WO2001029253A3 - Simultaneous visualization of eu- and heterochromatin - Google Patents

Simultaneous visualization of eu- and heterochromatin Download PDF

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Publication number
WO2001029253A3
WO2001029253A3 PCT/EP2000/010269 EP0010269W WO0129253A3 WO 2001029253 A3 WO2001029253 A3 WO 2001029253A3 EP 0010269 W EP0010269 W EP 0010269W WO 0129253 A3 WO0129253 A3 WO 0129253A3
Authority
WO
WIPO (PCT)
Prior art keywords
heterochromatin
visualization
simultaneous visualization
probes
relates
Prior art date
Application number
PCT/EP2000/010269
Other languages
French (fr)
Other versions
WO2001029253A2 (en
Inventor
Michael Speicher
Roland Eils
Original Assignee
Michael Speicher
Roland Eils
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michael Speicher, Roland Eils filed Critical Michael Speicher
Priority to AU10263/01A priority Critical patent/AU1026301A/en
Publication of WO2001029253A2 publication Critical patent/WO2001029253A2/en
Publication of WO2001029253A3 publication Critical patent/WO2001029253A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for the visualization of eu- and heterochromatin comprising hybridizing to a target DNA a repeat-depleted probe and a probe specific for repetitive sequences. Further, the present invention relates to the use of said probes for the visualization of eu- and heterochromatin, as well as compositions, i.e., diagnostic compositions, and kits comprising said probes.
PCT/EP2000/010269 1999-10-18 2000-10-18 Simultaneous visualization of eu- and heterochromatin WO2001029253A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU10263/01A AU1026301A (en) 1999-10-18 2000-10-18 Simultaneous visualization of eu- and heterochromatin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99120617 1999-10-18
EP99120617.8 1999-10-18

Publications (2)

Publication Number Publication Date
WO2001029253A2 WO2001029253A2 (en) 2001-04-26
WO2001029253A3 true WO2001029253A3 (en) 2001-11-29

Family

ID=8239218

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010269 WO2001029253A2 (en) 1999-10-18 2000-10-18 Simultaneous visualization of eu- and heterochromatin

Country Status (2)

Country Link
AU (1) AU1026301A (en)
WO (1) WO2001029253A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5457673B2 (en) 2005-09-20 2014-04-02 ベリデックス・リミテッド・ライアビリティ・カンパニー Methods and compositions for making unique sequence DNA probes, labeling of DNA probes, and use of these probes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997022848A1 (en) * 1995-12-20 1997-06-26 Spectral Diagnostic Ltd. Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997022848A1 (en) * 1995-12-20 1997-06-26 Spectral Diagnostic Ltd. Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOLZER A ET AL: "COMPLETE SET OF REPEAT-DEPLETED, PCR-AMPLIFIABLE, HUMAN CHROMOSOME-SPECIFIC PAINTING PROBES", CYTOGENETICS AND CELL GENETICS,CH,BASEL, vol. 84, no. 3/04, 1999, pages 233 - 240, XP000907217, ISSN: 0301-0171 *
KATONA ROBERT ET AL: "Mouse euchromatin specific "genome-painting" with a LINE probe: A rapid method for identification and mapping of human chromosomes in mouse-human microcell hybrids by two-color FISH.", HEREDITAS (LUND), vol. 124, no. 2, 1996, pages 131 - 135, XP001007120, ISSN: 0018-0661 *
UHRIG SABINE ET AL: "Multiplex-FISH for pre- and postnatal diagnostic applications.", AMERICAN JOURNAL OF HUMAN GENETICS, vol. 65, no. 2, August 1999 (1999-08-01), pages 448 - 462, XP001007570, ISSN: 0002-9297 *

Also Published As

Publication number Publication date
AU1026301A (en) 2001-04-30
WO2001029253A2 (en) 2001-04-26

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