AU2002213143A1 - Non-disruptive three-dimensional culture and harvest system for anchorage-dependent cells - Google Patents

Non-disruptive three-dimensional culture and harvest system for anchorage-dependent cells

Info

Publication number
AU2002213143A1
AU2002213143A1 AU2002213143A AU1314302A AU2002213143A1 AU 2002213143 A1 AU2002213143 A1 AU 2002213143A1 AU 2002213143 A AU2002213143 A AU 2002213143A AU 1314302 A AU1314302 A AU 1314302A AU 2002213143 A1 AU2002213143 A1 AU 2002213143A1
Authority
AU
Australia
Prior art keywords
disruptive
anchorage
dimensional culture
dependent cells
harvest system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002213143A
Inventor
Ser-Mien Chia
Kam W Leong
Harry Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National University of Singapore
Institute of Materials Research and Engineering
Original Assignee
National University of Singapore
Institute of Materials Research and Engineering
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University of Singapore, Institute of Materials Research and Engineering filed Critical National University of Singapore
Publication of AU2002213143A1 publication Critical patent/AU2002213143A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/126—Immunoprotecting barriers, e.g. jackets, diffusion chambers
    • A61K2035/128—Immunoprotecting barriers, e.g. jackets, diffusion chambers capsules, e.g. microcapsules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan

Abstract

A multi-layered microcapsule has an inner extracellular matrix and an outer shell. The inner extracellular matrix includes a first inner layer of biopolymer and a second intermediate layer of polymer that provides partial immune-protection and holds the first layer in place. The outer shell can form an exoskeleton to provide mechanical stability. Each of the individual layers can be varied to optimize mechanical stability, cell function, and immuno-protection.
AU2002213143A 2000-10-12 2001-10-12 Non-disruptive three-dimensional culture and harvest system for anchorage-dependent cells Abandoned AU2002213143A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23925900P 2000-10-12 2000-10-12
US60239259 2000-10-12
PCT/US2001/031890 WO2002031135A1 (en) 2000-10-12 2001-10-12 Non-disruptive three-dimensional culture and harvest system for anchorage-dependent cells

Publications (1)

Publication Number Publication Date
AU2002213143A1 true AU2002213143A1 (en) 2002-04-22

Family

ID=22901354

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002213143A Abandoned AU2002213143A1 (en) 2000-10-12 2001-10-12 Non-disruptive three-dimensional culture and harvest system for anchorage-dependent cells

Country Status (6)

Country Link
US (4) US6916640B2 (en)
EP (1) EP1326968B1 (en)
AT (1) ATE360063T1 (en)
AU (1) AU2002213143A1 (en)
DE (1) DE60127983T2 (en)
WO (1) WO2002031135A1 (en)

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Also Published As

Publication number Publication date
EP1326968A1 (en) 2003-07-16
ATE360063T1 (en) 2007-05-15
DE60127983D1 (en) 2007-05-31
US20090286278A1 (en) 2009-11-19
US20040023370A1 (en) 2004-02-05
US6905875B2 (en) 2005-06-14
WO2002031135A1 (en) 2002-04-18
DE60127983T2 (en) 2008-01-17
EP1326968A4 (en) 2004-10-06
US20050220891A1 (en) 2005-10-06
US6916640B2 (en) 2005-07-12
US7943353B2 (en) 2011-05-17
US20020094569A1 (en) 2002-07-18
EP1326968B1 (en) 2007-04-18

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