YY) 3-[2-[3-[ ( 5-Ethyl-2-hydroxy [1,1' -biphenyl ]- 4-yl) oxy] propoxy] -1-dibenzofuran] propanoic acid disodium salt;

ZZ) 7-Carboxy-9-oxo-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;

AAA) 2- [2-Propyl-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid sodium salt hemihydrate; BBB) 3- [3- ( 2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl] -4- propanoic acid disodium salt monohydrate; CCC) 5-Ethyl-4- [3- [2-propyl-3- [2- (2H-tetrazol-5- yl ) phenoxy] phenoxy] propoxy] [1,1' -biphenyl] - 2-ol disodium salt sesquihydrate; DDD) 3-[4-[3-[ 3- (2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ]propanoic acid sodium salt hemihydrate ; EEE) 2-Fluoro-6-[2-propyl-3-[3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt; FFF) 2-[2-Propyl-3-[3-[2-ethyl-4-(4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt; GGG) 3- [4- [7-Carboxy-9-oxo-3-[3-[2-ethyl-4-(4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate ; HHH) 3-[4-[9-Oxo-3-[3-[2-ethyl-4-(4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid;

III) 3- [2- [1- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- ( 5-oxo-5- morpholinopentanamido) phenyl] propanoic acid;

JJJ) 2-Fluoro-6- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate;

KKK) 4-Fluoro-2- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;

LLL) 2- [2-Propyl-3- [5- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] pentoxy] phenoxy] benzoic acid; MMM) 2- [2-Propyl-3- [4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] butoxy] phenoxy] benzoic acid sesquihydrate; NNN) 2- [2- (2-Methylpropyl)-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; OOO) 2-[2-Butyl-3-[3-[2-ethyl-5-hydroxy-4-(4- fluorophenyl) phenoxy] propoxy] phenoxy] benzoic acid hydrate;

PPP) 2- [2- (Phenylmethyl) -3- [3- [2-ethyl-5-hydroxy- 4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;

QQQ) 2- [2-Propyl-3- [3- [2 -ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] phenoxy] phenylacetic acid;

RRR) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid;

SSS) 2- [ [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenyl ] methyl ] benzoic acid;

TTT) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid;

UUU) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfinyl ] benzoic acid;

WV) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenylsulfonyl] benzoic acid hydrate;

WWW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- ( 4-fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenyl ] -4-pentynoic acid disodium salt 0.4 hydrate;

XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- ( 4-fluorophenyl ) -5-hydroxyphenoxy) hexane ;

YYY) 1- (4- (Carboxymethoxy) phenyl) -1- (lH-tetrazol- 5-yl)-6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane ;

ZZZ) 1- (4- (Dimethylammocarbonylmethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane; AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;

BBBB) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) -2-methyl-E- propenoic acid;

CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl ) -1H- tetrazole;

DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- ( 4- carboxybutyloxy) phenyl) propionic acid;

EEEE) 5- [3- [4- (4-Fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-one; FFFF) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;

GGGG) 3- (3- {3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4- propylphenyl) propanoic acid sodium salt;

HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -3- propylphenyl) propanoic acid; IIII) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl) propanoic acid;

JJJJ) 3-{3-[3-(2-Ethyl-5- hydroxyphenyloxy) propoxy] -2- propylphenyl}propanoic acid disodium salt; and

KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] benzoyl] benzoic acid disodium salt hemihydrate.

These leukotriene (LTB4) antagonists are well known in the art, and are fully described in U.S. Patent 5,462,954, which is hereby specifically incorporated by reference for its disclosure of the methods of preparation of specific leukotriene B antagonists and compounds or formulations of the leukotriene antagonists which may be administered to patients. A preferred compound is 2- [2-propyl-3- [3- [2- ethyl-5-hydroxy-4- (4-flourophenyl ) phenoxy] propoxy] phenoxy benzoic acid which can also be named 2- [3- [3- (5-ethyl-4 ' - flouro-2-hydroxybiphen-4-yloxy)propoxy-2- propylphenoxy] benzoic acid, described in U.S. Patent 5,462,954 as example 66 and also shown below as Compound A (Formula B) :

Compound A (Formula B) A second class of LTB4 antagonists to use as the essential co-agent in the compositions and practice of the method of this invention are those disclosed in copending provisional patent application, titled, "Heterocycle Substituted Diphenyl Leukotriene Antagonists" (inventor, Jason Scott Sawyer) containing 97 pages and identified as Eli Lilly and Company Docket No. B-13240), filed on November 11, 1999, and now Provisional patent Application Serial Number 60/164,786. This second class of heterocycle substituted diphenyl leukotriene antagonists are described in more detail below:

II. Additional LTB4 Antagonists:

Additional LTB4 antagonists are described below which are novel heterocyclic substituted diphenyl compounds of formula (I)

wherein:

X is selected from the group consisting of,

(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; or (ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i) ;

Yl is a bond or divalent linking group containing 1 to 9 atoms ;

Y2 and Y3 are divalent linking groups independently selected from -CH2-, -0-, and -S-;

Z is an Acidic Group;

Rl is C1-C10 alkyl, aryl, C3-C 0 cycloalkyl, C2-C 0 alkenyl, C2-C10 alkynyl, C5-C20 aralkyl, Cg-C20 alkaryl, Cχ-CiQ haloalkyl, C5-C20 aryloxy, or CI-C Q alkoxy;

R2 is hydrogen, halogen, CI-CI_Q haloalkyl, CI-CIQ alkoxy, ^cl"^c10 alkyl, C3-C8 cycloalkyl, Acidic Group, or - (CH2) 1-7 (Acidic Group);

R3 is hydrogen, halogen, C -CI_Q alkyl, aryl, CI~CIQ haloalkyl, CI-CI_Q alkoxy, CI~CIQ aryloxy, C3-C8 cycloalkyl;

R4 is C1-C4 alkyl, C3-C4 cycloalkyl,

- (CH2) 1-7 (cycloalkyl) , C2-C4 alkenyl, C2-C4 alkynyl, benzyl, or aryl ; and

n is O, 1, 2, 3, 4, 5, or 6;

or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof. III. Preferred LTB4 Antagonists include the following:

III A. Preferred X substituents:

A "substituted heterocyclic radical" is preferably Substitued with from 1 to 3 groups independently selected from hydrogen, halo, C -CI_Q alkyl, CI-CI_Q haloalkyl, C -C Q alkoxy, aryl, or C -C20 aryloxy. Preferred Group 1 of X substituent (symbol, "PG1-X")

Preferred LTB4 antagonist compounds of the invention include those wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following structural formulae:

where RIO is a radical selected from hydrogen or C1-C4 alkyl; and Rll is a radical selected from hydrogen, halo, CI-CIQ alkyl, C -CIQ haloalkyl, CI~C Q alkoxy, aryl, or C5-C20 aryloxy. Preferred RIO groups are hydrogen, methyl, or phenyl. Moreover, any of the above heterocyclic radicals illustrated by structural formulae may attach to the diphenyl leukotriene antagonist of formulae (I) by any monovalent bond originating on a suitable carbon or nitrogen atom in its ring structure.

For example, the pyrrole radical may attach to the diphenyl molecule by a single bond originating at any carbon atom or any nitrogen atom which has less than three bonds in the hererocyclic ring;

Location of attachment bond for pyrrole,

A preferred form of the substituent X is a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, for example:

III B. Preferred Group 2 of X substituent (symbol, "PG2- X") :

Most preferred as the X substituents are the heterocyclic radicals;

or

III C. Excluded X substituents: The heterocyclic radical X of Formula (I) does not include 3-bromo-l, 2 , 4 thiadiazole since the LTB4 antagonist activity of compounds containing this radical is considered too low to be an aspect of this invention.

Ill D. Preferred Yi substituents: Yi is a bond or divalent linking group containing 1 to 9 atoms independently selected from carbon, hydrogen, sulfur, nitrogen, and oxygen;

Preferred Group 1 of Yi substituent (symbol, "PGl-Yi") Preferred LTB4 antagonist compounds of the invention include those wherein Yi is a divalent linking group selected from the group consisting of substituents represented by the following formulae:

-C- -SO; H,

C — — C

H₂ H₂ u *->

H₂

H₂

H,

and

where R13 is hydrogen, methyl, or ethyl;

The above divalent groups may be used in their forward or reversed positions. For example, the group;

may be positioned as either,

in the displayed fragment of formula (I) .

III E. Preferred Group 2 of Yi substituent (symbol, "PG2-

Yi") :

The most preferred divalent Yi substituent is the group;

-O-

III F. Preferred Group 1 of Y₂ substituent (symbol, "PG1- Y₂") and Preferred Group 1 of Y₃ substituent (symbol, "PG1- Y₃"): The Y2 and Y3 substituents are preferably selected from -S- and -0- .

Ill G. Preferred Group 2 of Y₂ substituent (symbol, "PG2- Y₂") and Preferred Group 2 of Y₃ substituent (symbol, "PG2- Y₃" ) :

Most preferably both Y2 and Y3 are the group;

-o-

III H. Preferred Group 1 of Z substituent (symbol, "PG1-Z") :

Z is the Acidic Group as previously defined, Preferred is an acidic group selected from the following:

tetrazolyl,

-SO3H,

O

-OH or

where R12 is CI-C Q alkyl, aryl, C5-C20 alkaryl, or C5-C20 aralkyl. Preferred R12 groups are represented by the formulae:

III I. Preferred Group 2 of Z substituent (symbol, "PG2-Z") :

Highly preferred are the acidic groups; -5- tetrazolyl,

N-acyl sulfonamide, -SO3H, and carboxyl.

III J. Preferred Group 3 of Z substituent (symbol, "PG3-Z") : Carboxyl is the most preferred Z substituent.

Ill K. Preferred Group 1 of n subscript variable (symbol, "PGl-n")

The most preferred integer values for the divalent linking group, -(CH2)_n- , are n=l, n=2 , and n=3.

Ill L. Preferred Group 2 of n subscript variable (symbol, "PG2-n")

The most preferred integer value of n for the divalent linking group, -(CH2)_n- is n = 1.

Ill M. Preferred Group 1 of Rl substituent (symbol, "PG1- Rl") :

A preferred Rl group is methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, and 2-propenyl; with n- propyl being most preferred. III N. Preferred Group 1 of R2 substituent (symbol, "PG1-R2")and Preferred Group 1 of R3 substituent (symbol, "PG1-R3") : Preferred R2 and R3 groups are those wherein R2 and R3 are independently selected from hydrogen or methyl, ethyl, methoxy, ethoxy, halo, or -CF3 ; with R2 and R3 both being hydrogen as most preferred.

III 0. Preferred Group 1 of R4 substituent (symbol, "PG1-R4":)

Preferred R4 substituents are ethyl, propyl, and isopropyl .

III P. Combinations of substituents of the compound of Formula (I) :

The substituents of formula (I) are defined as "Z", "X" , "n" , "Rl " , "R2 " , "R3 " , "R4 " , "Yl " , "Y2 " , and "Y3 " . Moreover, as described in the preceding section, within each of the defined substituents of Formula (I) are

"preferred" and "most preferred" subgroups which define the variety of substituents to be used in the definition of LTB4 antagonists of the invention. These preferred subgroups are defined by designations such as "PG1-R4" as recited above. It is often advantageous to use combinations of preferred groups or combinations of preferred groups together with the general definition of variables given in Formula (I) . Suitable combinations of substituents are shown in the following three Tables (viz., R-Table, Y-Table & XZn-Table) . The following R-Table is used to select combinations of general and preferred groupings of the variables Rl, R2 , R3 and R4 for substitution in formula (I), as follows

R-Table

Thus, for example, the substituent combination, "R14" describes a substituent combinatorial choice for Formula (I) wherein Rl is selected from the preferred set of variables, "PG1-R1", that is, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, and 2-propenyl; the R2 substituent is selected from the preferred set of variables, "PG1-R2", that is, hydrogen or methyl, ethyl, methoxy, ethoxy, halo, or -CF3 ; the variable R3 has the scope defined in the generic formula (I), and the substituents suitable for R4 are selected from the preferred group, "PG1-R4" having the preferred set of variables, ethyl, propyl , and isopropyl.

The following Y-Table is used to select broad and preferred groupings of the variables Yl, Y2 , and Y3 for substitution in formula (I), as follows:

Y-Table

The following XZn-Table is used to select broad and preferred groupings of the variables X, Z, and n for substitution in formula (I), as follows:

XZn-Table

How to Use the Tables :

Any of the individual 16 combinations of the R substituents depicted in the R-Table may be used in combination with any of the 27 individual combinations of Y substituents depicted in the Y-Table, which may be used with any of the 24 combinations of XZn substituents depicted in the XZn-Table. For example, the substituent combination choice "R07, Y21, XZn03" defines substituent set selections for a subset of formula (I) useful in the practice of the invention.

Ill Q. Additional preferred LTB4 antagonists include those described by formula (II) :

wherein;

X2 is a heterocyclic radical selected from,

or

R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and

R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.

Z2 is carboxyl, tetrazolyl, N-sulfonamidyl . Preferred Compounds of the Invention:

III R. Specific compounds preferred as LTB4 antagonists are represented by the following structural formulae:

(Ci:

(C2;

(C3)

(C4)

(C6) :

(C7)

(C8)

(C9) :

(CIO) :

(Cll)

(C12)

(C13!

(C14)

(C15)

(C16)

(C17) :

(C18)

(C19)

(c2o;

(C21)

(C23) :

and all acid, salt, solvate and prodrug derivatives thereof III S. Highly Preferred LTB4 antagonists include the following:

and all acid, salt, solvate and prodrug derivatives thereof

The salts of the above diphenyl LTB4 antagonists of the invention, represented by formulae (A), (I) and (II) and the specific compounds set out by structural formulae in sections IIIR and HIS herein, are an additional aspect of the invention. The LTB4 compounds of the invention possess an Acidic Group (s) and at these sites various salts may be formed which are more water soluble and/or physiologically suitable than the parent compound in its acid form. Representative pharmaceutically acceptable salts, include but are not limited to, the alkali and alkaline earth salts such as lithium, sodium, potassium, calcium, magnesium, aluminum and the like. Sodium salts are particularly preferred. Salts are conveniently prepared from the free acid by treating the acid form in solution with a base or by exposing the acid to an ion exchange resin. For example, the (Acidic Group) of the Z of Formula (I) may be selected as -CO2H and salts may be formed by reaction with appropriate bases (e.g., NaOH, KOH) to yield the corresponding sodium or potassium salt.

Included within the definition of pharmaceutically acceptable salts are the relatively non-toxic, inorganic and organic base addition salts of compounds of the present invention, for example, ammonium, quaternary ammonium, and amine cations, derived from nitrogenous bases of sufficient basicity to form salts with the LTB4 antagonist compounds of this invention (see, for example, S. M. Berge, et al . , "Pharmaceutical Salts," J. Phar . Sci., 66: 1-19 (1977)). Certain compounds of the invention may possess one or more chiral centers and may thus exist in optically active forms. All such stereoisomers as well as the mixtures thereof are intended to be included in the invention. If a particular stereoisomer is desired, it can be prepared by methods well known in the art, for example, by using stereospecific reactions with starting materials which contain the asymmetric centers and are already resolved or, alternatively, by methods which lead to mixtures of the stereoisomers and subsequent resolution by known methods. For example, a racemic mixture may be reacted with a single enantiomer of some other compound. This changes the racemic form into a mixture of diastereomers . Then, because the diastereomers have different melting points, different boiling points, and different solubilities, they can be separated by conventional means, such as crystallization. Prodrugs are derivatives of the compounds of Formulae (A), (I) and (II), supra., which have chemically or metabolically cleavable groups and become by hydrolysis or under physiological conditions the compounds of the invention which are pharmaceutically active in vivo. Derivatives of the compounds of this invention have activity in both their acid and base derivative forms, but the acid derivative form often offers advantages of solubility, tissue compatibility, or delayed release in a mammalian organism (see, Bundgard, H., Design of Prodrugs, pp. 7-9, 21-24, Elsevier, Amsterdam 1985). Prodrugs include acid derivatives well known to practitioners of the art, such as, for example, esters prepared by reaction of the parent acidic compound with a suitable alcohol, or amides prepared by reaction of the parent acid compound with a suitable amine. Simple aliphatic or aromatic esters derived from acidic groups pendent on the compounds of this invention are preferred prodrugs. In some cases it is desirable to prepare double ester type prodrugs such as (acyloxy) alkyl esters or ( (alkoxycarbonyl) oxy) alkyl esters. Particularly preferred esters as prodrugs are methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, morpholinoethyl, and N, N-diethylglycolamido . Esters of carboxylic acids are preferred prodrugs of the compounds of the invention (viz., the compounds of Formula A, Formula I, Formula II and the specific compounds set out in Section IIIR and HIS, herein) .

Methyl ester prodrugs may be prepared by reaction of the acid form of a compound of formula (I) in a medium such as methanol with an acid or base esterification catalyst (e.g., NaOH, H2SO4). Ethyl ester prodrugs are prepared in similar fashion using ethanol in place of methanol.

N,N-diethylglycolamido ester prodrugs may be prepared by reaction of the sodium salt of a compound of Formula (I) (in a medium such as dimethylformamide) with 2-chloro-N,N- diethylacetamide (available from Aldrich Chemical Co., Milwaukee, Wisconsin USA; Item No. 25,099-6).

Morpholinylethyl ester prodrugs may be prepared by reaction of the sodium salt of a compound of Formula (I) (in a medium such as dimethylformamide) 4- (2- chloroethyl)morpholine hydrochloride (available from Aldrich Chemical Co., Milwaukee, Wisconsin USA, Item No. C4, 220-3).

Preferred LTB4 antagonist compounds include the compounds of Formula A, Formula (I) , or Formula (II) or the specific compounds of sections IIIR and HIS shown above by structural formula; wherein the acid, salt and prodrug derivatives thereof are respectively selected from: carboxylic acid, sodium salt, and ester prodrug.

IV. Method of Making the Compounds of the Invention General reaction schemes (not represented to be specific Examples) applicable for synthesis of the LTB4 antagonist compounds represented by formula (I) are set out below. Numerous literature references and Chemical Abstract registry numbers (e.g., RN 152609-60-4) are supplied as additional aids for preparing reagents used in practicing the synthesis schemes of the invention. REACTION SCHEMES FOR MAKING THE COMPOUNDS OF THE INVENTION

The following scheme illustrates a process for making Example (1), a 4-substituted oxazole LTB4 receptor antagonist:

Scheme 1

benzyl bromide, Cs₂C0₃, DMF

known compound: RN# 156005-61-7

R. W. Harper et al., J. Med. Chem. 1994, 37(15), 2411

1

(38)

Known chloride (26) may be alkylated with benzyl bromide to provide chloride (28). Reaction with known ester (30), catalyzed by a suitable base, provides acetophenone (32) . Oxidation with bis (trifluoroacetoxy) iodobenzene gives alpha- hydroxy ketone (34) , that may be cyclized with triflic anhydride and formamide to give the 4-substituted oxazole (36) . Debenzylation with boron trifluoride etherate and ethanethiol gives oxazole (38), that is hydrolyzed and protonated to provide Example (1) .

Scheme 2 The following scheme illustrates a process for making Example (2), a 5 (4) -substituted imidazole LTB4 receptor antagonist: Scheme 2

(44)

The trimethylsilyl enol ether of acetophenone (32) is formed and treated with N-chlorosuccinimide followed by tetra-n- butylammonium fluoride to provide the chloroketone (40) . Treatment of (40) with 2-benzyl-2-thiopseudourea and base provides imidazole (42), that is treated with boron trifluoride etherate and ethanethiol to give imidazole (44). Hydrolysis and protonation provide Example (2) as the hydrochloride salt.

Scheme 3

The following scheme illustrates a process for making Example (3), a 4-substituted thiazole LTB4 receptor antagonist:

Scheme 3

thioformamide, MgC0₃ dioxane

(46)

(48)

Chloroketone (40) is treated with thioformamide and magnesium carbonate to give thiazole (46) , that is debenzylated with boron trifluoride etherate and ethanethiol giving thiazole (48) . Hydrolysis and protonation provides Example (3 ) .

Scheme 4 The following scheme illustrates a process for making Example (4), a 5 (3 ) -substituted pyrazole LTB4 receptor antagonist:

Scheme 4

(32)

Treatment of acetophenone (32) with N, N-dimethylformamide dimethyl acetal gives enone (50) , that may be hydrolyzed, protonated, and then heated with hydrazine hydrate to provide pyrazole (52). Debenzylation of the resulting pyrazole with boron trifluoride etherate and ethanethiol gives Example (4) .

Scheme 5 The following scheme illustrates a process for making Example (5), a 5-substituted isoxazole LTB4 receptor antagonist:

Scheme 5

(50)

(54)

Treatment of enone (50) with hydroxylamine provides isoxazole (54), that is debenzylated with boron trifluoride etherate and ethanethiol to give isoxazole (56). Hydrolysis and protonation provides Example (5) .

Scheme 6 The following scheme illustrates a process for making Example (6), a 5 (4) -substituted 1, 2 , 3-triazole LTB4 receptor antagonist :

Scheme 6

known compounds: RN 152609-60-4 152609-76-2

J. S. Sawyer et al. J. Med. Chem. 1995, 38, 4411

Known phenol (30) is alkylated with known chloride (58) to give aryl bromide (60) . Treatment of (60) with tri-n- butylethynyltin and a palladium catalyst gives alkyne (62). Heating (62) with trimethylsilyl azide provides triazole (64), that is debenzylated with boron trifluoride etherate and ethanethiol to give triazole (66) . Hydrolysis and protonation provides Example (6).

Scheme 7 The following scheme illustrates a process for making Example (7), a 1-substituted pyrrole LTB4 receptor antagonist:

Scheme 7

References for formation of 1-aryl substituted pyrroles: M. Mure and J. P. Klinman, J. Am. Chem. Soc. 1995, 117(34), 8698; Y. Lee et al. J. Am. Chem. Soc. 1996, 118(30), 7241 4-Ethylbenzene-1, 3-diol (68) is treated with potassium nitrosodisulfonate followed by 3-pyrroline and benzylbromide and a base to provide pyrrole (70) . Alkylation with 1- bromo-3-chloropropane gives chloride (72), that is used to alkylate phenol (30) to give pyrrole (74) . Debenzylation with boron trifluoride etherate and ethanethiol provides Example (7) .

Scheme 8

The following scheme illustrates a process for making Example (8), a 5-substituted 1, 2 , 4-thiadiazole LTB4 receptor antagonist :

Scheme 8

The palladium-catalyzed addition of 4 , 4, 5 , 5-tetramethyl- fl, 3 , 2 ] dioxaborolane to bromide (60) gives boronic ester (76) . The palladium-catalyzed addition of 3-bromo-5-chloro-

1, 2, 4-thiadiazole to (76) gives ester (78). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, gives Example (8) .

Scheme 9 The following scheme illustrates a process for making Example (9), a 2-substituted thiophene LTB4 receptor antagonist:

Scheme 9

The palladium-catalyzed addition of boronic ester (76) to 2- bromothiophene, followed by debenzylation with boron trifluoride etherate and ethanethiol, provides thiophene (80) . Hydrolysis and salt formation provides Example (9) . Scheme 10 The following scheme illustrates a process for making Example (10), a 4-substituted pyrazole LTB4 receptor antagonist:

SchemelO

(76)

(82)

The palladium-catalyzed addition of boronic ester (76) to 1- methyl-4-iodopyrazole provides pyrazole (82). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, provides Example (10).

Scheme 11 The following scheme illustrates a process for making Example (11), a 2-substituted thiazole LTB4 receptor antagonist:

Scheme 11

PdCI₂(dppf), CSaCO_g, toluene

The palladium-catalyzed addition of boronic ester (76) to 2- bromothizaole provides thiazole (84). Debenzylation with boron trifluoride etherate and ethanethiol gives thiazole (86) . Hydrolysis and protonation provides Example (11) .

Scheme 12 The following scheme illustrates a process for making Example (12), a 4-substituted isoxazole LTB4 receptor antagonist:

Scheme12

(88)

The palladium-catalyzed addition of boronic ester (76) to 3 , 5-dimethyl-4-iodoisoxazole provides oxazole (88). Debenzylation with trimethylsilyl iodide, followed by hydrolysis and salt formation, provides Example (12).

Scheme 13

The following scheme illustrates a process for making Example (13), a 2-substituted furan LTB4 receptor antagonist:

Scheme 13

BBr„ CHCl

Debenzylation of bromide (60) with boron tribromide provides phenol (90), that is treated with tert-butyldimethylsilyl chloride and imidazole to give silyl ether (92). The palladium-catalyzed addition of (92) to furan-2-boronic acid provides furan (94). Hydrolysis and salt formation gives Example (13) .

Scheme 14 The following scheme illustrates a process for making Example (14), a 3-substituted furan LTB4 receptor antagonist:

Scheme 14

Pd(PPh₃)₄, aq. Na₂C0₃, THF

(92)

The palladium-catalyzed addition of (92) to furan-3-boronic acid provides furan (96) . Hydrolysis and salt formation gives Example (14) . Scheme 15 The following scheme illustrates a process for making Example (15), a 3-substituted tetrahydrofuran LTB4 receptor antagonist:

Scheme15

(100)

The palladium-catalyzed addition of bromide (60) to furan-3- boronic acid provides furan (98) . Hydrogenation over a palladium catalyst gives tetrahydrofuran (100) . Hydrolysis and salt formation gives Example (15) .

Scheme 16

The following scheme illustrates a process for making Example (16), a 2-substituted pyrrolidine LTB4 receptor antagonist:

Scheme 16

(60)

(106)

The palladium-catalyzed addition of bromide (60) to N-boc pyrrole-2-boronic acid provides pyrrole (102). Hydrogenation over a palladium catalyst gives pyrrolidine (104) . Hydrolysis and salt formation gives pyrrolidine (106) . Treatment with hydrochloric acid provides Example (16) as the hydrochloride salt.

Scheme 17 The following scheme illustrates a process for making Example (17), a 3-substituted thiophene LTB4 receptor antagonist:

Scheme 17

(58) (108)

(112)

BBfø, CH CI

(114)

The palladium-catalyzed addition of bromide (58) to thiophene-3-boronic acid provides thiophene (108). Alkylation of known phenol (110) with (108) catalyzed by base provides thiophene (112). Debenzylation with boron tribromide gives thiophene (114) . Hydrolysis and protonation provide Example (17).

Scheme 18 The following scheme illustrates a process for making Example (18), a 5-substituted 1, 2 , 3 , 4-thiatriazole LTB4 receptor antagonist :

Scheme 18

2) Cs₂C0₃, BnBr, DMF

Reference for formation of dithioacids N C Gonnella et al Syn Commun 1979, 17

Reference for formation of 5-substιtuted 1 ,2,3,4-thιatrιazoles from dithioacids S I Ikeda et al , Synthesis 1990, 415 Phenol (30) is alkylated with l-bromo-3-chloropropane to give chloride (116) , that is in turn to be treated with known aldehyde (118) and a base, followed by benzylation with benzyl bromide and a base, to provide aldehyde (120) . From aldehyde (120) is made the thioacetal by treatment with 1, 2-ethanedithiol . The resulting thioacetal is then to be treated with base to provide the thioacid. Treatment with piperidine makes piperidinium salt (122). By the teaching of Ikeda, infra, (the disclosure of which is incorporated herein by reference) treatment of (122) with 2- chloropyridinium methyl iodide followed by azide ion will give the 1 , 2 , 3 , 4-thiatriazole (124). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (18) .

Scheme 19 The following scheme illustrates a process for making Example (19), a 4-substituted 1, 2 , 3-thiadiazole LTB4 receptor antagonist:

Scheme 19

NH,NHCOOEt

(130)

Reference for 1 ,2,3-thiadiazole formation: E. W. Thomas et al., J. Med. Chem. 1985, 28, 442. Treatment of acetophenone (32) with ethyl carbazate will give the hydrazone (128) . Use of thionyl chloride by the method of Thomas et . al . (infra., the disclosure of which is incorporated herein by reference) will give an intermediate 1, 2 , 3-thiadiazole (130), that is to be debenzylated with boron trifluoride etherate and ethanethiol, then hydrolyzed and protonated to give the product of Example (19) .

Scheme 20 The following scheme illustrates a process for making Example (20), a 3-substituted 1 , 2 , 5-thiadiazole LTB4 receptor antagonist :

Scheme 20

(62)

(132)

Reference for 1 ,2,5-thiadiazole formation: E. W. Thomas et al., J. Med. Chem. 1985, 28, 442.

Alkyne (62) is to be treated with trithiazyl trichloride by the method of Thomas et . al . (infra., the disclosure of which is incorporated herein by reference) to provide thiadiazole (132). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (20) . Scheme 21 The following scheme illustrates a process for making Example (21), a 2-substituted 1, 3 , 4-thiadiazole LTB4 receptor antagonist :

Scheme 21

(134)

The palladium-catalyzed addition of boronic ester (76) to 2- bromo-1, 3 , 4-thiadiazole will provide ester (134). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (21) .

Scheme 22 The following scheme illustrates a process for making Example (22), a 5-substituted isothiazole LTB4 receptor antagonist:

Scheme 22

(136)

The palladium-catalyzed addition of bromide (58) to 3- methylisothiazole-5-boronic acid will provide isothiazole (136) . Alkylation of phenol (30) with (136) catalyzed by base will provide isothiazole (138) . Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (22 ) .

Scheme 23 The following scheme illustrates a process for making Example (23), a 2-substituted oxazole LTB4 receptor antagonist:

Scheme 23

(140)

The palladium-catalyzed addition of boronic ester (76) to 2- bromooxazole will provide oxazole (140) . Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (23) . Scheme 24 The following scheme illustrates a process for making Example (24), a 3-substituted thiophane LTB4 receptor antagonist:

Scheme 24

Et₃SiH, TFA, benzene

(1 14)

(142)

Reference for formation of tetrahydrothiophenes: D. N. Kursanov et al. Tetrahedron 1975, 31, 311

Thiophene (114) may be reduced in the presence of triethylsilane and trifluoroacetic acid by the method of Kursanov et . al . (infra., the disclosure of which is incorporated herein by reference) to provide the thiophane (142). Hydrolysis and protonation will provide the product of Example (24) . V. PREPARATIVE EXAMPLES 1 TO 17:

Example 1

Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid.

known compound: R # 156005-61-7

R. W. Harper et al . , J. Med. Chem. 1994, 37 (15) , 2411-20

A. Preparation of 1- [2-benzyloxy-4- (3-c loropropoxy) -5- et ylphenyl] ethanone.

A mixture of 1- [2-hydroxy-4- (3-chloropropoxy) -5- ethylphenyl ] ethanone (26.1 g, 102 mmol), cesium carbonate (33.4 g, 103 mmol), and benzyl bromide (12.2 ml, 103 mmol), in N,N-dimethylformamide (300 mL) was stirred for 5 h at room temperature. The mixture was diluted with ethyl acetate and washed four times with water. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. The resulting oil was triturated with ethyl acetate and hexane, allowed to stand for 18 h, then cooled at 0 °C for 3 h. The resulting precipitate was collected via vacuum filtration to provide 24.3 g (69%) of the title compound as

1 white crystals: mp 60-61 °C. H NMR (CDCI3) 5 7.68 (s, 1H) , 7.40 (m, 5H) , 6.48 (s, IH) , 5.17 (s, 2H) , 4.13 (t, J = 6 Hz, 2H) , 3.75 (t, J = 6 Hz, 2H) , 2.56 (s, 3H) , 2.55

(q, J = 7 Hz, 2H) , 2.26 (quintet, J = 6 Hz, 2H) , 1.16 (t, J

+ = 7 Hz, 3H) ; TOF MS ES exact mass calculated for

C20H24CIO3 (p+1) : m/z = 347.1414. Found: 347.1402; IR

cm ) 1659, 1602, 1266

Anal. Calcd for C₂₀H₂₃Clθ3 : C, 69.26; H, 6.68. Found: C, 69.30; H, 6.52.

known compound: RN# 152609-76-2 J. S. Sawyer et al., J. Med. Chem.1995, 38, 4411

B. Preparation of 2-{3- [3- (4-acetyl-5-benzyloxy-2- ethylphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.

A mixture of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] ethanone (7.27 g, 21.0 mmol) and sodium iodide (3.14 g, 23.1 mmol) in 2-butanone (100 mL) was heated at reflux for 18 h. The mixture was cooled to room temperature, filtered, and concentrated in vacuo. The residue was dissolved in N,N-dimethylformamide (100 mL) and treated with 2- (3-hydroxy-2-propylphenoxy) benzoic acid methyl ester (6.0 g, 21 mmol) and potassium carbonate (3.2 g, 23 mmol) at room temperature for 15 h. The mixture was diluted with ethyl acetate and washed four times with water and once with saturated sodium chloride solution. The organic layer was dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 9.2 g

1 (72%) of the title compound as a colorless oil. H NMR

(CDC1₃) δ 7.88 (d, J = 9 Hz, IH) , 7.69 (s, IH) , 7.38 (m,

6H) , 7.12 (d, J = 8 Hz, IH) , 7.07 (d, J = 8 Hz, IH) , 6.80 (d, J = 8 Hz, IH) , 6.67 (d, J = 8 Hz, IH) , 6.50 (s, IH) , 6.44 (d, J = 9 Hz, IH) , 5.14 (s, 2H) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.65 (t, J = 7 Hz, 2H) , 2.57 (q, J = 7 Hz, 2H) , 2.56 (s, 3H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 7

Hz, 2H) , 1.15 (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; IR

-1 (CHCI3, cm ) 2965, 1726, 1602, 1461.

Anal. Calcd for C₃₇H₄₍₎0₇ : C, 74.48; H, 6.76. Found: C, 74.39; H, 6.77.

C. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (2- hydroxyacetyl)phenoxy]propoxy}-2-propylphenoxy)benzoic acid methyl ester.

A mixture of 2- {3- [3- (4-acetyl-5-benzyloxy-2- ethylphenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (5.31 g, 8.89 mmol) and water (10 mL) in acetonitrile (50 mL) was treated with trifluoroacetic acid (1.4 mL) , 18 mmol) and [bis (trifluoroacetoxy) iodo]benzene (7.65 g, 17.8 mmol) . The resulting mixture was heated at reflux for 4 h then concentrated in vacuo. The residue was dissolved in methylene chloride and washed once with water. The aqueous layer was extracted twice with fresh portions of methylene chloride. The combined organic layers were washed three times with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 20% ethyl acetate/80% hexane) of the residue provided

1 1.68 g (31%) of the title compound as a brown oil. H NMR

(CDC1₃) 5 7.92 (s, IH) , 7.88 (d, J = 9 Hz, IH) , 7.40 (m, 6H) , 7.12 (d, J = 9 Hz, IH) , 7.05 (d, J = 9 Hz, IH) , 6.79 (d, J = 8 Hz, IH) , 6.66 (d, J = 8 Hz, IH) , 6.50 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.15 (s, 2H) , 4.65 (s, 2H) , 4.22 (m, 4H) , 3.83 (s, 3H) , 2.65 (m, 4H) , 2.34 (quintet, J = 6 Hz,

2H) , 1.55 (hextet, J = 7 Hz, 2H) , 1.17 (t, J = 8 Hz, 3H) ,

+ 0.89 (t, J = 8 Hz, 3H) ; TOS MS ES exact mass calculated

for C₃₇H₄₁0₈ (p+1) : m/z = 613.2801. Found: 613.2833.

D. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-oxazol-4- yl-phenoxy)propoxy] -2-propylphenoxy} enzoic acid methyl ester.

To a solution of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (2- hydroxyacetyl ) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (1.39 g, 2.27 mmol) in methylene chloride (20 mL) cooled to -78 °C was added triflie anhydride (0.57 mL, 3.4 mmol) and 2,6-lutidine (0.40 mL, 3.4 mmol). The resulting mixture was stirred for 1 h then poured into ether and water. The organic layer was separated and washed once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in a 2:1 mixture of formamide/N,N- dimethylformamide (9 mL) and heated at 120 °C in a sealed tube for 4 h. The mixture was cooled to room temperature and diluted with ethyl acetate. The mixture was washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 89 mg (6%) of the title

1 product as a colorless oil. H NMR (CDCI3) δ 7.92 (s, IH) ,

7.85 (s, IH) , 7.83 (m, 2H) , 7.35 (m, 6H) , 7.03 (d, J = 8 Hz,

IH) , 7.00 (d, J = 8 Hz, IH) , 6.73 (d, J = 8 Hz, IH) , 6.62

(d, J = 8 Hz, IH) , 6.52 (s, IH) , 6.35 (d, J = 8 Hz, IH) ,

5.07 (s, 2H) , 4.14 (m, 4H) , 3.76 (s, 3H) , 2.61 (m, 4H) , 2.26

(quintet, J = 6 Hz, 2H) , 1.48 (hextet, J = 7 Hz, 2H) , 1.15

(t, J = 8 Hz, 3H) , 0.84 (t, J = 8 Hz, 3H) .

E. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester. To a solution of 2- (3- [3- (5-benzyloxy-2-ethyl-4-oxazol-4-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (89 mg, 0.14 mmol) in ethanethiol (2 mL) was treated with boron trifluoride etherate (0.27 mL, 2.2 mmol) at room temperature for 4 h. The solution was poured into ether and washed once with water, once with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided 34 mg (45%) of the title

1 product as a light brown oil. H NMR (CDCI3) δ 7.99 (d, J =

1 Hz, IH) , 7.90 (d, J = 1 Hz, IH) , 7.88 (dd, J = 8, 2 Hz,

IH) , 7.38 (t, J = 7 Hz, IH) , 7.15 (s, IH) , 7.10 (d, J = 9

Hz, IH) , 7.06 (d, J = 9 Hz, IH) , 6.81 (d, J = 9 Hz, IH) ,

6.70 (d, J = 9 Hz, IH) , 6.52 (s, IH) , 6.44 (d, J = 9 Hz,

IH) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.65 (t, J = 8 Hz, 2H) ,

2.58 (q, J = 8 Hz, 2H) , 2.33 (quintet, J = 6 Hz, 2H) , 1.55

(hextet, J = 7 Hz, 2H) , 1.17 (t, J = 8 Hz, 3H) , 0.91 (t, J =

8 Hz, 3H) ; MS ES+ m/e = 532 (p + 1).

F. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy)propoxy] -2-propylphenoxy} enzoic acid.

To a solution of 2- {3- [3- (2-ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (89 mg, 0.14 mmol) in methanol (2 mL) was added 1 M lithium hydroxide solution (0.28 mL) and the resulting mixture warmed at 60 °C for 3.5 h. The mixture was cooled to room temperature and concentrated in vacuo. The aqueous residue was diluted with water and the pH adjusted to ~4. The mixture was extracted three times with methylene chloride. The combined organic extracts were dried (sodium sulfate) , filtered, and concentrated in vacuo to provide 27 mg (92%)

1 of the title compound as a yellow solid. H NMR (DMSO-dg) δ 12.83 (bs, IH) , 10.12 (bs, IH) , 8.39 (s, IH) , 8.25 (s, IH) , 7.78 (dd, J = 8, 1 Hz, IH) , 7.64 (s, IH) , 7.47 (t, J = 8 Hz, IH) , 7.16 (m, 2H) , 6.80 (t, J = 8 Hz, 2H) , 6.56 (s, IH) , 6.35 (d, J = 8 Hz, IH) , 4.20 (t, J = 6 Hz, 2H) , 4.12 (t, J = 6 Hz, 2H) ; 2.54 (m, 4H) , 2.24 (quintet, J = 6 Hz,

2H) , 1.43 (hextet, J = 8 Hz, 2H) , 1.10 (t, J = 8 Hz, 3H) ,

+ 0.80 (t, J = 8 Hz, 3H) ; TOF MS ES exact mass calculated

for C₃₀H₃₂NO₇ (p+1) : m/z = 518.2179. Found: 518.2206; IR

-1 (KBr, cm ) 2961, 1696, 1460, 1222.

Anal. Calcd for C₃₀H₃₁NO₇: C, 69.62; H, 6.04; N, 2.71.

Found: C, 68.71; H, 5.82; N, 2.65. Example 2 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (3ff-imidazol-4- yl)phenoxy]propoxy} -2-propy1-phenoxy)benzoic acid hydrochloride.

A. Preparation of 2- (3- {3- [5-benzyloxy-4- (2-chloroacetyl) - 2-ethylphenoxy]propoxy} -2-propylphenoxy)benzoic acid methyl ester.

To a solution of 2- {3- [3- (4-acetyl-5-benzyloxy-2- ethylphenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (3.04 g, 5.09 mmol) in tetrahydrofuran (50 mL) cooled to -78 °C was added a solution of 1 M lithium hexamethyldisilazide in tetrahydrofuran (11.2 mL, 11.2 mmol) portion wise. After stirring for 20 min, trimethylsilyl chloride (2.6 mL, 20 mmol) was added and the mixture warmed to 0 °C and stirred for 30 min. The mixture was evaporated in vacuo and the residue dissolved in hexane. The resulting solution was filtered and concentrated in vacuo. The residue was dissolved in tetrahydrofuran (50 mL) , cooled to 0 °C, and treated with N-chlorosuccinimide (750 mg, 5.6 mmol) . The mixture was warmed to room temperature and stirred for 30 min, then heated at reflux for 2 h. The mixture was cooled to room temperature and treated with water (4 mL) and a solution of 1 N tetra-n-butylammonium fluoride in tetrahydrofuran (6 mL) . After stirring for 15 min the mixture was diluted in ether and washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 1.94 g (60%) of the title compound as a

1 white solid. H NMR (CDC1₃) δ 7.89 (d, J = 8 Hz , IH) , 7.77

(s, IH) , 7.40 (m, 6H) , 7.12 (d, J = 9 Hz, IH) , 7.06 (d, J = 8 Hz, IH) , 6.80 (d, J = 8 Hz, IH) , 6.66 (d, J = 8 Hz, IH) , 6.49 (s, IH) , 6.43 (d, J - 8 Hz, IH) , 5.15 (s, 2H) , 4.68 (s, 2H) , 4.20 (q, J = 6 Hz, 4H) , 3.82 (s, 3H) , 2.65 (t, J = 7 Hz, 2H) , 2.59 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz,

2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.16 (t, J = 8 Hz, 3H) ,

+ 0.89 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated

for C₃₇H₄₀ClO₇ (p+1) : m/z = 631.2463. Found: 631.2470; IR

-1 (CHCI3, cm ) 2964, 1720, 1603, 1461.

Anal. Calcd for C₃₇H₃₉C10₇ : C, 70.41; H, 6.23. Found: C, 70.04; H, 5.97.

B. Preparation of 2- (3-{3- [5-benzyloxy-4- (2-benzylsulfanyl- 3H-imidazol-4-yl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy)benzoic acid methyl ester.

A mixture of 2- (3- {3- [5-benzyloxy-4- (2-chloroacetyl) -2- ethylphenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (800 mg, 1.27 mmol), 2-benzyl-2-thiopseudourea hydrochloride (313 mg, 1.52 mmol), sodium iodide (77 mg, 0.51 mmol), and potassium carbonate (700 mg, 5.06 mmol) in N,N-dimethy1formamide (20 mL) was treated at 80 °C for 6 h. The mixture was cooled, diluted with diethyl ether, and washed once with water. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane) of the residue provided 376 mg (40%) of the title compound as a

1 yellow amorphous solid. H NMR (CDCI3) δ 7.89 (d, J = 8 Hz ,

IH) , 7.36 (m, 9H) , 7.20 (m, 5H) , 7.21 (d, J = 9 Hz, IH) , 7.06 (d, J = 8 Hz, IH) , 6.79 (d, J = 8 Hz, IH) , 6.67 (d, J = 8 Hz, IH) , 6.55 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.07 (s, 2H) , 4.21 (t, J = 6 Hz, 2H) , 4.18 (t, J = 6 Hz, 2H) , 4.10 (s, 2H) , 3.83 (s, 3H) , 2.63 (m, 4H) , 2.31 (quintet, J = 6

Hz, 2H) , 1.55 (hextet, J = 7 Hz, 2H) , 1.18 (t, J = 8 Hz,

+ 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass

calculated for C₄₅H₄₇N₂0₆S (p+1): m/z = 743.3155. Found:

-1 743.3142; IR (CHCI3, cm ) 2963, 1720, 1602, 1453.

Anal. Calcd for C₄₅H₄₆N₂0gS: C, 72.75; H, 6.24; N, 3.77.

Found: C, 72.69; H, 6.17; N, 3.56

C. Preparation of 2-(3-{3- [4- (2-benzylsulfanyl-3H-imidazol- 4-yl) -2-ethyl-5-hydroxyphenoxy]propoxy}-2- propylphenoxy)benzoic acid methyl ester.

A solution of 2- (3- {3- [5-benzyloxy-4- (2-benzylsulfanyl-3H- imidazol-4-yl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (360 mg, 0.49 mmol) in ethanethiol (7 mL) was treated with boron trifluoride etherate at room temperature for 3.5 h. The mixture was diluted with diethyl ether and water. The organic layer was separated and washed with saturated sodium bicarbonate solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 20% ethyl acetate/80% hexane) of the residue provided 154 mg (48%) of the title

1 compound as an orange oil. H NMR (CDCI3) δ 7.85 (d, J = 8 Hz, IH) , 7.36 (t, J = 7 Hz, IH) , 7.20 (m, 7H) , 7.12 (s, IH) , 7.05 (m, 3H) , 6.79 (d, J = 8 Hz, IH) , 6.65 (d, J = 8 Hz, IH) , 6.54 (s, IH) , 6.41 (d, J = 8 Hz, IH) , 4.20 (s, 2H) , 4.17 (m, 4H) , 3.82 (s, 3H) , 2.62 (t, J = 8 Hz, 2H) , 2.54 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.53 (hextet, J = 8 Hz, 2H) , 1.14 (t, J = 7 Hz, 3H) , 0.89 (t, J = 8 Hz, 3H) ;

+ TOF MS ES exact mass calculated for C₃₈H₄₁N₂0gS (p+1) : m/z = 653.2685. Found: 653.2669.

Anal. Calcd for C₃₈H₄₀N₂O₆S: C, 69.92; H, 6.18; N, 4.29.

Found: C, 69.44; H, 6.25; N, 3.99.

D. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (3H- imidazol-4-yl)phenoxy]propoxy} -2-propyl-phenoxy)benzoic acid hydrochloride.

A solution of 2- (3-{3- [4- (2-benzylsulfanyl-3H-imidazol-4- yl) -2-ethyl-5-hydroxyphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (154 mg, 0.235 mmol) in methanol (3 mL) was treated with 1 N lithium hydroxide solution at 60 °C for 3.5 h. The mixture was cooled to room temperature and concentrated in vacuo . The solution was diluted with water and adjusted to pH 4. The aqueous solution was extracted three times with methylene chloride. The combined organic layers were dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in ethanol (3 mL) and treated with 0.2 N sodium hydroxide solution (1 mL) and Raney nickel (75 mg) at 75 °C for 4 h. The mixture was cooled to room temperature, TM filtered through Celite , and the filtrate concentrated in vacuo. The residue was diluted with water and adjusted to pH 2 with 1 N hydrochloric acid. The resulting precipitate was collected via vacuum filtration to provide 27 mg (21%)

+ of the title compound. TOF MS ES exact mass calculated

for C3₀H₃₃N₂O₆ (p+1) : m/z = 517.2339. Found: 517.2340.

Example 3 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-thiazol-4-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid.

A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-thiazol-4- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A mixture of 2- (3- {3- [5-benzyloxy-4- (2-chloroacetyl) -2- ethylphenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (500 mg, 0.792 mmol), thioformamide (20 mL, 8.0 mmol), and magnesium carbonate in dioxane (10 mL) was heated at reflux for 2 h. The mixture was cooled to room temperature and diluted with diethyl ether and 0.2 M sodium hydroxide solution. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided

254 mg (50%) of the title compound as a colorless oil H

NMR (CDC1₃) δ 8.91 (s, IH) , 8.11 (s, IH) , 7.87 (dd, J = 8, 1

Hz, IH) , 7.84 (d, J = 1 Hz, IH) , 7.40 (m, 6H) , 7.08 (m, 2H) , 6.80 (d, J = 8 Hz, IH) , 6.68 (d, J = 8 Hz, IH) , 6.62 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.16 (s, 2H) , 4.21 (t, J = 6 Hz, 4H) , 3.83 (s, 3H) , 2.68 (m, 4H) , 2.32 (quintet, J = 6

Hz, 2H) , 1.56 (hextet, J = 8 Hz, 2H) , 1.21 (t, J = 7 Hz,

+ 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass

calculated for C₃₈H₄₀ O₆S (p+1) : m/z = 638.2576. Found:

-1 638.2579. IR (CHCI3, cm ) 2964, 1719, 1563, 1461.

B. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-4- yl-phenoxy)propoxy] -2-propylphenoxy} enzoic acid methyl ester.

A solution of 2-{3- [3- (5-benzyloxy-2-ethyl-4-thiazol-4-yl- phenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (243 mg, 0.366 mmol) in ethanethiol (7 mL) was treated with boron trifluoride etherate at room temperature for 4 h. The mixture was diluted with diethyl ether, washed once with water, once with saturated sodium bicarbonate solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided 131 mg (65%) of the title compound as a

1 colorless oil. H NMR (CDC1₃) δ 8.88 (d, J = 1 Hz , IH) ,

7.88 (dd, J = 8, 1 Hz, IH) , 7.44 (d, J = 1 Hz, IH) , 7.38 (m, 2H) , 7.08 (m, 2H) , 6.81 (d, J = 8 Hz, IH) , 6.68 (d, J = 8

Hz, IH) , 6.55 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 4.21 (t, J = 6 Hz, 4H) , 3.83 (s, 3H) , 2.63 (m, 4H) , 2.33 (quintet, J = 6

Hz, 2H) , 1.56 (hextet, J = 8 Hz, 2H) , 1.19 (t, J = 8 Hz,

+ 3H) , 0.91 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass

calculated for C₃₁H₃₄N0₆S (p+1) : m/z = 548.2107. Found: 548.2085.

C. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-4- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid.

A solution of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-4-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (130 mg, 0.236 mmol) in methanol (4 mL) was treated with 1 M lithium hydroxide solution at 60 °C for 3 h. The mixture was cooled to room temperature, concentrated in vacuo, and diluted with water. The solution was adjusted to pH ~4 and extracted three times with methylene chloride. The combined organic layers were dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in a minimum of methylene chloride and hexane was added until the solution became cloudy. The mixture was concentrated slowly in vacuo to give 96 mg (76%) of the title compound. H NMR

(CDC1₃) δ 8.90 (s, IH) , 8.23 (dd, J = 8, 1 Hz, IH) , 7.41 (m,

2H) , 7.38 (s, IH) , 7.29 (m, 2H) , 6.82 (d, J = 8 Hz, IH) , 6.71 (d, J = 8 Hz, IH) , 6.62 (d, J = 8 Hz, IH) , 6.54 (s, IH) , 4.25 (t, J = 6 Hz, 2H) , 4.22 (t, J = 6 Hz, 2H) , 2.59 (m, 4H) , 2.35 (quintet, J = 6 Hz, 2H) , 1.50 (hextet, J = 8

Hz, 2H) , 1.19 (t, J = 7 Hz, 3H) , 0.88 (t, J = 8 Hz, 3H) ;

+ TOF MS ES exact mass calculated for C3_QH₃₂N0₆S (p+1) : m/z

-1 = 534.1950. Found: 534.1957. IR (CHCI3, cm ) 2965, 1738,

1454.

Anal. Calcd for C₃₀H₃₁NO₆S: C, 67.52; H, 5.86; N, 2.62.

Found: C, 67.19; H, 5.72; N, 2.53.

Example 4 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (2ff-pyrazol-3- yl)phenoxy]propoxy} -2-propy1-phenoxy)benzoic acid.

A. Preparation of 2- (3-{3- [5-benzyloxy-4- (3- dimethylaminoacryloyl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy)benzoic acid methyl ester. A mixture of 2- (3- {3- [4-acetyl-5-benzyloxy-2- ethylphenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (3.07 g, 5.04 mmol) and dimethylformamide dimethylacetal (0.9 mL, 7 mmol) in N,N-dimethylformamide (3 mL) was heated at 110-120 °C for 35 h. The mixture was cooled to room temperature and diluted with a mixture of ethyl acetate and 1 N hydrochloric acid. The organic layer was separated, washed twice with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane to ethyl acetate) of the residue provided 2.1 g (63%) of the title compound as a yellow oil.

+ TOF MS ES exact mass calculated for C_4QH₄₆N0₇ (p+1) : m/z

-1 = 652.3274. Found: 652.3270. IR (CHCI3, cm ) 2965, 1720,

1605.

Anal. Calcd for C_4QH₄₅N0₇ : C, 73.71; H, 6.96; N, 2.15.

Found: C, 73.72; H, 6.95; N, 2.18.

B. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (2H- pyrazol-3-yl)phenoxy]propoxy} -2-propylphenoxy)benzoic acid.

A solution of 2- (3-{3- [5-benzyloxy-4- (3- dimethylaminoacryloyl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (550 mg, 0.843 mmol in methanol (30 mL) was treated with 1 M lithium hydroxide solution at 60 °C for 3 h. The mixture was cooled to room temperature and diluted with ethyl acetate and 0.5 M hydrochloric acid. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in methanol (15 mL) and treated with water (4 mL) and hydrazine monohydrate (0.50 mL, 7.7 mmol) at reflux for 3 h. The mixture was diluted with ethyl acetate and 1 N hydrochloric acid. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate), filtered and concentrated in vacuo. Chromatography (30% ethyl acetate/69% hexane/1% acetic acid) of the residue provided 350 mg (65%) of the title compound as the acetate salt. A portion of this material was free- based with sodium bicarbonate to provide an analytical

1 sample. H NMR (CDCl₃) δ 8.20 (dd, J = 8, 2 Hz, IH) , 7.55

(s, IH) , 7.44 (s, IH) , 7.38 (m, 5H) , 7.15 (m, 2H) , 6.78 (d, J = 8 Hz, IH) , 6.65 (d, J = 8 Hz, IH) , 6.61 (d, J = 8 Hz, IH) , 6.58 (s, IH) , 6.55 (bs, IH) , 5.18 (s, 2H) , 4.22 (t, J = 6 Hz, 2H) , 4.17 (t, J = 6 Hz, 2H) , 2.58 (m, 4H) , 2.30

(quintet, J = 6 Hz, 2H) , 1.47 (hextet, J = 8 Hz, 2H) , 1.18

+ (t, J = 7 Hz, 3H) , 0.88 (t, J = 8 Hz, 3H) ; TOF MS ES exact

mass calculated for C₃₇H_3gN₂0₆ (p+1) : m/z = 607.2808.

-1 Found: 607.2831. IR (CHCI3, cm ) 2965, 1739, 1604, 1454. Anal. Calcd for C₃₇H₃₈N₂0₆: C, 73.25; H, 6.31; N, 4.62 Found: C, 73.31; H, 6.30; N, 4.62.

C. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (2H-pyrazol- 3-yl)phenoxy]propoxy} -2-propylphenoxy)benzoic acid.

A solution of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (2f-pyrazol-3- yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid (300 mg, 0.490 mmol) in ethanethiol (2.5 mL) was treated with boron trifluoride etherate (2 mL) at room temperature for 3 h, at which time an additional portion of boron trifluoride etherate (1 mL) was added and stirring resumed for an additional 1 h. The mixture was diluted with diethyl ether and water. The organic layer was separated, washed with water, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane to 60% ethyl acetate/40% hexane) of the residue provided 60 mg (24%) of the title compound as a white solid.

1 H NMR (CDC1₃) δ 8.23 (d, J = 8 Hz, IH) , 7.61 (s, IH) , 7.42

(t, J = 7 Hz, IH) , 7.30 (s, IH) , 7.19 (d, J = 8 Hz, IH) , 7.15 (d, J = 8 Hz, IH) , 6.81 (d, J = 8 Hz, IH) , 6.69 (d, J 8 Hz, IH) , 6.61 (s, IH) , 6.60 (d, J = 8 Hz, IH) , 6.54 (s, IH) , 4.20 (m, 4H) , 2.58 (m, 4H) , 2.33 (quintet, J = 6 Hz,

2H) , 1.48 (hextet, J = 8 Hz, 2H) , 1.17 (t, J = 8 Hz, 3H) ,

+ 0.86 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated

for C₃₀H₃₃N₂O₆ (p+1) : m/z = 517.2339. Found: 517.2334.

-1 IR (CHC1₃, cm ) 2965, 1738, 1454.

Anal. Calcd for C₃₀H₃₂N₂O₆: C, 69.75; H, 6.24; N, 5.42. Found: C, 69.73; H, 6.33; N, 5.25.

Example 5 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-isoxazol-5-yl- phenoxy)propoxy] -2-propylphenoxy} enzoic acid.

A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-isoxazol- 5-yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester. A mixture of 2- (3- {3- [5-benzyloxy-4- (3- dimethylaminoacryloyl) -2-ethylphenoxy] ropoxy} -2- propylphenoxy) benzoic acid methyl ester (280 mg, 0.43 mmol), hydroxylamine hydrochloride (75 mg, 1.1 mmol), and water (1 mL) in methanol (4 mL) was heated at reflux for 2 h. The mixture was cooled to room temperature and diluted with diethyl ether and water. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 202 mg (76%) of the title compound as a

1 white solid. H NMR (CDC1₃) δ 8.20 (d, J = 2 Hz , IH) , 7.88

(dd, J = 9, 2 Hz, IH) , 7.79 (s, IH) , 7.40 (m, 7H) , 7.08 (m, 2H) , 6.68 (d, J = 8 Hz, IH) , 6.59 (s, IH) , 6.58 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.15 (s, 2H) , 4.21 (t, J = 6 Hz, 4H) , 3.82 (s, 3H) , 2.65 (m, 4H) , 2.33 (quintet, J = 6 Hz,

2H) , 1.56 (hextet, J = 8 Hz, 2H) , 1.20 (t, J = 7 Hz, 3H) ,

+ 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated

for C₃₈H₄₀NO₇ (p+1) : m/z = 622.2805. Found: 622.2817. IR

-1 (CHCI3, cm ) 2964, 1720, 1461.

Anal. Calcd for C₃₈H₃₉N0₇: C, 73.41; H, 6.32; N, 2.25. Found: C, 73.20; H, 6.34; N, 2.27.

B. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-isoxazol-5- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A solution of 2- {3- [3- (5-benzyloxy-2-ethyl-4-isoxazol-5-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (180 mg, 0.289 mmol) in ethanethiol (5 mL) was treated with boron trifluoride etherate (1.5 mL) at room temperature for 2 h, at which time an additional portion of boron trifluoride etherate (0.5 mL) was added and stirring resumed for an additional 1 h. The mixture was diluted with diethyl ether and water. The organic layer was separated, washed once with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided

94 mg (61%) of the title compound as a colorless oil H

NMR (CDC1₃) δ 8.28 (d, J = 1 Hz, IH) , 7.88 (dd, J = 8, 2 Hz, IH) , 7.38 (t, J = 8 Hz,lH), 7.36 (s, IH) , 7.08 (t, J = 8 Hz, IH) , 7.05 (d, J = 8 Hz, IH) , 6.81 (d, J = 8 Hz, IH) , 6.67 (d, J = 8 Hz, IH) , 6.50 (s, IH) , 6.45 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.62 (m, 4H) , 2.34

(quintet, J = 6 Hz, 2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.18

+ (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact

mass calculated for

(p+1) : m/z = 532.2335.

-1 Found: 532.2335. IR (CHCI3, cm ) 2964, 1715, 1601, 1461.

Anal. Calcd for C₃₁H₃₃N0₇ : C, 70.04; H, 6.26; N, 2.63. Found: C, 70.13; H, 6.35; N, 2.63.

C. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-isoxazol-5- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid.

To a solution of 2-{3- [3- (2-ethyl-5-hydroxy-4-isoxazol-5-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (94 mg, 0.18 mmol) in methanol (3 mL) was added 1 M lithium hydroxide solution (1 mL) and the resulting mixture warmed at 60 °C for 3 h. The mixture was cooled to room temperature and concentrated in vacuo . The aqueous residue was diluted with water and the pH adjusted to ~4. The mixture was extracted three times with methylene chloride. The combined organic extracts were dried (sodium sulfate), filtered, and concentrated in vacuo to provide 12 mg (13%)

1 of the title compound as an off-white amorphous solid. H

NMR (CDC1₃) δ 8.26 (s, IH) , 8.20 (dd, J = 8, 1 Hz, IH) , 7.49

(t, J = 6 Hz, IH) , 7.36 (s, IH) , 7.18 (d, J = 8 Hz, IH) , 7.15 (d, J = 8 Hz, IH) , 7.02 (bs, IH) , 6.80 (d, J = 8 Hz, IH) , 6.69 (d, J = 8 Hz, IH) , 6.60 (d, J = 8 Hz, IH) , 6.50 (s, IH) , 6.46 (s, IH) , 4.22 (t, J = 6 Hz, 2H) , 4.19 (t, J = 6 Hz, 2H) ; 2.57 (m, 4H) , 2.34 (quintet, J = 6 Hz, 2H) , 1.47

(hextet, J = 8 Hz, 2H) , 1.16 (t, J = 8 Hz, 3H) , 0.85 (t, J =

+ 7 Hz, 3H) ; TOS MS ES exact mass calculated for C₃₀H₃₂NO₇

(p+1) : m/z = 518.2179. Found: 518.2175.

Anal. Calcd for C₃₀H₃₁NO₇: C, 69.62; H, 6.04; N, 2.71. Found: C, 69.57; H, 6.15; N, 2.74.

Example 6

Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (3H- [1, 2 , 3 ] triazol-4-yl)phenoxy] propoxy} -2-propylphenoxy)benzoic acid.

A. Preparation of 2-{3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy)propoxy] -2-propylphenoxy} -benzoic acid methyl ester.

A mixture of 5-benzyloxy-4-bromo-l- (3-chloropropoxy) -2- ethylbenzene (1.19 g, 3.11 mmol), 2- (3-hydroxy-2- propylphenoxy) benzoic acid methyl ester (0.89 g, 3.1 mmol), potassium carbonate (1.29 g, 9.34 mmol), potassium iodide (0.52 g, 3.1 mmol), and methyl sulfoxide (2 mL) in 2- butanone (20 mL) was heated at reflux for 48 h. The mixture was cooled to room temperature, diluted with diethyl ether, and washed once with water. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 6% ethyl acetate/94% hexane) of the residue provided 1.34 g (68%) of the title compound as a

1 colorless oil. H NMR (CDC1₃) δ 7.91 (dd, J = 8, 2 Hz, IH) ,

7.50 (d, J = 7 Hz, 2H) , 7.38 (m, 5H) , 7.15 (d, J = 8 Hz, IH) , 7.10 (d, J = 8 Hz, IH) , 6.83 (d, J = 8 Hz, IH) , 6.71 (d, J = 8 Hz, IH) , 6.55 (s, IH) , 6.48 (, J = 8 Hz, IH) , 5.16 (s, 2H) , 4.21 (t, J = 6 Hz, 2H) , 4.15 (t, J = 6 Hz, 2H) , 3.83 (s, 3H) , 2.68 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2 . 31 ( quintet , J = 6 Hz , 2H) , 1 . 58 (hextet , J = 6 Hz , 2H) , 1 . 17 ( t , J = 7 Hz , 3H) , 0 . 93 ( t , J = 7 Hz , 3H) .

B. Preparation of 2- {3- [3- (5-benzyloxy-2-ethyl-4- ethynylphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.

A mixture of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (1.50 g, 2.37 mmol), tri-n-butylethynyltin (0.82 mL, 2.8 mmol), and tetrakis (triphenylphosphine) palladium (0) (1.0 g, 0.95 mmol) in N,N-dimethylformamide (25 mL) was purged with argon and heated in a sealed tube at 120 °C for 24 h. The mixture was cooled to room temperature and filtered. The filtrate was diluted with ethyl acetate, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 532 mg

1 (39%) of the title compound as a brown oil. H NMR (CDCI3) δ 7.88 (dd, J = 8, 2 Hz, IH) , 7.79 (s, IH) , 7.20-7.50 (m, 6H) , 7.10 (d, J = 8 Hz, IH) , 7.05 (d, J = 8 Hz, IH) , 6.80 (d, J = 8 Hz, IH) , 6.66 (d, J = 8 Hz, IH) , 6.43 (m, 2H) , 5.16 (s, 2H) , 4.17 (t, J = 6 Hz, 2H) , 4.11 (t, J = 6 Hz, 2H) , 3.83 (s, 3H) , 3.23 (s, IH) , 2.64 (t, J = 8 Hz, 2H) , 2.53 (q, J = 7 Hz, 2H) , 2.27 (quintet, J = 6 Hz, 2H) , 1.53

(m, 2H) , 1.13 (t, J = 7 Hz, 3H) , 0.89 (t, J = 7 Hz, 3H) ; TOF

+ MS ES exact mass calculated for C3₇H₃₉0₆ (p+1) : m/z =

579.2747. Found: 579.2739.

C. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (3H- [1,2,3] triazol-4-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid methyl ester. A mixture of 2- {3- [3- (5-benzyloxy-2-ethyl-4- ethynylphenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (517 mg, 0.893 mmol) and trimethylsilyl azide (3.0 mL, 18 mmol) was heated in toluene (20 mL) in a sealed tube at 130 °C for 120 h. The mixture was cooled to room temperature and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane to 50% ethyl acetate/50% hexane) of the residue provided 347 mg (88% based upon recovered starting material) of the title

1 compound as a brown solid. H NMR (CDC1₃) δ 8.10 (bs, IH) ,

7.89 (dd, J = 8, 2 Hz, IH) , 7.76 (s, IH) , 7.40 (m, 7H) , 7.10 (d, J = 8 Hz, IH) , 7.05 (d, J = 8 Hz, IH) , 6.79 (d, J = 8 Hz, IH) , 6.67 (d, J = 8 Hz, IH) , 6.62 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.18 (s, 2H) , 4.21 (m, 4H) , 3.82 (s, 3H) , 2.65 (m, 4H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.56 (hextet, J = 8

Hz, 2H) , 1.21 (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF

+ MS ES exact mass calculated for C3₇H_4QN₃0₆ (p+1) : m/z =

-1 622.2917. Found: 622.2946. IR (CHCI3, cm ) 3400, 1721,

1602, 1453.

Anal. Calcd for C₃₇H₃₉N₃0₆: C, 71.48; H, 6.32; N, 6.76.

Found: C, 70.28; H, 6.07; N, 6.54.

D. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (3H- [1,2,3] triazol-4-yl)phenoxy] -propoxy} -2-propylphenoxy)benzoic acid methyl ester. A solution of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (3H- [1,2,3] triazol-4-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (330 mg, 0.531 mmol) in ethanethiol (9 mL) was treated with boron trifluoride etherate (2.0 mL,16 mmol) for 1 h at room temperature and then with an additional portion of boron trifluoride etherate (1.0 mL) for 1 h. The mixture was diluted with diethyl ether and water. The organic layer was washed once with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane to 50% ethyl acetate/50% hexane) of the residue provided 180 mg (63%) of the title compound as a brown

1 solid. H NMR (CDC1₃) δ 7.97 (s, IH) , 7.88 (dd, J = 8, 2 Hz, IH) , 7.37 (t, J = 8 Hz, IH) , 7.31 (s, IH) , 7.10 (d, J =

8 Hz, IH) , 7.05 (d, J = 8 Hz, IH) , 6.81 (d, J = 8 Hz, IH) ,

6.67 (d, J = 8 Hz, IH) , 6.59 (s, IH) , 6.43 (d, J = 8 Hz,

IH) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.63 (m, 4H) , 2.34

(quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.19

+ (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact

mass calculated for C₃₀H₃₄N₃O₆ (p+1): m/z = 532.2447.

-1 Found: 532.2466. IR (CHCI3, cm ) 2964, 1718, 1453.

Anal. Calcd for C₃₀H33N₃O₆: C, 67.78; H, 6.26; N, 7.90.

Found: C, 66.80; H, 6.02; N, 7.53.

E. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (3H- [1,2,3] triazol-4-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid.

A solution of 2- (3- {3- [2-ethyl-5-hydroxy-4- (3H- [1,2,3] triazol-4-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (160 mg, 0.30 mmol) in methanol (5 mL) was treated 1 N lithium hydroxide solution (1.5 mL) at 60 °C for 3.5 h. The mixture was cooled to room temperature, diluted with water, and adjusted to ~pH 4. The resulting mixture was extracted three times with methylene chloride. The combined organic extracts were dried (sodium sulfate) , filtered, and concentrated in vacuo to provide 134 mg (86%)

1 of the title compound as a tan solid. H NMR (DMSO-d) δ 14.98 (bs, IH) , 12.80 (bs, IH) , 10.02 (bs, IH) , 8.17 (bs,

IH) , 7.77 (dd, J = 7, 2 Hz, IH) , 7.60 (bs, IH) , 7.47 (t, J =

8 Hz, IH) , 7.18 (t, J = 8 Hz, IH) , 7.14 (t, J = 8 Hz, IH) ,

6.82 (d, J = 8 Hz, IH) , 6.68 (d, J = 8 Hz, IH) , 6.57 (s, IH) , 6.35 (d, J = 8 Hz, IH) , 4.22 (t, J = 6 Hz, 2H) , 4.15

(t, J = 6 Hz, 2H) , 2.54 (m, 4H) , 2.25 (quintet, J = 6 Hz , 2H) , 1 . 45 (hextet , J = 8 Hz , 2H) , 1 . 11 ( t , J = 7 Hz , 3H) ,

+ 0.81 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated

for C₂₉H₃₂N₃0₆ (p+1) : m/z = 518.2291. Found: 518.2302.

-1 IR (CHC1₃, cm ) 2965, 1738, 1454.

Anal. Calcd for C₂₉H₃₁N₃0₆: C, 67.30; H, 6.04; N, 8.12. Found: C, 67.15; H, 5.98; N, 7.93.

Example 7

Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-pyrrol-1-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.

A. Preparation of 5-benzyloxy-2-ethyl-4-pyrrol-l-yl-phenol.

To a mixture of potassium nitrosodisulfonate (40.0 g, 149 mmol) and potassium hydrogen phosphate (10 g) in water (1.2 L) at room temperature was added a solution of 4- ethylbenzene-1, 3-diol (10.0 g, 2.37 mmol) and potassium hydrogen phosphate (10.5 g) in water (150 mL) . The mixture was stirred for 15 min and adjusted to pH ~3. The solution was extracted three times with diethyl ether. The organic layer was dried (sodium sulfate) , filtered, and concentrated in vacuo. The residue was dissolved in acetonitrile (70 mL) and treated at room temperature with 65% 3-pyrroline (12 mL) . The resulting mixture was stirred for 1 h and concentrated in vacuo, dissolved in ethyl acetate and hexane, and filtered down a short column of silica gel. The resulting solution was concentrated in vacuo. The residue was dissolved in N,N-dimethylformamide (10 mL) and treated with benzyl bromide (0.85 mL, 7.1 mmol) and potassium carbonate (960 mg, 6.9 mmol) at room temperature for 15 h. The mixture was diluted with ethyl acetate, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, ethyl acetate/hexane gradient) of the residue provided 316 mg (2%) of the title

+ compound. TOF MS ES exact mass calculated for C-L₉H_2QN0₂

(p+1) : m/z = 294.1494. Found: 294.1471.

B. Preparation of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] -IH-pyrrole.

A mixture of 5-benzyloxy-2-ethyl-4-pyrrol-l-yl-phenol (316 mg, 1.08 mmol), potassium carbonate (223 mg, 1.62 mmol), and l-bromo-3-chloropropane (0.16 mL, 1.6 mmol) in N,N- dimethylformamide (5 mL) was stirred at room temperature for 18 h. The mixture was diluted with ethyl acetate and water, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 314 mg (79%) of

+ the title compound as a colorless oil. TOF MS ES exact

mass calculated for C₂₂H₂₅NC10₂ (p+1): m/z = 370.1574. Found: 370.1548.

C. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-pyrrol-l- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A mixture of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] -IH-pyrrole (310 mg, 0.85 mmol) and sodium iodide (140 mg, 0.94 mol) in 2-butanone (5 mL) was heated at reflux for 6 h. The mixture was cooled to room temperature, filtered, and concentrated in vacuo. The residue was dissolved in N,N-dimethyIformamide (7 mL) and treated with 2- ( 3-hydroxy-2-propylphenoxy) benzoic acid methyl ester (242 mg, 0.85 mmol) and potassium carbonate (129 g, 93 mmol) at room temperature for 15 h. The mixture was diluted with ethyl acetate and water, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 196 mg (37%) of the title compound as a

1 colorless oil. H NMR (CDC1₃) δ 7.86 (dd, J = 8, 2 Hz, IH) ,

7.37 (dt, J = 8, 2 Hz, IH) , 7.30 (m, 5H) , 7.07 (m, 3H) , 6.84

(m, 2H) , 6.79 (d, J = 8 Hz, IH) , 6.65 (d, J = 8 Hz, IH) ,

6.58 (s, IH) , 6.42 (d, J = 8 Hz, IH) , 6.29 (m, 2H) , 4.92 (s,

2H) , 4.17 (t, J = 6 Hz, 2H) , 4.15 (t, J = 6 Hz, 2H) , 3.83

(s, 3H) , 2.65 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) ,

2.30 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) ,

+ 1.16 (t, J = 7 Hz, 3H) , 0.80 (t, J = 7 Hz, 3H) ; TOF MS ES

exact mass calculated for C₃₉H₄₂NOg (p+1) : m/z = 620.3012. Found: 620.3021.

D. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-pyrrol-l-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.

A solution of 2-{3- [3- (5-benzyloxy-2-ethyl-4-pyrrol-l-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (195 mg, 0.315 mmol) in ethanethiol (5 mL) was treated with boron trifluoride etherate (1.3 mL, 9.5 mmol) at room temperature for 2.5 h. The mixture was diluted with diethyl ether and water. The organic layer was washed with saturated sodium bicarbonate solution, dried (sodium sulfate), filtered, and concentrated in vacuo.

Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 39 mg (23%) of the title compound as a

1 colorless oil. H NMR (CDC1₃) δ 7.89 (d, J = 8 Hz, IH) ,

7.37 (t, J = 8 Hz, IH) , 7.07 (m, 2H) , 6.98 (s, IH) , 6.68 (m, 3H) , 6.65 (d, J = 8 Hz, IH) , 6.57 (s, IH) , 6.42 (d, J = 8

Hz, IH) , 6.35 (m, 2H) , 5.04 (bs, IH) , 4.19 (m, 2H) , 3.83 (s,

3H) , 2.64 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.32

(quintet, J = 6 Hz, 2H) , 1.55 (m, 2H) , 1.14 (t, J = 7 Hz,

+ 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass

calculated for C₃₂H₃₆N0₆ (p+1): m/z = 530.2543. Found: 530.2516.

Example 8

Preparation of 2- (3-{3- [4- (3-Bromo- [1,2,4] thiadiazol-5-yl) • 2-ethyl-5-hydroxyphenoxy] -propoxy} -2-propylphenoxy)benzoic acid.

A. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (4,4,5,5- tetramethyl- [1,3, 2] dioxaborolan-2-yl)phenoxy]propoxy} -2- propylphenoxy)benzoic acid methyl ester.

A mixture of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (8.30 g, 13.1 mmol), triethylamine (5.2 mL, 39 mmol), and PdCl₂(dppf) (320 mg, 0.39 mmol) in de-oxygenated toluene (80 mL) was treated with a 1 M solution of 4,4,5,5- tetramethyl- [1, 3 , 2 ] dioxaborolane in tetrahydrofuran (20 mL, 20 mmol) and heated at reflux for 6 h. The mixture was filtered down a short column of silica gel and the filtrate concentrated in vacuo. Chromatography (silica gel, 35% ethyl acetate/65% hexane) of the residue provided a dark oil that was subjected to further chromatography (silica gel, hexane to 30% ethyl acetate/70% hexane) to give 7.70 g (84%)

1 of the title compound. H NMR (CDC1₃) δ 7.86 (dd, J = 8, 2

Hz, IH) , 7.60 (d, J = 8 Hz, 2H) , 7.47 (s, IH) , 7.34 (m, 3H) , 7.24 (t, J = 8 Hz, IH) , 7.09 (d, J = 9 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.79 (d, J = 9 Hz, IH) , 6.66 (d, J = 9 Hz, IH) , 6.47 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.07 (s, 2H) , 4.18 (m, 4H) , 3.81 (s, 3H) , 2.64 (t, J = 8 Hz, 2H) , 2.56 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.53 (hextet, J = 8

Hz, 2H) , 1.34 (s, 12H),1.14 (t, J = 7 Hz, 3H) , 0.89 (t, J =

+ 7 Hz, 3H) ; TOF MS ES exact mass calculated for C₄₁H₅₃NBOg

(p + NH₄) : m/z = 698.3864. Found: 698.3889. IR (CHCl₃,

-1 cm ) 2964, 1720, 1604, 1453.

Anal. Calcd for C₄₁H₄₉BOg: C, 72.35; H, 7.26. Found: C, 72.30; H, 7.12.

B. Preparation of 2- (3-{3- [5-benzyloxy-4- (3-bromo- [1,2,4] thiadiazol-5-yl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy)benzoic acid methyl ester.

A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4, 4 , 5, 5- tetramethyl- [1,3,2] dioxaborolan-2-y1 ) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (310 mg, 0.46 mmol), 3-bromo-5-chloro-1, 2 , 4-thiadiazole (120 mg, 0.60 mmol), cesium carbonate (300 mg, 0.92 mmol), and PdCl₂(dppf) (20 mg, 0.024 mmol) in de-oxygenated toluene (10 mL) was heated at 100 °C for 15 h. The mixture was diluted with a solution of 35% ethyl acetate/65% hexane and filtered down a short column of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, hexane to 30% ethyl acetate/70% hexane) of the residue provided 232 mg (70%) of

1 the title compound. H NMR (CDCl₃) δ 8.13 (s, IH) , 7.87

(dd, J = 8, 2 Hz, IH) , 7.44 (m, 2H) , 7.37 (m, 4H) , 7.08 (t, dJ = 8, 1 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.78 (d, J = 9

Hz, IH) , 6.66 (d, J = 9 Hz, IH) , 6.55 (s, IH) , 6.43 (d, J = 8 Hz, IH) , 5.28 (s, 2H) , 4.21 (t, J = 6 Hz, 2H) , 4.19 (t, J = 6 Hz, 2H) , 3.81 (s, 3H) , 2.62 (m, 4H) , 2.34 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.17 (t, J = 7 Hz, 3H) , 0.88 (t, J = 7 Hz, 3H) ; MS ES m/e 717, 719.

C. Preparation of 2- (3-{3- [4- (3-bromo- [1,2,4] thiadiazol-5- y1) -2-ethyl-5-hydroxyphenoxy]propoxy} -2- propylphenoxy)benzoic acid.

A solution of 2- (3-{3- [5-benzyloxy-4- (3-bromo- [1,2,4] thiadiazol-5-yl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (230 mg, 0.31 mmol) in ethanethiol (4 mL) was treated with boron trifluoride etherate (0.32 mL, 2.5 mmol) at room temperature for 6 h, at which time an additional portion of boron trifluoride etherate was added and stirring continued for 7 h. The reaction mixture was diluted with water, concentrated in vacuo, and extracted with diethyl ether. The residue was dissolved in methanol (5 mL) and treated with 1 N lithium hydroxide solution (2 mL) at 65 °C for 1 h. The mixture was concentrated in vacuo and the residue diluted with water and adjusted to ~pH 3 with 1 N hydrochloric acid. The resulting precipitate was collected via vacuum filtration and dissolved in dilute aqueous base. Reverse phase chromatography (1:1 acetonitrile/water) provided 43 mg (23%)

1 of the title compound as a yellow solid. H NMR (DMSO-dg) δ

7.85 (s, IH) , 7.80 (dd, J = 8, 2 Hz, IH) , 7.45 (m, 2H) , 7.15 (m, 3H) , 6.83 (d, J = 9 Hz, IH) , 6.80 (d, J = 9 Hz, IH) , 6.62 (s, IH) , 6.35 (d, J = 9 Hz, IH) , 4.20 (m, 4H) , 2.55 (m,

4H) , 2.27 (quintet, J = 5 Hz, 2H) , 1.44 (hextet, J = 8 Hz,

+ 2H) , 1.13 (t, J = 7 Hz, 3H) , 0.81 (t, J = 7 Hz, 3H) ; MS ES

+ -1 m/e 551 (p+NH₄ -Br) ; IR (KBr, cm ) 2900, 1696, 1603, 1461.

Anal. Calcd for C₂₉H₂₉BrN₂0gS : C, 56.77; H, 4.76; N, 4.56. Found: C, 56.63; H, 4.72; N, 3.98. Example 9

Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-thiophen-2-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid sodium salt.

A. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiophen-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4 , 4 , 5 , 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (300 mg, 0.44 mmol), 2-bromothiophene (110 mg, 0.66 mmol), cesium carbonate (300 mg, 2.17 mmol), and PdCl₂(dppf) (20 mg, 0.024 mmol) in de- oxygenated toluene (10 mL) was heated at 105 °C for 66 h. The mixture was cooled to room temperature and concentrated in vacuo. The residue was dissolved in methylene chloride and filtered down a short column of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane) of the residue provided an oil that was dissolved in ethanethiol (4 mL) and treated with boron trifluoride etherate (0.44 mL, 3.4 mmol) at room temperature for 3 h. The mixture was diluted with water and extracted with diethyl ether. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, hexane to 30% ethyl acetate/70% hexane) of the residue provided 120 mg (50%) of the title

1 compound as a yellow film. H NMR (CDCl₃) δ 7.85 (dd, J =

8, 2 Hz, IH) , 7.35 (t, J = 8 Hz, IH) , 7.15 (d, J = 7 Hz, 1H) , 7.03-7.15 (m, 5H) , 6.80 (d, J = 9 Hz, IH) , 6.66 (d, J 9 Hz, IH) , 6.51 (s, IH) , 6.42 (d, J = 8 Hz, IH) , 5.44 (bs, IH) , 4.18 (m, 4H) , 3.82 (s, 3H) , 2.62 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.54 (quintet, J = 6 Hz, 2H) , 1.52 (hextet, J = 8 Hz, 2H) , 1.16 (t, J = 7 Hz, 3H) , 0.90 (t, J

7 Hz, 3H) ; MS ES m/e 545 (p - 1) .

B. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiophen-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid sodium salt.

A solution of 2- {3- [3- (2-ethyl-5-hydroxy-4-thiophen-2-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (120 mg, 0.22 mmol) in methanol (3 mL) was treated with 1 N lithium hydroxide solution (0.5 mL) at room temperature for 1 h and then with an additional portion of 1 N lithium hydroxide solution (0.75 mL) for 18 h. The mixture was heated at 50 °C then concentrated in vacuo. The residue was acidified with dilute hydrochloric acid and extracted with diethyl ether. The organic layer was washed once with water and concentrated in vacuo. The residue was diluted with 1 N sodium hydroxide solution (0.22 mL) , diethyl ether, and toluene. The mixture was concentrated in vacuo, dissolved in methylene chloride, and concentrated in vacuo to provide

1 120 mg (98%) of the title compound as a green film. H NMR

(DMSO-dg) δ 7.71 (d, J = 8 Hz, IH) , 7.42 (m, 2H) , 7.31 (m,

2H) , 7.10 ( , 2H) , 6.99 (m, IH) , 6.76 (t, J = 7 Hz, 2H) , 6.52 (s, IH) , 6.30 (d, J = 8 Hz, IH) , 4.16 (t, J = 7 Hz, 2H) , 4.07 (t, J = 7 Hz, 2H) , 2.50 (m, 4H) , 2.20 (m, 2H) , 1.40 (m, 2H) , 1.06 (t, J = 8 Hz, 3H) , 0.77 (t, J = 7 Hz,

+ + -1

3H) ; MS ES m/e 533 (p + 1 - Na ) . IR (CHCl₃, cm ) 2900,

1738, 1604, 1454.

Example 10 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (1-methyl-lH- pyrazol-4-yl) -phenoxy]propoxy} -2-propylphenoxy)benzoic acid.

A. Preparation of 4-iodo-l-methylpyrazole (Known compound: RN 39806-90-1) .

To a solution of 4-iodopyrazole (1.3 g, 6.8 mmol) in dioxane (10 mL) was added iodomethane (0.42 mL, 6.8 mmol) and the resulting mixture stirred at room temperature for 96 h. The mixture was concentrated in vacuo and the residue mixed with methylene chloride and filtered. The filtrate was concentrated in vacuo to provide 1.35 g (95%) of the title 1 compound as a colorless oil. H NMR (CDCI3) δ 7.47 (s, IH) ,

7.38 (s, IH) , 3.90 (s, 3H) .

B. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (1-methyl- lH-pyrazol-4-yl)phenoxy] -propoxy} -2-propylphenoxy)benzoic acid methyl ester.

A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4, 4, 5, 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (1.00 g, 1.47 mmol), 4-iodo-l-methylpyrazole (450 mg, 2.16 mmol), cesium carbonate (1.20 g, 3.62 mmol), and PdCl₂(dppf) (72 mg, 0.088 mmol) in de-oxygenated toluene (35 mL) was heated at 100 °C for 24 h. Additional portions of 4-iodo-l-methylpyrazole

(-30 mg) and PdCl₂(dppf) (-30 mg) were added and heating continued at 100 °C for 40 h. The mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, 35% ethyl acetate/65% hexane to 65% ethyl acetate/35% hexane) of the residue provided 710 mg

1 (76%) of the title compound. H NMR (CDCI3) δ 7.86 (dd, J =

8, 2 Hz, IH) , 7.80 (s, IH) , 7.69 (s, IH) , 7.37 (m, 6H) , 7.28 (s, IH) , 7.09 (d, J = 9 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.78 (d, J = 9 Hz, IH) , 6.67 (d, J = 9 Hz, IH) , 6.56 (s, IH) , 6.42 (d, J = 8 Hz, IH) , 5.08 (s, 2H) , 4.18 (t, J = 6

Hz, 2H) , 4.15 (t, J = 6 Hz, 2H) , 3.85 (s, 3H) , 3.81 (s, 3H) , 2.63 (t, J = 8 Hz, 2H) , 2.59 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.23 (t, J = 7 Hz, 3H) , 0.89 (t, J = 7 Hz, 3H) .

C. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (l-methyl- lJϊ-pyrazol^-yl) -phenoxy]propoxy} -2-propylphenoxy)benzoic acid.

A solution of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (1-methyl-lH- pyrazol-4-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (710 mg, 1.12 mmol) in ethanethiol (5 mL) was treated with boron trifluoride etherate (1.42 mL, 11.2 mmol) at room temperature for 20 h. The reaction mixture was diluted with water, concentrated in vacuo, and extracted with diethyl ether. The organic layer was dried (magnesium sulfate), filtered, and concentrated in vacuo. The residue was triturated twice with hexane and the residue dissolved in methanol (5 mL) . This solution was treated with 1 N lithium hydroxide solution (5 mL) at -95 °C for 2 h. The mixture was concentrated in vacuo and the residue diluted with, water, washed twice with diethyl ether, and the aqueous layer acidified with 1 N hydrochloric acid. The resulting solution was extracted with diethyl ether. The organic layer was dried (magnesium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% methanol/90% methylene chloride) provided 338 mg (57%) of

1 the title compound as a tan foam. H NMR (DMSO-dg) δ 12.85

(bs, IH) , 9.50 (bs, IH) , 7.98 (s, IH) , 7.78 (m, 2H) , 7.48 (dt, J = 8, 2 Hz, IH) , 7.44 (s, IH) , 7.18 (t, J = 8 Hz, IH) , 7.13 (t, J = 9 Hz, IH) , 6.79 (d, J = 9 Hz, IH) , 6.77 (d, J = 9 Hz, IH) , 6.53 (s, IH) , 6.35 (d, J = 9 Hz, IH) , 4.20 (t, J = 6 Hz, 2H) , 4.08 (t, J = 6 Hz, 2H) , 3.85 (s, 3H) , 2.50 (m,

4H) , 2.24 (quintet, J = 5 Hz, 2H) , 1.45 (hextet, J = 8 Hz,

+ 2H) , 1.09 (t, J = 7 Hz, 3H) , 0.82 (t, J = 7 Hz, 3H) ; MS ES

-1 m/e 531 (p+1) ; IR (KBr, cm ) 2961, 1697, 1602, 1460, 1222.

Anal. Calcd for C₃₁H₃₄N₂0g: C, 70.17; H, 6.46; N, 5.28. Found: C, 69.27; H, 6.08; N, 4.63.

Example 11 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-thiazol-2-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid.

A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-thiazol-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4, 4 , 5 , 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (960 mg, 1.41 mmol), 2-bromothiazole (0.25 mL, 2.8 mmol), cesium carbonate (1.15 g, 3.52 mmol), and PdCl₂(dppf) (35 mg, 0.040 mmol) in de- oxygenated toluene (35 mL) was heated at 60 °C for 16 h then at 100 °C for 7 h. Additional portions of 2-bromothiazole

(0.13 mL) and PdCl₂(dppf) (-30 mg) were added and heating continued at 100 °C for 72 h. The mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, hexane to 35% ethyl acetate/65% hexane) of the residue provided 282 mg (31%) of the title

1 compound. H NMR (CDCI3) δ 8.20 (s, IH) , 7.86 (dd, J = 8, 1

Hz, IH) , 7.82 (d, J = 3 Hz, IH) , 7.49 (d, J = 7 Hz, 2H) , 7.35 (m, 4H) , 7.23 (d, J = 3 Hz, IH) , 7.09 (d, J = 9 Hz,

IH) , 7.04 (d, J = 9 Hz, IH) , 6.78 (d, J = 9 Hz, IH) , 6.65

(d, J = 9 Hz, IH) , 6.57 (s, IH) , 6.42 (d, J = 8 Hz, IH) ,

5.24 (s, 2H) , 4.17 (m, 4H) , 3.81 (s, 3H) , 2.63 (m, 4H) , 2.33

(quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.19

(t, J = 7 Hz, 3H) , 0.88 (t, J = 7 Hz, 3H) .

B. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A solution of 2- {3- [3- (5-benzyloxy-2-ethyl-4-thiazol-2-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (282 mg, 0.442 mmol) in ethanethiol (3 mL) was treated with boron trifluoride etherate (0.56 mL, 4.4 mmol) at room temperature for 3 h. The reaction mixture was diluted with water, concentrated in vacuo, and extracted with diethyl ether. The organic layer was dried (magnesium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, ethyl acetate/hexane) provided 107 mg (44%) of the title compound. H NMR (CDC1₃) δ 7.88 (dd, J = 8, 2 Hz,

IH) , 7.80 (d, J = 4 Hz, IH) , 7.35 (dt, J = 8, 2 Hz, IH) ,

7.28 (d, J = 4 Hz, IH) , 7.24 (s, IH) , 7.09 (dt, J = 9, 2 Hz,

IH) , 7.05 (t, J = 9 Hz, IH) , 6.79 (d, J = 9 Hz, IH) , 6.66

(d, J = 9 Hz, IH) , 6.61 (s, IH) , 6.42 (d, J = 9 Hz, IH) ,

4.24 (t, J = 6 Hz, 2H) , 4.18 (t, J = 6 Hz, 2H) , 3.81 (s,

3H) , 2.63 (t, J = 7 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.34

(quintet, J = 6 Hz, 2H) , 1.52 (hextet, J = 8 Hz, 2H) , 1.17

+ (t, J = 7 Hz, 3H) , 0.88 (t, J = 7 Hz, 3H) ; MS ES m/e 548

⁽P+1⁾ •

C. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid.

2- {3- [3- (2-Ethyl-5-hydroxy-4-thiazol-2-yl-phenoxy) propoxy] - 2-propylphenoxy}benzoic acid methyl ester (107 mg, 0.196 mmol) was dissolved in a 1:1 solution of methanol/dioxane (3 mL) and treated with 1 N lithium hydroxide solution (1 mL) at 60 °C for 2 h. The mixture was concentrated in vacuo and the residue diluted with water, washed twice with diethyl ether, and the aqueous layer acidified with 1 N hydrochloric acid. The resulting solution was extracted twice with methylene chloride and the combined organic layers dried (magnesium sulfate), filtered, and concentrated in vacuo. Trituration (hexane) of the residue provided 72 mg (69%) of the title compound as a tan powder. H NMR (CDCI3) δ 8.22

(dd, J = 8, 2 Hz, IH) , 7.70 (d, J = 4 Hz, IH) , 7.41 (dt, J =

8, 2 Hz, IH) , 7.35 (s, IH) , 7.18 (m, 3H) , 6.82 (d, J = 9 Hz,

IH) , 6.69 (d, J = 9 Hz, IH) , 6.62 (d, J = 9 Hz, IH) , 6.55 (s, IH) , 4.22 (t, J = 6 Hz, 2H) , 4.21 (t, J = 6 Hz, 2H) ,

2.57 (m, 4H) , 2.35 (quintet, J = 6 Hz, 2H) , 1.49 (hextet, J

= 8 Hz, 2H) , 1.18 (t, J = 7 Hz, 3H) , 0.86 (t, J = 7 Hz, 3H) ;

+ -1

MS ES m/e 534 (p+1) ; IR (KBr, cm ) 2957, 1695, 1599, 1457.

Anal. Calcd for C₃₀H₃₁NO₆S: C, 67.52; H, 5.86; N, 2.62. Found: C, 67.44; H, 5.95; N, 2.55.

Example 12 Preparation of 2- (3-{3- [4- (3, 5-Dimethylisoxazol-4-yl) -2- ethyl-5-hydroxyphenoxy]propoxy} -2-propylphenoxy)benzoic acid sodium salt.

A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4 , 4 , 5 , 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (305 mg, 0.448 mmol), 3 , 5-dimethyl-4-iodoisoxazole (110 mg, 0.493 mmol), cesium carbonate (293 mg, 0.899 mmol), and PdCl₂(dppf) (15 mg, 0.018 mmol) in de-oxygenated toluene (10 mL) was heated at 95 °C for 10 h. Additional portions of 3 , 5-dimethyl-4- iodoisoxazole (110 mg) , cesium carbonate (260 mg) , and

PdCl₂(dppf) (-15 mg) were added and heating continued at 110

°C for 20 h. The mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel with 20% ethyl acetate/80% hexane. The filtrate was concentrated in vacuo. The resulting colorless oil was dissolved in methylene chloride (4 mL) , cooled to 0 °C, and treated with iodotrimethylsilane (0.40 mL, 2.7 mmol). The resulting mixture was allowed to warm to room temperature and stirred for 18 h. An additional portion of iodotrimethylsilane (0.70 mL) was added and stirring continued for 72 h. The mixture was poured into dilute sodium thiosulfate solution. The organic layer was separated, washed with water, dried (sodium sulfate), filtered, and concentrated in vacuo. The resulting foam was dissolved in a 1:1 mixture of tetrahydrofuran/1 N hydrochloric acid (5 mL) and stirred at room temperature for 18 h. The mixture was concentrated in vacuo and treated with 1 equivalent 1 N sodium hydroxide solution in ether. The resulting mixture was concentrated in vacuo to provide 59 mg (23%) of the title compound as an

1 off-white solid. H NMR (DMSO-dg) δ 7.40 (dd, J = 9, 2 Hz ,

IH) , 7.13 (dt, J = 8, 2 Hz, IH) , 6.97 (m, 2H) , 6.79 (s, IH) , 6.68 (d, J = 9 Hz, IH) , 6.65 (d, J = 9 Hz, IH) , 6.60 (s, IH) , 6.21 (d, J = 8 Hz, IH) , 4.19 (t, J = 6 Hz, 2H) , 4.01 (t, J = 6 Hz, 2H) , 2.66 (t, J = 8 Hz, 2H) , 2.48 (q, J = 8 Hz, 2H) , 2.24 (s, 3H) , 2.17 (quintet, J = 6 Hz, 2H) , 2.07

(s, 3 H) , 1.49 (hextet, J = 8 Hz, 2H) , 1.07 (t, J = 7 Hz,

+ 3H) , 0.85 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass

calculated for C₃₂H₃gN0₇ (p+1) : m/z = 546.2492. Found:

-1 546.2514; IR (KBr, cm ) 3400, 1605, 1460.

Example 13 Preparation of 2-{3- [3- (2-Ethyl-4-furan-2-yl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy} -benzoic acid sodium salt.

A. Preparation of 2-{3- [3- (4-bromo-2-ethyl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A solution of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (2.50 g, 3.95 mmol) in methylene chloride (40 mL) was cooled to -70 °C and treated with boron tribromide (0.25 mL, 2.6 mmol) . After 25 min the mixture was poured into cold water and the resulting mixture extracted with methylene chloride. The combined organic extracts were washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo to provide 1.1 g (52%) of the title compound as a pale

1 yellow oil. H NMR (CDCl₃) δ 7.89 (d, J = 9 Hz , IH) , 7.38

(t, J = 8 Hz, IH) , 7.18 (s IH) , 7.12 (d, J = 9 Hz, IH) , 7.08 (d, J = 2 Hz, IH) , 6.81 (d, J = 9 Hz, IH) , 6.68 (d, J = 9 Hz, IH) , 6.56 (s, IH) , 6.46 (d, J = 9 Hz, IH) , 5.40 (s, IH) , 4.18 (t, J = 6 Hz, 2H) , 4.11 (t, J = 6 Hz, 2H) , 3.84 (s, 3H) , 2.65 (t, J = 8 Hz, 2H) , 2.54 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.13 (t, J = 7 Hz, 3H) , 0.89 (t, J = 7 Hz, 3H) ; MS ES m/z = 541 (M - H) , 543 (M - H + 2) .

B. Preparation of 2- (3-{3- [4-bromo-5- ( tert- butyldimethylsilanyloxy) -2-ethylphenoxy] -propoxy} -2- propylphenoxy)benzoic acid methyl ester.

A solution of 2- {3- [3- (4-bromo-2-ethyl-5- hydroxyphenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (1.00 g, 1.84 mmol) in methylene chloride (20 mL) was treated with imidazole (0.19 g, 2.8 mmol) and tert- butyldimethylsilyl chloride (0.388 g, 2.57 mmol) at room temperature for 2 h. The mixture was poured into water and the organic layer separated, washed once with water, once with saturated sodium chloride solution, filtered through a short pad of silica gel, and concentrated in vacuo to provide 1.1 g (91%) of the title compound as a colorless

1 oil. H NMR (CDC1₃) δ 7.88 (d, J = 9 Hz, IH) , 7.38 (t, J = 8 Hz, IH) , 7.22 (s IH) , 7.12 (d, J = 9 Hz, IH) , 7.08 (d, J = 2 Hz, IH) , 6.80 (d, J = 9 Hz, IH) , 6.69 (d, J = 9 Hz, IH) , 6.45 (d, J = 9 Hz, IH) , 6.40 (s, IH) , 4.20 (t, J = 6 Hz, 2H) , 4.11 (t, J = 6 Hz, 2H) , 3.83 (s, 3H) , 2.64 (t, J = 8 Hz, 2H) , 2.54 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.13 (t, J = 7 Hz, 3H) , 1.03 (s, 9H) , 0.89 (t, J = 7 Hz, 3H) , 0.23 (s, 6H) .

C. Preparation of 2-{3- [3- (2-ethyl-4-furan-2-yl-5- hydroxyphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.

A mixture of 2- (3- {3- [4-bromo-5- ( ert- butyldimethylsilanyloxy) -2-ethylphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (1.05 g, 1.60 mmol), furan-2-boronic acid (0.358 g, 3.20 mmol), tetrakis ( triphenylphosphine)palladium(O) (0.185 g, 0.160 mmol) , and 2 M aqueous sodium carbonate solution (8 mL) in tetrahydrofuran (20 mL) was heated at reflux for 18 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 0.8 g

(94%) of the title compound as a colorless oil, H NMR

(CDC1₃) δ 7.90 (d, J = 9 Hz, IH) , 7.48 (s, IH) , 7.38 (t, J =

8 Hz, IH) , 7.21 (s IH) , 7.13 (s, IH) , 7.10 (d, J = 9 Hz, IH) , 7.07 (d, J = 2 Hz, IH) , 6.81 (d, J = 9 Hz, IH) , 6.69 (d, J = 9 Hz, IH) , 6.52 (m, 3H) , 6.44 (d, J = 9 Hz, IH) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.67 (t, J = 8 Hz, 2H) , 2.59 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.18 (t, J = 7 Hz, 3H) , 0.91 (t, J = 7 Hz,

3H) ; MS ES m/z = 589 (p + AcO ) .

Anal. Calcd for C₃₂H₃₄0₇ : C, 72.43; H, 6.46. Found: C, 72.21; H, 6.15.

D. Preparation of 2-{3- [3- (2-ethyl-4-furan-2-yl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy} enzoic acid sodium salt.

2- {3- [3- ( 2-Ethyl-4-furan-2-yl-5-hydroxyphenoxy) propoxy] -2- propylphenoxy}benzoic acid methyl ester (250 mg, 0.47 mmol) was dissolved in tetrahydrofuran (4 mL) and treated with 1 N lithium hydroxide solution (2 mL) at 50 °C for 16 h. The mixture was concentrated in vacuo and the residue diluted with water and extracted twice with ethyl acetate. The combined organic extracts were washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in ethyl acetate and shaken with 1 N hydrochloric acid. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in diethyl ether and treated with 1 N aqueous sodium hydroxide solution (0.32 mL) . The mixture was concentrated in vacuo and azeotroped successively with diethyl ether, chloroform, and diethyl ether and dried to provide 168 mg (66%) of the title product as a cream solid.

1 H NMR (DMSO-d₆) δ 7.56 (s, IH) , 7.44 (d, J = 8 Hz, IH) ,

7.35 (s, IH) , 7.13 (m, IH) , 6.97 (m, 2H) , 6.77 (d, J = 2 Hz, IH) , 6.65 (m, 4H) , 6.48 (d, J = 2 Hz, IH) , 6.24 (d, J = 9 Hz, IH) , 4.15 (t, J = 6 Hz, 2H) , 3.96 (t, J = 6 Hz, 2H) , 2.66 (t, J = 8 Hz, 2H) , 2.42 (q, J = 7 Hz, 2H) , 2.13

(quintet, J = 6 Hz, 2H) , 1.48 (hextet, J = 8 Hz, 2H) , 1.09

(t, J = 7 Hz, 3H) , 0.84 (t, J = 7 Hz, 3H) ; TOF MS ES

exact mass calculated for

(p+1): m/z = 517.2226.

-1 Found: 517.2230. IR (KBr, cm ) 3400, 2961, 1599, 1460.

Example 14 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4-furan-3- yl]phenoxy]propoxy} -2-propylphenoxy)benzoic acid.

A. Preparation of 2-{3- [3- (2-ethyl-4-furan-3-yl-5- hydroxyphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.

A mixture of 2- (3- {3- [4-bromo-5- ( terfc- butyldimethylsilanyloxy) -2-ethylphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (2.10 g, 3.19 mmol), furan-3-boronic acid (0.722 g, 6.45 mmol), tetrakis ( triphenylphosphine)palladium(O) (0.37 g, 0.32 mmol), and 2 M aqueous sodium carbonate solution (16 mL) in tetrahydrofuran (30 mL) was heated at reflux for 48 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided 0.29 g

+ (17%) of the title compound as a yellow oil. TOF MS ES

exact mass calculated for C₃₂H3₅0₇ (p+1) : m/z = 531.2383 Found: 531.2396.

B. Preparation of 2-{3- [3- (2-ethyl-4-furan-3-yl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy}benzoic acid sodium salt.

2-{3- [3- (2-Ethyl-4-furan-3-yl-5-hydroxyphenoxy)propoxy] -2- propylphenoxy}benzoic acid methyl ester (170 mg, 0.32 mmol) was dissolved in tetrahydrofuran (4 mL) and methanol (1 mL) and treated with 1 N lithium hydroxide solution (4 mL) at 50 °C for 2 h. The mixture was concentrated in vacuo and the residue acidified with hydrochloric acid and the resulting mixture extracted twice with ethyl acetate. The combined organic extracts were washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 2% methanol/98% chloroform) of the residue gave 45 mg of material that was again submitted to chromatography (silica gel, 1% methanol/99% chloroform) to provide 25 mg

(15%) of the title compound as an oil.

+ TOF MS ES exact mass calculated for C₃₁H3₃0₇ (p+1) : m/z =

517.226. Found: 517.2230. Example 15 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (tetrahydrofuran-3-yl)phenoxy]propoxy} -2- propylphenoxy)benzoic acid sodium salt hemihydrate.

A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-furan-3- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.

A mixture of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (3.00 g, 4.73 mmol), furan-3-boronic acid (1.06 g, 9.47 mmol), tetrakis ( triphenylphosphine)palladium(O) (0.54 g, 0.47 mmol), and 2 M aqueous sodium carbonate solution (20 mL) in tetrahydrofuran (40 mL) was heated at 100 °C for 48 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 1.9 g

1 (65%) of the title compound as a yellow oil. H NMR (CDCI3) δ 7.88 (dd, J = 8, 2 Hz, IH) , 7.87 (s, IH) , 7.40 (m, 7H) , 7.26 (s IH) , 7.05 (m, 2H) , 6.80 (d, J = 9 Hz, IH) , 6.76 (d, J = 2 Hz, IH) , 6.67 (d, J = 9 Hz, IH) , 6.60 (s, IH) , 6.43 (d, J = 9 Hz, IH) , 5.11 (s, 2H) , 4.18 (m, 4H) , 3.83 (s, 3H) , 2.66 (t, J = 8 Hz, 2H) , 2.62 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.57 (hextet, J = 8 Hz, 2H) , 1.20

(t, J = 7 Hz, 3H) , 0.92 (t, J = 7 Hz, 3H) ; MS ES m/z = 621

-1 (p + 1); IR (CHCI3, cm ) 3000, 1727, 1603, 1461.

B. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (tetrahydrofuran-3-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid methyl ester.

A solution of 2-{3- [3- (5-benzyloxy-2-ethyl-4-furan-3-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (1.8 g, 2.9 mmol) in ethyl acetate (40 mL) was treated with 10% palladium-on-carbon (0.39 g) and hydrogenated at 48 psi and 45 °C for 72 h. The mixture was cooled to room TM temperature, filtered through Celite , and the filtrate concentrated in vacuo to provide 1.2 g (77%) of the title

1 compound as a colorless oil. H NMR (CDC1 ) δ 7.88 (dd, J =

8, 2 Hz, IH) , 7.57 (dt, J = 8, 2 Hz, IH) , 7.09 (d, J = 9 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.81 (d, J = 9 Hz, IH) , 6.80 (s, IH) , 6.67 (d, J = 9 Hz, IH) , 6.44 (d, J = 9 Hz, IH) , 6.43 (s, IH) , 4.19 (m, 3H) , 4.10 (m, 2H) , 4.02 (dd, J = 12, 3 Hz, IH) , 3.88 (dd, J = 12, 8 Hz, IH) , 3.84 (s, 3H) , 3.73 (q, J = 9 Hz, IH) , 3.45 (m, IH) , 2.64 (t, J = 8 Hz, 2H) , 2.53 (q, J = 7 Hz, 2H) , 2.38 (m, IH) , 2.28 (quintet, J = 6

Hz, 2H) , 1.99 (m, IH) , 1.55 (hextet, J = 8 Hz, 2H) , 1.15 (t,

J = 7 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; MS ES m/z = 593 (p +

-1 CH3COO ); IR (CHCI3, cm ) 2963, 1719, 1589, 1461.

Anal. Calcd for C₃₂H₃₈0₇: C, 71.89; H, 7.16. Found: C, 71.41; H, 7.06.

C. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (tetrahydrofuran-3-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid sodium salt hemihydrate.

A solution of 2- (3-{3- [2-ethyl-5-hydroxy-4- ( tetrahydrofuran- 3-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (0.92 g, 1.7 mmol) in tetrahydrofuran (10 mL) and methanol (5 mL) was treated with 1 M aqueous lithium hydroxide solution (10 mL) at 55 °C for 2 h. The mixture was allowed to cool to room temperature and stirred for an additional 18 h. The mixture was concentrated in vacuo and the remaining aqueous mixture was washed once with diethyl ether. The aqueous layer was acidified with concentrated hydrochloric acid and the resulting solution extracted with ethyl acetate. The ethyl acetate layer was washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The resulting colorless oil was dissolved in diethyl ether and treated with 1 N aqueous sodium hydroxide solution (1.72 mL) . The resulting biphasic mixture was diluted with chloroform and concentrated in vacuo. Diethyl ether was added and the mixture concentrated in vacuo. The resulting white foam was dried in vacuo at room temperature for 60 h to provide 0.78 g (84%) of the title compound: mp 67-71 °C.

1 H NMR (DMSO-d₆) δ 7.62 (dd, J = 8, 2 Hz, IH) , 7.30 (dt, J = 8, 2 Hz, IH) , 7.05 (m, 2H) , 6.85 (s, IH) , 6.73 (d, J = 9 Hz,

IH) , 6.70 (d, J = 9 Hz, IH) , 6.53 (s, IH) , 6.34 (d, J = 9

Hz, IH) , 4.15 (t, J = 6 Hz, 2H) , 4.04 (t, J = 6 Hz, 2H) ,

3.95 (m, IH) , 3.88 (m, IH) , 3.75 (q, J = 9 Hz, IH) , 3.49 (m

2H) , 2.60 (t, J = 8 Hz, 2H) , 2.45 (q, J = 7 Hz, 2H) , 2.15 (m, 3H) , 1.90 (m, IH) , 1.48 (hextet, J = 8 Hz, 2H) , 1.06 (t, J = 7 Hz, 3H) , 0.83 (t, J = 7 Hz, 3H) ; MS ES m/z = 519 (p -

+ -1

Na ); IR (CHC1₃, cm ) 2964, 1783, 1604, 1461.

Anal. Calcd for C₃₁H₃₅ a0₇ . 0.5 H₂0: C, 67.50; H, 6.58. Found: C, 67.76; H, 6.68.

Example 16

Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-pyrrolidin-2-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid hydrochloride hydrate.

A. Preparation of 2- (2-benzyloxy-5-ethyl-4-{3- [3- (2- methoxycarbonylphenoxy) -2- propylphenoxy] propoxy}phenyl)pyrrole-l-carboxylic acid tert- butyl ester. A mixture of 2-{3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (3.00 g, 4.73 mmol), N-boc pyrrole-2-boronic acid (1.99 g, 9.43 mmol) , tetrakis ( triphenylphosphine)palladium(O) (0.54 g, 0.47 mmol) , and 2 M aqueous sodium carbonate solution (25 mL) in tetrahydrofuran (60 mL) was heated at reflux for 40 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 2.6 g

1 (76%) of the title compound as a solid. H NMR (CDCI3) δ

7.88 (dd, J = 8, 2 Hz, IH) , 7.15-7.40 (m, 7H) , 7.08 (m, 3H) , 6.82 (d, J = 9 Hz, IH) , 6.68 (d, J = 9 Hz, IH) , 6.52 (s, IH) , 6.44 (d, J = 9 Hz, IH) , 6.23 (t, J = 4 Hz, IH) , 6.12 (m, IH) , 4.95 (s, 2H) , 4.20 (t, J = 6 Hz, 2H) ; 4.15 (t, J = 6 Hz, 2H) , 3.84 (s, 3H) , 2.66 (t, J = 8 Hz, 2H) , 2.60 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.57 (hextet, J = 8 Hz, 2H) , 1.28 (s, 9H) , 1.18 (t, J = 7 Hz, 3H) , 0.93 (t, J

+ = 7 Hz, 3H) ; TOS MS ES exact mass calculated for

+ C₄₄H₅₃N₂0₈ (p + NH₄ ): m/z = 737.3802. Found: 737.3804;

-1 IR (CHCI3, cm ) 2964, 1730, 1461.

Anal. Calcd for C₄₄H₄₉N0₈: C, 73.41; H, 6.86; N, 1.94. Found: C, 73.76; H, 6.76; N, 2.04.

B. Preparation of 2- (5-ethyl-2-hydroxy-4-{3- [3- (2- methoxycarbonylphenoxy) -2-propylphenoxy]propoxy}phenyl) - pyrrolidine-1-carboxylic acid tert-butyl ester.

A solution of 2-(2-benzyloxy-5-ethyl-4-{3-[3-(2- methoxycarbonylphenoxy) -2- propylphenoxy]propoxy}phenyl)pyrrole-l-carboxylic acid tert- butyl ester (0.98 g, 1.4 mmol) in ethyl acetate (40 mL) was treated with 10% palladium-on-carbon (0.98 g) and hydrogenated at 45 psi and 45 °C for 25 h, at room temperature for 20 h, then at 45 °C for 19 h. The mixture

TM was cooled to room temperature, filtered through Celite , and the filtrate concentrated in vacuo to provide 0.76 g

(88%) of the title compound as a colorless oil H NMR

(CDC1₃) δ 7.87 (dd, J = 8, 2 Hz, IH) , 7.37 (dt, J = 8, 2 Hz,

IH) , 7.10 (d, J = 9 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.91 (s, IH) , 6.81 (d, J = 9 Hz, IH) , 6.67 (d, J = 9 Hz, IH) , 6.47 (s, IH) , 6.44 (d, J = 9 Hz, IH) , 5.09 (m, IH) , 4.18 (d, J = 6 Hz, 2H) , 4.14 (t, J = 6 Hz, 2H) , 3.84 (s, 3H) , 3.45 (m, 2H) , 2.64 (t, J = 8 Hz, 2H) , 2.54 (m, 3H) , 2.25 (m, 5H) , 2.06 (m, IH) , 1.54 (hextet, J = 8 Hz, 2H) , 1.43 (s, 9H) , 1.15 (t, J = 7 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) .

C. Preparation of 2- (4-{3- [3- (2-carboxyphenoxy) -2- propylphenoxy]propoxy} -5-ethyl-2-hydroxyphenyl)pyrrolidine-

1-carboxylic acid tert-butyl ester lithium salt hydrate. A solution of 2- (5-ethyl-2-hydroxy-4- {3- [3- (2- methoxycarbonylphenoxy) -2- propylphenoxy] propoxy}phenyl ) pyrrolidine-1-carboxylic acid tert-butyl ester (0.114 g, 0.18 mmol) in a 1:1 mixture of methanol/tetrahydrofuran (4 mL) was treated with solution of 1 M lithium hydroxide (4 mL) at room temperature for 18 h. The mixture was concentrated in vacuo and the residue dissolved in water. The resulting mixure was extracted with ethyl acetate. The organic extract was dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was diluted with diethyl ether, concentrated in vacuo, and

+ dried to provide 90 mg (78%) of the title compound. MS ES + -1 m/z = 620 (p + 1 - Li ) ; IR (KBr, cm ) 2964, 1672, 1603,

1416.

Anal. Calcd for C₃gH₄₄ 0₈Li . H₂0: C, 67.17; H, 7.20; N,

2.18. Found: C, 66.72; H, 6.99; N, 2.27.

D. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-pyrrolidin- 2-yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid hydrochloride hydrate.

Into a solution of 2- (4- {3- [3- (2-carboxyphenoxy) -2- propylphenoxy] propoxy} -5-ethyl-2-hydroxyphenyl ) pyrrolidine- 1-carboxylic acid tert-butyl ester lithium salt hydrate (0.100 g, 0.16 mmol) in anhydrous diethyl ether (5 mL) was bubbled gaseous HCl. The resulting mixture was allowed to stir for 1 h. The mixture was concentrated in vacuo. Chromatography (SCX cation exchange resin, 1:1 tetrahydrofuran/methanol to dilute ammonia/methanol) of the residue provided a tan solid. This material was dissolved in ether and treated with gaseous HCl. This mixture was concentrated in vacuo to provide 48 mg (52%) of the title

1 compound. H NMR (DMSO-dg) δ 12.80 (bs, IH) , 10.12 (s, IH) , 9.34 (bs, IH) , 8.36 (bs, IH) , 7.79 (dd, J = 9, 2 Hz, IH) , 7.47 (dt, J = 8, 2 Hz, IH) , 7.17 (t, J = 8 Hz, IH) , 7.12 (d, J = 9 Hz, IH) , 7.07 (s, IH) , 6.80 (d, J = 9 Hz, IH) , 6.78 (d, J = 9 Hz, IH) , 6.58 (s, IH) , 6.35 (d, J = 9 Hz, IH) , 4.56 (m, IH) , 4.20 (t, J = 6 Hz, 2H) ; 4.11 (t, J = 6 Hz, 2H) , 3.25 (m, 2H) , 2.50 (m, 5H) , 1.90-2.60 (m, 5H) , 1.44

(hextet, J = 8 Hz, 2H) , 1.08 (t, J = 7 Hz, 3H) , 0.82 (t, J =

+ 7 Hz, 3H) ; TOS MS ES exact mass calculated for C₃₁H₃₈NO

(p + 1) : m/z = 520.2699. Found: 520.2672

Example 17 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-thiophen-3-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoiσ acid hydrate.

Known compound:

Sawyer et al . , J. Med. Chem. 1995, 38, 4411.

A. Preparation of 3- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] thiophene. A mixture of 4- (benzyloxy) -5-bromo- 2- (3-chloropropoxy) ethylbenzene (1.90 g, 5.30 mmol), 3- thiopheneboronic acid (2.00 g, 15.9 mmol), tetrakis (triphenylphosphine)palladium(O) (312 mg, 0.270 mmol) , 2 M aqueous sodium carbonate solution (4 mL) , and n- propanol (4 mL) in toluene (16 mL) was refluxed for 4 h. The mixture was cooled to room temperature, diluted with diethyl ether, washed once with water and once with saturated sodium chloride solution. The organic layer was dried (magnesium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 1.54 g (80%) of the title

1 product as a white solid: mp 65-67 °C. H NMR (CDC1₃) δ

7.58 (d, J = 2.8 Hz, IH) , 7.49 (d, J = 5.2 Hz, IH) , 7.45- 7.30 (m, 7H) , 6.62 (s, IH) , 5.13 (s, 2H) , 4.14 (t, J = 5.8 Hz, 2H) , 3.81 (t, J = 6.3 Hz, 2H) , 2.66 (q, J = 7.5 Hz, 2H) , 2.29 (quintet, J = 6.0 Hz, 2H) , 1.24 (t, J = 7.5 Hz, 3H) ; MS

-1 FD m/e 386 (p) ; IR (CHCI3, cm ) 2969, 1613, 1501, 1138.

Anal. Calcd for C₂₂H₂₃0₂C1S: C, 68.29; H, 5.99. Found: C, 68.53; H, 6.00.

Known compound:

Sawyer et al.,

J. Med. Chem. 1995, 38, 4411.

B. Preparation of 2- [2-propyl-3- [3- [5- (benzyloxy) -2-ethyl- 4- (thiophen-3-yl)phenoxy]propoxy]phenoxy]benzonitrile.

A mixture of 4- (benzyloxy) -2- (3-chloropropoxy) -5- (thiophen- 3-yl) ethylbenzene (1.25 g, 3.23 mmol), 3- (2-cyanophenoxy) -2- propylphenol (0.82 g, 3.2 mmol), potassium iodide (0.21 g, 1.3 mmol), potassium carbonate (1.12 g, 8.08 mmol), and methyl sulfoxide (2 mL) in 2-butanone (10 mL) was refluxed for 60 h. The mixture was cooled to room temperature, diluted with ether, and washed with water. The organic layer was dried (magnesium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 1.31 g (67%) of

1 the title product as a colorless oil. H NMR (CDC1₃) δ 7.66 (d, J = 7.8 Hz, IH) , 7.57 (d, J = 2.9 Hz, IH) , 7.48 (d, J = 5.2 Hz, IH) , 7.45-7.25 (m, 8H) , 7.20 (t, J = 8.2 Hz, IH) ,

7.10 (t, J = 8.1 Hz, IH) , 6.82 (d, J = 8.3 Hz, IH) , 6.77 (d, J = 8.6 Hz, IH) , 6.64 (s, IH) , 6.63 (d, J = 6.4 Hz, IH) ,

5.11 (s, 2H) , 4.26 (t, J = 6.0 Hz, 2H) , 4.22 (t, J = 6.0 Hz, 2H) , 2.65 (m, 4H) , 2.36 (quintet, J = 5.9 Hz, 2H) , 1.58

(hextet, J = 7.5 Hz, 2H) , 1.24 (t, J = 7.5 Hz, 3H) , 0.95 (t,

-1 J = 7.3 Hz, 3H) ; MS FD m/e 603 (p) ; IR (CHCI3, cm ) 2967,

2250, 1613, 1501. Anal. Calcd for C₃₈H₃₇N0₄S: C, 75.59; H, 6.18; N, 2.32. Found: C, 74.65; H, 6.21; N, 2.57.

C. Preparation of 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (thiophen-3-yl)phenoxy]propoxy]phenoxy]benzonitrile.

To a solution of 2- [2-propyl-3- [3- [5- (benzyloxy) -2-ethyl-4- ( thiophen-3-yl)ρhenoxy]propoxy]phenoxy]benzonitrile (900 mg, 1.49 mmol) in methylene chloride (25 mL) cooled to -78 °C was added 1 M boron tribromide solution in methylene chloride (2.99 mL, 2.99 mmol) over 2 min. The resulting deep violet solution was stirred for 30 min and allowed to warm to room temperature. The mixture was diluted with water and shaken. The organic layer was separated, dried (magnesium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 25% ethyl acetate, 75% hexane) provided 400 mg (52%) of the title product as a colorless

1 oil. H NMR (CDC1₃) δ 7.84 (d, J = 4.8 Hz, IH) , 7.71 (d, J =

4.9 Hz, IH) , 7.66 (d, J = 7.7 Hz, IH) , 7.62 (s, IH) , 7.42 (t, J = 7.1 Hz, IH) , 7.27 (t, J = 6.6 Hz, IH) , 7.20 (s, IH) , 7.08 (t, J = 6.9 Hz, IH) , 6.85 (s, IH) , 6.89 (d, J = 8.1 Hz, IH) , 6.74 (d, J = 8.5 Hz, IH) , 6.60 (d, J = 7.6 Hz, IH) , 4.71 (s, IH, -OH), 4.26 (t, J = 6.0 Hz, 4H) , 2.72 (q, J = 7.4 dHz, 2H) , 2.59 (t, J = 7.3 Hz, 2H) , 2.39 (quintet, J = 6.1 Hz, 2H) , 1.54 (hextet, J = 7.7 Hz, 2H) , 1.25 (t, J = 7.5 Hz, 3H) , 0.91 (t, J = 7.4 Hz, 3H) . D. Preparation of 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (thiophen-3-yl)phenoxy]propoxy]phenoxy]benzoic acid hydrate,

A solution of 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4-

( thiophen-3-yl) phenoxy] propoxy] phenoxy] benzonitrile (400 mg, 0.780 mmol) in 2 : 1 methanol/water (6 mL) was treated with 12.5 M aqueous sodium hydroxide (4.0 mL) at reflux for 36 h. The mixture was cooled to room temperature, diluted with water, and extracted once with diethyl ether. The aqueous layer was acidified with concentrated hydrochloric acid and extracted twice with methylene chloride. The combined methylene chloride layers were dried (magnesium sulfate) , filtered, and concentrated in vacuo to provide a tan solid:

1 mp 90-95 °C (dec). H NMR (CDCI3) δ 8.24 (d, J = 7.8 Hz,

IH) , 7.47 (d, J = 5.0 Hz, IH) , 7.44 (t, J = 8.6 Hz, IH) , 7.36 (d, J = 3 Hz, IH) , 7.24 (d, J = 4.9 Hz, IH) , 7.19 (m, 2H) , 7.09 (s, IH) , 6.84 (d, J = 8.0 Hz, IH) , 6.73 (d, J = 8.3 Hz, IH) , 6.64 (d, J = 8.0 Hz, IH) , 6.55 (s, IH) , 5.38 (bs, IH, -OH), 4.26 (t, J = 6.2 Hz, 2H) , 4.21 (t, J = 7.1

Hz, 2H) , 2.60 (m, 4H) , 2.36 (quintet, J = 5.8 Hz, 2H) , 1.51 (hextet, J = 7.1 Hz, 2H) , 1.19 (t, J = 7.5 Hz, 3H) , 0.90 (t, -1 J = 7.4 Hz, 3H) ; MS FD m/e 532 (p) ; IR (KBr, cm ) 3200

(br) , 2961, 1697, 1457, 1110. Anal. Calcd for C₃₁H₃₂0gS .

H₂0: C, 67.62; H, 6.22. Found: C, 67.34; H, 5.87.

The cancers which may be treated using the present method, are those which are amenable to radiation therapy. These include cancers such as Prostate Cancer, Colon Cancer, Breast Carcinoma, Bladder Carcinoma, Colorectal Carcinoma, Esophageal Carcinoma, Gastric Carcinoma, Germ Cell Carcinoma e.g. Testicular Cancer, Gynecologic Carcinoma, Lymphoma - Hodgkin's, Lymphoma - Non-Hodgkin' s, Malignant Melanoma, Multiple Myeoma, Neurologic Carcinoma, Brain Cancer, Non Small Cell Lung Cancer, Pancreatic Carcinoma, Prostate Carcinoma, Ewings Sarcoma, Osteosarcoma, Soft Tissue Sarcoma, Pediatric Malignancies and the like.

The types of radiation that may be used to treat cancer according to the present invention include X-rays, gamma rays, high-energy electrons and High LET (Linear Energy Transfer) radiation, such as protons, neutrons and alpha particles. The ionizing radiation is employed by techniques well-known to those skilled in the art. For example, X-rays and gamma rays are applied by external and/or interstitial means from linear accelerators or radioactive sources. High energy electrons can be produced by linear accelerators.

High LET radiation is also produced by linear accelerators and can also be applied from radioactive sources implanted interstitially .

The compounds or formulations of the present invention may be administered by the oral and rectal routes, topically, parenterally, e.g., by injection and by continuous or discontinuous intra-arterial infusion, in the form of, for example, tablets, lozenges, sublingual tablets, sachets, cachets, elixirs, gels, suspensions, aerosols, ointments, for example, containing from 1 to 10% by weight of the active compound in a suitable base, soft and hard gelatin capsules, suppositories, injectable solutions and suspensions in physiologically acceptable media, and sterile packaged powders adsorbed onto a support material for making injectable solutions. Advantageously for this purpose, compositions may be provided in dosage unit form, preferably each dosage unit containing from about 5 to about 500 mg (from about 5 to 50 mg in the case of parenteral or inhalation administration, and from about 25 to 500 mg in the case of oral or rectal administration) of a compound of Formula I. Dosages from about 0.5 to about 300 mg/kg per day, preferably 0.5 to 20 mg/kg, of active ingredient may be administered although it will, of course, readily be understood that the amount of the compound or compounds of Formula I actually to be administered will be determined by a physician, in the light of all the relevant circumstances including the condition to be treated, the choice of compound to be administered and the choice of route of administration and therefore the above preferred dosage range is not intended to limit the scope of the present invention in any way.

The leukotriene (LTB4) antagonist formulations of the present invention normally will consist of at least one compound selected from the group consisting of compounds of Formula A, Formula I and Formula II mixed with a carrier, or diluted by a carrier, or enclosed or encapsulated by an ingestible carrier in the form of a capsule, sachet, cachet, paper or other container or by a disposable container such as an ampoule. A carrier or diluent may be a solid, semi- solid or liquid material which serves as a vehicle, excipient or medium for the active therapeutic substance. Some examples of the diluents or carrier which may be employed in the pharmaceutical compositions of the present invention are lactose, dextrose, sucrose, sorbitol, mannitol, propylene glycol, liquid paraffin, white soft paraffin, kaolin, fumed silicon dioxide, microcrystallme cellulose, calcium silicate, silica, polyvinylpyrrolidone, cetostearyl alcohol, starch, modified starches, gum acacia, calcium phosphate, cocoa butter, ethoxylated esters, oil of theobroma, arachis oil, alginates, tragacanth, gelatin, syrup, methyl cellulose, polyoxyethylene sorbitan monolaurate, ethyl lactate, methyl and propyl hydroxybenzoate, sorbitan trioleate, sorbitan sesquioleate and oleyl alcohol and propellants such as trichloromonofluoromethane, dichlorodifluoromethane and dichlorotetrafluoroethane. In the case of tablets, a lubricant may be incorporated to prevent sticking and binding of the powdered ingredients in the dies and on the punch of the tableting machine. For such purpose there may be employed for instance aluminum, magnesium or calcium stearates, talc or mineral oil.

Preferred pharmaceutical forms of the present invention are capsules, tablets, suppositories, injectable solutions, creams and ointments. Especially preferred are formulations for inhalation application, such as an aerosol, and for oral ingestion. Pharmaceutical Compositions of the Invention

The pharmaceutical composition of the invention comprises as essential ingredients: (a) an LTB4 antagonist, and

(b) an anti-cancer agent. When the pharmaceutical composition of the invention is prepared in injectable form it is a composition comprising as ingredients: (a) an LTB4 antagonist,

(b) an anti-cancer agent, and

(c) an injectable liquid carrier. Pharmaceutically acceptable carriers are those well known in the medical arts, such as sterile water, sterile water containing saline, and sterile water containing sugars and/or saline.

a. Ratio and Amount of Ingredients in the Composition of the Invention The essential ingredients (a) an LTB4 antagonist and (b) anti-cancer compound are present in the formulation in such proportion that a dose of the formulation provides a pharmaceutically effective amount of each ingredient to the patient being treated. Typically, the weight ratio of LTB4 antagonist to anti-cancer agent 1:100 to 100 to 1, preferable from 10:1 to 1:10 and most preferable from 1:4 to 4:1.

The following formulation examples illustrate the types of formulations of the leukotriene (LTB4) antagonists which may be employed in a method of the present invention. The examples may employ as active compounds any of the compounds of this invention. The examples are illustrative only and are not intended to limit the scope of the invention in any way.

FORMULATION EXAMPLE 1

Hard gelatin capsules are prepared using the following ingredients : Quantity

(mg/capsule)

3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4-carboxy- phenoxy) phenyl) propanoic acid 250

Starch 200

Magnesium stearate 10

The above ingredients are mixed and filled into hard gelatin capsules in 460 mg quantities.

FORMULATION EXAMPLE 2

A tablet is prepared using the ingredients below: Quantity

(mg/capsule) 1- (4- (Carboxymethoxy) phenyl) -1- (1H- tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) exane 250

Cellulose, microcrystallme 400

Silicon dioxide, fumed 10 Magnesium stearate 5

The components are blended and compressed to form tablets each weighing 665 mg.

FORMULATION EXAMPLE 3

An aerosol solution is prepared containing the following components:

Weight %

3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4-fluorophenyl) -5-hydroxyphenoxy]propoxy] -

9H-xanthene] ] propanoic acid 0.25

Ethanol 30.00

Propellant 11 10.25

(trichlorofluoromethane)

Propellant 12 29.75

(Dichlorodifluoromethane)

Propellant 114 29.75

(Dichlorotetrafluoroethane)

The active compound is dissolved in the ethanol and the solution is added to the propellant 11, cooled to -30°C. and transferred to a filling device. The required amount is then fed to a container and further filled with the pre-mixed propellants 12 and 114 by means of the cold-filled method or pressure-filled method. The valve units are then fitted to the container. FORMULATION EXAMPLE 4

Tablets each containing 60 mg of active ingredient are made up as follows:

2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) henoxy] propoxy] phenoxy] - benzoic acid sodium salt 60 mg

Starch 45 mg

Microcrystallme cellulose 35 mg Polyvinylpyrrolidone 4 mg

(as 10% solution in water)

Sodium carboxymethyl starch 4.5 mg Magnesium stearate 0.5 mg

Talc 1 mg

Total 150 mg

The active ingredient, starch and cellulose are passed through a No. 45 mesh (355 μm) U.S. sieve and mixed thoroughly. The solution of polyvinylpyrrolidone is mixed with the resultant powders which are then passed through a No. 14 mesh (1.4 mm) U.S. sieve. The granules so produced are dried at 50-60°C and passed through a No. 18 mesh (1.00 mm) U.S. sieve. The sodium carboxymethyl starch, magnesium stearate and talc, previously passed through a No.

60 mesh (250 μm) U.S. sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets each weighing 150 mg. FORMULATION EXAMPLE 5

Capsules each containing 80 mg of medicament are made as follows:

5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] - phenyl] -4-pentynoic acid 80 mg

Starch 59 mg

Microcrystallme cellulose 59 mg

Magnesium stearate 2 mg

Total 200 mg

The active ingredient, cellulose, starch and magnesium stearate are blended, passed through a No . 45 mesh

(355 μm) U.S. sieve, and filled into hard gelatin capsules in 200 mg quantities.

FORMULATION EXAMPLE 6

Suppositories each containing 225 mg of active ingredient are made as follows:

3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3, 4-tetrahydronaphthalen-l (2H) - one) propanoic acid 225 mg

Unsaturated or saturated fatty acid glycerides to 2,000 mg

The active ingredient is passed through a No. 60 mesh

(250 μm) U.S. sieve and suspended in the fatty acid glycerides previously melted using the minimum heat necessary. The mixture is then poured into a suppository mold of nominal 2 g capacity and allowed to cool.

FORMULATION EXAMPLE 7

Suspensions each containing 50 mg of medicament per 5 mL dose are made as follows:

2- [2-Propyl-3- [3- [2-ethyl-4- (4-fluorophenyl) -

5-hydroxyphenoxy] propoxy] phenoxy] benzoic acid 50 mg

Sodium carboxymethyl cellulose 50 mg

Sugar 1 g Methyl paraben 0.05 mg

Propyl paraben 0.03 mg

Flavor q.v.

Color q.v.

Purified water to 5 mL

The medicament is passed through a No . 45 mesh (355 μm) U.S. sieve and mixed with the sodium carboxymethylcellulose, sugar, and a portion of the water to form a suspension. The parabens, flavor and color are dissolved and diluted with some of the water and added, with stirring. Sufficient water is then added to produce the required volume. FORMϋLATION EXAMPLE 8

Hard gelatin capsules are prepared using the following ingredients:

Quantity (mg/capsule)

1- (4-amino-5-methyl-2-oxo-lH- pyrimidin-1-yl) -2-desoxy- 250 2 ' , 2 ' -difluororibose

Starch dried 200

Magnesium stearate 10

The above ingredients are mixed and filled into hard gelatin capsules in 460 mg quantities.

FORMULATION EXAMPLE 9

A tablet formula is prepared using the ingredients below:

Quantity (mg/tablet)

1- (2-oxo-4-amino-lH-pyrimidin- 1-yl) -2-desoxy-2' , 2 ' -difluoro- 250 ribose

Cellulose, microcrystallme

400

Silicon dioxide, fumed 10

Stearic acid 5

The components are blended and compressed to form tablets each weighing 665 mg.

FORMULATION EXAMPLE 10

An aerosol solution is prepared containing the following components:

Weight %

l-(2,4-dioxo-lH,3H-pyrimidin- 1-yl) -2-desoxy-2 ' , 2 ' -difluororibose 0.25 Ethanol 29.75 Propellant 22 70.00 (Chlorodifluoromethane) The active compound is mixed with ethanol and the mixture added to a portion of the propellant 22, cooled to - 30. degree. C. and transferred to a filling device. The required amount is then placed in a stainless steel container and diluted with the remainder of the propellant. The valve units are then fitted to the container.

FORMULATION EXAMPLE 11

Tablets each containing 60 mg of active ingredient are made up as follows:

1- (4-amino-2-oxo-lH-pyrimidin-

1-yl) -2-desoxy-2' , 2 ' -difluororibose 60 mg

Starch 45 mg

Microcrystallme cellulose 35 mg

Polyvinylpyrrolidone 4 mg

(as 10% solution in water)

Sodium carboxymethyl starch

4.5 mg Magnesium stearate 0.5 mg

Talc 1 mg

The difluoronucleoside starch and cellulose are passed through a No. 45 mesh U.S. sieve and mixed thoroughly. The solution of polyvinylpyrrolidone is mixed with the resultant powders which are then passed through a No. 14 mesh U.S. sieve. The granules so produced are dried at 50. degree.- 60. degree. C. and passed through a No . 18 mesh U.S. sieve. The sodium carboxymethyl starch, magnesium stearate and talc, previously passed through a No . 60 mesh U.S. sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets each weighing 150 mg.

FORMULATION EXAMPLE 12

Capsules each containing 80 mg of medicament are made as follows :

1- (4-amino-2-oxo-lH-pyrimidin- 1-yl) -2-desoxy-2 ' , 2 ' -difluor- oxylose 80 mg Starch 59 mg Microcrystallme cellulose

59 mg

Magnesium stearate 2 mg

The active ingredient, cellulose, starch and magnesium stearate are blended, passed through a No . 45 mesh U.S. sieve, and filled into hard gelatin capsules in 200 mg quantities .

FORMULATION EXAMPLE 13

Suppositories each containing 225 mg of nucleoside are made as follows:

1- (2, 4-dioxo-lH, 3H-pyrimidin- 1-yl) -2-desoxy-2 ' , 2 ' -difluororibose 225 mg Saturated fatty acid 2 g glycerides to

The nucleoside is passed through a No. 60 mesh U.S. sieve and suspended in the saturated fatty acid glycerides previously melted using the minimum heat necessary. The mixture is then poured into a suppository mold of nominal 2 g capacity and allowed to cool.

FORMULATON EXAMPLE 14

Suspensions each containing 50 mg of medicament per 5 ml dose are made as follows:

1- (4-amino-5-methyl-2-oxo-lH- pyrimidin-1-yl) -2-desoxy-2 , 2- difluororibose 50 mg

Sodium carboxymethyl

Cellulose 50 mg

Syrup 1.25 ml

Benzoic acid solution 0.10 ml

Flavor q.v.

Color q.v.

Purified water to 5 ml

FORMULATION EXAMPLE 15 An intravenous formulation is prepared as follows

1- (4-amino-2-oxo-lH-pyrimidin- 1-yl) -2-desoxy-2 ' , 2 ' -difluoro ribose 100 mg isotonic saline 1000 ml

The solution of the above ingredients is administered intravenously at a rate of 1 ml/minute to a mammal in need of treatment from susceptible neoplasms. FORMULATION EXAMPLE 16

Hard gelatin capsules are prepared using the following ingredients : Quantity

(mg/capsule) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4-carboxy- phenoxy) phenyl) propanoic acid 250

2 ' , 2 ' -Diflouro-2 ' -deoxycytidine monohydrochloride

250

Starch 200

Magnesium stearate 10

The above ingredients are mixed and filled into hard gelatin capsules in 710mg quantities.

FORMULATION EXAMPLE 17

A tablet is prepared using the ingredients below: Quantity (mg/capsule)

1- (4- (Carboxymethoxy) phenyl) -1- (1H- tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane

250 2 ' , 2 ' -Difluoro-2 ' -deoxycytidine monochloride 250

Cellulose, microcrystallme 400

Silicon dioxide, fumed 10

Magnesium stearate 5

The components are blended and compressed to form tablets each weighing 915 mg.

FORMULATION EXAMPLE 18

An aerosol solution is prepared containing the following components:

Weight % 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4-

(4-fluorophenyl) -5-hydroxyphenoxy] propoxy] - 9H-xanthene] ] propanoic acid 0.25

2 ' , 2 ' -difluoro-2 ' -deoxycytidine monohydrochloride 0.25

Ethanol 30.00

Propellant 11 10.00

(trichlorofluoromethane)

Propellant 12 29.75 (Dichlorodifluoromethane)

Propellant 114 29.75

(Dichlorotetrafluoroethane)

The active compound is dissolved in the ethanol and the solution is added to the propellant 11, cooled to -30°C. and transferred to a filling device. The required amount is then fed to a container and further filled with the pre-mixed propellants 12 and 114 by means of the cold-filled method or pressure-filled method. The valve units are then fitted to the container. FORMULATION EXAMPLE 19

Tablets each containing 60 mg of active ingredient are made up as follows:

2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] - benzoic acid sodium salt 60 mg

2 ' , 2 ' -difluoro-2 ' deoxycytidine monohydrochloride 60 mg

Starch 45 mg

Microcrystallme cellulose 35 mg

Polyvinylpyrrolidone 4 mg

(as 10% solution in water)

Sodium carboxymethyl starch 4 . 5 g

Magnesium stearate 0 . 5 mg

Talc 1 mg

Total 210 mg

The active ingredient, starch and cellulose are passed through a No. 45 mesh U.S. sieve (355 μm) and mixed thoroughly. The solution of polyvinylpyrrolidone is mixed with the resultant powders which are then passed through a No. 14 mesh U.S. sieve (1.4 mm). The granules so produced are dried at 50-60° and passed through a No . 18 mesh U.S. sieve (1.00 mm). The sodium carboxymethyl starch, magnesium stearate and talc, previously passed through a No. 60 mesh U.S. sieve (250 μm) , are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets each weighing 210 mg. FORMULATION EXAMPLE 20

Capsules each containing 80 mg of medicament are made 5 as follows:

5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] - phenyl] -4-pentynoic acid 80 mg

10

2 ' , 2 ' -difluoro-2 ' deoxycytidine monohydrochloride 80 mg

15 Starch 59 mg

Microcrystallme cellulose 59 mg

Magnesium stearate 2 mg

-20

Total 280 mg

The active ingredient, cellulose, starch and magnesium stearate are blended, passed through a No. 45 mesh U.S. 25 sieve (355 μm) , and filled into hard gelatin capsules in 280 mg quantities.

FORMULATION EXAMPLE 21

Suppositories each containing 225 mg of active ingredient are made as follows:

3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3, 4-tetrahydronaphthalen-l (2H) - one) propanoic acid 225 mg

2 ' , 2 ' -difluoro-2 ' -deoxycytidine monochloride 225 mg

Unsaturated or saturated fatty acid glycerides to 2,000 mg

The active ingredient is passed through a No. 60 mesh U.S. sieve (250 μm) and suspended in the fatty acid glycerides previously melted using the minimum heat necessary. The mixture is then poured into a suppository mold of nominal 2 g capacity and allowed to cool.

FORMULATION EXAMPLE 22

Suspensions each containing 50 mg of medicament per 5 mL dose are made as follows:

2- [2-Propyl-3- [3- [2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy] propoxy] phenoxy] benzoic acid 50 mg 2 ' , 2 ' -difluoro-2 ' -deoxycytidine monohydrochloride 50 mg

Sodium carboxymethyl cellulose 50 mg Sugar 1 g

Methyl paraben 0.05 mg

Propyl paraben 0.03 mg

Flavor q.v.

Color q.v. Purified water to 5 mL

The medicament is passed through a No . 45 mesh U.S. sieve (355 μm) and mixed with the sodium carboxymethylcellulose, sugar, and a portion of the water to form a suspension. The parabens, flavor and color are dissolved and diluted with some of the water and added, with stirring. Sufficient water is then added to produce the required volume.

Gemcitabine is most often given in the form of the hydrochloride salt and it is preferred that it be administered by intravenous infusion. The dose is generally in the range of 750 to 1250 mg/m² infused within 30 minutes to an hour . The leukotriene (LTB4) antagonists may be administered along with 2 ', 2 ' -difluoronucleoside anti-cancer agent. In this case, they are formulated together in a formulation suitable for intravenous administration. On the other hand, it is often preferred to administer the molecules separately since the patient may be sensitive to one or the other drug. If the molecules are administered separately from each other or the radiation dose, they should be administered within a therapeutically effective interval. Therapeutically effective interval is a period of time beginning when one of either (a) the leukotriene (LTB4) antagonists antagonist or (b) the anti-cancer agent is administered to a human and ending at the limit of the beneficial effect in the treatment of cancer of the combination of (a) and (b) . One might wish to reduce the dosage of the drug to which the patient is sensitive without reducing the dosage of the other drug. This is particulary true with 2', 2'- difluoronucleoside anti-cancer agent where a reduction in granulocyte counts or platelet counts which suggest the need for reducing the dosage of the drug while the leukotriene (LTB4) antagonist could be administered at the normal dosage. The leukotriene (LTB4) antagonists may be administered during the course of radiation. However, it is preferred that the leukotriene antagonists be administered for some time before radiation has begun. Such administration allows for an effective level of the leukotriene antagonists to be established in the tissue before radiation therapy is undertaken. It is preferred to begin the administration of leukotriene antagonists 1-3 days before the beginning of the radiation therapy, and continue with throughout the course of the radiation therapy. Assay Example 1

The murine Lewis lung carcinoma was implanted in male C57B1 mice and the tumor-bearing animals were treated with the compound of Formula IV alone or along with fractionated radiation therapy. Specifically, Lewis lung tumor cells prepared from a brie of donor tumors (1 x 10⁶ cells) were implanted in a hind-leg of male C57B1 mice ( Charles River) . Fractionated radiation therapy was delivered locally to the tumor-bearing limb in five fractions of 200, 300, or 400 rads to total doses of 1000, 1500, or 2000 rads (GammaCell 40 irridiator, MSD Nordion Inc., Ottawa, ON, Canda, 137 cesium source) once per day on days 7 through 11 post tumor cell implantation. This radiation was administered alone or along with the compound of Formula IV. Treatment with the compound of Formula IV (100 mg/kg) was administered orally on day 4 post tumor cell implantation and continued daily until day 21.

Each treatment group as well as a group of untreated control animals consisted of five animals per group. Tumor response was monitored by tumor volume measurement performed twice per week over the course of 31 days. Lung metastases were counted from two animals per group. Body weights were determined as a general measure of toxicity.

The data was analyzed by determining the mean tumor volume for each treatment group over the course of the experiment and calculating the tumor growth delay as the difference in days for the treatment versus the control tumors to reach a volume of 500 mm³. Table 1

Lewis Lung Test Results Growth Delay of Lung Tumor'¹¹

Primary lewis lung carcinoma Formula IV = the LTB4 antagonist, 2- [2-propyl-3- [3- [2- ehtyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid GEM = gemcitabine hydrochloride, a 2 ' , 2 ' -difluoro-2 ' - deoxycytidine; 2 ' -Deoxy-2 ' , 2 ' -difluorocytidine; molecular formula CgHnF₂N0 ; Chemical Abstract Registry Number 95058-81-4, a product of Eli Lilly and Company Radiation = five fractions of dose Rads from a GammaCell 40 irradiator, MSD Nordion, Inc.

Dose Formula IV = milligrams per kilogram mouse body weight

Dose GEM = = milligrams per kilogram mouse body weight Dose Rads = rads per fraction Rad = 0.01 gray = 0.01 joule per kilogram TGD = average tumor growth delay in days sem = standard error of the mean

Table 2

Lewis Lung Test Results Reduction in Mean Number of Lung Metastases

MNLM = Mean number of lung metastases

Claims

We Claim:

1. A method of treating a human patient suffering from cancer which comprises administering to said patient ionizing radiation in conjunction with an effective amount of a 2 ', 2 ' -difluoronucleoside anti-cancer compound and an effective amount of a leukotriene LTB4 inhibitor selected from the group consisting of Formula I and Formula II, or a pharmaceutically acceptable base addition salt thereof.

2. Use of a leukotriene (LTB4) antagonist in combination with a 2 ', 2 ' -difluoronucleoside anti-cancer agent for the manufacture of a medicament for administration in combination with irradation with high energy radiation for the treatment of cancer.

3. The use according to claim 2 wherein the anti- cancer compound is a therapeutically effective amount of a compound represented by the formula:

where :

R¹ is hydrogen;

R² is a base defined by one of the formulae:

X is C-R⁴ ;

R³ is hydrogen;

R⁴ is hydrogen, Cι-C alkyl, bromo, fluoro, chloro or iodo; and pharmaceutically acceptable salts thereof.

4. The use according to claim 3 wherein R2 is the base defined by the formula:

5. The use according to claim 4 wherein the anti- cancer agent is selected from the group consisting of the following compounds or a pharmaceutically acceptable salt therof :

(i) 1- (4-amino-2-oxo-lH-pyrimidin-l-yl) -2-desoxy-2 ' , 2 ' - difluororibose, (ii) 1- (4-amino-2-oxo-lH-pyrimidin-l-yl) -2-desoxy- 2 ' , 2 ' -difluoroxylose,

(iii) 1- (2 , 4-dioxo-lH, 3H-pyrimidin-l-yl) -2-desoxy- 2 ', 2 ' -difluororibose, and

(iv) 1- (4-amino-5-methyl-2-oxo-lH-pyrimidin-l-yl) -2- desoxy-2 ', 2 ' -difluororibose.

6. The use according to claim 5 wherein the 2 ' , 2 ' ^■ difluornucleoside is gemcitabine HCl, namely 2 ' -deoxy-2 ' , 2 ' ^■ difluorocytidine monohydrochloride (β-isomer)or l-(4-amino- 2-oxo-lH-pyrimidin-l-yl) -2-desoxy-2 ' ,^'2 ' -difluororibose .

7. The use according to claim 2 or 3 or 4 or 5 or 6 wherein the leukotriene (LTB4) antagonist is represented by the formula (I)

(1) wherein:

X is selected from the group consisting of,

(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; and

(ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i);

Yi is a bond or divalent linking group containing 1 to 9 atoms ;

Y2 and Y3 are divalent linking groups independently selected from -CH₂~, -0-, or -S-; Z is an Acidic Group;

Rl is Cι-Cχo alkyl, aryl, C3-C8 cycloalkyl, C2-C10 alkenyl, C2-C10 alkynyl, Cg-C20 aralkyl, C5-C20 alkaryl, Cχ-Cιo haloalkyl, C5-C20 aryloxy, or Cχ-Cιo alkoxy;

R2 is hydrogen, halogen, Cχ-Cιo haloalkyl, Cχ-C o alkoxy, Cχ-Cχo alkyl, C3-C8 cycloalkyl, Acidic Group, or - (CH2) 1-7- (Acidic Group);

R3 is hydrogen, halogen,

alkyl, aryl, Cχ-Cιo haloalkyl, C^-CXQ alkoxy, C5-C20 aryloxy, or C3-C8 cycloalkyl;

R4 is C1-C alkyl, C3-C4 cycloalkyl, - (CH2) 1-7- (C3-C4 cycloalkyl), C2-C4 alkenyl, C2-C4 alkynyl, benzyl , or aryl ; and

n is 0, 1, 2, 3, 4, 5, or 6 ;

or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof .

8. The use according to claim 7 wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following formulae:

0

where RIO is a radical selected from hydrogen or

C -C4 alkyl; and Rll is a radical selected from hydrogen, halo, Ci-Cio alkyl, Cχ-Cιo haloalkyl, Cχ-Cχo alkoxy, aryl, or C6-C20 aryloxy.

9. The use according to claim 8 wherein the Rl, R2 , R3 and R4 groups for substitution in formula (I) are selected from the following variables coded R01 thru R16

and;

the Yl, Y2 , and Y3 groups for substitution in formula (I) are selected from the following variables coded YOl thru Y27:

and;

the X and Z groups and the n variable for substitution in formula (I) are selected from the following variables coded XZnOl thru XZn2 :

10. A use according to claim 2 or 3 or 5 or 6 wherein the leukotriene B4 antagonist is described by formula (II) :

wherein;

X2 is a heterocyclic radical selected from,

or

R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and

R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.

Z2 is the Acidic Group selected from carboxyl, tetrazolyl, or N-sulfonamidyl;

or a salt, solvate or prodrug thereof.

11. The use according to claim 10, wherein the leukotriene antagonist is a compound selected from the following:

or an acid, salt, solvate or prodrug derivative thereof.

12. The use according to claim 11 wherein the leukotriene antagonist is a compound selected from the following:

or an acid, salt, solvate or prodrug derivative thereof.

13. The use according to claim 2 or 3 or 4 or 5 or 6 wherein the leukotriene (LTB4) antagonist is represented by a compound of the structure (Formula A) :

Formula A

or a pharmaceutically acceptable base addition salt thereof, wherein:

Rl' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2~substitutedphenyl ; each R2 ' and R3 ' are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1-C4 alkoxy, (C1-C4 alkyl) -(0)g S-, trifluoromethyl, or di- (C1-C3 alkyl)amino; X' is -0-, -S-, -C(=0), or -CH2-; Y' is -0- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C=C-; Z' is a straight or branched chain C1-C10 alkylidenyl; A' is a bond, -0-, -S-, -CH=CH- , or -CR_aR£_>-' where R_a and Rjh are each independently hydrogen, C1-C5 alkyl, or R7- substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring;

R4 / is Rg , or one of the following formulae;

wherein: each R6 is independently -COOH, 5-tetrazolyl, -

CON⁽R₉) ' or -CONHS0₂Rlo; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-R6, -T-G-R6, (C1-C4 alkyl) -T- (C1-C4 alkylidenyl) -0-, or hydroxy;

R8 is hydrogen or halo; each R9 is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;

RlO is C1-C4 alkyl or phenyl; Rll is R2, -W-Rg, or -T-G-R6; each W is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0-, -NH-, -NHCO- , -C(=0)-, or (0)_g S-;

K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2; p is 0 or 1; and t is 0 or 1; provided when X is -O- or -S-, Y is not -0- ; provided when A is -0- or -S-, R4 is not Rβ ; and provided W is not a bond when p is 0.

14. The use of claim 13 wherein R4 ' is selected from the following formulae:

15. The use of claim 14 wherein R4 ' is:

16. The use according to claim 15 wherein said compound lected from the group (A) to (KKKK) consisting of:

A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) heptane;

B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- ( 3-fluorophenyl ) -5-hydroxyphenoxy) heptane;

C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- dimethylaminocarbonylbutyloxy) phenyl ) propionic acid;

D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

E) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutyloxy) phenyl ) propionic acid;

F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid;

G) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl ) butyloxy) phenyl ) propionic acid;

H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) - (1- butenyl) ) phenyl) propionate; I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl) ) phenyl) propionic acid;

J) 3- (2- (4- ( 2 -Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) phenyl ) propionic acid;

K) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) -6- methoxyphenyl) propionic acid;

L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionate;

M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid;

N) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- butyloxy) phenyl) propionic acid; 0) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- methylthiobutyloxy) phenyl) propionic acid;

P) 3- (2- (3- (2, 4-Di- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutoxy) phenyl ) propionic acid;

Q) 6-Methyl-6- (lH-tetrazol-5-yl) -11- (2-ethyl-4- ( 4-fluorophenyl) -5-hydroxyphenoxy) undecane;

R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; Ξ) N-Methanesulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide;

T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; U) 3- (2- (3- (2-Butyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; V) Ethyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionate;

W) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionic acid;

X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- (4- (methoxycarbonyl ) phenoxy) phenyl) propionate ;

Y) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- carboxyphenoxy) phenyl ) propionic acid; Z) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -4- (4- carboxyphenoxy) phenyl) propionic acid;

AA) 3,3-Dimethyl-3-(2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) propane ;

CC) 2-Methyl-2- (lH-tetrazol-5-yl) -3-hydroxy-3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) propane ;

DD) 3- (2- (3- (2-Bromo-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

EE) 3- (2- (3- (2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; FF) Methyl 3- (2-hydroxy-3- (4- methoxycarbonylbutyl) -6- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) propionate ; GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- ( 4- carboxybutyl ) dihydrocoumarin;

HH) 2-Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxy] phenol sodium salt;

II) 2- (4-Methylphenyl) -4-ethyl-5- [ 6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

JJ) 2- (3-Methylphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;

KK) 2- (2-Methylphenyl) -4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

LL) 2-(4-Methoxyphenyl)-4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt; MM) 2-(3-Methoxyphenyl)-4-ethyl-5- [ 6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;

NN) 2- (4-Trifluoromethylphenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

PP) 3- (5- (6- (4-Phenyl-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3,4 -tetrahydronaphthalen-1 (2H) - one) propanoic acid;

QQ) 3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3, 4-tetrahydronaphthalen-1 (2H) - one) propanoic acid; RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl-2, 3- dihydroinden-1 (2H) -one) propanoic acid; SS) 3,3-Dimethyl-5-(3- (2-carboxyethyl) -4- (3- (4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl ) -5-oxopentanoic acid; TT) 7- [3- [ (5-Ethyl-2-hydroxy[l,l' -biphenyl] -4- yl) oxy] propoxy] -3 , 4-dihydro-8-propyl-2H-l- benzopyran-2 -carboxylic acid;

UU) 8-Propyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-carboxylic acid;

W) 2- [3- [3- [ (5-Ethyl-2 -hydroxy [1,1' -biphenyl] - 4-yl) oxy] propoxy] -2 -propylphenoxy] propanoic acid;

WW) 2- (4-Chlorophenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt;

XX) 2- (3, 5-Dichlorophenyl)-4-ethyl-5- [6-methyl- 6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt; YY) 3-[2-[3-[ ( 5-Ethyl-2-hydroxy [1,1' -biphenyl ]-

4-yl) oxy] propoxy] -1-dibenzofuran] propanoic acid disodium salt;

ZZ) 7-Carboxy-9-oxo-3- [3- ( 2 -ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;

AAA) 2- [2-Propyl-3- [3- ( 2 -ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid sodium salt hemihydrate;

BBB) 3- [3- (2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl] -4- propanoic acid disodium salt monohydrate;

CCC) 5-Ethyl-4- [3- [2-propyl-3- [2- (2H-tetrazol-5- yl ) phenoxy] phenoxy] propoxy] [1,1' -biphenyl ] - 2-ol disodium salt sesquihydrate; DDD) 3-[4-[3-[ 3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ] propanoic acid sodium salt hemihydrate ;

EEE) 2-Fluoro-6- [2-propyl-3- [3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt;

FFF) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt;

GGG) 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate;

HHH) 3- [4- [9-0x0-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid;

III) 3- [2- [1- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid; JJJ) 2-Fluoro-6-[2-propyl-3-[3-[2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate; KKK) 4-Fluoro-2-[2-proρyl-3-[3-[2-ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; LLL) 2-[2-Propyl-3-[5-[2-ethyl-5-hydroxy-4-(4- fluorophenyl ) phenoxy] pentoxy] phenoxy] benzoic acid;

MMM) 2- [2-Propyl-3- [4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] butoxylphenoxy] benzoic acid sesquihydrate; NNN) 2- [2- (2-Methylpropyl) -3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;

OOO) 2- [2-Butyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid hydrate,- PPP) 2- [2- (Phenylmethyl) -3- [3- [2-ethyl-5-hydroxy-

4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; QQQ) 2-[2-Propyl-3-[3-[2-ethyl-5-hydroxy-4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] phenyla cetic acid;

RRR) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid;

SSS) 2- [ [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenyl ] methyl ] b enzoic acid;

TTT) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid;

UUU) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenylsulfinyl] benzoic acid;

VW) 2- [2-Proρyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfonyl ] benzoic acid hydrate;

WWW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- ( 4- fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenyl ] -4-pentynoic acid disodium salt 0.4 hydrate;

XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- ( 4-fluorophenyl ) -5 -hydroxyphenoxy) hexane ; YYY) 1- (4- (Carboxymethoxy) phenyl) -1- (lH-tetrazol- 5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane;

ZZZ) 1- (4- (Dimethylammocarbonylmethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane; AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;

BBBB) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) -2-methyl-E- propenoic acid;

CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl ) -1H- tetrazole;

DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- ( 4- carboxybutyloxy) phenyl) propionic acid;

EEEE) 5- [3- [4- (4-Fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-one; FFFF) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;

GGGG) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4- propylphenyl ) propanoic acid sodium salt;

HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] ropoxy} -3- propylphenyl ) propanoic acid;

IIII) 3- (3-{3-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl ) propanoic acid;

JJJJ) 3-{3-[3-(2-Ethyl-5- hydroxyphenyloxy) propoxy] -2- propylphenyl }propanoic acid disodium salt; and

KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid disodium salt hemihydrate.

17. The use according to claim 13 wherein the leukotriene (LTB4) antagonist is a compound of the structure (Formula B) :

Formula B

namely, 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxybenzoic acid, and the pharmaceutically acceptable salts thereof.

18. A method of treating a mammalian patient suffering from cancer which comprises administering to said patient ionizing radiation in conjunction with an effective amount of a 2 ', 2 ' -difluoronucleoside anti-cancer compound and an effective amount of a leukotriene LTB4 antagonist.

19. The method of claim 18 wherein the anti-cancer compound is a therapeutically effective amount of a compound represented by the formula:

where :

R¹ is hydrogen;

R² is a base defined by one of the formulae:

X is C-R⁴;

R³ is hydrogen;

R⁴ is hydrogen, Cι-C alkyl, bromo, fluoro, chloro or iodo; and pharmaceutically acceptable salts thereof.

20. The method of claim 19 wherein R2 is the base defined by the formula:

21. The method according to claim 20 wherein the anti-cancer agent is selected from the group consisting of the following compounds or a pharmaceutically acceptable salt therof:

(i) 1- (4-amino-2-oxo-lH-pyrimidin-l-yl) -2-desoxy-2 ' , 2 ' - difluororibose,

(ii) 1- (4-amino-2-oxo-lH-pyrimidin-l-yl) -2-desoxy- 2 ' , 2 ' -difluoroxylose,

(iii) 1- (2 , 4-dioxo-lH, 3H-pyrimidin-l-yl) -2-desoxy- 2 ', 2 ' -difluororibose, and

(iv) 1- (4-amino-5-methyl-2-oxo-lH-pyrimidin-l-yl) -2- desoxy-2 ' , 2 ' -difluororibose.

22. The method according to claim 19 wherein the 2 ' , 2 ' - difluornucleoside is gemcitabine HCl , namely 2 ' -deoxy-2 ' , 2 ' - difluorocytidine monohydrochloride (β-isomer)or l-(4-amino- 2-oxo-lH-pyrimidin-l-yl) -2-desoxy-2 ' , 2 ' -difluororibose.

23. The method of claim 18 or 19 or 20 or 21 or 22 wherein the leukotriene (LTB4) antagonist is represented by the formula (I)

(1) wherein :

X is selected from the group consisting of,

(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; and

(ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i) ;

Yi is a bond or divalent linking group containing 1 to 9 atoms ;

Y2 and Y3 are divalent linking groups independently selected from -CH2-, -0-, or -S-; Z is an Acidic Group;

Rl is C -Cio alkyl, aryl, C3-C8 cycloalkyl, C2-C10 alkenyl, C2-C 0 alkynyl, C6-C20 aralkyl, Cg-C20 alkaryl, C1-C10 haloalkyl, C -C20 aryloxy, or CI-CIQ alkoxy;

R2 is hydrogen, halogen, CI-CIQ haloalkyl, CI-CIQ alkoxy, C1-C10 alkyl, C3-C8 cycloalkyl, Acidic Group, or - (CH2) 1-7- (Acidic Group);

R3 is hydrogen, halogen, CI-CIQ alkyl, aryl, CI-CIQ haloalkyl, C -CIQ alkoxy, C5-C20 aryloxy, or C3-C8 cycloalkyl ;

R4 is C1-C4 alkyl, C3-C4 cycloalkyl, - (CH2) 1-7- (C3-C4 cycloalkyl), C2-C4 alkenyl, C2-C4 alkynyl, benzyl, or aryl; and

n is 0, 1, 2, 3, 4, 5, or 6;

or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof .

24. The method according to claim 23 wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following formulae:

where RIO is a radical selected from hydrogen or

C1-C4 alkyl; and Rll is a radical selected from hydrogen, halo, C1-C10 alkyl, CI-CIQ haloalkyl, CI-CIQ alkoxy, aryl, or C5-C20 aryloxy.

25. The method according to claim 24 wherein the Rl, R2, R3 and R4 groups for substitution in formula (I) are selected from the following variables coded R01 thru R16

and;

the Yl, Y2 , and Y3 groups for substitution in formula (I) are selected from the following variables coded YOl thru Y27:

and;

the X and Z groups and the n variable for substitution in formula (I) are selected from the following variables coded XZnOl thru XZn24:

26. A method according to claim 23 wherein the leukotriene B4 antagonist is described by formula (II)

wherein;

X2 is a heterocyclic radical selected from,

or

R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and

R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.

Z2 is the Acidic Group selected from carboxyl, tetrazolyl, or N-sulfonamidyl ;

or a salt, solvate or prodrug thereof.

27. The method according to claim 23, wherein the leukotriene antagonist is a compound selected from the following:

10

or an acid, salt, solvate or prodrug derivative thereof

28. The method according to claim 23 wherein the leukotriene antagonist is a compound selected from the following:

or an acid, salt, solvate or prodrug derivative thereof.

29. The method of claim 18 or 19 or 20 or 21 or 22 wherein the leukotriene (LTB4) antagonist is represented by a compound of the structure (Formula A) :

Formula A

or a pharmaceutically acceptable base addition salt thereof, wherein:

Rl' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2-substituted phenyl; each R2 ' and R3 • are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1-C4 alkoxy, (C1-C4 alkyl) - (0) g S-, trifluoromethyl, or di- (C1-C3 alkyl) amino;

X' is -0-, -S-, -C(=0), or -CH2-;

Y' is -0- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C≡C-; Z' is a straight or branched chain Ci-Cio alkylidenyl;

A' is a bond, -0-, -S-, -CH=CH-, or -CR_aRj^_ _• where R_a and Rjh are each independently hydrogen, C1-C5 alkyl, or R7- substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring;

wherein : each R6 is independently -COOH, 5-tetrazolyl, -

CON(Rg)2' or -CONHS0 Rιo; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-R6, -T-G-R6, (C1-C4 alkyl) -T- (C1-C4 alkylidenyl) -0-, or hydroxy;

R8 is hydrogen or halo; each R9 is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;

RlO is C1-C4 alkyl or phenyl; RU is R2, -W-R6, or -T-G-R6; each W is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0-, -NH-, -NHCO-, -C(=0)-, or (0)g S-;

K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2; p is 0 or 1; and t is 0 or 1; provided when X is -0- or -S-, Y is not -O- ; provided when A is -0- or -S-, R is not Rδ; and provided W is not a bond when p is 0.

30. The method of claim 29 wherein R4 ' is selected from the following formulae:

31. The method of claim 30 wherein R4 ' is

32. The method of claim 29 wherein said compound is selected from the group (A) to (KKKK) consisting of:

A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) heptane;

B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- ( 3-fluorophenyl ) -5-hydroxyphenoxy) heptane;

C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- dimethylaminocarbonylbutyloxy) phenyl ) propionic acid;

D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

E) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- carboxybutyloxy) phenyl ) propionic acid;

F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid;

G) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl ) butyloxy) phenyl ) propionic acid;

H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) - (1- butenyl ) ) phenyl ) propionate; I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl ) ) phenyl ) propionic acid;

J) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) phenyl ) propionic acid;

K) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) buty1 ) -6- methoxyphenyl) propionic acid;

L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionate;

M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid;

N) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- butyloxy) phenyl) propionic acid; 0) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- ( 4- methylthiobutyloxy) phenyl) propionic acid;

P) 3- (2- (3- (2, 4-Di- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutoxy) phenyl) propionic acid;

Q) 6-Methyl-6- (lH-tetrazol-5-yl) -11- (2-ethyl-4- ( 4-fluorophenyl ) -5 -hydroxyphenoxy) undecane ;

R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; S) N-Methanesulfonyl-3-(2-(3-(2-ethyl-4-(4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ;

T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; U) 3- (2- (3- (2-Butyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; V) Ethyl 3-(2-(4-(2-ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) butyloxy) phenyl ) propionate;

W) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionic acid;

X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- (4- (methoxycarbonyl ) phenoxy) phenyl ) propionate;

Y) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxyphenoxy) phenyl ) propionic acid; Z) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -4- ( 4- carboxyphenoxy) phenyl ) propionic acid;

AA) 3 , 3-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane;

CC) 2-Methyl-2- (lH-tetrazol-5-yl) -3-hydroxy-3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ;

DD) 3- (2- (3- (2-Bromo-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;

EE) 3- (2- (3- (2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; FF) Methyl 3- (2-hydroxy-3- (4- methoxycarbonylbutyl) -6- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionate; GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- (4- carboxybutyl ) dihydrocoumarin;

HH) 2-Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxy] phenol sodium salt;

II) 2- (4-Methylphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl)heptyloxy]phenol disodium salt;

JJ) 2- (3-Methylphenyl) -4-ethyl-5- [ 6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;

KK) 2- (2-Methylphenyl) -4-ethyl-5- [ 6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

LL) 2- (4-Methoxyphenyl) -4-ethyl-5- [6-methyl-6-

( 2H-tetrazol-5-yl ) heptyloxy] phenol sodium salt; MM) 2- (3-Methoxyphenyl)-4-ethyl-5- [6-methyl-6-

(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;

NN) 2- ( 4-Trifluoromethylphenyl) - -ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl)heptyloxy]phenol disodium salt;

00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;

PP) 3- (5- (6- (4-Phenyl-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3,4 -tetrahydronaphthalen-1 (2H) - one) propanoic acid;

QQ) 3- (5- (6-(4-(4-Fluorophenyl)-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2, 3, 4-tetrahydronaphthalen-1 (2H) - one) propanoic acid; RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl-2 , 3- dihydroinden-1 (2H) -one) propanoic acid; SS) 3,3-Dimethyl-5-(3-(2-carboxyethyl)-4-(3-(4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl) -5-oxopentanoic acid; TT) 7- [3- [ (5-Ethyl-2-hydroxy [1, 1 ' -biphenyl] -4- yl) oxy] propoxy] -3 , 4-dihydro-8-propyl-2H-l- benzopyran-2-carboxylic acid;

UU) 8-Propyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-carboxylic acid;

W) 2-[3-[3-[ ( 5-Ethyl-2-hydroxy [1,1" -biphenyl] - 4-yl) oxy] propoxy] -2-propylphenoxy] propanoic acid;

WW) 2- (4-Chlorophenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt;

XX) 2- (3, 5-Dichlorophenyl)-4-ethyl-5-[6-methyl- 6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt; YY) 3-[2-[3-[ (5-Ethyl-2-hydroxy[l,l' -biphenyl] -

4-yl) oxy] propoxy] -1-dibenzofuran] propanoic acid disodium salt;

ZZ) 7-Carboxy-9-oxo-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;

AAA) 2- [2-Propyl-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy]benzoic acid sodium salt hemihydrate;

BBB) 3-[3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl] -4- propanoic acid disodium salt monohydrate;

CCC) 5-Ethyl-4- [3- [2-propyl-3- [2- (2H-tetrazol-5- yl ) phenoxy] phenoxy] propoxy] [1,1' -biphenyl] - 2-ol disodium salt sesquihydrate; DDD) 3-[4-[3-[ 3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ] propanoic acid sodium salt hemihydrate;

EEE) 2-Fluoro-6- [2-propyl-3- [3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt;

FFF) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt;

GGG) 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4- fluorophenyl) -5 -hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate;

HHH) 3- [4- [9-0x0-3- [3- [2-ethyl-4- (4- fluorophenyl) -5 -hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid;

III) 3- [2- [1- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid; JJJ) 2-Fluoro-6-[2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate; KKK) 4-Fluoro-2-[2-propyl-3-[3-[2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; LLL) 2- [2-Propyl-3- [5- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] pentoxy] phenoxy] benzoic acid;

MMM) 2- [2-Propyl-3- [4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] butoxy] phenoxy] benzoic acid sesquihydrate; NNN) 2- [2- (2-Methylpropyl) -3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;

OOO) 2- [2-Butyl-3- [3- [2 -ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid hydrate; PPP) 2- [2- (Phenylmethyl )-3- [3- [2-ethyl-5-hydroxy-

4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; QQQ) 2-[2-Propyl-3-[3-[2-ethyl-5-hydroxy-4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] phenylacetic acid;

RRR) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid;

SSS) 2- [ [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4.- fluorophenyl ) phenoxy] propoxy] phenyl ] methyl ] b enzoic acid;

TTT) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid;

UUU) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenylsulfinyl]benzoi c acid;

VW) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfonyl ] benzoic acid hydrate;

WWW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- ( 4-fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenyl] -4-pentynoic acid disodium salt 0.4 hydrate;

XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- ( 4-fluorophenyl ) -5-hydroxyphenoxy) hexane ; YYY) 1- (4- (Carboxymethoxy) phenyl) -1- (IH-tetrazol- 5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane;

ZZZ) 1- (4- (Dimethylaminocarbonylmethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane; AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy)propoxy) phenyl) -E-propenoic acid;

BBBB) 3- (2- (3- (2-Ethyl-4- (4- luorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) -2-methyl-E- propenoic acid;

CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl ) -1H- tetrazole;

DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- (4- carboxybutyloxy) phenyl ) propionic acid;

EEEE) 5- [3- [4- (4-Fluorophenyl ) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-one; FFFF) 3- (3-{3-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;

GGGG) 3- (3- {3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4-propylph enyl) propanoic acid sodium salt;

HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -3- propylphenyl ) propanoic acid;

IIII) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl ) propanoic acid;

JJJJ) 3-{3-[3- (2-Ethyl-5- hydroxyphenyloxy) propoxy] -2- propylphenyl}propanoic acid disodium salt; and

KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] benzoyl] benzoic acid disodium salt hemihydrate.

33. The method of claim 18 or 19 or 20 or 21 or 22 wherein the leukotriene (LTB4) antagonist is a compound of the structure (Formula B) :

Formula B

namely, 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy]propoxy]phenoxy] benzoic acid, and the pharmaceutically acceptable salts thereof.

34. The method of claim 18 wherein the anti-cancer agent is a therapeutically effective amount of a 2',2'- difluoronucleoside anti-cancer agent according to the formula:

wherein :

Rl is hydrogen or

R2 is a base defined by one of the formulae

X is N or C-R⁴

R3 is hydrogen, C1-C4 alkyl or

R⁴ is hydrogen, C1-C4 alkyl, amino, bromo, fluoro, chloro or iodo; each R5 independently is hydrogen or C1-C4 alkyl; and the pharmaceutically-acceptable salts thereof.

35. A method of treating cancer in a mammalian patient by administering to said patient a therapeutically effective amount of a leukotriene (LTB4) antagonist and a therapeutically effective amount of 2 ', 2 ' -difluoronucleoside anti-cancer agent; wherein the anti-cancer agent is gemcitabine hydrochloride and the leukotriene (LTB4) antagonist is a compound of the structure (Formula B)

or pharmaceutically acceptable salts thereof.

36. The method of claim 18 or 19 or 35 wherein the weight ratio of LTB4 antagonist to anti-cancer agent 1:100 to 100 to 1.

37. The method of claim 18 or 19 or 35 wherein the combined dose weight of LTB4 antagonist and anti-cancer agentin from 0.5 to about 300 mg/kg per day.