WO2001029241A3 - Methods and vectors for site-specific recombination in plant cell plastids - Google Patents

Methods and vectors for site-specific recombination in plant cell plastids Download PDF

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Publication number
WO2001029241A3
WO2001029241A3 PCT/US2000/028620 US0028620W WO0129241A3 WO 2001029241 A3 WO2001029241 A3 WO 2001029241A3 US 0028620 W US0028620 W US 0028620W WO 0129241 A3 WO0129241 A3 WO 0129241A3
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WO
WIPO (PCT)
Prior art keywords
methods
specific recombination
plant cell
site
vectors
Prior art date
Application number
PCT/US2000/028620
Other languages
French (fr)
Other versions
WO2001029241A2 (en
Inventor
Jeffrey M Staub
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Priority to BR0014858-0A priority Critical patent/BR0014858A/en
Priority to AU10907/01A priority patent/AU1090701A/en
Priority to CA002387876A priority patent/CA2387876A1/en
Priority to EP00972211A priority patent/EP1222296A2/en
Publication of WO2001029241A2 publication Critical patent/WO2001029241A2/en
Publication of WO2001029241A3 publication Critical patent/WO2001029241A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Novel compositions and methods useful for genetic engineering of plant cells are provided. In particular, plastid constructs comprising recombining sites are provided. Such constructs find use in methods for site specific recombination in plant cell plastids. Plant cells and plants comprising the constructs described herein, as well as plant cells and plants produced by the methods of the present invention are of interest.
PCT/US2000/028620 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids WO2001029241A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BR0014858-0A BR0014858A (en) 1999-10-15 2000-10-16 Methods and vectors for recombination of specific site in plant cell plastids
AU10907/01A AU1090701A (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids
CA002387876A CA2387876A1 (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids
EP00972211A EP1222296A2 (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US15987699P 1999-10-15 1999-10-15
US60/159,876 1999-10-15
US22554200P 2000-08-16 2000-08-16
US60/225,542 2000-08-16

Publications (2)

Publication Number Publication Date
WO2001029241A2 WO2001029241A2 (en) 2001-04-26
WO2001029241A3 true WO2001029241A3 (en) 2001-11-29

Family

ID=26856401

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028620 WO2001029241A2 (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids

Country Status (5)

Country Link
EP (1) EP1222296A2 (en)
AU (1) AU1090701A (en)
BR (1) BR0014858A (en)
CA (1) CA2387876A1 (en)
WO (1) WO2001029241A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8937216B2 (en) 2007-03-09 2015-01-20 Monsanto Technology Llc Method of meristem excision and transformation

Families Citing this family (20)

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Publication number Priority date Publication date Assignee Title
ES2558160T3 (en) 1998-12-09 2016-02-02 Phyton Holdings, Llc Glycoproteins that have human-type glycosylation
ES2288882T3 (en) 1999-10-26 2008-02-01 Plant Research International B.V. GLAMMOSILATION OF MAMMER TYPE IN PLANTS.
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
JP2003530888A (en) * 2000-04-20 2003-10-21 ビーティージー・インターナショナル・リミテッド Transgenic plants
WO2002057468A2 (en) 2001-01-19 2002-07-25 The Dow Chemical Company Method for secretory production of glycoprotein having human-type sugar chain using plant cell
EP1461439A1 (en) * 2001-12-20 2004-09-29 Sungene GmbH & Co. KGaA Method for the transformation of vegetable plastids
WO2003054189A2 (en) * 2001-12-20 2003-07-03 Sungene Gmbh & Co. Kgaa Methods for the transformation of vegetal plastids
JP5350573B2 (en) 2002-03-19 2013-11-27 スティヒティング ディーンスト ランドバウクンディフ オンデルズーク GNTIII expression in plants
AU2003219418B2 (en) 2002-03-19 2008-02-28 Stichting Dienst Landbouwkundig Onderzoek Optimizing glycan processing in plants
WO2003106415A2 (en) 2002-06-13 2003-12-24 Diversa Corporation Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid
CL2003002461A1 (en) 2002-11-27 2005-01-07 Dow Chemical Company Agroscien IMMUNOGLOBULIN THAT UNDERSTANDS AT LEAST ONE AFUCOSILATED GLICAN, COMPOSITION THAT CONTAINS IT, NUCLEOTIDIC SEQUENCE AND VECTOR THAT UNDERSTANDS IT, PROCEDURE TO PRODUCE IMMUNOGLOBULIN SAID IN PLANTS.
EP1606386A4 (en) * 2003-03-03 2007-07-18 Univ Rutgers Removal of plastid sequences by transiently expressed site-specific recombinases
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
GB2447416B (en) * 2006-12-15 2010-08-25 Uni I Stavanger Methods and vectors for transformation of plastids and marker excision using site-specific recombination
CA2679503A1 (en) 2007-03-01 2008-09-04 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US8829276B2 (en) 2007-04-17 2014-09-09 Stichting Dienst Landbouwkundig Onderzoek Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
WO2011011693A1 (en) 2009-07-23 2011-01-27 Chromatin, Inc. Sorghum centromere sequences and minichromosomes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997041228A2 (en) * 1996-05-01 1997-11-06 Pioneer Hi-Bred International, Inc. Use of the green fluorescent protein as a screenable marker for plant transformation
WO2001021768A1 (en) * 1999-09-21 2001-03-29 Rutgers, The State University Of New Jersey Site-specific recombination system to manipulate the plastid genome of higher plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997041228A2 (en) * 1996-05-01 1997-11-06 Pioneer Hi-Bred International, Inc. Use of the green fluorescent protein as a screenable marker for plant transformation
WO2001021768A1 (en) * 1999-09-21 2001-03-29 Rutgers, The State University Of New Jersey Site-specific recombination system to manipulate the plastid genome of higher plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DALE E C ET AL: "GENE TRANSFER WITH SUBSEQUENT REMOVAL OF THE SELECTION GENE FROM THE HOST GENOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 88, 1 December 1991 (1991-12-01), pages 10558 - 10562, XP002043803, ISSN: 0027-8424 *
DALE ET AL: "Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 91, 1990, pages 79 - 85, XP002094597, ISSN: 0378-1119 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8937216B2 (en) 2007-03-09 2015-01-20 Monsanto Technology Llc Method of meristem excision and transformation
US9006513B2 (en) 2007-03-09 2015-04-14 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
US11566252B2 (en) 2007-03-09 2023-01-31 Monsanto Technology, Llc Preparation and use of plant embryo explants for transformation

Also Published As

Publication number Publication date
BR0014858A (en) 2002-07-16
WO2001029241A2 (en) 2001-04-26
EP1222296A2 (en) 2002-07-17
AU1090701A (en) 2001-04-30
CA2387876A1 (en) 2001-04-26

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