WO2001029205A3 - Method for genetic manipulation - Google Patents

Method for genetic manipulation Download PDF

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Publication number
WO2001029205A3
WO2001029205A3 PCT/IB2000/001622 IB0001622W WO0129205A3 WO 2001029205 A3 WO2001029205 A3 WO 2001029205A3 IB 0001622 W IB0001622 W IB 0001622W WO 0129205 A3 WO0129205 A3 WO 0129205A3
Authority
WO
WIPO (PCT)
Prior art keywords
minos
mrna
transposase
injected
transposon
Prior art date
Application number
PCT/IB2000/001622
Other languages
French (fr)
Other versions
WO2001029205A2 (en
Inventor
Charalambos Savakis
Original Assignee
Charalambos Savakis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9924721.5A external-priority patent/GB9924721D0/en
Priority claimed from GB0016068A external-priority patent/GB0016068D0/en
Application filed by Charalambos Savakis filed Critical Charalambos Savakis
Priority to AU12923/01A priority Critical patent/AU1292301A/en
Publication of WO2001029205A2 publication Critical patent/WO2001029205A2/en
Publication of WO2001029205A3 publication Critical patent/WO2001029205A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Capped RNA corresponding to the spliced transcript of the Minos transposable element has been synthesised in vitro and shown to be active as a source of transposase for Minos transposon mobilisation. When co-injected into Drosophila and Medfly embryos, Minos mRNA can mobilise homologous transposons from plasmids into germline chromosomes. Injected mRNA is superior to injected plasmids expressing the transposase as a source of the enzyme, resulting in several-fold increases in transformation efficiencies. In a rapid test for mRNA activity, Minos mRNA has been shown to catalyse excision of a Minos transposon from plasmid DNA in Medfly embryos. High frequency mobilisation of a Minos transposon from the X chromosome into autosomes has also been demonstrated after injections of Minos transposase mRNA into pre-blastoderm Drosophila embryos. The rates of transposition (approximately 30 %) suggest that this is a powerful system for genetic manipulation.
PCT/IB2000/001622 1999-10-19 2000-10-19 Method for genetic manipulation WO2001029205A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU12923/01A AU1292301A (en) 1999-10-19 2000-10-19 Method for genetic manipulation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9924721.5 1999-10-19
GBGB9924721.5A GB9924721D0 (en) 1999-10-19 1999-10-19 Protein production system
GB0016068A GB0016068D0 (en) 2000-06-30 2000-06-30 Method for genetic manipulation
GB0016068.9 2000-06-30

Publications (2)

Publication Number Publication Date
WO2001029205A2 WO2001029205A2 (en) 2001-04-26
WO2001029205A3 true WO2001029205A3 (en) 2001-09-07

Family

ID=26244571

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/001622 WO2001029205A2 (en) 1999-10-19 2000-10-19 Method for genetic manipulation

Country Status (2)

Country Link
AU (1) AU1292301A (en)
WO (1) WO2001029205A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003209453A1 (en) * 2002-03-05 2003-09-16 Arrow Therapeutics Limited Transposon
WO2003097826A1 (en) * 2002-05-16 2003-11-27 Minos Biosystems Limited Use of minos in functional genomics
FR2850668B1 (en) * 2003-01-31 2005-04-08 Centre Nat Rech Scient MOBILE GENETIC ELEMENTS BELONGING TO THE MARINER FAMILY IN HYDROTHERMAL EUCARYOTES

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614398A (en) * 1994-11-18 1997-03-25 The University Of Maryland Biotechnology Institute Gene transfer system for insects
WO1998040510A1 (en) * 1997-03-11 1998-09-17 Regents Of The University Of Minnesota Dna-based transposon system for the introduction of nucleic acid into dna of a cell
US5840865A (en) * 1992-09-14 1998-11-24 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
WO1999009817A1 (en) * 1997-08-22 1999-03-04 Biotechnology And Biological Sciences Research Council Use of mariner transposan in the production of transgenic animals
WO1999025817A2 (en) * 1997-11-13 1999-05-27 Regents Of The University Of Minnesota Tc1-based transposon vectors
EP0955364A2 (en) * 1998-04-27 1999-11-10 Institute For Molecular Biology And Biotechnology/Forth Eukaryotic transposable element

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840865A (en) * 1992-09-14 1998-11-24 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
US5614398A (en) * 1994-11-18 1997-03-25 The University Of Maryland Biotechnology Institute Gene transfer system for insects
WO1998040510A1 (en) * 1997-03-11 1998-09-17 Regents Of The University Of Minnesota Dna-based transposon system for the introduction of nucleic acid into dna of a cell
WO1999009817A1 (en) * 1997-08-22 1999-03-04 Biotechnology And Biological Sciences Research Council Use of mariner transposan in the production of transgenic animals
WO1999025817A2 (en) * 1997-11-13 1999-05-27 Regents Of The University Of Minnesota Tc1-based transposon vectors
EP0955364A2 (en) * 1998-04-27 1999-11-10 Institute For Molecular Biology And Biotechnology/Forth Eukaryotic transposable element

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ARCA B ET AL: "Mobilization of a Minos transposon in Drosophila melanogaster chromosomes and chromatid repair by heteroduplex formation", GENETICS, vol. 145, no. 2, February 1997 (1997-02-01), pages 267 - 279, XP000990922 *
FADOOL J ET AL: "Transposition of the mariner element from Drosophila mauritiana in zebrafish", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 95, no. 9, 28 April 1998 (1998-04-28), pages 5182 - 5186, XP002164305 *
LOUKERIS T ET AL: "Introduction of the transposable element Minos into the germ line of Drosophila melanogaster", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 92, no. 21, 10 October 1995 (1995-10-10), pages 9485 - 9489, XP002164306 *
LOUKERIS T G ET AL: "GENE TRANSFER INTO THE MEDFLY, CERATITIS CAPITATA, WITH A DROSOPHILA HYDEI TRANSPOSABLE ELEMENT", SCIENCE,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,,US, vol. 270, no. 5244, 22 December 1995 (1995-12-22), pages 2002 - 2005, XP000941764, ISSN: 0036-8075 *
PLASTERK R ET AL: "Resident aliens: The Tc1/mariner superfamily of transposable elements", TRENDS IN GENETICS, vol. 15, no. 8, August 1999 (1999-08-01), pages 326 - 332, XP004172552 *

Also Published As

Publication number Publication date
WO2001029205A2 (en) 2001-04-26
AU1292301A (en) 2001-04-30

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