WO2001031989A3 - High throughput screening (hts) assays for protein variants with reduced antibody binding capacity - Google Patents

High throughput screening (hts) assays for protein variants with reduced antibody binding capacity Download PDF

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Publication number
WO2001031989A3
WO2001031989A3 PCT/DK2000/000682 DK0000682W WO0131989A3 WO 2001031989 A3 WO2001031989 A3 WO 2001031989A3 DK 0000682 W DK0000682 W DK 0000682W WO 0131989 A3 WO0131989 A3 WO 0131989A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibody binding
binding capacity
protein
variants
assays
Prior art date
Application number
PCT/DK2000/000682
Other languages
French (fr)
Other versions
WO2001031989A2 (en
Inventor
Erwin Ludo Roggen
Steffen Ernst
Henrik Pedersen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Priority to CA002394377A priority Critical patent/CA2394377A1/en
Priority to AU18522/01A priority patent/AU779195B2/en
Priority to EP00981185A priority patent/EP1240513A2/en
Publication of WO2001031989A2 publication Critical patent/WO2001031989A2/en
Publication of WO2001031989A3 publication Critical patent/WO2001031989A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Abstract

The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. The present invention relates more specifically to a method for screening a library of protein variants for functional variants with reduced antibody binding capacity, comprising the steps of: (i) generating a diversified library of protein variants starting from a relevant protein backbone, (ii) transforming the library into suitable host cells, (iii) culturing host cells, (iv) sampling each cell culture, (v) analysing a sample by determining the antibody binding capacity of the variant protein, (vi) analysing a sample by determining the functionality of the variant protein.
PCT/DK2000/000682 1999-12-09 2000-12-08 High throughput screening (hts) assays for protein variants with reduced antibody binding capacity WO2001031989A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002394377A CA2394377A1 (en) 1999-12-09 2000-12-08 High throughput screening (hts) assays for protein variants with reducedantibody binding capacity
AU18522/01A AU779195B2 (en) 1999-12-09 2000-12-08 High throughput screening (HTS) assays for protein variants with reduced antibody binding capacity
EP00981185A EP1240513A2 (en) 1999-12-09 2000-12-08 High throughput screening (hts) assays for protein variants with reduced antibody binding capacity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199901765 1999-12-09
DKPA199901765 1999-12-09

Publications (2)

Publication Number Publication Date
WO2001031989A2 WO2001031989A2 (en) 2001-05-10
WO2001031989A3 true WO2001031989A3 (en) 2001-11-15

Family

ID=8107893

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000682 WO2001031989A2 (en) 1999-12-09 2000-12-08 High throughput screening (hts) assays for protein variants with reduced antibody binding capacity

Country Status (4)

Country Link
EP (1) EP1240513A2 (en)
AU (1) AU779195B2 (en)
CA (1) CA2394377A1 (en)
WO (1) WO2001031989A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60335640D1 (en) 2002-10-01 2011-02-17 Novozymes As POLYPEPTIDES OF THE GH-61 FAMILY
WO2005066339A2 (en) 2004-01-06 2005-07-21 Novozymes A/S Polypeptides of alicyclobacillus sp.
US8119387B2 (en) 2005-08-16 2012-02-21 Novozymes A/S Polypeptides of strain Bacillus sp. P203

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038978A1 (en) * 1998-01-31 1999-08-05 University Of Arkansas Methods and reagents for decreasing allergic reactions
WO1999053049A1 (en) * 1998-04-15 1999-10-21 Abgenix, Inc. Epitope-driven human antibody production and gene expression profiling
WO2000026230A1 (en) * 1998-10-30 2000-05-11 Novozymes A/S Low allergenic protein variants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038978A1 (en) * 1998-01-31 1999-08-05 University Of Arkansas Methods and reagents for decreasing allergic reactions
WO1999053049A1 (en) * 1998-04-15 1999-10-21 Abgenix, Inc. Epitope-driven human antibody production and gene expression profiling
WO2000026230A1 (en) * 1998-10-30 2000-05-11 Novozymes A/S Low allergenic protein variants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SLOOTSTRA J W ET AL: "Screening of a Small Set of Random Peptides: A New Strategy to Identify Synthetic Peptides that Mimic Epitopes", JOURNAL OF MOLECULAR RECOGNITION, vol. 10, - 1997, pages 217 - 224, XP002901694 *
SLOOTSTRA J W ET AL: "Structual Aspects of Antibody-antigen Interaction Revealed through Small Random Peptide Libraries", MOLECULAR DIVERSITY, vol. 1, - 1995, pages 87 - 96, XP002901695 *

Also Published As

Publication number Publication date
AU779195B2 (en) 2005-01-13
AU1852201A (en) 2001-05-14
EP1240513A2 (en) 2002-09-18
CA2394377A1 (en) 2001-05-10
WO2001031989A2 (en) 2001-05-10

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