WO2001031033A3 - Protease from lucila sericata and its use in treatment of wounds - Google Patents

Protease from lucila sericata and its use in treatment of wounds Download PDF

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Publication number
WO2001031033A3
WO2001031033A3 PCT/GB2000/004034 GB0004034W WO0131033A3 WO 2001031033 A3 WO2001031033 A3 WO 2001031033A3 GB 0004034 W GB0004034 W GB 0004034W WO 0131033 A3 WO0131033 A3 WO 0131033A3
Authority
WO
WIPO (PCT)
Prior art keywords
wounds
amc
treatment
proteolytic activity
activity against
Prior art date
Application number
PCT/GB2000/004034
Other languages
French (fr)
Other versions
WO2001031033A2 (en
Inventor
David Idris Pritchard
Original Assignee
Univ Nottingham
David Idris Pritchard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nottingham, David Idris Pritchard filed Critical Univ Nottingham
Priority to DE60044584T priority Critical patent/DE60044584D1/en
Priority to AU79345/00A priority patent/AU7934500A/en
Priority to EP00969689A priority patent/EP1222285B1/en
Priority to AT00969689T priority patent/ATE471985T1/en
Priority to US10/111,252 priority patent/US7144721B1/en
Publication of WO2001031033A2 publication Critical patent/WO2001031033A2/en
Publication of WO2001031033A3 publication Critical patent/WO2001031033A3/en
Priority to US11/515,156 priority patent/US20070066538A1/en
Priority to US13/083,495 priority patent/US20110280858A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43577—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

An isolated protein, for use in the treatment of wounds, is characterised in that it is secreted by the organism Lucilia sericata and it exhibits optimum proteolytic activity against FITC-casein at a pH of 8.0 to 8.5. The protein exhibits proteolytic activity against Tosyl-Gly-Pro-Arg-AMC but not against Suc-Ala-Ala-Phe-AMC, and its proteolytic activity against FITC-casein and Tosyl-Gly-Pro-Arg-AMC is inhibited by the serine proteinase inhibitors PMSF and APMSF; Furthermore, the protein is bound by immobilised aminobenzamidine.
PCT/GB2000/004034 1999-10-22 2000-10-20 Protease from lucila sericata and its use in treatment of wounds WO2001031033A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE60044584T DE60044584D1 (en) 1999-10-22 2000-10-20 PROTEASE FROM LUCILIA SERICATA AND ITS USE IN WOUND HEALING
AU79345/00A AU7934500A (en) 1999-10-22 2000-10-20 The treatment of wounds
EP00969689A EP1222285B1 (en) 1999-10-22 2000-10-20 Protease from lucila sericata and its use in treatment of wounds
AT00969689T ATE471985T1 (en) 1999-10-22 2000-10-20 PROTEASE FROM LUCILIA SERICATA AND THEIR USE FOR WOUND HEALING
US10/111,252 US7144721B1 (en) 1999-10-22 2000-10-20 Treatment of wounds
US11/515,156 US20070066538A1 (en) 1999-10-22 2006-08-31 Treatment of wounds
US13/083,495 US20110280858A1 (en) 1999-10-22 2011-04-08 Treatment of wounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9925005.2A GB9925005D0 (en) 1999-10-22 1999-10-22 The treatment of wounds
GB9925005.2 1999-10-22

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/823,209 Continuation-In-Part US20080108551A1 (en) 1999-10-22 2007-06-27 Treatment of wounds

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/515,156 Division US20070066538A1 (en) 1999-10-22 2006-08-31 Treatment of wounds

Publications (2)

Publication Number Publication Date
WO2001031033A2 WO2001031033A2 (en) 2001-05-03
WO2001031033A3 true WO2001031033A3 (en) 2001-09-20

Family

ID=10863184

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004034 WO2001031033A2 (en) 1999-10-22 2000-10-20 Protease from lucila sericata and its use in treatment of wounds

Country Status (7)

Country Link
US (2) US7144721B1 (en)
EP (1) EP1222285B1 (en)
AT (1) ATE471985T1 (en)
AU (1) AU7934500A (en)
DE (1) DE60044584D1 (en)
GB (1) GB9925005D0 (en)
WO (1) WO2001031033A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19901134C2 (en) * 1999-01-14 2002-11-21 Wilhelm Fleischmann dressing material
US20080108551A1 (en) * 2006-07-06 2008-05-08 The Secretary Of State For Defence Treatment of wounds
DE10138303A1 (en) * 2001-08-10 2003-03-06 Aventis Pharma Gmbh New extracts of fly larvae, useful as medicaments for promoting wound healing, obtained by homogenizing fly larvae under cooling and removing non-dissolved components
GB0127618D0 (en) * 2001-11-17 2002-01-09 Univ Nottingham "Composition and method for treatment of wounds"
GB0205593D0 (en) * 2002-03-09 2002-04-24 Univ Nottingham Treatment of surfaces populated by bacteria
DE10342104A1 (en) * 2003-09-10 2005-06-09 Fleischmann, Wilhelm, Dr.med. Process for the preparation of a wound treatment composition and agent prepared by this process
DE102005061246A1 (en) * 2005-12-20 2007-06-28 Alpha-Biocare Gmbh Preparations containing low molecular weight substances from Diptera for the treatment of wounds
GB0607495D0 (en) * 2006-04-13 2006-05-24 Secr Defence Larval enzymes
GB0610697D0 (en) * 2006-05-31 2006-07-12 Secr Defence Larval enzyme
US20100008898A1 (en) * 2006-05-31 2010-01-14 David Idris Pritchard Chymotrypsin from lucilia sericata larvae and its use for the treatment of wounds
GB0700946D0 (en) * 2007-01-18 2007-02-28 Uws Ventures Ltd Antimicrobial composition and a method of controlling contamination and infection using said composition
EP2226382A1 (en) 2009-03-03 2010-09-08 B.R.A.I.N. Biotechnology Research and Information Network AG Protease for wound conditioning and skin care
US10513696B2 (en) 2016-05-24 2019-12-24 Hamzeh Alipour Lucilia sericata collagenase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987007907A1 (en) * 1986-06-17 1987-12-30 Celltech Limited Process for the production of a protein
WO1988003151A2 (en) * 1986-10-31 1988-05-05 Board Of Regents, The University Of Texas System Thrombin derived polypeptides; compositions and methods for use

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5688768A (en) 1991-02-19 1997-11-18 Cor Therapeutics, Inc. Recombinant thrombin receptor and related pharmaceuticals
US5892014A (en) 1996-10-30 1999-04-06 The Regents Of The University Of California DNA encoding a protease-activated receptor 3
KR20010041418A (en) 1998-02-27 2001-05-15 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Protease-activated receptor 4 and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987007907A1 (en) * 1986-06-17 1987-12-30 Celltech Limited Process for the production of a protein
WO1988003151A2 (en) * 1986-10-31 1988-05-05 Board Of Regents, The University Of Texas System Thrombin derived polypeptides; compositions and methods for use

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BOWLES V M ET AL: "CHARACTERIZATION OF PROTEOLYTIC AND COLLAGENOLYTIC ENZYMES FROM THE LARVAE OF LUCILIA-CUPRINA THE SHEEP BLOWFLY", AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, vol. 41, no. 2, 1988, pages 269 - 278, XP000978829, ISSN: 0004-9417 *
CONSTABLE S A: "A Comparison of Proteases Produced by Larvae of Lucilia cuprina (Wiedemann), L. sericata (Meigen), Calliphora augur (F.) and C. stygia (F.) (Diptera: Calliphoridae).", JOURNAL OF THE AUSTRALIAN ENTOMOLOGICAL SOCIETY, vol. 33, no. 3, 1994, pages 203 - 210, XP000980193, ISSN: 0004-9050 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, MUMCUOGLU K Y ET AL: "Maggot therapy for gangrene and osteomyelitis.", XP002158070, Database accession no. PREV199799567102 *
HAREFUAH, vol. 132, no. 5, 1997, pages 323 - 325, 384, ISSN: 0017-7768 *
SANDEMAN R M ET AL: "TRYPTIC AND CHYMOTRYPTIC PROTEASES RELEASED BY LARVAE OF THE BLOWFLY LUCILIA-CUPRINA", INTERNATIONAL JOURNAL FOR PARASITOLOGY, vol. 20, no. 8, 1990, pages 1019 - 1024, XP000978819, ISSN: 0020-7519 *
YOUNG ANNA R ET AL: "Characterization of ES products involved in wound initiation by Lucilia cuprina larvae.", INTERNATIONAL JOURNAL FOR PARASITOLOGY, vol. 26, no. 3, 1996, pages 245 - 252, XP000978826, ISSN: 0020-7519 *

Also Published As

Publication number Publication date
WO2001031033A2 (en) 2001-05-03
US20070066538A1 (en) 2007-03-22
AU7934500A (en) 2001-05-08
US7144721B1 (en) 2006-12-05
ATE471985T1 (en) 2010-07-15
EP1222285B1 (en) 2010-06-23
EP1222285A2 (en) 2002-07-17
DE60044584D1 (en) 2010-08-05
GB9925005D0 (en) 1999-12-22

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