WO2001030977A3 - 5r clavam producing microorganisms with deleted lat and cvm genes - Google Patents

5r clavam producing microorganisms with deleted lat and cvm genes Download PDF

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Publication number
WO2001030977A3
WO2001030977A3 PCT/EP2000/010283 EP0010283W WO0130977A3 WO 2001030977 A3 WO2001030977 A3 WO 2001030977A3 EP 0010283 W EP0010283 W EP 0010283W WO 0130977 A3 WO0130977 A3 WO 0130977A3
Authority
WO
WIPO (PCT)
Prior art keywords
clavam
cvm
lat
genes
deleted
Prior art date
Application number
PCT/EP2000/010283
Other languages
French (fr)
Other versions
WO2001030977A2 (en
Inventor
Barry Barton
Philip Andrew Greaves
Alison Michelle Griffin
Original Assignee
Smithkline Beecham Plc
Barry Barton
Philip Andrew Greaves
Alison Michelle Griffin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9925135.7A external-priority patent/GB9925135D0/en
Priority claimed from GBGB9925137.3A external-priority patent/GB9925137D0/en
Application filed by Smithkline Beecham Plc, Barry Barton, Philip Andrew Greaves, Alison Michelle Griffin filed Critical Smithkline Beecham Plc
Priority to JP2001533961A priority Critical patent/JP2003512832A/en
Priority to EP00975883A priority patent/EP1228218A2/en
Priority to AU13857/01A priority patent/AU1385701A/en
Publication of WO2001030977A2 publication Critical patent/WO2001030977A2/en
Publication of WO2001030977A3 publication Critical patent/WO2001030977A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Abstract

The invention relates to organisms producing increased levels of clavulanic acid and other 5R clavams, including those with strong β-lactamase inhibitory activity, in combination with low or undetectable levels of 5S clavams.
PCT/EP2000/010283 1999-10-22 2000-10-18 5r clavam producing microorganisms with deleted lat and cvm genes WO2001030977A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2001533961A JP2003512832A (en) 1999-10-22 2000-10-18 5R clabham-producing microorganism lacking LAT and CVM genes
EP00975883A EP1228218A2 (en) 1999-10-22 2000-10-18 5r clavam producing microorganisms with deleted lat and cvm genes
AU13857/01A AU1385701A (en) 1999-10-22 2000-10-18 Novel compounds

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9925135.7A GB9925135D0 (en) 1999-10-22 1999-10-22 Novel compounds
GB9925137.3 1999-10-22
GBGB9925137.3A GB9925137D0 (en) 1999-10-22 1999-10-22 Novel compounds
GB9925135.7 1999-10-22

Publications (2)

Publication Number Publication Date
WO2001030977A2 WO2001030977A2 (en) 2001-05-03
WO2001030977A3 true WO2001030977A3 (en) 2001-12-13

Family

ID=26316021

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010283 WO2001030977A2 (en) 1999-10-22 2000-10-18 5r clavam producing microorganisms with deleted lat and cvm genes

Country Status (4)

Country Link
EP (1) EP1228218A2 (en)
JP (1) JP2003512832A (en)
AU (1) AU1385701A (en)
WO (1) WO2001030977A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0308696D0 (en) * 2003-04-15 2003-05-21 Glaxo Group Ltd New process
GB0402799D0 (en) * 2004-02-09 2004-03-10 Sandoz Ag Pharmaceutical composition

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041886A1 (en) * 1995-06-09 1996-12-27 Smithkline Beecham Plc Process to increase the production of clavam antibiotics
WO1998033896A2 (en) * 1997-02-04 1998-08-06 Smithkline Beecham Plc Microorganisms with increased production of clavulanic acid

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041886A1 (en) * 1995-06-09 1996-12-27 Smithkline Beecham Plc Process to increase the production of clavam antibiotics
WO1998033896A2 (en) * 1997-02-04 1998-08-06 Smithkline Beecham Plc Microorganisms with increased production of clavulanic acid

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JENSEN S E ET AL.: "Biosynthesis and molecular genetics of clavulanic acid", ANTONIE VAN LEEUWENHOEK, vol. 75, no. 1-2, January 1999 (1999-01-01), pages 125 - 133, XP000937780 *
LIRAS P ET AL.: "Clavulanic acid, a beta-lactamase inhibitor: biosynthesis and molecular genetics", APPLIED MICROBIOLGY AND BIOTECHNOLOGY, vol. 54, no. 4, October 2000 (2000-10-01), pages 467 - 475, XP000998142 *
MOSHER R H ET AL.: "Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 43, no. 5, May 1999 (1999-05-01), pages 1215 - 1224, XP002165205 *
RODRIGUEZ-GARCIA A ET AL.: "The argG gene of Streptomyces clavuligerus has low homology to unstable argG from other actinomycetes: effect of amplification on clavulanic acid biosynthesis", GENE, vol. 167, no. 1, 1995, pages 9 - 15, XP004043010 *
VAN DEN BERG M A ET AL.: "Engineering aspects of beta-lactam biosynthesis", ANTONIE VAN LEEUWENHOEK, vol. 75, no. 1-2, January 1999 (1999-01-01), pages 155 - 161, XP000998156 *

Also Published As

Publication number Publication date
AU1385701A (en) 2001-05-08
WO2001030977A2 (en) 2001-05-03
EP1228218A2 (en) 2002-08-07
JP2003512832A (en) 2003-04-08

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