WO2001025477A3 - Kinase and phosphatase assays - Google Patents

Kinase and phosphatase assays Download PDF

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Publication number
WO2001025477A3
WO2001025477A3 PCT/GB2000/003736 GB0003736W WO0125477A3 WO 2001025477 A3 WO2001025477 A3 WO 2001025477A3 GB 0003736 W GB0003736 W GB 0003736W WO 0125477 A3 WO0125477 A3 WO 0125477A3
Authority
WO
WIPO (PCT)
Prior art keywords
kinase
substrate
reporter
treated according
candidate modulator
Prior art date
Application number
PCT/GB2000/003736
Other languages
French (fr)
Other versions
WO2001025477A2 (en
Inventor
Julie Ann Frearson
Original Assignee
Biofocus Discovery Ltd
Julie Ann Frearson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biofocus Discovery Ltd, Julie Ann Frearson filed Critical Biofocus Discovery Ltd
Priority to AU75374/00A priority Critical patent/AU7537400A/en
Publication of WO2001025477A2 publication Critical patent/WO2001025477A2/en
Publication of WO2001025477A3 publication Critical patent/WO2001025477A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for screening for modulators of kinase or phosphatase activity are described. The methods comprise: a) providing a serine or threonine kinase and a substrate which can be phosphorylated by the kinase; b) contacting the kinase with its substrate in the presence and absence of a candidate modulator of the activity of the kinase under conditions which permit phosphorylation of the substrate by the kinase; c) contacting the substrates treated according to step (b) with a reporter, excluding a natural antibody, which binds phosphorylated substrate with higher affinity than unphosphorylated substrate; and d) comparing binding of the reporter to the substrate treated according to step (b) in the presence of the candidate modulator with binding of the reporter to the substrate treated according to step (b) in the absence of the candidate modulator. Examples of reporters for use in such assays include 14-3-3 proteins and WW domain containing proteins.
PCT/GB2000/003736 1999-10-01 2000-09-29 Kinase and phosphatase assays WO2001025477A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75374/00A AU7537400A (en) 1999-10-01 2000-09-29 Assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9923208.4 1999-10-01
GBGB9923208.4A GB9923208D0 (en) 1999-10-01 1999-10-01 Assay

Publications (2)

Publication Number Publication Date
WO2001025477A2 WO2001025477A2 (en) 2001-04-12
WO2001025477A3 true WO2001025477A3 (en) 2002-01-31

Family

ID=10861925

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003736 WO2001025477A2 (en) 1999-10-01 2000-09-29 Kinase and phosphatase assays

Country Status (3)

Country Link
AU (1) AU7537400A (en)
GB (1) GB9923208D0 (en)
WO (1) WO2001025477A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219846A1 (en) * 2002-02-28 2003-11-27 Pfizer Inc. Assay for activity of the ActRIIB kinase
US7195884B2 (en) 2002-07-19 2007-03-27 Promega Corp. Methods and kits for transferases
WO2004033711A2 (en) * 2002-09-11 2004-04-22 Novartis Ag Screening assay for identifying agents that modulate pak kinase or cd45 tyrosine phosphatase activity
GB2402938B (en) * 2003-06-17 2005-11-09 Medical Res Council Kinase assay
WO2005114198A2 (en) * 2004-05-20 2005-12-01 Pharmacopeia, Inc. Protein phosphorylation assay
CA2642201C (en) * 2006-02-13 2014-09-02 Olga Ornatsky Kinase and phosphatase assays conducted by elemental analysis

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541113A (en) * 1993-09-22 1996-07-30 Beckman Instruments, Inc. Method for detecting an analyte using an electrochemical luminescent transition metal label
WO1997039326A2 (en) * 1996-04-18 1997-10-23 Ariad Pharmaceuticals, Inc. In vitro fluorescence polarization assay
WO1998018956A1 (en) * 1996-10-28 1998-05-07 Panvera Corporation Kinase activity measurement using fluorescence polarization
US5798214A (en) * 1994-07-13 1998-08-25 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Of Defence Evaluation And Research Agency Capture asays
GB2327498A (en) * 1997-07-16 1999-01-27 Cancer Res Campaign Tech Purification of ATM, or homologues thereof, and assays involving the same
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO2000048621A2 (en) * 1999-02-18 2000-08-24 Beth Israel Deaconess Medical Center Methods and compositions for regulating protein-protein interactions
WO2000075167A2 (en) * 1999-06-09 2000-12-14 Ljl Biosystems, Inc. Phosphorylation assays

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541113A (en) * 1993-09-22 1996-07-30 Beckman Instruments, Inc. Method for detecting an analyte using an electrochemical luminescent transition metal label
US5798214A (en) * 1994-07-13 1998-08-25 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Of Defence Evaluation And Research Agency Capture asays
WO1997039326A2 (en) * 1996-04-18 1997-10-23 Ariad Pharmaceuticals, Inc. In vitro fluorescence polarization assay
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998018956A1 (en) * 1996-10-28 1998-05-07 Panvera Corporation Kinase activity measurement using fluorescence polarization
GB2327498A (en) * 1997-07-16 1999-01-27 Cancer Res Campaign Tech Purification of ATM, or homologues thereof, and assays involving the same
WO2000048621A2 (en) * 1999-02-18 2000-08-24 Beth Israel Deaconess Medical Center Methods and compositions for regulating protein-protein interactions
WO2000075167A2 (en) * 1999-06-09 2000-12-14 Ljl Biosystems, Inc. Phosphorylation assays

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOEL GABY-FLEUR ET AL: "Adenylyl cyclase type II is stimulated by PKC via C-terminal phosphorylation.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1358, no. 3, 1997, pages 307 - 313, XP000986651, ISSN: 0006-3002 *
CULLERE X ET AL: "SERINE 257 PHOSPHORYLATION REGULATES ASSOCIATION OF POLYOMAVIRUS MIDDLE T ANTIGEN WITH 14-3-3 PROTEINS", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 72, no. 1, January 1998 (1998-01-01), pages 558 - 563, XP000881015, ISSN: 0022-538X *

Also Published As

Publication number Publication date
AU7537400A (en) 2001-05-10
GB9923208D0 (en) 1999-12-08
WO2001025477A2 (en) 2001-04-12

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