WO2001028592A1 - Cobalamin conjugates useful as imaging agents and as antitumor agents - Google Patents
Cobalamin conjugates useful as imaging agents and as antitumor agents Download PDFInfo
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- WO2001028592A1 WO2001028592A1 PCT/US2000/010097 US0010097W WO0128592A1 WO 2001028592 A1 WO2001028592 A1 WO 2001028592A1 US 0010097 W US0010097 W US 0010097W WO 0128592 A1 WO0128592 A1 WO 0128592A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
- A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
- A61K51/04—Organic compounds
- A61K51/041—Heterocyclic compounds
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/55—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound the modifying agent being also a pharmacologically or therapeutically active agent, i.e. the entire conjugate being a codrug, i.e. a dimer, oligomer or polymer of pharmacologically or therapeutically active compounds
- A61K47/551—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound the modifying agent being also a pharmacologically or therapeutically active agent, i.e. the entire conjugate being a codrug, i.e. a dimer, oligomer or polymer of pharmacologically or therapeutically active compounds one of the codrug's components being a vitamin, e.g. niacinamide, vitamin B3, cobalamin, vitamin B12, folate, vitamin A or retinoic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
- A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
- A61K51/04—Organic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
- A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
- A61K51/04—Organic compounds
- A61K51/0474—Organic compounds complexes or complex-forming compounds, i.e. wherein a radioactive metal (e.g. 111In3+) is complexed or chelated by, e.g. a N2S2, N3S, NS3, N4 chelating group
- A61K51/0485—Porphyrins, texaphyrins wherein the nitrogen atoms forming the central ring system complex the radioactive metal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Definitions
- Cancer is a general term frequently used to indicate any of the various types of malignant neoplasms (i.e., abnormal tissue that grows by cellular proliferation more rapidly than normal), most of which invade surrounding tissue, may metastasize to several sites, are likely to recur after attempted removal, and causes death unless adequately treated.
- malignant neoplasms i.e., abnormal tissue that grows by cellular proliferation more rapidly than normal
- Most of which invade surrounding tissue may metastasize to several sites, are likely to recur after attempted removal, and causes death unless adequately treated.
- Stedm n's Medical Dictionary 25th Edition Illustrated, Williams & Wilkins, 1990.
- Approximately 1.2 million Americans are diagnosed with cancer each year, 8,000 of which are children.
- 500,000 Americans die from cancer each year in the United States alone.
- lung and prostate cancer are the top cancer killers for men while lung and breast cancer are the top cancer killers for women.
- CNN Cancer Facts http://www.cnn.com/HEALTH/951 l/conquer_cancer/facts/index.html, page 2 of 2, July 18, 1999.
- cancer Although a variety of approaches to cancer therapy (e.g., surgical resection, radiation therapy, and chemotherapy) have been available and commonly used for many years, cancer remains one of the leading causes of death in the world. This is due in part to the therapies themselves causing significant toxic side-effects as well as the re-emergence of the deadly disease. The toxicity associated with conventional cancer chemotherapy is due primarily to a lack of specificity of the chemotherapeutic agent.
- anti-cancer drugs by themselves typically do not distinguish between malignant and normal cells. As a result, anti-cancer drugs are absorbed by both cell types. Thus, conventional chemotherapeutic agents not only destroy diseased cells, but also destroy normal, healthy cells. To overcome this limitation, therapeutic strategies that increase the specificity, increase the efficacy, as well as reduce the toxicity of anti-cancer drugs are being explored.
- One such strategy that is being aggressively pursued is drug targeting.
- An objective of drug targeting is to deliver drugs to a- specific site of action through a carrier system. Such targeting achieves at least two major aims of drug delivery. The first is to deliver the maximum dose of therapeutic agent to diseased cells. The second is the avoidance of uptake by normal, healthy cells.
- targeted drug delivery systems result in enhancing drug accumulation in tumors while decreasing exposure to susceptible healthy tissues. As such, the efficacy is increased while the toxicity is decreased.
- porphyrins Two classes of compounds with a propensity for localizing in malignant tumors are the porphyrins and the related phthalocyanines.
- the biochemical basis by which these compounds achieve elevated concentration in malignant tumors is unknown, but this observation has served as the rationale for the use of hematoporphyrin derivatives in the photodyamic therapy of cancer (Dougherty, T.J. et al., Porphyrin Photosensitization, 3-13, New York: Plenum Publishing Corp. (1981)).
- Figure 1 The structure of these various forms is shown in Figure 1, wherein X is CN, OH, CH 3 or adenosyl, respectively.
- cobalamin will be used to refer to all of the molecule except the X group.
- the fundamental ring system without cobalt (Co) or side chains is called corrin and the octadehydrocorrin is called corrole.
- Figure 1 is adapted from The Merck Index, Merck & Co. (11th ed. 1989), wherein X is above the plane defined by the corrin ring and the nucleotide is below the plane of the ring.
- the corrin ring has attached seven amidoalkyl (H 2 NC(O)Alk) substituents, at the 2, 3, 7, 8, 13, 18 and 23 positions, which can be designated a-g respectively. See D.L. Anton et al., T Amer. Chem. Soc. 102, 2215 (1980). The 2, 3, 7, 8, and 13 positions are shown in Figure 1 as positions a-e, respectively.
- methylcobalamin is directly involved with methionine synthesis and indirectly involved in the synthesis of thymidylate and DNA, it is not surprising that methylcobalamin as well as Cobalt-57-cyanocobalamin have also been shown to have increased uptake in rapidly dividing tissue (for example, see, B.A. Cooper et al., Nature, 121, 393 (1961); H. Flodh, Acta Radiol. Snpp 2S4, 55 (1968); L. Bloomquist et al., Experientia. 25, 294 (1969)). Additionally, upregulation in the number of transcobalamin II receptors has been demonstrated in several malignant cell lines during their accelerated thymidine incorporation and DNA synthesis (see, J.
- bioconjugates i.e., conjugates containing a bioactive agent and an organocobalt complex in which the bioactive agent is covalently bound directly or indirectly, via a spacer, to the cobalt atom.
- the organocobalt complex can be cobalamin and the bioactive agent can be a chemotherapeutic agent.
- chemotherapeutic agent only one bioactive agent (i.e., chemotherapeutic agent) is attached to the organocobalt complex (i.e., cobalamin) and the attachment is to the cobalt atom (i.e., the 6-position of cobalamin).
- the bioactive agent is released from the bioconjugate by the cleavage of the weak covalent bond between the bioactive agent and the cobalt atom as a result of normal displacement by cellular nucleophiles or enzymatic action, or by application of an external signal (e.g., light, photoexcitation, ultrasound, or the presence of a magnetic filed).
- an external signal e.g., light, photoexcitation, ultrasound, or the presence of a magnetic filed.
- chemotherapeutic agents that have improved specificity (i.e., localize in tumor cells in high concentration compared to normal cells), or efficacy, and for chemotherapeutic agents which can selectively target cancer cells.
- cobalamin conjugates i.e., conjugates of Vitamin B 12 and a chemotherapeutic agent
- the cobalamin conjugates have a low toxicity, a high activity against diseased cells, and a high specificity (i.e., they localize in tumor cells in a higher concentration than in normal cells).
- the present invention provides a compound (i.e., cobalamin conjugate of the present invention) wherein a residue of a compound of formula I ( Figure 1) is linked directly or by a linker to a residue of one or more chemotherapeutic agents; wherein X is CN, OH, CH 3 , or adenosyl; or a pharmaceutically acceptable salt thereof.
- the present invention also provides a compound (i.e., a cobalamin conjugate of the present invention) wherein a residue of a compound of formula I ( Figure 1) is linked directly or by a linker to a residue of a chemotherapeutic agent through the 6-position and wherein a residue of the compound of formula I is linked directly or by a linker to a residue of one or more additional chemotherapeutic agents; or a pharmaceutically acceptable salt thereof.
- a compound i.e., a cobalamin conjugate of the present invention
- the present invention also provides a compound (i.e., cobalamin conjugate of the present invention) of formula II
- X is CN, OH, CH 3 , adenosyl, or LL- TT wherein LL is a linker or is absent and TT is a residue of a chemotherapeutic agent; L is a linker or absent; and T is a residue of a chemotherapeutic agent; or a pharmaceutically acceptable salt thereof.
- the present invention also provides a compound (i.e., cobalamin conjugate of the present invention) of formula II
- X is LL-TT wherein LL is a linker or is absent and TT is a residue of a chemotherapeutic agent; L is a linker or absent; and T is a residue of a chemotherapeutic agent; or a pharmaceutically acceptable salt thereof.
- the present invention also provides a compound (i.e., cobalamin conjugate of the present invention) of formula III:
- X is CN, OH, CH 3 , adenosyl, or ZZ-TT wherein ZZ is a linker or is absent and TT is a residue of a chemotherapeutic agent; Z is -N(R)-, -O-, -S-, or absent wherein R is H or (C--C 6 )alkyl; and T is a residue of a chemotherapeutic agent; or a pharmaceutically acceptable salt thereof.
- the present invention also provides a compound (i.e., cobalamin conjugate of the present invention) of formula III: X
- X is LL-TT wherein LL is a linker or is absent and TT is a residue of a chemotherapeutic agent
- Z is -N(R)-, -O-, -S-, or absent, wherein R is H or (C,- C 6 )alkyl
- T is a residue of a chemotherapeutic agent; or a pharmaceutically acceptable salt thereof.
- the present invention also provides a compound wherein a residue of a compound of formula I ( Figure 1) is linked directly or by a linker to a residue of one or more chemotherapeutic agents; wherein X is CN, OH, CH 3 , or adenosyl; wherein the compound of formula I is also linked directly or by a linker to a detectable radionuclide; or a pharmaceutically acceptable salt thereof.
- the present invention also provides a pharmaceutical composition comprising a cobalamin conjugate of the present invention, or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier.
- the present invention also provides a method of treating a tumor in a mammal in need of such treatment comprising administering to the mammal an effective amount of a cobalamin conjugate of the present invention, or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier.
- the invention also provides a method for imaging a tumor in a mammal in need of such imaging comprising administering to the mammal a detectable amount of a cobalamin conjugate of the present invention; and detecting the presence of the compound.
- the invention also provides a compound of the present invention for use in medical therapy or diagnosis.
- the invention also provides the use of a compound of the present invention for the manufacture of a medicament for imaging a tumor in a mammal (e.g., a human).
- the invention also provides the use of a compound of the present invention for the manufacture of a medicament for treating a tumor in a mammal (e.g., a human).
- the invention also provides intermediates disclosed herein that are useful in the preparation of the compounds of the present invention as well as synthetic methods useful for preparing the compounds of the invention.
- the cobalamin conjugate of the present invention has several characteristics which make it an attractive in vivo targeting agent.
- Vitamin B 12 is water soluble, has no known toxicity, and in excess is excreted by glomerular filtration.
- the uptake of vitamin B 12 can potentially be manipulated by the administration of nitrous oxide and other pharmacological agents (D. Swanson et al., Pharmaceuticals in Medical Imaging, MacMillan Pub. Co., NY (1990) at pages 621-628).
- Figure 1 illustrates a compound of formula I, wherein X is CN, OH, CH 3 , adenosyl or a residue of a chemotherapeutic agent.
- the compound of formula I can be cyanocobalamin (X is CN), hydroxocobalamin (X is OH), methylcobalamin (X is CH 3 ), or adenosylcobalamin (X is adenosyl).
- the compound of formula I can be a cobalamin conjugate (X is a residue of a chemotherapeutic agent or X is a linker linked to a residue of a chemotherapeutic agent).
- Figure 2 illustrates a proposed synthesis of a compound wherein a residue of a compound of formula I is linked to linker, which is linked to a residue of a chemotherapeutic agent.
- halo is fiuoro, chloro, bromo, or iodo.
- Alkyl, alkoxy, alkenyl, alkynyl, etc. denote both straight and branched groups; but reference to an individual radical such as "propyl” embraces only the straight chain radical, a branched chain isomer such as "isopropyl” being specifically referred to.
- Aryl denotes a phenyl radical or an ortho-fused bicyclic carbocyclic radical having about nine to ten ring atoms in which at least one ring is aromatic.
- radicals, substituents, and ranges are for illustration only; they do not exclude other defined values or other values within defined ranges for the radicals and substituents. It is appreciated that those skilled in the art will recognize that compounds of the present invention having a chiral center may exist in and be isolated in optically active and racemic forms. Some compounds may exhibit polymorphism.
- the present invention encompasses any racemic, optically-active, polymorphic, or stereoisomeric form, or mixtures thereof, of a compound of the invention, which possess the useful properties described herein, it being well known in the art how to prepare optically active forms (for example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase) and how to determine antitumor activity using the standard tests described herein, or using other similar tests which are well known in the art.
- (C*-C 6 )alkyl can be methyl, ethyl, propyl, isopropyl, butyl, iso-butyl, sec-butyl, pentyl, 3-pentyl, or hexyl.
- (C 2 -C 6 )alkenyl can be vinyl, allyl, 1 -propenyl, 2- propenyl, 1-butenyl, 2-butenyl, 3-butenyl, 1,-pentenyl, 2-pentenyl, 3-pentenyl, 4- pentenyl, 1- hexenyl, 2-hexenyl, 3-hexenyl, 4-hexenyl, or 5-hexenyl.
- (C 2 -C 6 )alkynyl can be ethynyl, 1-propynyl, 2- propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-pentynyl, 2-pentynyl, 3-pentynyl, 4-pentynyl, 1- hexynyl, 2-hexynyl, 3-hexynyl, 4-hexynyl, or 5-hexynyl.
- aryl can be phenyl, indenyl, or naphthyl.
- (C 3 -C 8 )cycloalkyl can be cyclopropyl, cyclobutyl, cyclcopentyl, cyclohexyl, cycloheptyl or cyclooctyl.
- adenosyl is an adenosine radical in which any synthetically feasible atom or group of atoms have been removed, thereby providing an open valence. Synthetically feasible atoms which may be removed include the hydrogen atom of the hydroxy group at the 5' position. Accordingly, adenosyl can conveniently be attached to the 6-position (i.e., the position occupied by X in the compound of formula I) of a compound of formula I via the 5' position of adenosyl.
- a "residue of a compound of formula I" is a radical of a compound of formula I having an open valence. Any synthetically feasible atom or atoms of the compound of formula I can be removed to provide the open valence, provided the resulting compound is able to localize in or near a tumor. Based on the linkage that is desired, one skilled in the art can select suitably functionalized starting materials that can be derived from a compound of formula I using procedures that are known in the art. For example, suitable atoms that can be removed include the NH 2 group of the a-carboxamide
- NH 2 group of the b-carboxamide (illustrated in figure 1), the NH 2 group of the d-carboxamide (illustrated in figure 1), the NH 2 group of the e carboxamide (illustrated in figure 1), the hydrogen atom of the hydroxy group at the 3' position of the sugar, and the hydrogen atom of the CH 2 OH group at the 5' position of the sugar ring may be removed.
- X at the 6- position (illustrated in figure 1) can be removed to provide an open valence to link a first chemotherapeutic agent.
- a "residue of a chemotherapeutic agent” is a radical of a chemotherapeutic agent having an open valence. Any synthetically feasible atom or atoms of the chemotherapeutic agent may be removed to provide the open valence, provided the bioactivity of the agent is retained when administered as a conjugate of the invention. In addition, the residue of the chemotherapeutic agent does not comprise a radionuclide. Based on the linkage that is desired, one skilled in the art can select suitably functionalized starting materials that can be derived from a chemotherapeutic agent using procedures that are known in the art.
- a "residue of doxorubicin or paclitaxel” is a radical of doxorubicin or a radical of paclitaxel having an open valence formed by removing a substituent (i.e., atom or group of atoms) from doxorubicin or by removing a substituent (i.e., atom or group of atoms) from paclitaxel.
- a substituent i.e., atom or group of atoms
- Any synthetically feasible atom or atoms of doxorubicin or paclitaxel may be removed to provide the open valence, provided useful bioactivity is retained when administered as a conjugate of the invention.
- suitably functionalized starting materials that can be derived from doxorubicin or paclitaxel using procedures that are known in the art.
- amino acid is a natural amino acid residue (e.g. Ala, Arg, Asn, Asp, Cys, Glu, Gin, Gly, His, Hyl, Hyp, He, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and Val) in D or L form, as well as unnatural amino acid (e.g.
- phosphoserine phosphothreonine; phosphotyrosine; hydroxyproline; gamma-carboxyglutamate; hippuric acid; octahydroindole-2-carboxylic acid; statine; l,2,3,4,-tetrahydroisoquinoline-3-carboxylic acid; penicillamine; ornithine; citruline; ⁇ -methyl-alanine; para-benzoylphenylalanine; phenylglycine; propargylglycine; sarcosine; and tert-butylglycine) residue having one or more open valences.
- the term also comprises natural and unnatural amino acids bearing amino protecting groups (e.g.
- acetyl, acyl, trifluoroacetyl, or benzyloxycarbonyl as well as natural and unnatural amino acids protected at carboxy with protecting groups (e.g. as a (C r C 6 )alkyl, phenyl or benzyl ester or amide).
- protecting groups e.g. as a (C r C 6 )alkyl, phenyl or benzyl ester or amide.
- suitable amino and carboxy protecting groups are known to those skilled in the art (See for example, T.W. Greene, Protecting Groups In Organic Synthesis; Wiley: New York, 1981; D. Voet, Biochemistry. Wiley: New York, 1990; L. Stryer, Biochemistry, (3rd Ed.), W.H. Freeman and Co.: New York, 1975; J. March, Advanced Organic Chemistry, Reactions,
- the amino or carboxy protecting group can comprise a radionuclide (e.g., Fluorine-18, Iodine-123, or Iodine-124).
- a radionuclide e.g., Fluorine-18, Iodine-123, or Iodine-124.
- a "peptide” is a sequence of 2 to 25 mino acids (e.g. as defined herein) or peptidic residues.
- the sequence may be linear or cyclic.
- a cyclic peptide can be prepared or may result from the formation of disulfide bridges between two cysteine residues in a sequence.
- a peptide can be linked through the carboxy terminus, the amino terminus, or through any other convenient point of attachment, such as, for example, through the sulfur of a cysteine.
- a peptide comprises 2 to about 20, 2 to about 15, or 2 to about 12 amino acids.
- Peptide derivatives can be prepared as disclosed in U.S.
- the peptide can be poly-L-lysine, poly-L-glutamic acid, poly-L-aspartic acid, poly-L-histidine, poly-L-ornithine, poly-L-serine, poly-L-threonine, poly-L-tyrosine, poly-L-lysine-L-phenylalanine or poly-L- lysine-L-tyrosine.
- chemotherapeutic agent is a compound that has biological activity against one or more forms of cancer and can be linked to the residue of a compound of formula I without losing its anticancer activity.
- Suitable chemotherapeutic agents include antineoplasts.
- Representative antineoplasts include adjuncts, androgen inhibitors, antibiotic derivatives, antiestrogens, antimetabolites, cytotoxic agents, hormones, immunomodulators, nitrogen mustard derivatives and steroids. Physicians' Desk Reference, 50th Edition, 1996.
- adjuncts include levamisole, gallium nitrate, granisetron, sargramostim strontium- 89 chloride, filgrastim, pilocarpine, dexrazoxane, and ondansetron. Physicians' Desk Reference, 50th Edition, 1996.
- Representative androgen inhibitors include flutamide and leuprolide acetate. Physicians' Desk Reference. 50th Edition, 1996.
- antibiotic derivatives include doxorubicin, bleomycin sulfate, daunorubicin, dactinomycin, and idarubicin.
- Representative antiestrogens include tamoxifen citrate and analogs thereof. Physicians' Desk Reference, 50th Edition, 1996. Additional antiestrogens include nonsteroidal antiestrogens such as toremifene, droloxifene and roloxifene. Magarian et al., Current Medicinal Chemistry, 1994, Vol. 1, No. 1.
- Representative antimetabolites include fiuorouracil, fludarabine phosphate, floxuridine, interferon alfa-2b recombinant, methotrexate sodium, plicamycin, mercaptopurine, and thioguanine. Physicians' Desk Reference, 50th Edition, 1996.
- cytotoxic agents include doxorubicin, carmustine [BCNU], lomustine [CCNU], cytarabine USP, cyclophosphamide, estramucine phosphate sodium, altretamine, hydroxyurea, ifosfamide, procarbazine, mitomycin, busulfan, cyclophosphamide, mitoxantrone, carboplati, cisplati, cisplatin, interferon alfa-2a recombinant, paclitaxel, teniposide, and sfreptozoci. Physicians' Desk Reference. 50th Edition, 1996.
- hormones include medroxyprogesterone acetate, estradiol, megestrol acetate, octreotide acetate, diethylstilbestrol diphosphate, testolactone, and goserelin acetate. Physicians' Desk Reference. 50th Edition, 1996.
- Representative immunodilators include aldesleukin. Physicians' Desk Reference. 50th Edition, 1996.
- nitrogen mustard derivatives include melphalan, chlorambucil, mechlorethamine, and thiotepa. Physicians' Desk Reference, 50th Edition, 1996.
- steroids include betamethasone sodium phosphate and betamethasone acetate. Physicians' Desk Reference. 50th Edition, 1996.
- the chemotherapeutic agent can be an antineoplastic agent.
- the antineoplastic agent can be a cytotoxic agent.
- the cytotoxic agent can be paclitaxel or doxorubicin.
- chemotherapeutic agents include alkylating agents, antimitotic agents, plant alkaloids, biologicals, topoisomerase I inhibitors, topoisomerase II inhibitors, and synthetics.
- AntiCancer Agents by Mechanism http://www.dtp.nci.nih.gov/docs/cancer/searches/standard_mechanism_list.html, April 12, 1999; Approved Anti-Cancer Agents, http://www.ctep.info.nih.gov/handbook/HandBookText/fda_agen.htm, pages 1- 7, June 18, 1999; MCMP 61 1 Chemotherapeutic Drugs to Know.
- alkylating agents include asaley, AZQ, BCNU, busulfan, bisulphan, carboxyphthalatoplatinum, CBDCA, CCNU, CHIP, chlorambucil, chlorozotocin, cis -platinum, clomesone, cyanomorpholinodoxorubicin, cyclodisone, cyclophosphamide, dianhydrogalactitol, fluorodopan, hepsulfam, hycanthone, iphosphamide, melphalan, methyl CCNU, mitomycin C, mitozolamide, nitrogen mustard, PCNU, piperazine, piperazinedione, pipobroman, porfiromycin, spirohydantoin mustard, streptozotocin, teroxirone, tetraplatin, thiotepa, triethylenemelamine, uracil nitrogen mustard, and Yoshi-864. AntiCancer Agents by Mechanism, http://dtp
- antimitotic agents include allocolchicine, Halichondrin B, colchicine, colchicine derivatives, dolastatin 10, maytansine, rhizoxin, paclitaxel derivatives, paclitaxel, thiocolchicine, trityl cysteine, vinblastine sulfate, and vincristine sulfate.
- Representative plant alkaloids include actinomycin D, bleomycin, L-asparaginase, idarubicin, vinblastine sulfate, vincristine sulfate, mitramycin, mitomycin, daunorubicin, VP- 16-213, VM-26, navelbine and taxotere. Approved Anti -Cancer Agents. http://ctep.info.nih.gov/handbook/HandBookText/fda_agent.htm, June 18, 1999.
- Representative biologicals include alpha interferon, BCG, G-CSF, GM-CSF, and interleukin-2.
- Approved Anti-Cancer Agents http://ctep.info.nih.gov/handbook/HandBookText/fda_agent.htm, June 18, 1999.
- Representative topoisomerase I inhibitors include camptothecin, camptothecin derivatives, and morpholinodoxorubicin.
- AntiCancer Agents by Mechanism http://dtp.nci.nih.gov/docs/cancer/searches/standard_mechanism_list.html, April 12, 1999.
- topoisomerase II inhibitors include mitoxantron, amonafide, m-AMSA, anthrapyrazole derivatives, pyrazoloacridine, bisantrene HCL, daunorubicin, deoxydoxorubicin, menogaril, N, N-dibenzyl daunomycin, oxanthrazole, rubidazone, VM-26 and VP-16.
- AntiCancer Agents by Mechanism http://dtp.nci.nih.gov/docs/cancer/searches/standard_mechanism_list.html, April 12, 1999.
- Representative synthetics include hydroxyurea, procarbazine, o,p'-DDD, dacarbazine, CCNU, BCNU, cis-diamminedichloroplatimun, mitoxantrone, CBDCA, levamisole, hexamethylmelamine, all-trans retinoic acid, gliadel and porfimer sodium. Approved Anti -Cancer Agents, http://ctep.info.nih.gov/handbook/HandBookText/fda_agen.htm, June 18, 1999.
- Such a linkage can be formed from suitably functionalised starting materials using synthetic procedures that are known in the art. Based on the linkage that is desired, one skilled in the art can select suitably functional starting materials that can be derived from a residue of a compound of formula I and from a given residue of a chemotherapeutic agent using procedures that are known in the art.
- the residue of the chemotherapeutic agent can be directly linked to any synthetically feasible position on the residue of a compound of formula I, provided if a residue of a chemotherapeutic agent is attached to a residue of a compound of formula I at the 6-position, the residue of a compound of formula I is attached to a residue of another chemotherapeutic agent or to a detectable radionuclide.
- Suitable points of attachment include, for example, the b- carboxamide, the d-carboxamide, and the e-carboxamide (illustrated in figure 1), as well as the 6-position (the position occupied by X in figure 1), and the 5'- hydroxy and the 3'-hydroxy groups on the 5-membered sugar ring, although other points of attachment are possible.
- U.S. Patent No. 5,739,313 discloses compounds (e.g., cyanocobalamin-b-(4-aminobutyl)amide, methylcobalamin-b- (4-aminobutyl)amide, and adenosylcobalamin-b-(4-aminobutyl)amide) that are useful intermediates for the preparation of compounds of the present invention.
- Compounds wherein the residue of a chemotherapeutic agent is directly linked to the 6-position of a compound of formula I can be prepared by reducing a corresponding Co (II) compound of formula I to form a nucleophilic Co (I) compound and treating this Co (I) compound with a residue of a chemotherapeutic agent (or a derivative thereof) comprising a suitable leaving group, such as a halide (e.g., a chloride).
- a suitable leaving group such as a halide (e.g., a chloride).
- the invention also provides compounds having more than one chemotherapeutic agent directly attached to a compound of formula I.
- the residue of a chemotherapeutic agent can be directly linked to a residue of the b-carboxamide of the compound of formula I and a residue of another chemotherapeutic agent can be directly linked to a residue of the d- carboxamide of the compound of formula I.
- the residue of a chemotherapeutic agent can be directly linked to the 6-position of the compound of formula I and a residue of another chemotherapeutic agent can be directly linked to a residue of the b-, d- or e-carboxamide of the compound of formula I.
- the residue of a chemotherapeutic agent can also be linked to the residue of a compound of formula I by a suitable linker.
- the structure of the linker is not crucial, provided it yields a compound of the invention which has an effective therapeutic index against the target cells, and which will localize in or near tumor molecules, which properties can be determined by those skilled in the art with assays that are known in the art.
- Suitable linkers include linkers that separate the residue of a compound of formula I and the chemotherapeutic agent by about 5 angstroms to about 200 angstroms, inclusive, in length.
- Other suitable linkers include linkers that separate the residue of a compound of formula I and the chemotherapeutic agent by about 5 angstroms to about 100 angstroms, as well as linkers that separate the residue of a compound of formula I and the chemotherapeutic agent by about 5 angstroms to about 50 angstroms, or by about 5 angstroms to about 25 angstroms.
- Suitable linkers are disclosed, for example, in U.S. Patent No. 5,735,313.
- the linker can be a divalent radical of the formula W-
- the linker can be a divalent radical, i.e., l, ⁇ -divalent radicals formed from a peptide or an amino acid.
- the peptide can comprise 2 to about 20 amino acids, 2 to about 15 amino acids, or 2 to about 12 amino acids.
- the peptide or amino acid can optionally be protected, as described herein.
- the peptide can be poly-L-lysine (i.e., [-
- poly-L-lysine contains about 5 to about 15 residues (i.e., m is between about 5 and about 15). More specifically, poly-L-lysine contains about 8 to about 11 residues (i.e., m is between about 8 and about 11).
- the peptide can be poly-L-glutamic acid, poly-L- aspartic acid, poly-L-histidine, poly-L-ornithine, poly-L-serine, poly-L- threonine, poly-L-tyrosine, poly-L-lysine-L-phenylalanine or poly-L-lysine-L- tyrosine.
- the linker can be prepared from 1,6-diaminohexane
- amide e.g., - N(R)
- Such a linkage can be formed from suitably functionalised starting materials using synthetic procedures that are known in the art. Based on the linkage that is desired, one skilled in the art can select suitably functional starting materials that can be derived from a residue of a compound of formula I and from a given residue of a chemotherapeutic agent using procedures that are known in the art.
- the linker can be linked to any synthetically feasible position on the residue of a compound of formula I, provided if a linker is attached to a residue of a compound of formula I at the 6-position, at least one residue of a chemotherapeutic agent is linked directly or by a linker to a residue of the compound of formula I at a position other than the 6-position (i.e., the position occupied by X in the compound of formula I).
- Suitable points of attachment include, for example, a residue of the b-carboxamide, a residue of the d- carboxamide, and a residue of the e-carboxamide, the 6-position, as well as a residue of the 5'-hydroxy group and a residue of the 3'-hydroxy group on the 5-membered sugar ring, although other points of attachment are possible.
- Compounds wherein the linker is linked to the 6-position of a compound of formula I can be prepared by preparing a nucleophilic Co (I) species as described herein above, and reacting it with a linker comprising a suitable leaving group, such as a halide (e.g. a chloride).
- the invention also provides compounds having more than one chemotherapeutic agent attached to a compound of formula I, each through a linker.
- the residue of a chemotherapeutic agent can conveniently be linked, through a linker, to a residue of the b-carboxamide of the compound of formula I and a residue of another chemotherapeutic agent can conveniently be linked, through a linker, to a residue of the d- or e-carboxamide of the compound of formula I.
- residue of a chemotherapeutic agent can conveniently be linked, through a linker, to the 6-position of the compound of formula I and a residue of another chemotherapeutic agent can conveniently be linked, through a linker, to a residue of the b-, d- or e-carboxamide of the compound of formula I.
- the invention also provides compounds having more than one chemotherapeutic agent attached to a compound of formula I, either directly or through a linker.
- the residue of a chemotherapeutic agent can conveniently be linked, either directly or through a linker, to a residue of the b- carboxamide of the compound of formula I and a residue of another chemotherapeutic agent can conveniently be linked, either directly or through a linker, to a residue of the d- or e-carboxamide of the compound of formula I.
- residue of a chemotherapeutic agent can conveniently be linked, either directly or through a linker, to the 6-position of the compound of formula I and a residue of another chemotherapeutic agent can conveniently be linked, either directly or through a linker, to a residue of the b-, d- or e-carboxamide of the compound of formula I.
- the invention provides a residue of a compound of formula I which is linked to one or more residues of a chemotherapeutic agent; and which is also linked, directly or by a linker, to one or more detectable chelating groups including one or more detectable radionuclides.
- the detectable chelating group can be linked to a residue of the compound of formula I by a linker.
- Suitable linkers are described herein.
- suitable points of attachment of the compound of formula I for the linker including the detectable chelating group are described herein.
- a detectable chelating group including a radionuclide can be linked, via a linker, to a residue of a compound of the formula I.
- the linker can be linked to any synthetically feasible position on the residue of a residue of a compound of formula I; provided the compound localizes in or near tumors.
- Suitable points of attachment include, for example, a residue of the b- carboxamide, a residue of the d-carboxamide, and a residue of the e- carboxamide, the 6-position, as well as a residue of the 5'-hydroxy group and a residue of the 3'-hydroxy group on the 5-membered sugar ring, although other points of attachment are possible.
- the invention also provides compounds having more than one detectable chelating group attached to a compound of formula I, each through a linker.
- the detectable chelating group can conveniently be linked, through a linker, to a residue of the b-carboxamide of the compound of formula I and another detectable chelating group can conveniently be linked, through a linker, to a residue of the d- or e-carboxamide of the compound of formula I.
- the detectable chelating group can conveniently be linked, through a linker, to the 6-position of the compound of formula I and another detectable chelating group can conveniently be linked, through a linker, to a residue of the b-, d- or e-carboxamide of the compound of formula I.
- the invention also provides compounds having more than one detectable radionuclide attached to a residue of the compound of formula I, either directly or through a linker.
- a “detectable chelating group” is a chelating group comprising a metallic radionuclide (e.g., a metallic radioisotope) capable of being detected in a diagnostic procedure in vivo or in vitro. Any suitable chelating group can be employed. Suitable chelating groups include those disclosed in U.S. Patent Number 5,739,313.
- the chelating group can be NTA, HEDTA, DCTA, RP414, MDP, DOTATOC, CDTA, HYNIC, EDTA, DTPA, TETA, DOTA, DOTMP, DCTA, 15N4, 9N3, 12N3, or MAG3 (or another suitable polyamino acid chelator), which are described herein below, or a phosphonate chelator (e.g. EDMT). More specifically, the chelating group can be DTPA.
- DTPA diethylenetriaminepentaacetic acid
- TETA is 1,4,8,11- tetraazacyclotetradecane-N,N ⁇ N",N"'-tetraacetic acid
- DOTA is 1,4,7,10- tetraazacyclododecane-N,N',N",N'"-tetraacetic acid
- 15N4 is 1,4,8,12- tetraazacyclopentadecane-N,N',N",N'"-tetraacetic acid
- 9N3 is 1,4,7- triazacyclononane-N,N',N"-triacetic acid
- 12N3 is 1,5,9-triazacyclododecane- N,N',N"-triacetic acid
- MAG3 is (N-[N-[N-[(benzoylthio) acetyl]glycyl]glycyl]glycine);and DCTA is a cyclohexan
- R 3 may by (C,-C 4 )alkyl or CH 2 CO 2 -, which may be attached through positions 4 or 5, or through the group R 3 and which carries from 1 to 4 detectable metal or nonmetal cations (M), monovalent cations, or the alkaline earth metals.
- M metal or nonmetal cations
- each individual cyclohexane-based molecule may carry up to 4 metal cations (where both R 3 groups are CH 2 COOM).
- the number of metals will decrease to 2 or even 1 per cyclohexane skeleton. This formula is not intended to limit the molecule to any specific stereochemistry.
- NTA, HEDTA, and DCTA are disclosed in Poster Sessions,
- Bifunctional chelators i.e., chelating groups
- macrocyclic ligands in which conjugation is via an activated arm attached to the carbon backbone of the ligand can also be employed as a chelating group, as described by M.
- the diagnostic chelator or diagnostic chelating groups can be any of the chelating groups disclosed in Scientific Papers, Proceedings of the 46th Annual Meeting, J. Nuc. Med.. Wednesday, June 9, 1999, p. 124, No. 500.
- the chelating group can be any one of the carbonyl complexes disclosed in Waibel et al., Nature Biotechnology, 897-901, Vol. 17, September 1999; or Sattelberger et al., Nature Biotechnology, 849-850, Vol. 17, September 1999.
- the detectable chelating group can be any of the carbonyl complexes disclosed in Waibel et al., Nature Biotechnology, 897-901, Vol. 17, September 1999; or Sattelberger et al, Nature Biotechnology, 849-850, Vol. 17, September 1999, further comprising a metallic radionuclide. More specifically, the detectable chelating group can be any of the carbonyl complexes disclosed in Waibel et al., Nature Biotechnology, 897-901, Vol. 17, September 1999; or Sattelberger et al., Nature Biotechnology, 849-850, Vol. 17, September 1999, further comprising Technetium-99m.
- the detectable chelating group can be any of the carbonyl complexes disclosed in Waibel et al., Nature Biotechnology, 897-901, Vol. 17, September 1999; or Sattelberger et al, Nature Biotechnology. 849-850, Vol. 17, September 1999, further comprising a metallic radionuclide. More specifically, the detectable chelating group can be any of the carbonyl complexes disclosed in Waibel et al., Nature Biotechnology, 897-901, Vol. 17, September 1999; or Sattelberger et al., Nature Biotechnology, 849-850, Vol. 17, September 1999, further comprising Rhenium-186 or Rhenium-188.
- a "detectable radionuclide” is any suitable radionuclide (i.e., radioisotope) capable of being detected in a diagnostic procedure in vivo or in vitro.
- Suitable detectable radionuclides include metallic radionuclides (i.e., metallic radioisotopes) and non-metallic radionuclides (i.e., non-metallic radioisotopes).
- Suitable metallic radionuclides include Antimony-124, Antimony-125, Arsenic-74, Barium-103, Barium-140, Beryllium-7, Bismuth-206, Bismuth-207, Cadmium- 109, Cadmium-115m, Calcium-45, Cerium-139, Cerium-141, Cerium-144, Cesium-137, Chromium-51, Cobalt-55, Cobalt-56, Cobalt-57, Cobalt-58, Cobalt-60, Cobalt-64, Copper-67, Erbium- 169, Europium- 152, Gallium-64, Gallium-68, Gadolinium- 153, Gadolinium- 157 Gold-195, Gold-199, Hafhium- 175, Hafiiium- 175-181, Holmium-166, Indium-110, Indium-I ll, Iridium-192, Iron-55, Iron-59,
- the compounds of the invention can also comprise one or more (e.g., 1, 2, 3, or 4) non-metallic radionuclide which can be directly linked to a residue of the compound of formula I at any synthetically feasible site, or can be linked to a residue of the compound of formula I, by a linker, at any synthetically feasible site.
- Suitable linkers are described herein.
- suitable points of attachment of a the compound of formula I for the non-metallic radionuclide, either directly or by a linker are also described herein.
- the invention also provides compounds having more than one non-metallic radionuclide attached to a compound of formula I, either directly, or by a linker.
- the non-metallic radionuclide can be a non-metallic paramagnetic atom (e.g., Fluorine- 19); or a non-metallic positron emitting radionuclide (e.g., Carbon-11, Fluorine-18, Iodine-123, or Bromine-76).
- Fluorine-18 is a suitable non-metallic radionuclide for use the compounds of the present invention in part because there is typically little or no background noise associated with the diagnostic use of fluorine in the body of a mammal (e.g., human).
- the detectable radionuclide is a non-metallic radionuclide, e.g., Carbon-11, Fluorine-18, Bromine-76, Iodine-123, Iodine-124.
- the compounds disclosed herein can be prepared using procedures similar to those described in U.S. Patent Number 5,739,313, or using procedures similar to those described herein.
- the residue of a molecules comprising B-10 can be linked to the residue of a compound of formula I as described herein. Additional compounds, intermediates, and synthetic preparations thereof are disclosed, for example, in Hogenkamp, H. et al., Synthesis and Characterization of nido-Carborane-Cobalamin Conjugates, Nucl. Med.
- a specific compound of the present invention is compound wherein a residue of the compound of formula I is linked directly or by a linker to a residue of a chemotherapeutic agent; wherein X is CN; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is compound wherein a residue of the compound of formula I is linked directly or by a linker to a residue of a chemotherapeutic agent; wherein the compound of formula I is linked directly or by a linker to a detectable radionuclide; wherein X is CN; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of a chemotherapeutic agent is linked directly or by a linker to a residue of the b-, d-, or e- carboxamide of a compound of formula I; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of a chemotherapeutic agent is linked directly or by a linker to a residue of the b-, d-, or e- carboxamide of a compound of formula I; wherein a detectable radionuclide is linked directly or by a linker to a residue of the b-, d-, or e- carboxamide of a compound of formula I; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of a compound of formula I, wherein X is CN is linked directly or by a linker to a residue of an antineoplastic agent; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of a compound of formula I, wherein X is CN is linked directly or by a linker to a residue of an antineoplastic agent; wherein a residue of a compound of formula I is linked directly or by a linker to a detectable radionuclide; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of a compound of formula I, wherein X is CN is linked directly or by a linker to a residue of paclitaxel or doxorubicin; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of a compound of formula I, wherein X is CN is linked directly or by a linker to a residue of paclitaxel or doxorubicin; wherein a residue of a compound of formula I is linked directly or by a linker to a detectable radionuclide; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of the compound of formula I is linked directly or by a linker to a residue of paclitaxel or doxorubicin at the b-, d-, or e- carboxamide; or a pharmaceutically acceptable salt thereof.
- Another specific compound of the present invention is a compound wherein a residue of the compound of formula I is linked directly or by a linker to a residue of paclitaxel or doxorubicin at the b-, d-, or e- carboxamide; wherein a residue of a compound of formula I is linked directly or by a linker to a detectable radionuclide; or a pharmaceutically acceptable salt thereof.
- salts are organic acid addition salts formed with acids which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate, citrate, malonate, tartarate, succinate, benzoate, ascorbate, ⁇ -ketoglutarate, and ⁇ -glycerophosphate.
- Suitable inorganic salts may also be formed, including, sulfate, nitrate, bicarbonate, and carbonate salts.
- salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion.
- a sufficiently basic compound such as an amine
- a suitable acid affording a physiologically acceptable anion.
- Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made.
- the present invention provides a method of treating a tumor in a mammal.
- the tumor can be located in any part of the mammal.
- the tumor can be located in the breast, lung, thyroid, lymph node, genitourinary system (e.g., kidney, ureter, bladder, ovary, teste, or prostate), musculoskeletal system (e.g., bones, skeletal muscle, or bone marrow), gastrointestinal tract (e.g., stomach, esophagus, small bowel, colon, rectum, pancreas, liver, or smooth muscle), central or peripheral nervous system (e.g., brain, spinal cord, or nerves), head and neck tumors (e.g., ears, eyes, nasopharynx, oropharynx, or salivary glands), or the heart.
- genitourinary system e.g., kidney, ureter, bladder, ovary, teste, or prostate
- musculoskeletal system e.g.,
- the compound of the present invention can be formulated as pharmaceutical compositions and administered to a mammalian host, such as a human patient in a variety of forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, or subcutaneous routes.
- the cobalamin conjugates may be systemically administered, e.g., orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier. They may be enclosed in hard or soft shell gelatin capsules, may be compressed into tablets, or may be incorporated directly with the food of the patient's diet.
- a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier.
- the substance may be combined with one or more excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like.
- Such compositions and preparations should contain at least 0.1% of the substance.
- the percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60% of the weight of a given unit dosage form. The amount of substance in such therapeutically useful compositions is such that an effective dosage level will be obtained.
- the tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added.
- a liquid carrier such as a vegetable oil or a polyethylene glycol.
- any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non-toxic in the amounts employed.
- the substance may be incorporated into sustained-release preparations and devices.
- the cobalamin conjugates can also be administered intravenously or intraperitoneally by infusion or injection.
- Solutions of the substance can be prepared in water, optionally mixed with a nontoxic surfactant.
- Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
- the pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the substance which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes.
- the liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, normal saline, ethanol, a polyol (for example, glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof.
- the proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants.
- the prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin.
- Sterile injectable solutions are prepared by incorporating the substance in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filter sterilization.
- the preferred methods of preparation are vacuum drying and the freeze drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions.
- Useful dosages of the compounds of formula I can be determined by comparing their in vitro activity, and in vivo activity in animal models. Methods for the extrapolation of effective dosages in mice, and other animals, to humans are known to the art; for example, see U.S. Pat. No. 4,938,949.
- the amount of the substance required for use in treatment will vary not only with the particular salt selected but also with the route of administration, the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician.
- a suitable dose will be in the range of from about 0.5 to about 100 mg/kg, e.g., from about 10 to about 75 mg/kg of body weight per day, such as 3 to about 50 mg per kilogram body weight of the recipient per day, preferably in the range of 6 to 90 mg/kg/day, most preferably in the range of 15 to 60 mg/kg/day.
- the substance is conveniently administered in unit dosage form; for example, containing 5 to 1000 mg, conveniently 10 to 750 mg, most conveniently, 50 to 500 mg of active ingredient per unit dosage form.
- the substance should be administered to achieve peak plasma concentrations of from about 0.5 to about 75 ⁇ M, preferably, about 1 to 50 ⁇ M, most preferably, about 2 to about 30 ⁇ M. This may be achieved, for example, by the intravenous injection of a 0.05 to 5% solution of the substance, optionally in saline, or orally administered as a bolus containing about 1-100 mg of the substance. Desirable blood levels may be maintained by continuous infusion to provide about 0.01-5.0 mg/kg/hr or by intermittent infusions containing about 0.4-15 mg/kg of the substance.
- the substance may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example, as two, three, four or more sub-doses per day.
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Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00922210A EP1231942A1 (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as imaging agents and as antitumor agents |
MXPA02003771A MXPA02003771A (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as imaging agents and as antitumor agents. |
AU42434/00A AU4243400A (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as imaging agents and as antitumor agents |
CA002387757A CA2387757A1 (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as imaging agents and as antitumor agents |
JP2001531420A JP2003512338A (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as contrast agents and antitumor agents |
KR1020027004683A KR20020059618A (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as imaging agents and as antitumor agents |
US10/859,865 US20050004010A1 (en) | 1999-10-15 | 2004-06-03 | Cobalamin conjugates useful as imaging agents and as antitumor agents |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US15987499P | 1999-10-15 | 1999-10-15 | |
US60/159,874 | 1999-10-15 |
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US69019800A Continuation | 1999-10-15 | 2000-10-16 |
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WO2001028592A1 true WO2001028592A1 (en) | 2001-04-26 |
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Application Number | Title | Priority Date | Filing Date |
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PCT/US2000/010097 WO2001028592A1 (en) | 1999-10-15 | 2000-04-15 | Cobalamin conjugates useful as imaging agents and as antitumor agents |
Country Status (8)
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US (1) | US20050004010A1 (en) |
EP (1) | EP1231942A1 (en) |
JP (1) | JP2003512338A (en) |
KR (1) | KR20020059618A (en) |
AU (1) | AU4243400A (en) |
CA (1) | CA2387757A1 (en) |
MX (1) | MXPA02003771A (en) |
WO (1) | WO2001028592A1 (en) |
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002100428A1 (en) * | 2001-06-11 | 2002-12-19 | Transition Therapeutics Inc. | Combination therapies using vitamin b12 and therapeutic agents for treatment of viral, proliferative and inflammatory diseases |
WO2003030929A1 (en) * | 2001-10-05 | 2003-04-17 | Transition Therapeutics Inc. | Combination therapies using methyl donors or methyl donor enhancers and therapeutic agents for treatment of viral, proliferative and inflammatory diseases |
WO2004012774A1 (en) * | 2002-08-02 | 2004-02-12 | Cellgate, Inc. | Conjugates of porphyrin compounds with chemotherapeutic agents |
EP1504010A1 (en) * | 2002-05-15 | 2005-02-09 | Endocyte, Inc. | Vitamin-mitomycin conjugates |
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US7141233B2 (en) | 1995-11-13 | 2006-11-28 | Mayo Foundation For Medical Education And Research | Radionuclide labeling of vitamin B12 and coenzymes thereof |
US7179445B2 (en) | 1999-10-15 | 2007-02-20 | Mayo Foundation For Medical Education And Research | Cobalamin conjugates useful as imaging and therapeutic agents |
JP2007515457A (en) * | 2003-12-22 | 2007-06-14 | ソリダゴ・アーゲー | Cobalamin derivatives effective for diagnosis and treatment of abnormal cell proliferation |
WO2007022494A3 (en) * | 2005-08-19 | 2007-09-07 | Endocyte Inc | Multi-drug ligand conjugates |
EP2139469A2 (en) * | 2007-03-19 | 2010-01-06 | Inflabloc Pharmaceuticals, Inc. | Cobalamin taxane bioconjugates |
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US10080805B2 (en) | 2012-02-24 | 2018-09-25 | Purdue Research Foundation | Cholecystokinin B receptor targeting for imaging and therapy |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6806363B1 (en) * | 1999-04-16 | 2004-10-19 | Mayo Foundation For Medical Education & Research | Cobalamin conjugates useful as antitumor agents |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0220030A2 (en) * | 1985-10-10 | 1987-04-29 | Biotechnology Australia Pty. Ltd. | Oral delivery system |
WO1994027613A1 (en) * | 1993-05-24 | 1994-12-08 | Biotech Australia Pty. Limited | Vitamin b12 mediated oral delivery systems for gcsf and epo |
WO1996004016A1 (en) * | 1994-07-29 | 1996-02-15 | Amgen Inc. | Conjugates of vitamin b12 and proteins |
WO1997018231A1 (en) * | 1995-11-13 | 1997-05-22 | Mayo Foundation For Medical Education And Research | Radionuclide labeling of vitamin b12 and coenzymes thereof |
WO1998008859A1 (en) * | 1996-08-27 | 1998-03-05 | University Of Utah Research Foundation | Bioconjugates and delivery of bioactive agents |
WO2000062808A2 (en) * | 1999-04-16 | 2000-10-26 | Mayo Foundation For Medical Education And Research | Cobalamin conjugates useful as antitumor agents |
WO2000074721A1 (en) * | 1999-06-02 | 2000-12-14 | Biotech Australia Pty Limited | Vitamin directed dual targeting therapy |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3936440A (en) * | 1974-05-22 | 1976-02-03 | Drexel University | Method of labeling complex metal chelates with radioactive metal isotopes |
US3981863A (en) * | 1975-02-25 | 1976-09-21 | Micromedic Diagonistics, Inc. | Cyanocobalamin derivatives |
US4279859A (en) * | 1977-07-21 | 1981-07-21 | Becton Dickinson & Company | Simultaneous radioassay of folate and vitamin B12 |
US4209614A (en) * | 1978-05-30 | 1980-06-24 | E. R. Squibb & Sons, Inc. | Vitamin B12 derivative suitable for radiolabeling |
US4301140A (en) * | 1979-12-14 | 1981-11-17 | G. D. Searle & Co. | Radiopharmaceutical method for monitoring kidneys |
US4283342A (en) * | 1980-01-31 | 1981-08-11 | University Of Delaware | Anticancer agents and methods of manufacture |
DE3262956D1 (en) * | 1981-06-22 | 1985-05-15 | Technicon Instr | Labelled vitamin b12 derivatives, their preparation and use |
US4612302A (en) * | 1983-11-14 | 1986-09-16 | Brigham And Women's Hospital | Clinical use of somatostatin analogues |
US4684620A (en) * | 1984-09-04 | 1987-08-04 | Gibson-Stephens Neuropharmaceuticals, Inc. | Cyclic polypeptides having mu-receptor specificity |
US4853371A (en) * | 1986-06-17 | 1989-08-01 | The Administrators Of The Tulane Educational Fund | Therapeutic somatostatin analogs |
US5807832A (en) * | 1987-06-09 | 1998-09-15 | Biotech Australia Pty Limited | Oral delivery of biologically active substances bound to vitamin B12 |
US5405839A (en) * | 1989-02-28 | 1995-04-11 | Teijin Limited | Vitamin B12 derivative, preparation process thereof, and use thereof |
US4959356A (en) * | 1989-05-26 | 1990-09-25 | The United States Of America As Represented By The United States Department Of Energy | Porphyrins for boron neutron capture therapy |
US5308606A (en) * | 1991-01-30 | 1994-05-03 | The Dow Chemical Company | Method of treating and/or diagnosing soft tissue tumors |
EP1138334A3 (en) * | 1992-06-09 | 2002-04-03 | Neorx Corporation | Pretargeting methods and compounds |
US5286853A (en) * | 1992-09-11 | 1994-02-15 | Boron Biologicals, Inc. | Boron-gadolinium compounds and method of conducting imaging and/or neutron capture therapy with same |
US5449720A (en) * | 1993-05-24 | 1995-09-12 | Biotech Australia Pty Limited | Amplification of the VB12 uptake system using polymers |
US5869465A (en) * | 1994-04-08 | 1999-02-09 | Receptagen Corporation | Methods of receptor modulation and uses therefor |
DE69528523T2 (en) * | 1994-04-08 | 2003-06-12 | Receptagen Corp., Edmonds | RECEPTOR MODULATING AGENT AND CORRESPONDING METHOD |
US5840880A (en) * | 1994-04-08 | 1998-11-24 | Receptagen Corporation | Receptor modulating agents |
CA2170400A1 (en) * | 1994-06-27 | 1996-01-04 | M. Frederick Hawthorne | Boron-containing hormone analogs and methods of their use in imaging or killing cells having hormone receptors |
US5559796A (en) * | 1995-02-28 | 1996-09-24 | National Semiconductor Corporation | Delay control for frame-based transmission of data |
US5877165A (en) * | 1995-11-02 | 1999-03-02 | Brookhaven Science Associates | Boronated porhyrins and methods for their use |
US5936082A (en) * | 1997-12-30 | 1999-08-10 | The University Of Akron | Metallocorrinoids as biologically compatible carriers of pharmacological agents |
US6183723B1 (en) * | 1998-01-21 | 2001-02-06 | Mcw Research Foundation | Transcobalamin mediated transport of vitamins B12 in intrinsic factor or receptor deficient patient |
AUPP405098A0 (en) * | 1998-06-12 | 1998-07-02 | Access Pharmaceuticals Australia Pty Limited | Novel methods of preparation of vitamin b12 derivatives suitable for conjugation to pharmaceuticals |
US6071545A (en) * | 1999-02-03 | 2000-06-06 | Vyrex Corporation | Metallic oligopeptide complexes |
US20020002146A1 (en) * | 2000-02-11 | 2002-01-03 | Halevie-Goldman Brian D. | Compositions and methods for the production of S-adenosylmethionine within the body |
US20020042394A1 (en) * | 2000-05-31 | 2002-04-11 | Hogenkamp Henricus P.C. | Cobalamin compounds useful as antibiotic agents and as imaging agents |
WO2001092283A2 (en) * | 2000-05-31 | 2001-12-06 | Mayo Foundation For Medical Education And Research | Cobalamin compounds useful as cardiovascular agents and as imaging agents |
CA2427146A1 (en) * | 2000-10-25 | 2002-07-18 | Mayo Foundation For Medical Education And Research | Transcobalamin binding conjugates useful for treating abnormal cellular proliferation |
-
2000
- 2000-04-15 JP JP2001531420A patent/JP2003512338A/en active Pending
- 2000-04-15 WO PCT/US2000/010097 patent/WO2001028592A1/en not_active Application Discontinuation
- 2000-04-15 MX MXPA02003771A patent/MXPA02003771A/en not_active Application Discontinuation
- 2000-04-15 KR KR1020027004683A patent/KR20020059618A/en not_active Application Discontinuation
- 2000-04-15 AU AU42434/00A patent/AU4243400A/en not_active Abandoned
- 2000-04-15 CA CA002387757A patent/CA2387757A1/en not_active Abandoned
- 2000-04-15 EP EP00922210A patent/EP1231942A1/en not_active Withdrawn
-
2004
- 2004-06-03 US US10/859,865 patent/US20050004010A1/en not_active Abandoned
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0220030A2 (en) * | 1985-10-10 | 1987-04-29 | Biotechnology Australia Pty. Ltd. | Oral delivery system |
WO1994027613A1 (en) * | 1993-05-24 | 1994-12-08 | Biotech Australia Pty. Limited | Vitamin b12 mediated oral delivery systems for gcsf and epo |
WO1996004016A1 (en) * | 1994-07-29 | 1996-02-15 | Amgen Inc. | Conjugates of vitamin b12 and proteins |
WO1997018231A1 (en) * | 1995-11-13 | 1997-05-22 | Mayo Foundation For Medical Education And Research | Radionuclide labeling of vitamin b12 and coenzymes thereof |
WO1998008859A1 (en) * | 1996-08-27 | 1998-03-05 | University Of Utah Research Foundation | Bioconjugates and delivery of bioactive agents |
WO2000062808A2 (en) * | 1999-04-16 | 2000-10-26 | Mayo Foundation For Medical Education And Research | Cobalamin conjugates useful as antitumor agents |
WO2000074721A1 (en) * | 1999-06-02 | 2000-12-14 | Biotech Australia Pty Limited | Vitamin directed dual targeting therapy |
Non-Patent Citations (2)
Title |
---|
ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, (2000) VOL. 219, NO. 1-2, PP. MEDI 82. MEETING INFO.: 219TH MEETING OF THE AMERICAN CHEMICAL SOCIETY. SAN FRANCISCO, CALIFORNIA, USA MARCH 26-30, 2000 AMERICAN CHEMICAL SOCIETY. * |
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; PINSON, PATRICK R. (1) ET AL: "Synthesis, protein binding, and cellular uptake of doxorubicin - cobalamin bioconjugates.", XP002157413, retrieved from STN * |
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Also Published As
Publication number | Publication date |
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EP1231942A1 (en) | 2002-08-21 |
CA2387757A1 (en) | 2001-04-26 |
AU4243400A (en) | 2001-04-30 |
KR20020059618A (en) | 2002-07-13 |
US20050004010A1 (en) | 2005-01-06 |
JP2003512338A (en) | 2003-04-02 |
MXPA02003771A (en) | 2005-04-28 |
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