WO2001032839A3 - Medium for preparing dedifferentiated cells - Google Patents

Medium for preparing dedifferentiated cells Download PDF

Info

Publication number
WO2001032839A3
WO2001032839A3 PCT/CA2000/001284 CA0001284W WO0132839A3 WO 2001032839 A3 WO2001032839 A3 WO 2001032839A3 CA 0001284 W CA0001284 W CA 0001284W WO 0132839 A3 WO0132839 A3 WO 0132839A3
Authority
WO
WIPO (PCT)
Prior art keywords
medium
dedifferentiated cells
preparing
preparing dedifferentiated
langerhans
Prior art date
Application number
PCT/CA2000/001284
Other languages
French (fr)
Other versions
WO2001032839A2 (en
Inventor
Lawrence Rosenberg
Original Assignee
Univ Mcgill
Lawrence Rosenberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Mcgill, Lawrence Rosenberg filed Critical Univ Mcgill
Priority to EP00972511A priority Critical patent/EP1224263A2/en
Priority to CA002388208A priority patent/CA2388208A1/en
Priority to IL14929000A priority patent/IL149290A0/en
Priority to JP2001535521A priority patent/JP2003513624A/en
Priority to AU11234/01A priority patent/AU1123401A/en
Priority to MXPA02004268A priority patent/MXPA02004268A/en
Publication of WO2001032839A2 publication Critical patent/WO2001032839A2/en
Publication of WO2001032839A3 publication Critical patent/WO2001032839A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/22—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from pancreatic cells

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a medium for preparing dedifferentiated cells derived from post-natal islets of Langerhans. The medium comprises in a physiologically acceptable culture medium an effective amount of a solid matrix environment for a three-dimensional culture, a soluble matrix protein, and a first and a second factor for developing, maintaining and expanding the dedifferentiated cells. Such a medium may be used in an in vitro method for islet cell expansion.
PCT/CA2000/001284 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells WO2001032839A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00972511A EP1224263A2 (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells
CA002388208A CA2388208A1 (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells
IL14929000A IL149290A0 (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells
JP2001535521A JP2003513624A (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells
AU11234/01A AU1123401A (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells
MXPA02004268A MXPA02004268A (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16213799P 1999-10-29 1999-10-29
US60/162,137 1999-10-29

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10111485 A-371-Of-International 2002-08-07
US10/421,363 Continuation-In-Part US20040018623A1 (en) 1999-10-29 2003-04-23 Medium for preparing dedifferentiated cells
US10/426,255 Continuation-In-Part US20040033599A1 (en) 1999-10-29 2003-04-29 Medium for preparing dedifferentiated cells

Publications (2)

Publication Number Publication Date
WO2001032839A2 WO2001032839A2 (en) 2001-05-10
WO2001032839A3 true WO2001032839A3 (en) 2001-11-01

Family

ID=22584317

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/001284 WO2001032839A2 (en) 1999-10-29 2000-10-27 Medium for preparing dedifferentiated cells

Country Status (7)

Country Link
EP (1) EP1224263A2 (en)
JP (1) JP2003513624A (en)
AU (1) AU1123401A (en)
CA (1) CA2388208A1 (en)
IL (1) IL149290A0 (en)
MX (1) MXPA02004268A (en)
WO (1) WO2001032839A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003102134A2 (en) * 2002-05-28 2003-12-11 Becton, Dickinson And Company Pancreatic acinar cells into insulin producing cells
EP1636348B1 (en) * 2003-06-20 2009-07-29 Vrije Universiteit Brussel VUB Method of generating islet beta-cells from exocrine pancreatic cells
EP1814980B1 (en) * 2004-11-22 2015-03-25 Ramot at Tel-Aviv University Ltd. Populations of expanded and re-differentiated adult islet beta cells capable of producing insulin and methods of generating same
CA2900561A1 (en) * 2013-02-25 2014-08-28 Genentech, Inc. Liquid culturing of epithelial stem cells
WO2015058139A1 (en) * 2013-10-18 2015-04-23 Symbiocelltech, Llc Stem cell / islet cell hybrids, generation thereof, and methods for the treatment and cure of insulin-dependent diabetes mellitus

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016715A1 (en) * 1993-01-27 1994-08-04 Hemosol, Inc. Selective cell proliferation
WO1997015310A1 (en) * 1995-10-25 1997-05-01 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of langerhans and in vivo uses thereof
WO1997016536A1 (en) * 1995-10-30 1997-05-09 Vivorx, Inc. Method for ex vivo proliferation and differentiation of adult pancreatic islet cells, media useful therefor and uses thereof
WO2000046351A2 (en) * 1999-02-04 2000-08-10 Mcgill University Platform for the differentiation of cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016715A1 (en) * 1993-01-27 1994-08-04 Hemosol, Inc. Selective cell proliferation
WO1997015310A1 (en) * 1995-10-25 1997-05-01 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of langerhans and in vivo uses thereof
WO1997016536A1 (en) * 1995-10-30 1997-05-09 Vivorx, Inc. Method for ex vivo proliferation and differentiation of adult pancreatic islet cells, media useful therefor and uses thereof
WO2000046351A2 (en) * 1999-02-04 2000-08-10 Mcgill University Platform for the differentiation of cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ROSENBERG L: "IN VIVO CELL TRANSFORMATION: NEOGENESIS OF BETA CELLS FROM PANCREATIC DUCTAL CELLS", CELL TRANSPLANTATION,ELSEVIER SCIENCE,US, vol. 4, no. 4, 1995, pages 371 - 383, XP000939136, ISSN: 0963-6897 *
YUAN S ET AL: "TRANSDIFFERENTIATION OF HUMAN ISLETS TO PANCREATIC DUCTAL CELLS IN COLLAGEN MATRIX CULTURE", DIFFERENTIATION,SPRINGER VERLAG,DE, vol. 61, no. 1, 1996, pages 67 - 75, XP000939119, ISSN: 0301-4681 *

Also Published As

Publication number Publication date
CA2388208A1 (en) 2001-05-10
MXPA02004268A (en) 2003-01-28
WO2001032839A2 (en) 2001-05-10
EP1224263A2 (en) 2002-07-24
IL149290A0 (en) 2002-11-10
AU1123401A (en) 2001-05-14
JP2003513624A (en) 2003-04-15

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