WO2001032838A3 - Methods for improving viral vector titers and reducing cell death in cell cultures - Google Patents

Methods for improving viral vector titers and reducing cell death in cell cultures Download PDF

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Publication number
WO2001032838A3
WO2001032838A3 PCT/US2000/029549 US0029549W WO0132838A3 WO 2001032838 A3 WO2001032838 A3 WO 2001032838A3 US 0029549 W US0029549 W US 0029549W WO 0132838 A3 WO0132838 A3 WO 0132838A3
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WO
WIPO (PCT)
Prior art keywords
viral vector
methods
vector titers
cell death
reducing
Prior art date
Application number
PCT/US2000/029549
Other languages
French (fr)
Other versions
WO2001032838A2 (en
Inventor
Manish Singh
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Priority to JP2001535520A priority Critical patent/JP2003513623A/en
Priority to AU13465/01A priority patent/AU1346501A/en
Priority to EP00975409A priority patent/EP1224260A2/en
Priority to CA002389057A priority patent/CA2389057A1/en
Publication of WO2001032838A2 publication Critical patent/WO2001032838A2/en
Publication of WO2001032838A3 publication Critical patent/WO2001032838A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141—Use of virus, viral particle or viral elements as a vector
    • C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed are compositions and methods for the increasing the production of a recombinant viral vector in a cell culture, comprising culturing a recombinant viral vector producing cell line in a basal media which further comprises (a) greater than 1.5 g/L amino acids (or, a selected amino acids in a quantity and amount greater than present in a basal media such as DMEM), and (b) between 0.5% and 4% serum, such that the recombinant viral vectors are produced.
PCT/US2000/029549 1999-10-29 2000-10-27 Methods for improving viral vector titers and reducing cell death in cell cultures WO2001032838A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2001535520A JP2003513623A (en) 1999-10-29 2000-10-27 Methods for improving viral vector titers and reducing cell death in cell culture
AU13465/01A AU1346501A (en) 1999-10-29 2000-10-27 Methods for improving viral vector titers and reducing cell death in cell cultures
EP00975409A EP1224260A2 (en) 1999-10-29 2000-10-27 Methods for improving viral vector titers and reducing cell death in cell cultures
CA002389057A CA2389057A1 (en) 1999-10-29 2000-10-27 Methods for improving viral vector titers and reducing cell death in cell cultures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16234699P 1999-10-29 1999-10-29
US60/162,346 1999-10-29

Publications (2)

Publication Number Publication Date
WO2001032838A2 WO2001032838A2 (en) 2001-05-10
WO2001032838A3 true WO2001032838A3 (en) 2002-01-17

Family

ID=22585237

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/029549 WO2001032838A2 (en) 1999-10-29 2000-10-27 Methods for improving viral vector titers and reducing cell death in cell cultures

Country Status (5)

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EP (1) EP1224260A2 (en)
JP (1) JP2003513623A (en)
AU (1) AU1346501A (en)
CA (1) CA2389057A1 (en)
WO (1) WO2001032838A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003430A1 (en) * 1988-09-23 1990-04-05 Cetus Corporation Cell culture medium for enhanced cell growth, culture longevity and product expression
FR2744455A1 (en) * 1996-02-02 1997-08-08 Sanofi Sa NOVEL NUCLEIC ACID SEQUENCES IN THE FAMILY OF TUMOR SUPPRESSOR FACTORS, CORRESPONDING EXPRESSION PRODUCTS, AND APPLICATIONS THEREOF
US5792479A (en) * 1994-08-16 1998-08-11 Discovision Associates Technique for acceleration of apoptotic cell death

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003430A1 (en) * 1988-09-23 1990-04-05 Cetus Corporation Cell culture medium for enhanced cell growth, culture longevity and product expression
US5792479A (en) * 1994-08-16 1998-08-11 Discovision Associates Technique for acceleration of apoptotic cell death
FR2744455A1 (en) * 1996-02-02 1997-08-08 Sanofi Sa NOVEL NUCLEIC ACID SEQUENCES IN THE FAMILY OF TUMOR SUPPRESSOR FACTORS, CORRESPONDING EXPRESSION PRODUCTS, AND APPLICATIONS THEREOF

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DURBIN A. P. ET AL.: "The immunogenicity and efficacy of intranasally or parenterally administered replication-deficient vaccinia-parainfluenza virus type 3 recombinants in rhesus monkeys", VACCINE, vol. 16, no. 13, 1 August 1998 (1998-08-01), pages 1324 - 1330, XP004124600, ISSN: 0264-410X *
TAKESHI KURITA ET AL.: "APOPTOTIC CELL DEATH INDUCED BY SERUM AND ITS PREVENTION BY THIOLS", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 161, no. 1, 1 October 1994 (1994-10-01), pages 63 - 70, XP000560335, ISSN: 0021-9541 *

Also Published As

Publication number Publication date
CA2389057A1 (en) 2001-05-10
EP1224260A2 (en) 2002-07-24
AU1346501A (en) 2001-05-14
JP2003513623A (en) 2003-04-15
WO2001032838A2 (en) 2001-05-10

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