WO2001025455A2 - Promoter for regulating expression of foreign genes - Google Patents

Promoter for regulating expression of foreign genes Download PDF

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Publication number
WO2001025455A2
WO2001025455A2 PCT/CA2000/001144 CA0001144W WO0125455A2 WO 2001025455 A2 WO2001025455 A2 WO 2001025455A2 CA 0001144 W CA0001144 W CA 0001144W WO 0125455 A2 WO0125455 A2 WO 0125455A2
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WIPO (PCT)
Prior art keywords
promoter
expression
gene
foreign genes
plant
Prior art date
Application number
PCT/CA2000/001144
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French (fr)
Other versions
WO2001025455A3 (en
Inventor
Louis-Philippe Vézina
Marc-André D'Aoust
Original Assignee
Medicago Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc. filed Critical Medicago Inc.
Priority to NZ517905A priority Critical patent/NZ517905A/en
Priority to AU77650/00A priority patent/AU780999B2/en
Priority to MXPA02003455A priority patent/MXPA02003455A/en
Priority to DE60024658T priority patent/DE60024658T2/en
Priority to JP2001528607A priority patent/JP2003512822A/en
Priority to BRPI0014481A priority patent/BRPI0014481B1/en
Priority to AT00967453T priority patent/ATE312184T1/en
Priority to CA002385348A priority patent/CA2385348C/en
Priority to EP00967453A priority patent/EP1222294B1/en
Publication of WO2001025455A2 publication Critical patent/WO2001025455A2/en
Publication of WO2001025455A3 publication Critical patent/WO2001025455A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Definitions

  • the invention relates to a promoter for regulating expression of foreign genes in a transgenic organism, more specifically in a leaf-specific manner in transgenic plants.
  • the transgenic cell, or a tissue or organism regenerated from the transgenic cell will then perform transcription and translation of the transgene and therefore be enabled to accumulate the protein of interest or to per orm the new metabolic reaction through the activity of the enzyme of interest.
  • the emerging industry of molecular farming is one of the most promising industry of the coming century. Its promise is to provide safe and renewable molecule factories for the industry.
  • Photosynthesis is a metabolic reaction of paramount importance in the living world. It is performed by most land plants and algae, and by some bacteria. This overall reaction involves a complex assembly of electron transfer proteins spatially arranged within the thylakoid membrane system located in the chloroplast of leaf cells.
  • This electron transport chain is coupled at one end with the photosynthetic antennae, which comprise a variety of macro-molecules, including one molecule common to all photosynthetic organism, chlorophyll, and at the other end, to the enzymes involved in NADPH and ATP synthesis, and to the Calvin cycle, involved in coupling the release of energy from NADPH and ATP with the fixation of gaseous carbon dioxide into organic molecules.
  • the photosynthetic antennae comprise a variety of macro-molecules, including one molecule common to all photosynthetic organism, chlorophyll, and at the other end, to the enzymes involved in NADPH and ATP synthesis, and to the Calvin cycle, involved in coupling the release of energy from NADPH and ATP with the fixation of gaseous carbon dioxide into organic molecules.
  • the proteins involved in the overall photosynthetic reaction one, Ribulose biphosphate carboxylase (Rubisco), is the most abundant protein on earth.
  • the peptidic constituents of the photosynthetic apparatus are encoded by genes present in the chloroplastic genome; as an example, the heavy subunit of Rubisco, which bears the catalytic sites for C0 2 fixation, is encoded by a chloroplastic gene.
  • the small subunit of this enzyme is encoded by a nuclear gene, and thus the Rubisco holo-protein is made of subunits encoded by two different genomes.
  • the promoter has been extensively characterized and its use in expression vectors is protected by United States Patent No. 4,962,028.
  • One aim of the present invention is to provide with a promoter for regulating expression of foreign genes in a transgenic organism, more specifically in transgenic plants.
  • a promoter for regulating expression of foreign genes in transgenic organisms which comprises a promoter having the identifying characteristics of a promoter having a sequence selected from the group consisting of sequences set forth in SEQ ID NOS:1 to 3 and functional fragments or derivatives thereof, wherein said promoter is adapted to be operationally located with respect to said foreign gene for expression of said gene.
  • the preferred promoter of the present invention has a sequence selected from the group consisting of sequences set forth in SEQ ID NOS:1 to 3.
  • the organism is a plant.
  • the promoter of the present invention may be modulated by the presence or absence of light.
  • the prefered plant is a dicot, a monocot or a gymnosperm.
  • a method of regulating expression of foreign genes in transgenic organisms comprising the steps of: a) preparing a transgenic organism using an expression construct consisting of at least a promoter of the present invention, and an ORF of a gene, wherein said promoter is operationally located with respect to said gene for expression of said gene.
  • an expression construct consisting of at least a promoter of the present invention, and an ORF of a gene, wherein said promoter is operationally located with respect to said gene for expression of said gene.
  • SEQ ID NOS:1-3 was then ligated to a reporter gene and a terminator, and this construct was inserted in suitable plant expression vectors for DNA bombardment onto alfalfa leaves and for Agrobacterium mediated DNA transfer as described by Desgagnes et al. (1995, Plant Cell Tissue Organ Cult. 42:129-140). These two DNA transfer methods were used to demonstrate that expression of the reporter gene can be modulated by light.
  • DNA sequencing was performed as described by Sanger et al (1977, P.N.A.S. USA, 74:5643-5647).
  • the resulting promoters of the present invention have the sequence as set forth in SEQ ID NOS: 1 to 3.
  • the cassettes for expression analysis using the GUS reporter gene were assembled as follows.
  • a promoterless GUS cassette was digested from pBI101 with Hindlll and EcoRI, and was inserted into the Hindlll and EcoRI sites of the pUC19 polycloning site.
  • the resulting plasmid was named pBI201 and was used for further constructs.
  • Various deletion fragments of pGPIas3-2 were transcriptionally and transitionally fused at the 5'terminus of the GUS reporter gene in pBI201 and these were used for transitory expression studies using DNA bombardment.
  • Upon identification of the adequate deletion fragment it was subcloned into a binary plant expression vector such as pBI101 (Clonetech). These recombinant plasmids were used for stable integration through A. tumefaciens infection as described below.
  • the recombinant plasmids were introduced into Agrobacterium tumefaciens strain LBA4404 by electroporation as described in Khoudi et al (1999, Biotechnol. Bioeng., 64:135-143). Selected Agrobacterium strains were then co-cultivated with leaf disks from genotype C5-1 for 4 days in the absence of selection pressure (kanamycin). Following this incubation period, leaf disks were washed and pampered, and then allowed to form calli onto medium B5H. Calli were then transferred for 21 days on SH medium for embryo induction and for 28 days on BOi2Y for embryo development. Torpedo-shaped embryos were removed from Boi2Y and placed on MS medium for regeneration.
  • Kanamycin was present in all cultivation medium except for co-cultivation and regeneration on MS. This method is described in length in Desgagnes et al (1995, Plant Cell Tissue Organ Cult. 42:129-140). Rooted plantlets were grown to maturity in the greenhouse.

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a promoter for regulating expression of foreign genes in transgenic organisms, which comprises a promoter having the identifying characteristics of a promoter having a sequence selected from the group consisting of sequences set forth in SEQ ID NOS: 1 to 3 and functional fragments or derivatives thereof, wherein said promoter is adapted to be operationally located with respect to said foreign gene for expression of said gene.

Description

PROMOTER FOR REGULATING EXPRESSION OF FOREIGN GENES
BACKGROUND OF THE INVENTION
(a) Field of the Invention The invention relates to a promoter for regulating expression of foreign genes in a transgenic organism, more specifically in a leaf-specific manner in transgenic plants.
(b) Description of Prior Art
Genetic transformation of microbes have been used for more than 15 years to produce useful recombinant molecules, and applications in the pharmaceutical, cosmaceutical and dermaceutical industries are being currently exploited. This technology has expanded from microbes to plants and animals in the last ten years with the development of techniques required to adapt this general concept to complex eukaryotic organisms. Basically a gene encoding for a protein of interest or a gene encoding for an enzyme responsible for a modification of a metabolic pathway that leads to a molecule of interest, is linked in an appropriate fashion to cis-and trans-acting regulatory sequences, and transferred to a target cell where it is incorporated in the molecular machinery (in a transitory or stable fashion). The transgenic cell, or a tissue or organism regenerated from the transgenic cell will then perform transcription and translation of the transgene and therefore be enabled to accumulate the protein of interest or to per orm the new metabolic reaction through the activity of the enzyme of interest. The emerging industry of molecular farming is one of the most promising industry of the coming century. Its promise is to provide safe and renewable molecule factories for the industry. Among the applications that are currently developed are the production of low-cost monoclonal antibodies for therapeutic and diagnostic uses, the production of unlimited amounts of hormones, cytokines and other bio-active molecules for the treatment of chronicle or lethal diseases, the production of bio-safe substitutes for various blood components, the production of unlimited amounts of processing enzymes for the food and pulp industry, the production of low-cost enzymes for waste treatments, and the production of safe bio-active molecules for the cosmetic industry. Limitations to the application of this technology has often come from the inability of transgenic organisms to accumulate adequate amounts of the recombinant product, as a result of low transcription rates, improper splicing of the messenger, instability of the foreign mRNA, poor translation rates, hyper-susceptibility of the recombinant protein to the action of endogenous proteases or hyper-susceptibility of the recombinant organism to the foreign protein which result in improper and limited growth or in the worst cases, in strong deleterious effects to the host organism. Inadequacy of production level has a direct impact on the development of applications when profit margins are narrow, or when treatment and/or disposal of residual matter causes bio-safety or environmental problems. Improvement of the accumulation level of the desired recombinant product thus appears to be one critical factor that warrants commercialization of many applications of molecular farming. Photosynthesis is a metabolic reaction of paramount importance in the living world. It is performed by most land plants and algae, and by some bacteria. This overall reaction involves a complex assembly of electron transfer proteins spatially arranged within the thylakoid membrane system located in the chloroplast of leaf cells. This electron transport chain is coupled at one end with the photosynthetic antennae, which comprise a variety of macro-molecules, including one molecule common to all photosynthetic organism, chlorophyll, and at the other end, to the enzymes involved in NADPH and ATP synthesis, and to the Calvin cycle, involved in coupling the release of energy from NADPH and ATP with the fixation of gaseous carbon dioxide into organic molecules. Among the proteins involved in the overall photosynthetic reaction, one, Ribulose biphosphate carboxylase (Rubisco), is the most abundant protein on earth.
Photosynthesis is thus what leaf cells are dedicated to perform, and there is an obvious interest to use promoters of genes involved in such prominent tissue-specific metabolic activity when building strong leaf- specific expression cassettes for applications in plant biotechnology.
Many of the peptidic constituents of the photosynthetic apparatus are encoded by genes present in the chloroplastic genome; as an example, the heavy subunit of Rubisco, which bears the catalytic sites for C02 fixation, is encoded by a chloroplastic gene. However, the small subunit of this enzyme is encoded by a nuclear gene, and thus the Rubisco holo-protein is made of subunits encoded by two different genomes. For obvious reasons, there has been a great interest in trying to use Rubisco promoters to control transcription of transgenes in leaves of transgenic plants. The promoter has been extensively characterized and its use in expression vectors is protected by United States Patent No. 4,962,028.
It would be highly desirable to be provided with a promoter for regulating expression of foreign genes in a transgenic organism, more specifically in transgenic plants.
SUMMARY OF THE INVENTION
One aim of the present invention is to provide with a promoter for regulating expression of foreign genes in a transgenic organism, more specifically in transgenic plants.
In accordance with one embodiment of the present invention, there is provided a promoter for regulating expression of foreign genes in transgenic organisms, which comprises a promoter having the identifying characteristics of a promoter having a sequence selected from the group consisting of sequences set forth in SEQ ID NOS:1 to 3 and functional fragments or derivatives thereof, wherein said promoter is adapted to be operationally located with respect to said foreign gene for expression of said gene.
The preferred promoter of the present invention has a sequence selected from the group consisting of sequences set forth in SEQ ID NOS:1 to 3.
Preferably, the organism is a plant.
Preferably, the promoter of the present invention may be modulated by the presence or absence of light. The prefered plant is a dicot, a monocot or a gymnosperm.
In accordance with another embodiment of the present invention, there is provided a method of regulating expression of foreign genes in transgenic organisms, comprising the steps of: a) preparing a transgenic organism using an expression construct consisting of at least a promoter of the present invention, and an ORF of a gene, wherein said promoter is operationally located with respect to said gene for expression of said gene. For the purpose of the present invention the following terms are defined below.
The expression "functional fragments or derivatives thereof" is intended to mean any derivative or fragment of sequences SEQ ID NOS: 1-3 which allow for an equivalent level of expression of a foreign gene as the promoter of the present invention set forth in SEQ ID NOS:1-3.
DETAILED DESCRIPTION OF THE INVENTION
Following is a detailed description of the method used to generate transgenic alfalfa lines that can be regulated in their expression of a reporter gene. In this embodiment, a promoter having the sequence set forth in
SEQ ID NOS:1-3 was then ligated to a reporter gene and a terminator, and this construct was inserted in suitable plant expression vectors for DNA bombardment onto alfalfa leaves and for Agrobacterium mediated DNA transfer as described by Desgagnes et al. (1995, Plant Cell Tissue Organ Cult. 42:129-140). These two DNA transfer methods were used to demonstrate that expression of the reporter gene can be modulated by light.
Materials and Methods
DNA sequencing
DNA sequencing was performed as described by Sanger et al (1977, P.N.A.S. USA, 74:5643-5647).
The resulting promoters of the present invention have the sequence as set forth in SEQ ID NOS: 1 to 3.
Construction of expression cassettes and vectors
The cassettes for expression analysis using the GUS reporter gene were assembled as follows. A promoterless GUS cassette was digested from pBI101 with Hindlll and EcoRI, and was inserted into the Hindlll and EcoRI sites of the pUC19 polycloning site. The resulting plasmid was named pBI201 and was used for further constructs. Various deletion fragments of pGPIas3-2 were transcriptionally and transitionally fused at the 5'terminus of the GUS reporter gene in pBI201 and these were used for transitory expression studies using DNA bombardment. Upon identification of the adequate deletion fragment, it was subcloned into a binary plant expression vector such as pBI101 (Clonetech). These recombinant plasmids were used for stable integration through A. tumefaciens infection as described below.
Agrobacterium-med'iated DNA transfer and regeneration of transgenic lines
The recombinant plasmids were introduced into Agrobacterium tumefaciens strain LBA4404 by electroporation as described in Khoudi et al (1999, Biotechnol. Bioeng., 64:135-143). Selected Agrobacterium strains were then co-cultivated with leaf disks from genotype C5-1 for 4 days in the absence of selection pressure (kanamycin). Following this incubation period, leaf disks were washed and pampered, and then allowed to form calli onto medium B5H. Calli were then transferred for 21 days on SH medium for embryo induction and for 28 days on BOi2Y for embryo development. Torpedo-shaped embryos were removed from Boi2Y and placed on MS medium for regeneration. Kanamycin was present in all cultivation medium except for co-cultivation and regeneration on MS. This method is described in length in Desgagnes et al (1995, Plant Cell Tissue Organ Cult. 42:129-140). Rooted plantlets were grown to maturity in the greenhouse.
While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any varia- tions, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth, and as follows in the scope of the appended claims.

Claims

WHAT IS CLAIMED IS:
1. A promoter for regulating expression of foreign genes in transgenic organisms, which comprises a promoter having the identifying characteristics of a promoter having a sequence selected from the group consisting of sequences set forth in SEQ ID NOS:1 to 3 and functional fragments or derivatives thereof, wherein said promoter is adapted to be operationally located with respect to said foreign gene for expression of said gene.
2. The promoter of claim 1 , wherein said promoter is modulated for transcriptional expression of said gene by presence or absence of light
3. The promoter of claim 1 , wherein the promoter has a sequence selected from the group consisting of sequences set forth in SEQ ID NOS:1 to 3.
4. The promoter of claim 1 , wherein said organism is a plant.
5. The promoter of claim 4, wherein said plant is a dicot, a monocot or a gymnosperm.
6. A method of regulating expression of foreign genes in transgenic organisms, comprising the steps of: a) preparing a transgenic organism using an expression construct consisting of at least a promoter of claim 1 , and an ORF of a gene, wherein said promoter is operationally located with respect to said gene for expression of said gene.
7. The method of claim 6, wherein said organism is a plant.
8. The method of claim 7, wherein said plant is a dicot, a monocot or a gymnosperm.
PCT/CA2000/001144 1999-10-04 2000-10-02 Promoter for regulating expression of foreign genes WO2001025455A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
NZ517905A NZ517905A (en) 1999-10-04 2000-10-02 Promoter modulated in the presence or absence of light for regulating expression of foreign genes in transgenic plants
AU77650/00A AU780999B2 (en) 1999-10-04 2000-10-02 Promoter for regulating expression of foreign genes
MXPA02003455A MXPA02003455A (en) 1999-10-04 2000-10-02 Promoter for regulating expression of foreign genes.
DE60024658T DE60024658T2 (en) 1999-10-04 2000-10-02 PROMOTER FOR REGULATING FOREIGN GENE EXPRESSION
JP2001528607A JP2003512822A (en) 1999-10-04 2000-10-02 Promoter that regulates exogenous gene expression
BRPI0014481A BRPI0014481B1 (en) 1999-10-04 2000-10-02 construct and use to regulate the expression of an exogenous gene in a transgenic plant
AT00967453T ATE312184T1 (en) 1999-10-04 2000-10-02 PROMOTER FOR REGULATION OF FOREIGN GENE EXPRESSION
CA002385348A CA2385348C (en) 1999-10-04 2000-10-02 Promoter for regulating expression of foreign genes
EP00967453A EP1222294B1 (en) 1999-10-04 2000-10-02 Promoter for regulating expression of foreign genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15712999P 1999-10-04 1999-10-04
US60/157,129 1999-10-04

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WO2001025455A2 true WO2001025455A2 (en) 2001-04-12
WO2001025455A3 WO2001025455A3 (en) 2001-11-08

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EP (1) EP1222294B1 (en)
JP (3) JP2003512822A (en)
KR (1) KR100817660B1 (en)
CN (1) CN1377418A (en)
AT (1) ATE312184T1 (en)
AU (1) AU780999B2 (en)
BR (1) BRPI0014481B1 (en)
CA (1) CA2385348C (en)
DE (1) DE60024658T2 (en)
DK (1) DK1222294T3 (en)
ES (1) ES2254235T3 (en)
MX (1) MXPA02003455A (en)
NZ (1) NZ517905A (en)
WO (1) WO2001025455A2 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002036786A2 (en) * 2000-10-31 2002-05-10 Medicago Inc. Method of selecting plant promoters to control transgene expression
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
WO2007016276A2 (en) * 2005-07-27 2007-02-08 J.R. Simplot Company Marker-free plant transformation
US7230161B2 (en) * 2003-06-23 2007-06-12 Pioneer Hi-Bred International, Inc. Engineering single-gene-controlled staygreen potential into plants utilizing ACC synthase from maize
US7598430B2 (en) 2002-03-20 2009-10-06 J.R. Simplot Company Refined plant transformation
US8111991B2 (en) 2001-05-07 2012-02-07 Aurora Networks Inc. N-way broadcast / narrowcast combiner
WO2012098119A2 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Protein expression in plants
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants

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KR100817660B1 (en) * 1999-10-04 2008-03-27 메디카고 인코포레이티드 Promoter for regulating expression of foreign genes
US20110008837A1 (en) * 2007-06-15 2011-01-13 Marc-Andre D-Aoust Modifying glycoprotein production in plans
CN103131712B (en) * 2013-03-08 2016-04-27 沈阳药科大学 A kind of have the photoinduction promoter of startup clover plastocyanin specifically expressing and the primer of a pair this promotor of quick clone

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WO2000056906A1 (en) * 1999-03-22 2000-09-28 Meristem Therapeutics Chimeric promoters based on the plastocyanin pete promoter from pea

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KR100817660B1 (en) * 1999-10-04 2008-03-27 메디카고 인코포레이티드 Promoter for regulating expression of foreign genes

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WO2000056906A1 (en) * 1999-03-22 2000-09-28 Meristem Therapeutics Chimeric promoters based on the plastocyanin pete promoter from pea

Non-Patent Citations (2)

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KENG-HOCK PWEE ET AL: "THE PEA PLASTOCYANIN PROMOTER DIRECTS CELL-SPECIFIC BUT NOT FULL LIGHT-REGULATED EXPRESSION IN TRANSGENIC TOBACCO PLANTS" PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 3, no. 3, 1993, pages 437-449, XP002032746 ISSN: 0960-7412 *
LAST D I ET AL: "PLASTOCYANIN IS ENCODED BY A SINGLE-COPY GENE IN THE PEA HAPLOID GENOME" PLANT MOLECULAR BIOLOGY,NL,NIJHOFF PUBLISHERS, DORDRECHT, vol. 12, 1989, pages 655-666, XP002032748 ISSN: 0167-4412 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
WO2002036786A3 (en) * 2000-10-31 2002-09-26 Medicago Inc Method of selecting plant promoters to control transgene expression
WO2002036786A2 (en) * 2000-10-31 2002-05-10 Medicago Inc. Method of selecting plant promoters to control transgene expression
US8111991B2 (en) 2001-05-07 2012-02-07 Aurora Networks Inc. N-way broadcast / narrowcast combiner
US7598430B2 (en) 2002-03-20 2009-10-06 J.R. Simplot Company Refined plant transformation
US7928292B2 (en) 2002-03-20 2011-04-19 J.R. Simplot Company Refined plant transformation
US7763773B2 (en) 2003-06-23 2010-07-27 Pioneer Hi-Bred International Inc. Engineering single-gene-controlled staygreen potential into plants
US7838730B2 (en) 2003-06-23 2010-11-23 Pioneer Hi-Bred International Inc. Engineering single-gene-controlled staygreen potential into plants
US7230161B2 (en) * 2003-06-23 2007-06-12 Pioneer Hi-Bred International, Inc. Engineering single-gene-controlled staygreen potential into plants utilizing ACC synthase from maize
US8124860B2 (en) 2003-06-23 2012-02-28 Pioneer Hi-Bred International Inc. Zea mays seeds and plants with reduced expression of the ACS6 gene
US8129587B2 (en) 2003-06-23 2012-03-06 Pioneer Hi-Bred International, Inc. Zea mays seeds and plants with reduced expression of the ACS2 gene
US8779235B2 (en) 2003-06-23 2014-07-15 Pioneer Hi-Bred International, Inc. Engineering single-gene-controlled staygreen potential into plants
US7449335B2 (en) 2005-07-27 2008-11-11 J.R. Simplot Company Precise cutting
WO2007016276A3 (en) * 2005-07-27 2008-04-17 Simplot Co J R Marker-free plant transformation
WO2007016276A2 (en) * 2005-07-27 2007-02-08 J.R. Simplot Company Marker-free plant transformation
WO2012098119A2 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Protein expression in plants
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants

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NZ517905A (en) 2004-02-27
AU7765000A (en) 2001-05-10
DE60024658T2 (en) 2006-08-24
ES2254235T3 (en) 2006-06-16
BRPI0014481B1 (en) 2015-12-08
KR20020057968A (en) 2002-07-12
KR100817660B1 (en) 2008-03-27
WO2001025455A3 (en) 2001-11-08
MXPA02003455A (en) 2002-10-23
CA2385348A1 (en) 2001-04-12
BR0014481A (en) 2002-06-04
JP2003512822A (en) 2003-04-08
DE60024658D1 (en) 2006-01-12
CA2385348C (en) 2008-12-02
ATE312184T1 (en) 2005-12-15
CN1377418A (en) 2002-10-30
JP5066289B2 (en) 2012-11-07
EP1222294B1 (en) 2005-12-07
JP2012095653A (en) 2012-05-24
AU780999B2 (en) 2005-04-28
JP2011142925A (en) 2011-07-28
DK1222294T3 (en) 2006-04-18
EP1222294A2 (en) 2002-07-17

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