WO2001029252A3 - Procede permettant de produire de l'adn appauvri en unites de repetition - Google Patents

Procede permettant de produire de l'adn appauvri en unites de repetition Download PDF

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Publication number
WO2001029252A3
WO2001029252A3 PCT/EP2000/010268 EP0010268W WO0129252A3 WO 2001029252 A3 WO2001029252 A3 WO 2001029252A3 EP 0010268 W EP0010268 W EP 0010268W WO 0129252 A3 WO0129252 A3 WO 0129252A3
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WO
WIPO (PCT)
Prior art keywords
dna
repeat
generation
pcr
depleted
Prior art date
Application number
PCT/EP2000/010268
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English (en)
Other versions
WO2001029252A2 (fr
Inventor
Michael Speicher
Roland Eils
Original Assignee
Michael Speicher
Roland Eils
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michael Speicher, Roland Eils filed Critical Michael Speicher
Priority to AU77890/00A priority Critical patent/AU7789000A/en
Publication of WO2001029252A2 publication Critical patent/WO2001029252A2/fr
Publication of WO2001029252A3 publication Critical patent/WO2001029252A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant de produire de l'ADN appauvri en unités de répétition, qui comporte l'amplification d'ADN modèle répétitif par une première amplification en chaîne par polymérase (PCR) dont l'étape d'hybridation est peu restrictive ; et une deuxième PCR suivant la première PCR, l'étape d'hybridation de cette deuxième PCR étant fortement restrictive. L'ADN appauvri en unités de répétition obtenu peut être utilisé comme sonde ou cloné en vecteurs, plasmide, etc. De plus, l'invention concerne l'application du procédé dans la production de banques d'ADN, de groupements d'ADN ou de dispositifs de transfert d'ADN, et dans l'hybridation avec des banques d'ADN, des groupements d'ADN ou des dispositifs de transfert d'ADN.
PCT/EP2000/010268 1999-10-18 2000-10-18 Procede permettant de produire de l'adn appauvri en unites de repetition WO2001029252A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU77890/00A AU7789000A (en) 1999-10-18 2000-10-18 A method for the generation of repeat-depleted dna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99120618.6 1999-10-18
EP99120618 1999-10-18

Publications (2)

Publication Number Publication Date
WO2001029252A2 WO2001029252A2 (fr) 2001-04-26
WO2001029252A3 true WO2001029252A3 (fr) 2002-01-31

Family

ID=8239219

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010268 WO2001029252A2 (fr) 1999-10-18 2000-10-18 Procede permettant de produire de l'adn appauvri en unites de repetition

Country Status (2)

Country Link
AU (1) AU7789000A (fr)
WO (1) WO2001029252A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003901671A0 (en) * 2003-04-02 2003-05-01 The University Of Adelaide Comparative genomic hybridization
CN106222250A (zh) * 2016-07-22 2016-12-14 厦门艾德生物医药科技股份有限公司 一种fish探针的制备方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5824479A (en) * 1995-05-22 1998-10-20 Forschungszentrum Fur, Umwelt Und Gesundheit Gmbh Inter-line-PCR
US5861245A (en) * 1990-10-15 1999-01-19 Stratagene & California Institute Of Biological Research Arbitrarily primed polymerase chain reaction method for fingerprinting genomes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861245A (en) * 1990-10-15 1999-01-19 Stratagene & California Institute Of Biological Research Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5824479A (en) * 1995-05-22 1998-10-20 Forschungszentrum Fur, Umwelt Und Gesundheit Gmbh Inter-line-PCR

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOLZER A ET AL: "COMPLETE SET OF REPEAT-DEPLETED, PCR-AMPLIFIABLE, HUMAN CHROMOSOME-SPECIFIC PAINTING PROBES", CYTOGENETICS AND CELL GENETICS,CH,BASEL, vol. 84, no. 3/04, 1999, pages 233 - 240, XP000907217, ISSN: 0301-0171 *
CHEN-LIU L W ET AL: "SELECTION OF HYBRIDS BY AFFINITY CAPTURE (SHAC): A METHOD FOR THE GENERATION OF CDNAS ENRICHED IN SEQUENCES FORM A SPECIFIC CHROMOSOME REGION", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 30, no. 2, 20 November 1995 (1995-11-20), pages 388 - 392, XP002051194, ISSN: 0888-7543 *
CRAIG J M ET AL: "REMOVAL OF REPETITIVE SEQUENCES FROM FISH PROBES USING PCR-ASSISTEDAFFINITY CHROMATOGRAPHY", HUMAN GENETICS,DE,BERLIN, vol. 100, no. 3/04, 1 September 1997 (1997-09-01), pages 472 - 476, XP002051198 *
MELTZER P S ET AL: "RAPID GENERATION OF REGION SPECIFIC PROBES BY CHROMOSOME MICRODISSECTION AND THEIR APPLICATION", NATURE GENETICS,US,NEW YORK, NY, vol. 1, 1 April 1992 (1992-04-01), pages 24 - 28, XP000400731, ISSN: 1061-4036 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 13, 1992, pages 718 - 725, XP000199116, ISSN: 0888-7543 *

Also Published As

Publication number Publication date
WO2001029252A2 (fr) 2001-04-26
AU7789000A (en) 2001-04-30

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