WO2001027322A3 - Chitobiase as a reporter enzyme - Google Patents

Chitobiase as a reporter enzyme Download PDF

Info

Publication number
WO2001027322A3
WO2001027322A3 PCT/US2000/021048 US0021048W WO0127322A3 WO 2001027322 A3 WO2001027322 A3 WO 2001027322A3 US 0021048 W US0021048 W US 0021048W WO 0127322 A3 WO0127322 A3 WO 0127322A3
Authority
WO
WIPO (PCT)
Prior art keywords
chitobiase
reporter enzyme
reporter
gene constructs
cytoplasmic form
Prior art date
Application number
PCT/US2000/021048
Other languages
French (fr)
Other versions
WO2001027322A2 (en
Inventor
Judith Zyskind
Original Assignee
Elitra Pharmaceuticals Inc
Judith Zyskind
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitra Pharmaceuticals Inc, Judith Zyskind filed Critical Elitra Pharmaceuticals Inc
Priority to AU67547/00A priority Critical patent/AU6754700A/en
Publication of WO2001027322A2 publication Critical patent/WO2001027322A2/en
Publication of WO2001027322A3 publication Critical patent/WO2001027322A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

The present invention relates to reporter gene constructs encoding a cytoplasmic form of chitobiase (N,N'-diacetylchitobiase) and methods of using these reporter gene constructs. The use of a cytoplasmic form of chitobiase as a reporter enzyme is generally applicable to the study of gene expression in organisms which do not contain N-acetyl-β-D-glucosaminidases.
PCT/US2000/021048 1999-10-13 2000-08-02 Chitobiase as a reporter enzyme WO2001027322A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU67547/00A AU6754700A (en) 1999-10-13 2000-08-02 Chitobiase as a reporter enzyme

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15922199P 1999-10-13 1999-10-13
US60/159,221 1999-10-13

Publications (2)

Publication Number Publication Date
WO2001027322A2 WO2001027322A2 (en) 2001-04-19
WO2001027322A3 true WO2001027322A3 (en) 2001-12-13

Family

ID=22571613

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/021048 WO2001027322A2 (en) 1999-10-13 2000-08-02 Chitobiase as a reporter enzyme

Country Status (2)

Country Link
AU (1) AU6754700A (en)
WO (1) WO2001027322A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103555648B (en) * 2013-08-27 2015-06-17 云南省烟草公司临沧市公司 Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998049320A1 (en) * 1997-04-24 1998-11-05 Her Majesty In Right Of Canada, As Represented By The Minister Of Agrigulture And Agri-Food Canada SECRETED α-AMYLASE AS A REPORTER GENE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998049320A1 (en) * 1997-04-24 1998-11-05 Her Majesty In Right Of Canada, As Represented By The Minister Of Agrigulture And Agri-Food Canada SECRETED α-AMYLASE AS A REPORTER GENE

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; March 1998 (1998-03-01), PIRUZIAN E S ET AL: "The use of a thermostable beta-glucanase gene from Clostridium thermocellum as a reporter gene in plants.", XP002171323, Database accession no. PREV199800227889 *
KALABAT D Y ET AL: "Chitobiase, a new reporter enzyme", BIOTECHNIQUES,US,EATON PUBLISHING, NATICK, vol. 25, no. 6, December 1998 (1998-12-01), pages 1030 - 1035, XP002106689, ISSN: 0736-6205 *
MOLECULAR & GENERAL GENETICS, vol. 257, no. 5, March 1998 (1998-03-01), pages 561 - 567, ISSN: 0026-8925 *

Also Published As

Publication number Publication date
WO2001027322A2 (en) 2001-04-19
AU6754700A (en) 2001-04-23

Similar Documents

Publication Publication Date Title
WO2002016583A3 (en) Constructs and their use in metabolic pathway engineering
AU3246300A (en) Polypeptides having galactose oxidase activity and nucleic acids encoding same
AU8023898A (en) Combined product associating a nucleic acid with a substance breaking up the extracellular matrix for gene therapy
EP2039762A3 (en) Nitralases
WO2001070027A3 (en) Combinations of active ingredients with insecticidal and acaricidal properties
EP2169076A3 (en) Enzymes having alpha amylase activity and methods of use thereof
AU1862197A (en) Genetic sequences encoding flavonoid pathway enzymes and uses therefor
WO2001079462A3 (en) Nucleic acids encoding polypeptides having haloperoxidase activity
CA2185712A1 (en) Enhanced virus-mediated dna transfer
CA2420874A1 (en) Active ingredient combinations with insecticidal and acaricidal properties
AU2001292842A1 (en) Compositions and methods relating to prostate specific genes and proteins
HK1154623A1 (en) Isolated luciferases and the use thereof
AU2001241674A1 (en) Variant galactose oxidase, nucleic acid encoding same, and methods of using same
WO1997020933A3 (en) Mutational variants of mammalian ob gene proteins
AU7489098A (en) Polypeptides having aminopeptidase activity and nucleic acids encoding same
WO1999004011A3 (en) Hiv-1 group o antigens and uses thereof
AU2002245031A1 (en) Compositions and methods relating to prostate specific genes and proteins
WO2003092612A3 (en) VECTORS HAVING BOTH ISOFORMS OF β-HEXOSAMINIDASE
WO2000028043A3 (en) Polypeptides having lactonohydrolase activity and nucleic acids encoding same
WO2001027322A3 (en) Chitobiase as a reporter enzyme
IL129139A0 (en) Vertebrate smoothened proteins
ES2171136A1 (en) Enzyme with proteolytic activity
EP0962533A3 (en) Sugar-responsive enhancers in alpha-amylase genes
WO2001098512A3 (en) Control of metabolism with compositions of the human 2-oxoglutarate carrier
WO2002014345A3 (en) Antimicrobial peptides isolated from mast cells

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ CZ DE DE DK DK DM DZ EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ CZ DE DE DK DK DM DZ EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP